scholarly journals Determination of Dynamic Plasma Thiol -disulfide Homeostasis with a Novel Technique in Intestinal Ischemia Reperfusion Injury

2021 ◽  
Vol 2 (3) ◽  
pp. 55-59
Author(s):  
Ersin Gürkan Dumlu ◽  
Mehmet Tokaç ◽  
Mustafa Özsoy ◽  
İbrahim Kılınç ◽  
Nazmiye Dinçer ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Intestinal Ischemia ◽  
Sham Group ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Albino Rats ◽  
Sham Operation ◽  
Total Thiol ◽  
Ischemia Group ◽  
Intestinal Ischemia Reperfusion

Introduction: Intestinal ischemia/reperfusion (I/R) is a serious life-threatening clinical case. Overproduction of reactive oxygen species (ROS) associated with oxidative stress plays a key role in I/R pathophysiology. Objective: The aim of this study is to evaluate dynamic thiol / disulfide hemostasis in intestinal I/R in rats. Materials and Methods: 24 Winstar albino rats were divided into 3 groups (8 animals in each group: (1) sham (operation without ischemia), (2) ischemia (90 minutes ischemia), (3) ischemia/reperfusion (after ischemia for 30 minutes reperfusion for 60 minutes). At the end of the process, liver samples were evaluated pathologically. Also, in plasma samples, native thiol, disulphide and total thiol levels were measured. Results: Native thiol levels in ischemia and I/R group were found to be significantly lower compared to the sham group (P<0.001). Disulfide levels in Ischemia group were found to be significantly higher compared to the sham group (P< 0.05). Disulfide / native thiol levels in ischemia and I/R group were found to be significantly higher compared to the sham group (P < 0.05). Disulfide / total thiol levels in both ischemia and I/R group were found to be significantly higher compared to the sham group (P< 0.05). Conclusion: Balance of dynamic thiol/disulfide is a quite suitable indicator for evaluating oxidative stress which occurs as a result of intestinal I/R. As a result, measurement of dynamic thiol/disulfide balance with Erel method is fully automated and practical for evaluating oxidative environment which is formed with ischemia reperfusion.

scholarly journals Ischemic Postconditioning Alleviates Intestinal Ischemia-Reperfusion Injury by Enhancing Autophagy and Suppressing Oxidative Stress through the Akt/GSK-3β/Nrf2 Pathway in Mice

2020 ◽  
Vol 2020 ◽  
pp. 1-14 ◽  
Author(s):  
Rong Chen ◽  
Yun-yan Zhang ◽  
Jia-nan Lan ◽  
Hui-min Liu ◽  
Wei Li ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Intestinal Ischemia ◽  
Nuclear Translocation ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Ischemic Postconditioning ◽  
Intestinal Barrier Function ◽  
Protective Effects ◽  
Nrf2 Pathway ◽  
Intestinal Ischemia Reperfusion

Aims. Ischemic postconditioning (IPO) has a strong protective effect against intestinal ischemia-reperfusion (IIR) injury that is partly related to autophagy. However, the precise mechanisms involved are unknown. Methods. C57BL/6J mice were subjected to unilateral IIR with or without IPO. After 45 min ischemia and 120 min reperfusion, intestinal tissues and blood were collected for examination. HE staining and Chiu’s score were used to evaluate pathologic injury. We test markers of intestinal barrier function and oxidative stress. Finally, we used WB to detect the expression of key proteins of autophagy and the Akt/GSK-3β/Nrf2 pathway. Results. IPO significantly attenuated IIR injury. Expression levels of LC3 II/I, Beclin-1, and p62 were altered during IIR, indicating that IPO enhanced autophagy. IPO also activated Akt, inhibited GSK-3β, induced Nrf2 nuclear translocation, and upregulated HO-1 and NQO1 expression, thus providing protective effects against IIR injury by suppressing oxidative stress. Consistently, the beneficial effects of IPO were abolished by pretreatment with 3-methyladenine, SC66, and brusatol, potent inhibitors of autophagy, Akt, and Nrf2, respectively. Conclusion. Our study indicates that IPO can ameliorate IIR injury by evoking autophagy, activating Akt, inactivating GSK-3β, and activating Nrf2. These findings may provide novel insights for the alleviation of IIR injury.

The protective effects of urapidil on lung tissue after intestinal ischemia-reperfusion injury

2019 ◽  
Vol 44 (4) ◽  
pp. 539-548 ◽  
Author(s):  
Deniz Ozturk ◽  
Derya Guzel Erdogan ◽  
Ayhan Tanyeli ◽  
Selim Çomaklı ◽  
Hüseyin Baylan ◽  
...  
Keyword(s):  
Lung Tissue ◽  
Intestinal Ischemia ◽  
Caspase 3 ◽  
Sham Group ◽  
Ischemia Reperfusion ◽  
Sham Operation ◽  
Group 4 ◽  
Antioxidant Agents ◽  
Group 2 ◽  
Intestinal Ischemia Reperfusion

Abstract Objective The effects of urapidil in intestinal ischemia-reperfusion (IR) model were investigated using histopathological and biochemical methods. Materials and methods Forty Wistar albino rats were subjected to sham operation (Group 1), IR (Group 2), IR+dimethyl sulfoxide (Group 3), IR+urapidil 0.5 mg/kg (Group 4), and IR+urapidil 5 mg/kg (Group 5). Levels of MDA, TAS, TOS, SOD, MPO, NF-κB, caspase-3, and LC3B were measured. Results and discussion The groups 2 and 3 had significantly higher TOS and MPO levels than the sham group had (p < 0.001), whereas the TAS and SOD levels were significantly lower in Group 2 than in the sham group. In treatment groups, TAS and SOD levels increased, whereas TOS, MPO, and MDA levels decreased compared to Group 2. Caspase-3 and LC3B immunopositivities were seen at severe levels in Group 2 and 3. However, Group 4 and 5 were found to have lower levels of immunopositivity. Immunopositivity was observed in interstitial areas, peribronchial region, and bronchial epithelial cells. A moderate level of NF-κB immunopositivity was seen in Group 2 and 3. Conclusion Our results show that urapidil is one of the antioxidant agents and protects lung tissue from oxidant effects of intestinal IR injury.

scholarly journals Gene expression profile of oxidative stress in the lung of inbred mice after intestinal ischemia/reperfusion injury

2014 ◽  
Vol 29 (3) ◽  
pp. 186-192 ◽  
Author(s):  
Adauto Tsutomu Ikejiri ◽  
Frederico Somaio Neto ◽  
José Carlos Chaves ◽  
Paulo Roberto Bertoletto ◽  
Roberto Teruya ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Reperfusion Injury ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Intestinal Ischemia ◽  
Ischemia Reperfusion Injury ◽  
Inbred Mice ◽  
Ischemia Reperfusion ◽  
Intestinal Ischemia Reperfusion
Sign in / Sign up

Export Citation Format

Share Document