scholarly journals KERAPATAN DAN VIABILITAS KONIDIA BEAUVERIA BASSIANA DAN METARHIZIUM ANISOPLIAE PADA MEDIA IN VITRO PH RENDAH

2017 ◽  
Vol 17 (2) ◽  
pp. 119
Author(s):  
Lilian Rizkie ◽  
Siti Herlinda ◽  
Suwandi . ◽  
Chandra Irsan ◽  
Susilawati . ◽  
...  
Keyword(s):  
Shelf Life ◽  
Beauveria Bassiana ◽  
Active Ingredient ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Low Ph ◽  
Conidial Viability

Conidial density and viability of Beauveria bassiana and Metarhizium anisopliae grown on the low-pH in vitro medium. Liquid bioinsecticide with active ingredient from conidial entomopathogenic fungus has major constraints, namely short shelf life due to declining conidial viability and density is caused by low pH in the bioinsecticide carrier. This experiment aimed to measure the loss of conidial viability and density of Beauveria bassiana and Metarhizium anisopliae isolates grown on in vitro medium with low pH. Entomopathogenic fungus isolates were used as much as 28 isolates grown on in vitro medium at low pH, namely pH 5, 4, 3, and 2. The results showed that the fungus isolate that had the highest conidial density on in vitro medium at pH 5 was found on isolates of B. bassiana with code BPcMs (2.583 x 109 conidia mL-1), while the lowest one was found on isolates of B. bassiana with code of BWS Pantura (0.825x109 conidia mL-1). All isolate conidial density from in vitro medium with pH 2 decreased regularly. Conidial density of BPcMs isolate decreased to 2.483 x 109conidia mL-1, as well as BWS Pantura isolate also decreased to 0.425x109 conidia mL-1. The highest conidial viability at pH 5 was found on isolates of B. bassiana with code of BPcMs (51.572%), while the lowest conidial viability was found on isolate of B. bassiana with BTmPc code (15.040%). At pH 2, almost isolates tested had low conidial viability. The conidial viability of isolates BPcMs decreased to 47.037%%, while the isolates BTmPc also decreased to 12.778%. Therefore, the lower of the pH of the in vitro medium was, the lower of conidial viability and density of B. bassiana and M. anisopliae was

scholarly journals UJI EFIKASI BIOINSEKTISIDA JAMUR ENTOMOPATOGEN BERFORMULASI CAIR TERHADAP Plutella xylostella (L.) DI LABORATORIUM

2013 ◽  
Vol 13 (1) ◽  
pp. 52-60
Author(s):  
Haperidah Nunilahwati ◽  
Siti Herlinda ◽  
Chandra Irsan ◽  
Yulia Pujiastuti ◽  
Khodijah Khodijah ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Active Ingredient ◽  
Plutella Xylostella ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Crop Production ◽  
Insect Pest ◽  
Larval Mortality ◽  
Third Instar Larvae

Efficacy test of liquid bio-insecticide of entomopathogenic fungi in control against Plutella xylostella in the laboratory.  The insect pest P. xylostella could reduce crop production of Brassicaceae. The aim of research was to test the efficacy liquid bio insecticide with active ingredient of Beauveria bassiana and Metarhizium anisopliae fungi to control P. xylostella. Bio-insecticide was applied by spraying  on mustard leaves infested with 50 individuals of third instar larvae of P. xylostella and a density of 1x106 conidia ml-1. Larval mortality was observed every 2 hours and LT50 of larvae was calculated. The study showed that the highest percentage of mortality found in Mt ES and Mt ES (cf) isolates was 99.6%, the lowest mortality at Mt NES isolate was 96.80%. LT50 and LT95 values   Bb ES were the lowest i.e. 2.04 days and 2.95 days. The highest LT50 and LT95 of Mt NES isolate were 2.24 days and 3.32 days. The liquid bio-insecticide of entomopathogenic fungus B. bassiana and M. anisopliae were effective to control the larvae of P. xylostella.

scholarly journals Effect of Biofertilizers and Neem Oil on the Entomopathogenic Fungi Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metsch.) Sorok

