scholarly journals CHARACTERISTICS AND TOXICITY OF NANOEMULSION FORMULATION OF PIPER RETROFRACTUM AND TAGETES ERECTA EXTRACT MIXTURES

2018 ◽  
Vol 18 (1) ◽  
pp. 1 ◽  
Author(s):  
Ni Siluh Putu Nuryanti ◽  
Edhi Martono ◽  
Endang Sri Ratna ◽  
Dadang .

Characteristics and Toxicity of Nanoemulsion Formulations of Piper retrofractum and Tagetes erecta Extract Mixtures. Nanotechnology has been used in the developing of botanical insecticide formulation for improving its stability and effectiveness. The research was aimed to make nanoemulsion formulations of Piper retrofractum fruits and Tagetes erecta flowers extracts and to evaluate their toxicity against brown planthopper (Nilaparvata lugens [Stål]) nymphs. The development of nanoemulsion formulaions was carried out by using the low energy method with inversion phase emulsification.  The toxicity of the four formulations was tested against brown planthopper nymphs using a contact method. Four nanoemulsion formulations were obtained.  The study showed that in the developing of the formulations, the type and level of emulsifier materials affected the physical characteristics of formulas, such as stability, surface tension, viscosity, particle size, and particle morphology. The nanoemulsion formulation containing 1% of the mixture of P. retrofractum fruits and T. erecta flowers extracts, 1.75% Triton X-100 emulsifier showed the highest toxic on the brown planthopper nymph, with the LC95 value was 0.15%.  The study indicates that nanoemulsion formulation of P. retrofractum and T. erecta extracts are potential to be developed as botanical insecticide to control brown planthopper of rice.

2020 ◽  
Vol 3 (2) ◽  
pp. 42
Author(s):  
Martua Suhunan Sianipar ◽  
Tarkus Suganda ◽  
Azhhar Hadyarrahman

The Brown Planthopper (Nilaparvata lugens Stal.) is one of the pests that often causes rice crop failure in rice. One way to control N. lugens pests is to use of botanical insecticide. The purpose of this experiment is to study the effect of A. cordifolia leaves ethanol in suppressing N. lugens populations. The experiment used a Randomized Block Design (RDB) with 8 treatments and 4 replications. The A. cordifolia leaves ethanol extract concentration tested in this experiment consisted of 0,5%; 0,75%; 1%; 1,5%; and 2%.  The results of the experiment showed that the ethanol extract of binahong leaves with a concentration of 1%, 1.5% and 2% cause mortality of N. lugens by 52.50%, 56.25%, and 61.25% on the 14th day after application. Based on the results of this experiment, the ethanol extract of A.cordifolia leaves was not effective against N. lugen.


Author(s):  
M S Noor Hasyierah ◽  
A Norhidayah ◽  
M Alina Rahayu ◽  
A Adilah ◽  
I Nur Humaira

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e47413 ◽  
Author(s):  
Jiranan Piyaphongkul ◽  
Jeremy Pritchard ◽  
Jeff Bale

2015 ◽  
Vol 105 (4) ◽  
pp. 390-398 ◽  
Author(s):  
P.-J. Wan ◽  
L. Yang ◽  
S.-Y. Yuan ◽  
Y.-H. Tang ◽  
Q. Fu ◽  
...  

AbstractThe brown planthopper Nilaparvata lugens is a serious phloem-feeding pest of rice in China. The current study focuses on a saccharopine dehydrogenase (SDH) that catalyzes the penultimate reaction in biosynthesis of the amino acid lysine (Lys), which plays a role in insect growth and carnitine production (as a substrate). The protein, provisionally designated as NlylsSDH [a SDH derived from yeast-like symbiont (YLS) in N. lugens], had a higher transcript level in abdomens, compared with heads, wings, legs and thoraces, which agrees with YLS distribution in N. lugens. Ingestion of Nlylssdh targeted double-stranded RNA (dsNlylssdh) for 5, 10 and 15 days decreased the mRNA abundance in the hoppers by 47, 70 and 31%, respectively, comparing with those ingesting normal or dsegfp diets. Nlylssdh knockdown slightly decreased the body weights, significantly delayed the development of females, and killed approximately 30% of the nymphs. Moreover, some surviving adults showed two apparent phenotypic defects: wing deformation and nymphal cuticles remained on tips of the legs and abdomens. The brachypterours/macropterours and sex ratios (female/male) of the adults on the dsRNA diet were lowered compared with the adults on diets without dsRNA. These results suggest that Nlylssdh encodes a functional SDH protein. The adverse effect of Nlylssdh knockdown on N. lugens implies the importance of Lys in hopper development. This study provides a proof of concept example that Nlylssdh could serve as a possible dsRNA-based pesticide for planthopper control.


Agrologia ◽  
2018 ◽  
Vol 7 (2) ◽  
Author(s):  
Martua Suhunan Sianipar

Brown Planthopper (Nilaparvata lugens Stal.) is a main pest of paddy. The purpose of this research is to study the population of Brown Planthopper and the natural enemy. The method of this research using survey. Survey was conducted in three experimental plots at JatisariSubdistrict, Karawang District, West Javaand in the greenhouse of the Department of Plant Pests and Deseases, Faculty of Agriculture,  Universitas Padjadjaran. The sample were collected by systematically diagonal using yellow stiky trap and manually or visual counting. The result showed population fluctuation BPH below the economy line where is temperature, humidity and rainfall did not significantly effect to the BPH population. It was indicated by the regression analysis of each temperature Y= (- 24.225 + 1.008 X1 ; R2 =0,020; P/Sign = 0.660),  Humidity  (Y=- 32.337 + 0.424 X2; R2  = 0,124; P/ Sign  = 0.261) and Rainfall (Y=1.707 + 0.090 X3 ; R2  = 0.050; P/ Sign = 0.487). There is a relation between temperature, humidity and rainfall to BPH (Y = - 123 + 2.685 T + 0.649 RH - 0.14 CH; R2 = 0.236; P/ Sign = 0.516) dit not significantly because as the value of significance (P = Sign)  > 0.05.8. The diversity index was moderate 1.88


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