A novel small regulatory RNA enhances cell motility in enterohemorrhagic Escherichia coli

2014 ◽  
Vol 60 (1) ◽  
pp. 44-50 ◽  
Author(s):  
Naoki Sudo ◽  
Akiko Soma ◽  
Akira Muto ◽  
Sunao Iyoda ◽  
Mayumi Suh ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Motility ◽  
Enterohemorrhagic Escherichia Coli ◽  
Regulatory Rna ◽  
Small Regulatory Rna

In silico ‘fishing’ using known small regulatory RNA (sRNA) candidates as the decoy from Escherichia coli, Salmonella typhi and Salmonella typhimurium manifested 14 novel sRNA candidates in the orthologous region of Proteus mirabilis

2018 ◽  
Vol 45 (6) ◽  
pp. 2333-2343 ◽  
Author(s):  
Selvaraju KishanRaj ◽  
Samuggam Sumitha ◽  
Balakrishnan Siventhiran ◽  
Othayakumar Thiviyaa ◽  
Kathiresan V. Sathasivam ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Salmonella Typhimurium ◽  
Proteus Mirabilis ◽  
In Silico ◽  
Salmonella Typhi ◽  
Regulatory Rna ◽  
Orthologous Region ◽  
Small Regulatory Rna

Mo1770 Expression of the Oxys Small Regulatory RNA in Luminal Commensal Escherichia coli Attenuates Experimental Colitis

2012 ◽  
Vol 142 (5) ◽  
pp. S-681
Author(s):  
Sandrine Y. Tchaptchet ◽  
Ting-Jia Fan ◽  
Laura E. Goeser ◽  
Ryan B. Sartor ◽  
Jonathan J. Hansen
Keyword(s):  
Escherichia Coli ◽  
Experimental Colitis ◽  
Regulatory Rna ◽  
Small Regulatory Rna

scholarly journals Targeting of csgD by the small regulatory RNA RprA links stationary phase, biofilm formation and cell envelope stress in Escherichia coli

2012 ◽  
Vol 84 (1) ◽  
pp. 51-65 ◽  
Author(s):  
Franziska Mika ◽  
Susan Busse ◽  
Alexandra Possling ◽  
Janine Berkholz ◽  
Natalia Tschowri ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Stationary Phase ◽  
Biofilm Formation ◽  
Cell Envelope ◽  
Regulatory Rna ◽  
Small Regulatory Rna ◽  
Envelope Stress

The Escherichia Coli Small Regulatory Rna Oxys Impairs Bacterial Translocation and Attenuates Experimental Colitis

2017 ◽  
Vol 152 (5) ◽  
pp. S1000
Author(s):  
Diana Arsene ◽  
Sandrine Y. Tchaptchet ◽  
Ting-Jia Fan ◽  
Jonathan J. Hansen
Keyword(s):  
Escherichia Coli ◽  
Bacterial Translocation ◽  
Experimental Colitis ◽  
Regulatory Rna ◽  
Small Regulatory Rna

scholarly journals Structure of a bacterial ribonucleoprotein complex central to the control of cell envelope biogenesis

2022 ◽  
Author(s):  
Ben F Luisi ◽  
Md. Saiful Islam ◽  
Steven William Hardwick ◽  
Laura Quell ◽  
Dimitri Y Chirgadze ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bacterial Cell ◽  
Quaternary Structure ◽  
Cell Envelope ◽  
Evolutionary Relationship ◽  
Regulatory Rna ◽  
Rnase E ◽  
Small Regulatory Rna ◽  
Conserved Domain ◽  
Transcription Networks

The biogenesis of the essential precursor of the bacterial cell envelope, glucosamine-6-phosphate (GlcN6P), is controlled through intricate post-transcription networks mediated by GlmZ, a small regulatory RNA (sRNA). GlmZ stimulates translation of the mRNA encoding GlcN6P synthetase in Escherichia coli, but when bound by the protein RapZ, it becomes inactivated through cleavage by the endoribonuclease RNase E. Here we report the cryoEM structure of the RapZ:GlmZ complex, revealing a complementary match of the protein tetrameric quaternary structure to an imperfect structural repeat in the RNA. The RNA is contacted mostly through a highly conserved domain of RapZ that shares deep evolutionary relationship with phosphofructokinase and suggests links between metabolism and riboregulation. We also present the structure of a pre-cleavage encounter intermediate formed between the binary RapZ:GlmZ complex and RNase E that reveals how GlmZ is presented and recognised for cleavage. The structures suggest how other encounter complexes might guide recognition and action of endoribonucleases on target transcripts, and how structured substrates in polycistronic precursors are recognised for processing.

scholarly journals Constitutive Activation of the Escherichia coli Pho Regulon Upregulates rpoS Translation in an Hfq-Dependent Fashion

2003 ◽  
Vol 185 (20) ◽  
pp. 5984-5992 ◽  
Author(s):  
Natividad Ruiz ◽  
Thomas J. Silhavy
Keyword(s):  
Escherichia Coli ◽  
Exponential Growth ◽  
Pho Regulon ◽  
Regulatory Rna ◽  
Genetic Screens ◽  
Two Component System ◽  
Component System ◽  
Small Regulatory Rna ◽  
Two Component ◽  
Σ Factor

ABSTRACT Regulation of the σ factor RpoS occurs at the levels of transcription, translation, and protein stability activity, and it determines whether Escherichia coli turns on or off the stationary-phase response. To better understand the regulation of RpoS, we conducted genetic screens and found that mutations in the pst locus cause accumulation of RpoS during exponential growth. The pst locus encodes for the components of the high-affinity transport system for inorganic phosphate (Pi), which is involved in sensing Pi levels in the environment. When the Pst transporter is compromised (either by mutation or by Pi starvation), the two-component system PhoBR activates the transcription of the Pho regulon, a subset of genes that encode proteins for transporting and metabolizing alternative phosphate sources. Our data show that strains carrying mutations which constitutively activate the Pho regulon have increased rpoS translation during exponential growth. This upregulation of rpoS translation is Hfq dependent, suggesting the involvement of a small regulatory RNA (sRNA). The transcription of this yet-to-be-identified sRNA is regulated by the PhoBR two-component system.

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