2001 ◽  
Vol 44 (4) ◽  
pp. 419-423 ◽  
Author(s):  
Edson Hirose ◽  
Pedro M. O. J. Neves ◽  
João A. C. Zequi ◽  
Luís H. Martins ◽  
Cristiane H. Peralta ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Azadirachta Indica ◽  
Organic Agriculture ◽  
Metarhizium Anisopliae ◽  
Neem Oil ◽  
Colony Diameter ◽  
Negative Effect ◽  
Fungitoxic Effect

The in vitro fungitoxic effect of three biofertilizers, E.M.-4, Multibion <FONT FACE=Symbol>Ô</FONT> and Supermagro used in organic agriculture and the neem oil (Azadirachta indica A. Juss) on the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana was studied. These products were mixed in a medium where the two fungi were inoculated, and germination, vegetative growth and conidiogenesis were assessed. The biofertilizers Supermagro and E.M.- 4 showed to be less toxic for the two fungi whereas Multibion<FONT FACE=Symbol>Ô</FONT> caused major inhibition on M. anisopliae, with reductions in germination (-37.74%), colony diameter (-30.26%) and conidiogenesis (-42.62%). Neem oil promoted a larger negative effect on B. bassiana, inhibiting germination (-45.27%), colony diameter (-36.62%) and conidiogenesis (-84.93%).

scholarly journals Compatibility between the entomopathogenic fungus Beauveria bassiana and insecticides used in coffee plantations

2003 ◽  
Vol 60 (4) ◽  
pp. 663-667 ◽  
Author(s):  
Carolina Natali de Oliveira ◽  
Pedro Manuel Oliveira Janeiro Neves ◽  
Lídio Sueki Kawazoe
Keyword(s):  
Beauveria Bassiana ◽  
Vegetative Growth ◽  
Entomopathogenic Fungus ◽  
Microbial Control ◽  
Infection Process ◽  
Conservation Strategy ◽  
Coffee Plantations ◽  
Conidia Germination ◽  
Fungus Beauveria Bassiana

Microbial control in integrated pest management (IPM) programs of coffee plantations is an important factor for the reduction of pest population densities. The use of selective pesticides can be associated with entomopathogens, increasing the efficiency of the control and reducing the use of required insecticides. The in vitro fungitoxic effect of insecticide formulations of Thiamethoxam, Cyfluthrin, Deltamethrin, Alpha-Cypermethrin, Triazophos, Chlorpyrifos, Fenpropathrin and Endosulfan and Beauveria bassiana (CG 425 strain) was evaluated at three concentrations (FR = average field recommendation; 0.5 ´ FR and 2 ´ FR). Effects of these products on conidia germination, vegetative growth and sporulation were compared. Only five insecticides, at the FR concentration, promoted conidia viability higher than 60%. Viability should be considered the most important factor to be evaluated since it is the first step of the infection process. The insecticide formulations of Alpha-Cypermethrin, Thiamethoxam and Cyfluthrin caused the lower inhibition level on conidia germination at the two lower concentrations, with no difference in relation to the control. With respect to vegetative growth analysis, Thiamethoxam at the two lower concentrations was not found to cause radial growth inhibition. Thiamethoxam caused the smallest inhibition level with regard to conidia production. The use of Alpha-Cypermethrin and Thiamethoxam formulations in coffee IPM programs for a B. bassiana inoculum conservation strategy are recommended, since these products were compatible with the entomopathogenic fungus Beauveria bassiana (CG 425), an important natural control agent of the coffee berry borer, Hypothenemus hampei.

scholarly journals Effect of Agrochemicals Used in the Cultivation of Soybean and Irrigated Rice on Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metsch.) Sorok

2019 ◽  
Vol 11 (17) ◽  
pp. 167
Author(s):  
Vanessa P. Gonçalves ◽  
Cândida R. J. de Farias ◽  
Victoria Moreira-Nunêz ◽  
Renata Moccellin ◽  
Viviana Gaviria-Hernández ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Spore Germination ◽  
Metarhizium Anisopliae ◽  
Mycelial Growth ◽  
Potato Dextrose Agar ◽  
Irrigated Rice ◽  
Spray Method ◽  
Fungitoxic Effect

