Inter- and Intra-patient Variability Over Time of Lesional Skin Microbiota in Adult Patients with Atopic Dermatitis

The skin microbiota of atopic dermatitis (AD) patients is characterized by increased Staphylococcus aureus colonization, which exacerbates disease symptoms and has been linked to reduced bacterial diversity. Skin bacterial communities in AD patients have mostly been described at family and genus levels, while species-level characterization has been limited. In this study, we investigated the role of the bacteria belonging to the Staphylococcus genus using targeted sequencing of the tuf gene with genus-specific primers. We compared staphylococcal communities on lesional and non-lesional skin of AD patients, as well as AD patients with healthy controls, and determined the absolute abundance of bacteria present at each site. We observed that the staphylococcal community, bacterial alpha diversity, and bacterial densities were similar on lesional and non-lesional skin, whereas AD severity was associated with significant changes in staphylococcal composition. Increased S. aureus, Staphylococcus capitis, and Staphylococcus lugdunensis abundances were correlated with increased severity. Conversely, Staphylococcus hominis abundance was negatively correlated with severity. Furthermore, S. hominis relative abundance was reduced on AD skin compared to healthy skin. In conclusion, various staphylococcal species appear to be important for skin health.

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DNA Methylation Analysis of Lesional and Non-lesional Skin in Adult Patients with Atopic Dermatitis

Molekulyarnaya Meditsina (Molecular medicine) ◽

10.29296/24999490-2021-01-08 ◽

2021 ◽

Vol 19 (1) ◽

pp. 53-64

Author(s):

Olga Smolkina ◽

Elizaveta Bystritskaia ◽

Oksana Svitich ◽

Anastas Piruzyan ◽

Elena Denisova ◽

...

Keyword(s):

Dna Methylation ◽

Atopic Dermatitis ◽

Adult Patients ◽

Methylation Analysis ◽

Lesional Skin ◽

Dna Methylation Analysis

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Experience of combined topical therapy with methylprednisoloni aceponatis and «Bepanten Plus»® in treatment of adult patients with moderate and severe atopic dermatitis

Rossiiskii Allergologicheskii ZHurnal ◽

10.36691/rja410 ◽

2015 ◽

Vol 12 (6) ◽

pp. 80-83

Author(s):

O K Shtyrbul ◽

O A Erina ◽

E S Fedenko

Keyword(s):

Staphylococcus Aureus ◽

Atopic Dermatitis ◽

Combined Therapy ◽

Topical Therapy ◽

Adult Patients ◽

Severe Atopic Dermatitis ◽

Bacterial Isolation ◽

Treatment Results ◽

Lesional Skin ◽

Days Of Therapy

Background. To estimate efficiency of combined therapy with methylprednisolone aceponatis and «Bepanten Plus»® in adult patients with moderate and severe atopic dermatitis. Methods. We examined 32 adult patients, who were treated with TGCS methylprednisolone aceponatis and «Bepanten Plus»®. The efficiency of therapy was estimated with index SCORAD, IGA and subjective patients assessment. For 15 of 32 patients swabs for bacterial isolation to estimate Staphylococcus aureus growth were taken from lesional skin before therapy and on the 14 th or 20 th days of treatment. Results. The аverage value of index SCORAD decreased after 15 days of therapy, Me 41,7 to Me 23,3 a point (p

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Shifts in the Skin Microbiota after UVB Treatment in Adult Atopic Dermatitis

Dermatology ◽

10.1159/000515236 ◽

2021 ◽

pp. 1-12

Author(s):

Astrid Haaskjold Lossius ◽

Olav Sundnes ◽

Anna Cäcilia Ingham ◽

Sofie Marie Edslev ◽

Jørgen Vildershøj Bjørnholt ◽

...

Keyword(s):

Atopic Dermatitis ◽

Temporal Dynamics ◽

Skin Barrier ◽

16S Rrna Gene Sequencing ◽

Ultraviolet B ◽

Rrna Gene ◽

Skin Microbiota ◽

Lesional Skin ◽

Before And After ◽

Nose And Throat

Background: The pathophysiology in atopic dermatitis (AD) is not fully understood, but immune dysfunction, skin barrier defects, and alterations of the skin microbiota are thought to play important roles. AD skin is frequently colonized with Staphylococcus aureus (S. aureus) and microbial diversity on lesional skin (LS) is reduced compared to on healthy skin. Treatment with narrow-band ultraviolet B (nb-UVB) leads to clinical improvement of the eczema and reduced abundance of S. aureus. However, in-depth knowledge of the temporal dynamics of the skin microbiota in AD in response to nb-UVB treatment is lacking and could provide important clues to decipher whether the microbial changes are primary drivers of the disease, or secondary to the inflammatory process. Objectives: To map the temporal shifts in the microbiota of the skin, nose, and throat in adult AD patients after nb-UVB treatment. Methods: Skin swabs were taken from lesional AD skin (n = 16) before and after 3 treatments of nb-UVB, and after 6–8 weeks of full-body treatment. We also obtained samples from non-lesional skin (NLS) and from the nose and throat. All samples were characterized by 16S rRNA gene sequencing. Results: We observed shifts towards higher diversity in the microbiota of lesional AD skin after 6–8 weeks of treatment, while the microbiota of NLS and of the nose/throat remained unchanged. After only 3 treatments with nb-UVB, there were no significant changes in the microbiota. Conclusion: Nb-UVB induces changes in the skin microbiota towards higher diversity, but the microbiota of the nose and throat are not altered.

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Temporal Variation in Staphylococcus aureus Protein A Genotypes from Nose and Skin in Atopic Dermatitis Patients

Dermatology ◽

10.1159/000515235 ◽

2021 ◽

pp. 1-7

Author(s):

Minke M.F. van Mierlo ◽

Suzanne G.M.A. Pasmans ◽

Joan E.E. Totté ◽

Jill de Wit ◽

Bjorn L. Herpers ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Atopic Dermatitis ◽

Temporal Variation ◽

Disease Severity ◽

Severe Disease ◽

Protein A ◽

Lesional Skin ◽

Over Time ◽

Skin Samples

Background: Staphylococcus aureus colonization is associated with disease severity in patients with atopic dermatitis (AD). Objective: To investigate temporal variation in S. aureus protein A gene (spa)-types isolated from the nose and lesional skin and the correlation of spa-types with disease severity. Results: This study included 96 adult AD patients who were assessed at baseline (T0) and after a strict 2-week follow-up period (T1) in which treatment was standardized with a topical corticosteroid. Fifty-five different spa-types were detected in the nose and skin cultures. Seventy-three patients were colonized with S. aureus in the nasal cavity at both time points (persistent carriership), 59 of whom (81%) had identical spa-types over time. For skin samples, 42 (75%) of the 56 persistent skin carriers had identical spa-types over time. The same spa-type was carried in the nose and skin in 79 and 77% of the patients at T0 and T1, respectively. More severe disease was not associated with specific spa-types or with temporal variation in spa-type. Conclusion: S. aureus strains in AD are highly heterogeneous between patients. The majority of patients carry the same spa-type in the nose and skin without temporal variation, suggesting clonal colonization within individual patients. No predominant spa-type or temporal variation is associated with increased disease severity.

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