scholarly journals Green synthesis of silver nanoparticles using leaf extract from Tabebuia roseoalba and T. pentaphylla

Author(s):  
Laureen Michelle Houllou ◽  
Robson Antonio Barbosa De Souza ◽  
Carolina Barbosa Malafaia ◽  
Débora Lorrane Montenegro da Paixão ◽  
Alisson Tito Bezerra de Araújo ◽  
...  

Metal nanoparticles are nanostructures that can be applied to biotechnology because they present different biological activities. Among them, the silver nanoparticles (AgNPs) are known to present antimicrobial activity allowing their application in several areas such as medicine and industry. The biological synthesis of AgNPs is ecologically correct and advantageous techniques. The objective of this work was to evaluate the synthesis of AgNps through the green synthesis using extracts of leaves of Tabebuia roseoalba and T. pentaphylla grown in vivo and in vitro. The nanoparticle synthesis solution was colorimetrically evaluated, and the nanoparticles were physically characterized. The results obtained demonstrate that both extracts of both Tabebuia species tested are able to synthesize AgNPs, however only when cultured under in vivo conditions. These data suggest that photosynthesis under natural conditions promotes the production of metabolites that are essential to green synthesis.

2019 ◽  
Vol 31 (2) ◽  
pp. 463-477 ◽  
Author(s):  
Ningaraju Sunayana ◽  
Munawer Uzma ◽  
Rudra Prasad Dhanwini ◽  
Mellappa Govindappa ◽  
Harischandra Sripathy Prakash ◽  
...  

Author(s):  
Irfana Zahoor ◽  
Farhat Jan ◽  
Ujjawal Sharma ◽  
Kiran K. Sahu ◽  
Amita Sharma ◽  
...  

Background:: There is an urgent need to devise improved alternatives for the efficient delivery of drugs to develop improved therapeutic interventions against cancers. Nanotechnology based drug delivery vehicles are in-use with obvious issues of toxicity and bio-distribution. Therefore, green synthetic routes are being deployed to replace the conventional nanoparticle formulations for effective drug delivery aiming at developing interventional strategies against cancer. Objective:: A simple, viable and fast approach was used for the green synthesis of silver nanoparticles (AgNPs) using aqueous leaf-extract of Viburnum nervosum (VN) and to explore the anti-cancer potential of the crude extract of VN. Methods:: Silver NPs were synthesized by reacting silver nitrate (AgNO3) with leaf extract of VN. Various analytical techniques were used to characterize the AgNPs. Finally, the anti-cancer potential of VN was observed when delivered through AgNPs. Results:: The surface plasmon spectra for AgNPs exhibited absorbance peak at 445 nm, and Fourier-Transform Infrared Spectroscopy investigation revealed presence of biomolecules acting as an effective reducing and capping agent for converting silver nitrate to AgNPs. Further, our results suggest the spherical size of synthesized AgNPs ranging from 12-17 nm. Moreover, in vitro studies conducted for VN extract with breast (MCF-7) and epidermal carcinoma (A431) cells showed biocompatibility. Conclusion:: Doxorubicin loaded AgNPs documented an increased bioavailability of drug compared to the free drug, suggesting the use of AgNPs as “novel drug delivery vectors”.


2020 ◽  
Vol 2 (1) ◽  
pp. 93

Graphene oxide nanoparticles has found immense application in bio therapeutics owing to its biocompatibility and enhanced effectiveness in drug delivery. The present study investigates the green synthesis of reduced graphene oxide using leaf extract using Tridax procumbens and testing its in-vitro biological activities. The biosynthesized reduced graphene oxide (rGO) was characterized by various spectroscopic and microscopic techniques. UV-vis spectroscopic analysis primarily detected the shift in the absorption peak from 232 to 287 nm confirming the reduction of TP-rGO. FTIR spectra of TP-rGO confirmed effective deoxygenation of GO assisted by bioactive molecules present in the leaf extract. Raman spectroscopic analysis identified successful reduction of GO through the presence of D band at 1329 cm-1 and G band at 1577 cm-1. XRD spectra of TP-rGO showed diffraction peak 2θ at 25o confirming the presence of reduced GO. SEM analysis revealed the presence of graphene nanosheets appearing as large aggregated structure. The bioreduced rGO showed anti-oxidant effect against DPPH radical observed in concentration dependent manner. The TP-rGO nanosheets exhibited bactericidal effect against gram positive bacteria: B.subitils (18 mm) and S. epidermis (9 mm) and gram negative bacteria: E.coli (14 mm) and P. aeruginosa (6 mm) at 100 µg/ml confirming the enhanced anti-bacterial effects of TP-rGO against gram positive pathogens. Hence, it is highlighted that leaf extract of Tridax procumbens act as green reducing agent for the successful biosynthesis of rGO nanosheets demonstrating potential in-vitro biological activities and has great scope in the preparation of nano drugs for the treatment of various diseases.


2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Le Thi An Nhien ◽  
Nguyen Duc Luong ◽  
Le Thi Thuy Tien ◽  
Le Quang Luan

Silver nanoparticles (AgNPs) were successfully prepared by γ-rays irradiation of solution containing 1.0–10 mM of silver nitrate and 1% chitosan. The optical characteristics and particles sizes of AuNPs were determined by UV-Vis spectra and TEM images, respectively. The size of AgNPs increased by the increase of silver concentration or the decrease of chitosan molecular weight in irradiated solution. The in vitro test showed that AgNPs inhibited the growth of Corynespora cassiicola on rubber-leaf extract media with the inhibitory efficiency of 52.1–100% by treatment of AgNPs with particle size from 15 to 5 nm, respectively. In addition, antifungal activity was found to reach ~100% by the addition of 90 ppm AgNPs. The in vivo foliar treatment of AgNPs on 9-month-old rubber plants showed that the treatment with 2.5–12.5 ppm AgNPs on tested plants after inoculation by spraying with C. cassiicola spores enhanced the rate of non-disease-infected plants from 6.0 to 93.3%, respectively, compared to the untreated control. The inhibition effect of AgNPs on fungal growth of C. cassiicola mycelial was also elucidated via SEM images. The AgNPs/chitosan synthesized by γ-irradiation is potentially promising to use as a fungicidal product for treating C. cassiicola, a serious pathogen fungus on rubber trees.


2019 ◽  
Vol 31 (7) ◽  
pp. 1447-1453
Author(s):  
Nagarajan Padmini ◽  
Nagasundaram Rashiya ◽  
Natesan Sivakumar ◽  
Narayanan Dhiraviam Kannan ◽  
Ramamoorthy Manjuladevi ◽  
...  

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