A sheath-less electric impedance micro-flow cytometry device for rapid label-free cell classification and viability testing

2017 ◽  
Vol 9 (7) ◽  
pp. 1201-1212 ◽  
Author(s):  
Xinwu Xie ◽  
Zhen Cheng ◽  
Youchun Xu ◽  
Ran Liu ◽  
Qi Li ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Single Cell ◽  
Simple Structure ◽  
Electrical Conductance ◽  
Free Cell ◽  
Cell Detection ◽  
Label Free ◽  
Electric Impedance ◽  
Single Cell Detection ◽  
Micro Flow

A sheath-less PDMS microfluidic IFC device with a simple structure was constructed, with a good performance in single-cell detection. The electrical conductance and susceptance were used to differentiate the beads/cells.

scholarly journals Multiplexed and single-cell detection of microRNA with plasmonic nanoparticle assemblies

2021 ◽  
Author(s):  
Gurbrinder Ghotra ◽  
Nguyen Le ◽  
Heyam Hayder ◽  
Chun Peng ◽  
Jennifer I. L. Chen
Keyword(s):  
Nucleic Acid ◽  
Single Cell ◽  
Low Cost ◽  
Single Cells ◽  
Cell Detection ◽  
Label Free ◽  
Plasmonic Nanoparticle ◽  
Nanoparticle Assemblies ◽  
Single Cell Detection ◽  
Target Binding

We present a label-free, low cost and miniatured biosensing platform based on the disassembly of core-satellite plasmonic nanoparticle assemblies. The rapid and selective detection of an exemplary nucleic acid biomarker, has-miRNA-210-3p, was achieved via the strand displacement nucleic acid reaction. Target binding leads to dehybridization of the DNA linkers and changes in the scattering properties of nanostructures as monitored by darkfield microscopy. We demonstrate the ability to detect microRNA expunged from single cells and the potential to multiplex discrete assemblies to enable diverse biological applicability. The work may help translate the applicability of microRNA as diagnostic biomarkers, quantitate their abundance in the microenvironment, and facilitate the study of their correlation or causation to other biomolecules at the single-cell level.

CMOS Biosensor IC with 360-Sensing Elements Using 63-GHz LC-Oscillator and DEP for Label-Free Single-Cell Detection

2018 ◽  
Author(s):  
Takeshi Mitsunaka ◽  
Masafumi Yamanoue ◽  
Kunihiko Iizuka ◽  
Soo Hyon Kim ◽  
Teruo Fujii ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Detection ◽  
Label Free ◽  
Single Cell Detection ◽  
Lc Oscillator

scholarly journals Quantitative single-cell detection of poly(β-hydroxybutyrate) accumulation inRhizobium melilotiby flow cytometry

1995 ◽  
Vol 126 (1) ◽  
pp. 31-35
Author(s):  
P Fouchet ◽  
S Jan ◽  
J Courtois ◽  
B Courtois ◽  
G Frelat ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Single Cell ◽  
Cell Detection ◽  
Single Cell Detection

Label-Free Density-Based Detection of Adipocytes of Bone Marrow Origin Using Magnetic Levitation

2018 ◽  
Author(s):  
Oyku Sarigil ◽  
Muge Anil-Inevi ◽  
Gulistan Mese ◽  
H. Cumhur Tekin ◽  
Engin Ozcivici
Keyword(s):  
Flow Cytometry ◽  
Magnetic Levitation ◽  
Adipogenic Differentiation ◽  
Cell Detection ◽  
Label Free ◽  
Flow Process ◽  
Technical Developments ◽  
Single Cell Detection ◽  
Bone Marrow Origin ◽  
Microscopy Techniques

ABSTRACTAdipocyte hypertrophy and hyperplasia are important parameters in describing abnormalities in adipogenesis that are concomitant to diseases such as obesity, diabetes, anorexia nervosa and osteoporosis. Therefore, technical developments in detection of adipocytes become an important driving factor in adipogenesis research. Current techniques such as optical microscopy and flow cytometry are available in detection and examination of adipocytes, driving cell- and molecular-based research of adipogenesis. Even though microscopy techniques are common and straightforward, they are restricted for manipulation and separation of the cells. Flow cytometry is an alternative, but mature adipocytes are fragile and cannot withstand the flow process. Other separation methods usually require labeling of the cells or usage of microfluidic platforms that utilize fluids with different densities. Magnetic levitation is a novel label-free technology with the principle of movement of cells towards the lower magnetic field in a paramagnetic medium depending on their individual densities. In this study, we used magnetic levitation device for density-based single cell detection of differentiated adipogenic cells in heterogeneous populations. Results showed that magnetic levitation platform was sensitive to changes in lipid content of mesenchymal stem cells committed to adipogenesis and it could be successfully used to detect adipogenic differentiation of cells.

scholarly journals Integrated Field’s metal microelectrodes based microfluidic impedance cytometry for cell-in-droplet quantification

2018 ◽  
Author(s):  
Jatin Panwar ◽  
Rahul Roy
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Single Cells ◽  
Microfluidic Channel ◽  
High Sensitivity ◽  
Cell Detection ◽  
Label Free ◽  
Single Cell Detection ◽  
Electrode Fabrication ◽  
Impedance Cytometry

AbstractMicrofluidic impedance cytometry (MIC) provides a non-optical and label-free method for single cell detection and classification in microfluidics. However, the cleanroom intensive infrastructure required for MIC electrode fabrication limits its wide implementation in microfluidic analysis. To bypass the conventional metal (platinum) electrode fabrication protocol, we fabricated coplanar ‘in-contact’ Field’s metal (icFM) microelectrodes in multilayer elastomer devices with a single photolithography step. Our icFM microelectrodes displayed excellent and comparable performance to the platinum electrodes for detection of single erythrocytes with a lock-in amplifier based MIC setup. We further characterized it for water-in-oil droplets generated in a T-junction microfluidic channel and found high sensitivity and long-term operational stability of these electrodes. Finally, to facilitate droplet based single cell analysis, we demonstrate detection and quantification of single cells entrapped in aqueous droplets.

Single-cell detection and classification in phase contrast images

2010 ◽  
Author(s):  
M. Athelogou ◽  
M. Eblenkamp ◽  
G. Schmidt ◽  
F. Novotny ◽  
E. Wintermantel ◽  
...  
Keyword(s):  
Single Cell ◽  
Phase Contrast ◽  
Cell Detection ◽  
Single Cell Detection

scholarly journals Fluorescence enhanced BA-LIFT for single cell detection and isolation

2020 ◽  
Vol 12 (2) ◽  
pp. 025019 ◽  
Author(s):  
A Marquez ◽  
M Gómez-Fontela ◽  
S Lauzurica ◽  
R Candorcio-Simón ◽  
D Munoz-Martin ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Detection ◽  
Single Cell Detection

Single-Cell Detection of Trans-Splicing Ribozyme In Vivo Activity

2004 ◽  
Vol 126 (23) ◽  
pp. 7158-7159 ◽  
Author(s):  
Sumitaka Hasegawa ◽  
Jung W. Choi ◽  
Jianghong Rao
Keyword(s):  
Single Cell ◽  
Cell Detection ◽  
Single Cell Detection ◽  
Trans Splicing
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