1230-P: Isolation of Functional Endothelial Progenitor Cells from Human Adipose Tissue

Diabetes ◽  
2021 ◽  
Vol 70 (Supplement 1) ◽  
pp. 1230-P
Author(s):  
CRISTINA CACCIOPPOLI ◽  
SEBASTIO PERRINI ◽  
VALENTINA ANNAMARIA GENCHI ◽  
ROSSELLA DORIA ◽  
GIUSEPPE PALMA ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Human Adipose Tissue ◽  
Endothelial Progenitor

scholarly journals Isolation of endothelial progenitor cells from human adipose tissue

2016 ◽  
Vol 3 (05) ◽  
pp. 645-652 ◽  
Author(s):  
Phuc Van Pham ◽  
Ngoc Bich Vu ◽  
Hoa Trong Nguyen ◽  
Ngoc Kim Phan
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Human Adipose Tissue ◽  
Vascular Medicine ◽  
Endothelial Progenitor ◽  
Adipose Tissues ◽  
Surface Marker Expression

Adipose tissue is a rich source of stem cells, especially mesenchymal stem cells (MSCs). This study aimed to identify and isolate endothelial progenitor cells (EPCs) from human adipose tissue. Belly adipose tissues were collected from donors with consent. Stromal vascular fractions (SVFs) were extracted from adipose tissues by enzyme collagenase using commercial kits. SVFs were cultured in MSCCult medium for 24 h to obtain MSCs, then supernatant was collected and cell pellet cultured in EGM-2 medium to obtain adipose tissue EPCs (ADEPCs). ADEPCs were checked for surface marker expression of CD31 and VEGFR2, and for angiogenesis capability in vitro. The results showed that SVFs contained a pool of EPCs with strong angiogenesis potential and that adipose tissue is not only a source for MSCs but also for EPCs. Therefore, ADEPCs may a useful source of EPCs for vascular medicine.

scholarly journals Secretome Analysis of Rabbit and Human Mesenchymal Stem and Endothelial Progenitor Cells: A Comparative Study

2021 ◽  
Vol 22 (22) ◽  
pp. 12283
Author(s):  
Jaromír Vašíček ◽  
Andrej Baláži ◽  
Mária Tirpáková ◽  
Andrea Svoradová ◽  
Ľubomír Ondruška ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Large Scale ◽  
Human Adipose Tissue ◽  
Endothelial Progenitor ◽  
Secretome Analysis ◽  
Cytokine Array ◽  
Human Cytokine

Human adipose tissue-derived mesenchymal stem cells (AT-MSCs) have been studied several years for their immunomodulatory effect through the paracrine mechanism and cytokine secretion. In combination with endothelial progenitor cells (EPCs), MSCs have great therapeutical potential for the repair of endothelium and wound healing. However, little is known about the cytokine profile of rabbit AT-MSCs or even EPCs. The aim of this study was to analyze the secretomes of these rabbit stem/progenitor cells. A large-scale human cytokine array (up to 80 cytokines) was used to identify and compare cytokines secreted into conditioned media of human and rabbit AT-MSCs as well as HUVECs and rabbit EPCs. Few cytokines were highly expressed by human AT-MSCs (TIMP-2, TIMP-1), HUVECs (MCP-1, TIMP-2, GRO, Angiogenin, IL-8, TIMP-1), or by rabbit EPCs (TIMP-2). Several cytokines have moderate expression by human (MCP-1, GRO, Angiogenin, TGF-β 2, IL-8, LIF, IL-6, Osteopontin, Osteoprotegerin) and rabbit AT-MSCs (TIMP-2, TGF-β 2, LIF, Osteopontin, IL-8, IL-5, IL-3) or by HUVECs (IL-6, MIF, TGF-β 2, GCP-2, IGFBP-2, Osteoprotegerin, EGF, LIF, PDGF-BB, MCP-3, Osteopontin, Leptin, IL-5, ENA-78, TNF- β) and rabbit EPCs (TGF-β 2, Osteopontin, GRO, LIF, IL-8, IL-5, IL-3). In conclusion, the proposed method seems to be useful for the secretome analysis of rabbit stem/progenitor cells.

scholarly journals Effects of adipose-derived stromal cells and endothelial progenitor cells on adipose transplant survival and angiogenesis

2020 ◽  
Author(s):  
Xian Zhao ◽  
Fengshan Gan ◽  
Liu Liu ◽  
Qingzhu Zhou ◽  
Wenli Huang ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Blood Vessels ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Migration And Invasion ◽  
Vascular Endothelial ◽  
Paracrine Signaling ◽  
Endothelial Progenitor ◽  
Endothelial Markers

