All-trans Retinoic Acid Improves Structure and Function of Diabetic Rat Skin in Organ Culture

Airway hyperresponsiveness (AHR) is influenced by structural components of the bronchial wall, including the smooth muscle and connective tissue elements and the neuromuscular function. AHR is also influenced by parenchymally derived tethering forces on the bronchial wall, which maintain airway caliber by producing outward radial traction. Our previous work has shown that vitamin A-deficient (VAD) rats exhibit cholinergic hyperresponsiveness and a decrease in the expression and function of the muscarinic-2 receptors (M2R). We hypothesized that if decreases in radial traction from airway or parenchymal structures contributed to the VAD-related increase in AHR, then the radial traction would normalize more slowly than VAD-related alterations in neurotransmitter signaling. Rats remained vitamin A sufficient (VAS) or were rendered VAD and then maintained on the VAD diet in the presence or absence of supplementation with all- trans retinoic acid (RA). VAD was associated with an approximately twofold increase in respiratory resistance and elastance compared with VAS rats. Exposure to RA for 12 days but not 4 days restored resistance and elastance to control (VAS) levels. In VAD rats, AHR was accompanied by decreases in bronchial M2R gene expression and function, which were restored after 12 days of RA supplementation. Subepithelial bronchial elastic fibers were decreased by ∼50% in VAD rats and were significantly restored by RA. The increase in AHR that is associated with VAD is accompanied by decreases in M2R expression and function that can be restored by RA and a reduction in airway elastic fibers that can be partially restored by RA.

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All-trans retinoic acid mediates DUOX2 expression and function in respiratory tract epithelium

AJP Lung Cellular and Molecular Physiology ◽

10.1152/ajplung.00015.2010 ◽

2010 ◽

Vol 299 (2) ◽

pp. L215-L221 ◽

Cited By ~ 14

Author(s):

Angela Lee Linderholm ◽

June Onitsuka ◽

Changhong Xu ◽

Maggie Chiu ◽

Wai-Ming Lee ◽

...

Keyword(s):

Retinoic Acid ◽

Epithelial Cells ◽

Respiratory Tract ◽

Fold Increase ◽

Respiratory Epithelial Cells ◽

Trans Retinoic Acid ◽

All Trans Retinoic Acid ◽

Mrna And Protein Expression ◽

And Function ◽

Respiratory Epithelial

DUOX1 and DUOX2 are members of the NADPH oxidase family that are specifically regulated to produce hydrogen peroxide in epithelia of the thyroid, gastrointestinal tract, and respiratory tract. The determinants of DUOX1 or DUOX2 expression in various tissues have not been established. Using respiratory tract epithelial cells as a model, we investigated changes in DUOX mRNA and protein expression during the first 10 days of differentiation. By comparing a respiratory tract cell line, HBE1, with primary tracheobronchial epithelial (TBE) cells, we determined that DUOX2 was significantly expressed only in cell conditions that included all- trans retinoic acid (ATRA). In HBE1 cells, DUOX2 mRNA increased 6-fold after ATRA treatment. Similarly, ATRA induced a 19-fold increase in DUOX2 mRNA expression in primary TBE cells with parallel increases in DUOX protein and DUOX-mediated H2O2 production as well. In addition, DUOX2 induction by rhinovirus required the presence of ATRA. ATRA had no effect on DUOX1 expression for all the conditions studied. Our data indicate that for respiratory epithelial cells, ATRA is important in the regulation of DUOX2 expression, function, and rhinovirus-mediated DUOX2 inducibility.

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All-trans retinoic acid improves the viability of ischemic skin flaps in diabetic rat models

Diabetes Research and Clinical Practice ◽

10.1016/j.diabres.2018.06.019 ◽

2018 ◽

Vol 142 ◽

pp. 385-392 ◽

Cited By ~ 2

Author(s):

Zhenjun Weng ◽

Chunyang Wang ◽

Cheng Zhang ◽

Jia Xu ◽

Yimin Chai ◽

...

Keyword(s):

Retinoic Acid ◽

Diabetic Rat ◽

Skin Flaps ◽

Rat Models ◽

Trans Retinoic Acid ◽

All Trans Retinoic Acid

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all-trans-Retinoic acid preserves viability of fibroblasts and keratinocytes in full-thickness human skin and fibroblasts in isolated dermis in organ culture

Archives of Dermatological Research ◽

10.1007/bf00371569 ◽

1994 ◽

Vol 286 (8) ◽

pp. 443-447 ◽

Cited By ~ 7

Author(s):

James Varani ◽

Patricia Perone ◽

Suzanne E. G. Fligiel ◽

Dennis R. Inman ◽

John J. Voorhees

Keyword(s):

Retinoic Acid ◽

Organ Culture ◽

Human Skin ◽

Full Thickness ◽

Trans Retinoic Acid ◽

All Trans Retinoic Acid

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Alterations of Intercellular Junctions in Peritoneal Mesothelial Cells from Patients Undergoing Dialysis: Effect of Retinoic Acid

Peritoneal Dialysis International ◽

10.3747/pdi.2012.00323 ◽

2015 ◽

Vol 35 (3) ◽

pp. 275-287 ◽

Cited By ~ 12

Author(s):

Carmen Retana ◽

Elsa Sanchez ◽

Alejandro Perez-Lopez ◽

Armando Cruz ◽

Jesus Lagunas ◽

...

Keyword(s):

Retinoic Acid ◽

Intercellular Junctions ◽

Mesothelial Cells ◽

Peritoneal Mesothelial Cells ◽

Solute Permeability ◽

Trans Retinoic Acid ◽

All Trans Retinoic Acid ◽

Peritoneal Permeability ◽

And Function ◽

Cell Contour

BackgroundDialysis patients are classified according to their peritoneal permeability as low transporter (LT, low solute permeability) or high transporter (HT, high solute permeability). Tight junction (TJ) proteins are critical to maintain ions, molecules and water paracellular transport through peritoneum. Exposure to peritoneal dialysis solutions causes damage to TJ in human peritoneal mesothelial cells (HPMCs). We analyzed the quantity, distribution and function of TJ proteins: claudin-1, -2 and -8, ZO-1 and occludin, in HPMC cultures from LT and HT patients. Since all-trans retinoic acid (ATRA) might modify the expression of TJ proteins, we studied its effect on HPMCs.MethodsControl HPMCs were isolated from human omentum, while HT or LT cells were obtained from dialysis effluents. Cells were cultured in presence of ATRA 0, 50 or 100 nM. Transepithelial electrical resistance (TER) measurement, immunostaining and Western blot analyses were performed.ResultsHT exhibited lower TER than control and LT monolayers. Immunofluorescence for TJ was weak and discontinuous along the cell contour, in LT and HT. Furthermore, claudin- 1, occludin and ZO-1 expressions were decreased. In all groups, claudin-2 was localized at nuclei. We observed that ATRA improved TJ distribution and increased TJ expression in HT. This retinoid did not modify claudin-2 and -8 expressions. All-trans retinoic acid decreased TER in HT, but had no effect in LT.ConclusionsTight junctions were altered in HPMCs from dialyzed patients. The HT monolayer has lower TER than LT, which might be associated with the peritoneal permeability in these patients. ATRA might be a therapeutic alternative to maintain mesothelial integrity, since it improved TJ localization and expression.

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