The objective of this work was to evaluate the fungitoxic effect of the agrochemicals used in the cultivation of soybean and irrigated rice on entomopathogenic fungi such as Beauveria bassiana and Metarhizium anisopliae by means of the mycelial growth, sporulation and spore germination. The isolates were inoculated in potato-dextrose-agar (PDA) medium containing the pesticides and exposed to spraying with the products. It was observed that chlorantraniliprole produced the best results regarding and was compatible with the two methods of contact of the product with the fungi. Flubendiamide it&rsquo;s very toxic to fungi, producing 100% inhibition when incorporated into the medium, when by spraying, the fungus M. anisopliae got mycelial growth. Etofenprox and thiamethoxam changed their toxity classification according to the method of contact with product, was moderately compatible to B. bassiana and compatible to M. anisopliae and spinosad showed more compatibility with M. anisopliae than B. bassiana and was classified as compatible. Among fungicides the tricyclazole was the only compatible with the fungus by the spray method. The results showed that in vitro agrochemicals such as tricyclazole, thiamethoxam, flubendiamide and etofenprox are harmful to fungi. It was found that environmental interference could minimize the effects on organisms, especially when the chemicals are applied by spraying.

scholarly journals Entomopathogenic fungi isolated in the vicinity of Szczecin

2014 ◽  
Vol 33 (1) ◽  
pp. 123-130 ◽  
Author(s):  
Ryszard Miętkiewicz ◽  
Magdalena Dzięgielewska ◽  
Krystyna Janowicz
Keyword(s):  
Forest Soil ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Dominant Species ◽  
Beech Forest ◽  
Meadow Soil ◽  
Paecilomyces Farinosus ◽  
Opposite Tendency

The spectrum and occurrence of entomopathogenic fungi in litter beech forest. meadow soil and arable soil were evaluated using an "insect bait method". Soil and litter samples taken in autumn and spring from each stand were baited with <i>G. mellonella</i> larvae at 22°C. Six species of entomopathogenic fungi infected these larvae. In the litter <i>Beauveria bassiana</i> was the dominant species. Besides <i>B. bassiana</i>, 23% of <i>G. mellonella</i> larvae were infected by <i>Paecilomyces farinosus</i> in autumn. In the forest soil the dominant entomopathogenic fungus was not found. In this stand the number of infected larvae was the lowest. <i>Metarhizium anisopliae</i> was the dominant species in the meadow soil in both sampling periods. In the soil taken from a rye field<i> P. fumosoroseus</i> and <i>B. bassiana</i> infected <i>G. mellonella</i> larvae almost to the same degree. It was established that <i>B. bassiana</i> and <i>M. anisopliae</i> infected more <i>G. mellonella</i> larvae in spring than in autumn. In the case of <i>P. farinosus</i> and <i>P. fumosoroseus</i> an opposite tendency was observed.

scholarly journals PATOGENISITAS ISOLAT BEAUVERIA BASSIANA DAN Metarhizium anisopliae ASAL TANAH LEBAK DAN PASANG SURUT SUMATERA SELATAN UNTUK AGENS HAYATI Scirpophaga incertulas

2013 ◽  
Vol 13 (1) ◽  
pp. 10-18
Author(s):  
Rosdah Thalib ◽  
Redi Fernando ◽  
Khodijah Khodijah ◽  
Dewi Meidalima ◽  
Siti Herlinda
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Tenebrio Molitor ◽  
Biological Agents ◽  
Lowland Rice ◽  
Scirpophaga Incertulas ◽  
Conidial Viability

