CMOS Biosensor IC with 360-Sensing Elements Using 63-GHz LC-Oscillator and DEP for Label-Free Single-Cell Detection

A sheath-less PDMS microfluidic IFC device with a simple structure was constructed, with a good performance in single-cell detection. The electrical conductance and susceptance were used to differentiate the beads/cells.

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Multiplexed and single-cell detection of microRNA with plasmonic nanoparticle assemblies

Canadian Journal of Chemistry ◽

10.1139/cjc-2021-0023 ◽

2021 ◽

Author(s):

Gurbrinder Ghotra ◽

Nguyen Le ◽

Heyam Hayder ◽

Chun Peng ◽

Jennifer I. L. Chen

Keyword(s):

Nucleic Acid ◽

Single Cell ◽

Low Cost ◽

Single Cells ◽

Cell Detection ◽

Label Free ◽

Plasmonic Nanoparticle ◽

Nanoparticle Assemblies ◽

Single Cell Detection ◽

Target Binding

We present a label-free, low cost and miniatured biosensing platform based on the disassembly of core-satellite plasmonic nanoparticle assemblies. The rapid and selective detection of an exemplary nucleic acid biomarker, has-miRNA-210-3p, was achieved via the strand displacement nucleic acid reaction. Target binding leads to dehybridization of the DNA linkers and changes in the scattering properties of nanostructures as monitored by darkfield microscopy. We demonstrate the ability to detect microRNA expunged from single cells and the potential to multiplex discrete assemblies to enable diverse biological applicability. The work may help translate the applicability of microRNA as diagnostic biomarkers, quantitate their abundance in the microenvironment, and facilitate the study of their correlation or causation to other biomolecules at the single-cell level.

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Integrated Field’s metal microelectrodes based microfluidic impedance cytometry for cell-in-droplet quantification

10.1101/278069 ◽

2018 ◽

Author(s):

Jatin Panwar ◽

Rahul Roy

Keyword(s):

Single Cell ◽

Single Cell Analysis ◽

Single Cells ◽

Microfluidic Channel ◽

High Sensitivity ◽

Cell Detection ◽

Label Free ◽

Single Cell Detection ◽

Electrode Fabrication ◽

Impedance Cytometry

AbstractMicrofluidic impedance cytometry (MIC) provides a non-optical and label-free method for single cell detection and classification in microfluidics. However, the cleanroom intensive infrastructure required for MIC electrode fabrication limits its wide implementation in microfluidic analysis. To bypass the conventional metal (platinum) electrode fabrication protocol, we fabricated coplanar ‘in-contact’ Field’s metal (icFM) microelectrodes in multilayer elastomer devices with a single photolithography step. Our icFM microelectrodes displayed excellent and comparable performance to the platinum electrodes for detection of single erythrocytes with a lock-in amplifier based MIC setup. We further characterized it for water-in-oil droplets generated in a T-junction microfluidic channel and found high sensitivity and long-term operational stability of these electrodes. Finally, to facilitate droplet based single cell analysis, we demonstrate detection and quantification of single cells entrapped in aqueous droplets.

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