Application of 2D-DIGE in Identification of Differentially Expressed Proteins of Mycobacterium Bovis BCG in Nonculturable and Rpf Mediated Resuscitation Phase

Bone marrow-derived mesenchymal stem cells (BM-MSCs) display high tumor tropism and cause indirect effects through the cytokines they secrete. However, the effects of BM-MSCs on the biological behaviors of glioblastoma multiforme remain unclear. In this study, the conditioned medium from BM-MSCs significantly inhibited the proliferation of C6 cells ( P < 0.05 ) but promoted their migration and invasion ( P < 0.05 ). Two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) proteomic analysis revealed 17 proteins differentially expressed in C6 cells exposed to the BM-MSC-conditioned medium including five upregulated proteins and 12 downregulated proteins. Among these, six differentially expressed proteins (Calr, Set, Oat, Npm1, Ddah1, and Tardbp) were closely related to cell proliferation and differentiation, and nine proteins (Pdia6, Sphk1, Anxa4, Vim, Tuba1c, Actr1b, Actn4, Rap2c, and Tpm2) were associated with motility and the cytoskeleton, which may modulate the invasion and migration of tumor cells. Above all, by identifying the differentially expressed proteins using proteomics and bioinformatics analysis, BM-MSCs could be genetically modified to specifically express tumor-suppressive factors when BM-MSCs are to be used as tumor-selective targeting carriers in the future.

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2D DIGE identifies differentially expressed proteins in postmenopausal women with osteopenia and osteoporosis

Bone ◽

10.1016/j.bone.2012.02.431 ◽

2012 ◽

Vol 50 ◽

pp. S139

Author(s):

L. Corrigan⁎ ◽

J. Browne ◽

K. Fitzgerald ◽

G.C. Chan ◽

S. Kennelly ◽

...

Keyword(s):

Postmenopausal Women ◽

Differentially Expressed ◽

Differentially Expressed Proteins ◽

2D Dige

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Candidate prioritization for low-abundant differentially expressed proteins in 2D-DIGE datasets

BMC Bioinformatics ◽

10.1186/s12859-015-0455-x ◽

2015 ◽

Vol 16 (1) ◽

Cited By ~ 1

Author(s):

Umesh K Nandal ◽

Wytze J Vlietstra ◽

Carsten Byrman ◽

Rienk E Jeeninga ◽

Jeffrey H Ringrose ◽

...

Keyword(s):

Differentially Expressed ◽

Differentially Expressed Proteins ◽

2D Dige

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Identification of Differentially-Expressed Proteins between Early Submucosal Non-Invasive and Invasive Colorectal Cancer Using 2D-DIGE and Mass Spectrometry

International Journal of Immunopathology and Pharmacology ◽

10.1177/039463201102400404 ◽

2011 ◽

Vol 24 (4) ◽

pp. 849-859 ◽

Cited By ~ 25

Author(s):

J. Zhang ◽

M-Q. Song ◽

J-S. Zhu ◽

Z. Zhou ◽

Z-P. Xu ◽

...

Keyword(s):

Colorectal Cancer ◽

Mass Spectrometry ◽

Differentially Expressed ◽

Differentially Expressed Proteins ◽

2D Dige ◽

Non Invasive

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195 SPERMATOZOAL PROTEIN MARKERS FOR ANGUS BULL FERTILITY

Reproduction Fertility and Development ◽

10.1071/rdv23n1ab195 ◽

2011 ◽

Vol 23 (1) ◽

pp. 198

Author(s):

X. Wang ◽

A. Kaya ◽

E. Memili

Keyword(s):

Semen Quality ◽

Expression Profiles ◽

Male Gamete ◽

Differentially Expressed ◽

Low Fertility ◽

Differentially Expressed Proteins ◽

Protein Markers ◽

2D Dige ◽

Animal Reproduction ◽

Fundamental Biology

Fertility is one of the most economically important traits controlling animal reproduction. Despite its significant economic impact, there are no reliable markers to predict semen quality. The objective of this study was to identify spermatozoal proteins associated with bull fertility, the ability of the sperm to fertilize the oocyte and support embryonic development. To accomplish our objectives, we isolated total spermatozoal proteins from 4 Angus bulls with different fertility phenotypes. Next, differentially expressed proteins were determined using two-dimensional differential in-gel electrophoresis (2D-DIGE), followed by sequencing of the most differentially expressed proteins. Immunoblotting experiments were conducted to confirm expression of key proteins detected by 2D-DIGE. Our results from 2D-DIGE experiments showed approximately 2000 detectable spermatozoal proteins. Of these comprehensive lists of proteins, we identified 80 of the proteins with the highest differential expression in spermatozoa from high- and low-fertility bulls. Diverse sets of differentially expressed proteins known to play roles in sperm motility, metabolism, and cell morphology were identified. These proteins included outer dense fibre of sperm tails 2 and manganous superoxide, heat shock protein, tubulins, α-enolase, and acrosomal vesicle protein-1. Expression profiles of outer dense fibre of sperm tails 2 and manganous superoxide dismutase were confirmed using immunoblotting. The findings are significant because they help us understand the fundamental biology of the male gamete and early development. In addition, the identified proteins can be used as molecular markers to predict bull fertility, an economically important trait. Funded in part by the Mississippi Agricultural and Forestry Experiment Station, American Angus Association, and Alta Genetics, Inc.

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