Pathogeicity of Beauveria bassiana and  Metarhizium anisopliae Isolates from Fresh Swamp and  Tidal Lowland, South Sumatra for Scirpophaga incertulas Biological Agents.  The objectives of the research weret o explore and to determine the pathogenicity of entomopathogenic  fungi againts the larvae of Scirpophaga incertulas, and to measure conidial viability and density of the fungi.  The method for fungi exploration used larvae of Tenebrio molitor baiting submerged in the soil.  The soil was taken from fresh swampand tidal lowland rice in South  Sumatra.  From the exploration study, we found two species of entomopathogenic fungi: Beauveria bassiana and Metarhizium anisopliae. Mortality of S. incertulas larvae that had been treated topically with fungal conidia (1x106 conidia ml-1) varied among the isolates. The highest mortality (98.33%) caused by BPlus isolate of B. Bassiana and the lowest by MtmIn  isolate of M. anisopliae (57.50%) and BTmTr  isolate of B. bassiana (57.50%).  The fungal colonies grew fast from the second day up to the fourth day after incubation but the growth became slow after the fifth day.  The highest conidial density was resulted by   BPcMs of B. bassiana isolate (63.33x106 conidia ml-1) but  this density was not significantly different from that of the BPlus  of B. bassiana isolate (63.11x106 conidia ml-1).  The lowest conidial density found in BTmTr of B. bassiana isolate (20.97x106 conidia ml-1) .   The isolate B. bassiana was more effective than M. anisopliae againt the larvae of S.incertulas.

scholarly journals Entomopathogens of Anαcridium αegyptium L. in Crete

2017 ◽  
Vol 14 ◽  
pp. 5
Author(s):  
Ν. Ε. Roditakis ◽  
D. Kollaros ◽  
Α. Legakis
Keyword(s):  
Beauveria Bassiana ◽  
Comparative Studies ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Serial Dilution ◽  
Local Isolate ◽  
Conidial Concentration ◽  
First Time ◽  
Fungus Beauveria Bassiana ◽  
Metarhizium Anisopliae Var Acridum

The entomopathogenic fungus Beauveria bassiana (Bals.) Vuil. was recorded for the first time on Anacridium aegyptium L. in Crete. The insects were fed on pieces of leaf subjected to a serial dilution of spores over three to four orders of magnitute. Comparative studies on the virulence of B. bassiana (I 91612 local isolate) and Metarhizium anisopliae var. acridum (IMI 330189 standard isolate of IIBC) showed that M. anisopliae var. acridum was more virulent than B. bassiana at a conidial concentration lower or equal to 106 per ml while they were similarly virulent on first stage nymphs at 107 conidia per ml.

scholarly journals Ação in vitro dos fungos Beauveria bassiana (Bals) Vuill e Metarhizium anisopliae (Metsch) Sorok sobre ninfas e adultos de Amblyomma cajennense (Fabricius, 1787) (Acari: Ixodidae)

2001 ◽  
Vol 53 (5) ◽  
pp. 544-547 ◽  
Author(s):  
R.C.S. Reis ◽  
D.R. Melo ◽  
E.J. Souza ◽  
V.R.E.P. Bittencourt
Keyword(s):  
Beauveria Bassiana ◽  
Metarhizium Anisopliae ◽  
Amblyomma Cajennense

O trabalho teve por objetivo verificar a mortalidade in vitro de ninfas e adultos de Amblyomma cajennense frente à ação de isolados dos fungos Beauveria bassiana e Metarhizium anisopliae. Foram avaliados três isolados de M. anisopliae (959, 319 e E9) e dois de B. bassiana (986 e 747). As suspensões de conídios foram preparadas a partir de fungos produzidos em meio de arroz, e cada bioensaio foi constituído de quatro tratamentos nas concentrações 10(5), 10(6), 10(7), 10(8) conídios/ml e um grupo-controle. A análise constou da observação do percentual de mortalidade, 15 dias após o tratamento ou após a ecdise de adultos. Foram observadas diferenças significativas entre os tratamentos quanto à mortalidade para todos os isolados e todos os instares. Nos grupos tratados houve aumento considerável na mortalidade à medida que se aumentava a concentração de conídios na suspensão. Conclui-se que todos os isolados testados causaram mortalidade em testes in vitro sobre esses estádios evolutivos, sugerindo o controle do A. cajennense pela ação desses fungos.

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