Abstract Background: A paracrine mechanism is thought to mediate the proangiogenic capacity of adipose-derived stromal/stem cells (ASCs). However, the precise mechanism by which ASCs promote the formation of blood vessels by endothelial progenitor cells (EPCs) is unclear.Methods: We cocultured ASCs with EPCs at various concentrations to study the effects on angiogenesis. The supernatant from cultured ASCs was cocultured with EPCs to evaluate the effects on the expression of vascular endothelial markers in EPCs, as well as capacity for migration and invasion. We then mixed ASCs with EPCs and transplanted them with adipose tissue into New Zealand white rabbits to evaluate the effects on angiogenesis in adipose tissue grafts.Results: As the relative abundance of ASCs cocultured with EPCs increased, the rate of angiogenesis among EPCs decreased. The supernatant from ASC cultures increased the migration and invasion of EPCs and upregulated the expression of vascular endothelial markers in EPCs. In vivo, ASCs promoted the production of blood vessels by EPCs.Conclusions: ASCs are not recruited as structural components of blood vessels but do appear to regulate endothelial progenitor-mediated angiogenesis. The results obtained show that ASC paracrine signaling promotes the formation of blood vessels by EPCs. ASC paracrine signaling appears to promote angiogenesis by increasing the migration and invasion of EPCs and simultaneously upregulating the expression of vascular endothelial markers in EPCs. The results of in vivo experiments showed that ASCs promote the formation of blood vessels in EPC cultures.

scholarly journals In Vitro Evaluation of Endothelial Progenitor Cells from Adipose Tissue as Potential Angiogenic Cell Sources for Bladder Angiogenesis

PLoS ONE ◽  
2015 ◽  
Vol 10 (2) ◽  
pp. e0117644 ◽  
Author(s):  
Liuhua Zhou ◽  
Jiadong Xia ◽  
Xuefeng Qiu ◽  
Pengji Wang ◽  
Ruipeng Jia ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Endothelial Progenitor ◽  
In Vitro Evaluation ◽  
Cell Sources

scholarly journals Combined Transplantation of Adipose Tissue-Derived Stem Cells and Endothelial Progenitor Cells Improve Diabetic Erectile Dysfunction in a Rat Model

2020 ◽  
Vol 2020 ◽  
pp. 1-15
Author(s):  
Qiyun Yang ◽  
Wanmei Chen ◽  
Chi Zhang ◽  
Yun Xie ◽  
Yong Gao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Erectile Dysfunction ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Rat Model ◽  
Erectile Function ◽  
Normal Group ◽  
Endothelial Progenitor ◽  
Intracavernous Injection

Erectile dysfunction (ED) is a common complication in men suffered with diabetic mellitus. Stem cell transplantation is a promising strategy for the treatment of diabetic ED (DED). In this study, we evaluated whether combined transplantation of adipose tissue-derived stem cells (ADSCs) and endothelial progenitor cells (EPCs) could improve the erectile function of the DED rat model. DED rats were induced via intraperitoneal injection of streptozotocin (50 mg/kg), and ED was screened by apomorphine (100 mg/kg). DED rats were divided into 4 groups (n=14 each): DED, ADSC, EPC, and ADSC/EPC group. Another 14 age-matched male SD rats with normal erectile function were served as the normal group. The normal group and the DED group were received intracavernous injection with phosphate-buffered saline (PBS). And the other groups were received intracavernous injection with ADSCs (1×106), EPCs (1×106), and ADSCs/EPCs (0.5×106/0.5×106), respectively. The total intracavernous pressure (ICP) and mean arterial pressure (MAP) were recorded at day 28 after injection. The endothelium, smooth muscle, and penile dorsal nerves were assessed within cavernoursal tissue. On day 28 after injection, the ADSC/EPC group displayed more significantly enhanced ICP and ICP/MAP than the DED or ADSC or EPC group (p<0.05). Immunofluorescent analysis and western blot demonstrated that the improvement of erectile function in the ADSC/EPC5 group was associated with increased expression of endothelial marker (CD31) and the correction of eNOS-cGMP-NO signaling. More 5-ethynyl-2′-deoxyuridine- (EdU-) positive EPCs could be found lining in the cavernous endothelial layer in the ADSC/EPC group than the EPC group, which was attributed to the paracrine of vascular endothelial growth factor (VEGF) and stromal-derived factor-1 (SDF-1) by ADSCs. Combined transplantation of ADSCs and EPCs has a synergic effect in repairing the endothelial function of DED rats, and the underlying mechanism might be the paracrine of VEGF and SDF-1 by ADSCs, which improves the recruitment and proliferation of EPCs in the cavernosum.

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