Green Synthesis of Gold Nanoparticles from Vitex negundo Leaf Extract to Inhibit Lipopolysaccharide-Induced Inflammation Through In Vitro and In Vivo

The main aim of the study was to optimization and evaluation of a suitable dosage form i.e., tropical gel containing silver and gold nanoparticles which are synthesized by green synthesis as this is the rapid and better alternative to chemical synthesis. For burn injury systemic route of administration is not suitable due to damaged skin hence tropical route selected to provide controlled effects, low systemic toxicity and easy application. Silver nanoparticles were synthesized by green synthesis by use of neem leaves and gold nanoparticles by Aloe vera. Characterization was done by UV spectroscopy, particle size distribution, TEM and zeta potential. Gel was formulated by simple mixing and stirring method and evaluated for color, homogeneity, Spreadability, viscosity, in vitro release, antimicrobial study and in vivo study. In this study, the combination of Carbopol 934 P and HPMC (ratio 1:3) was found to be ideal formulation (Formulation F5) with optimum viscosity and Spread-ability i.e., 4459 cP and 5.7 gmcm/sec resp. in vitro release was found to be 98.66% in 12 hours. in vitro antimicrobial study and in vivo study on rat showed maximum effects than standard formulations. From this study it can be concluded that, tropical gel is the superior dosage form containing silver and gold nanoparticles which acts as the antimicrobial agents and is effective in treatment of burn surgery.

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Green synthesis of silver nanoparticles using leaf extract from Tabebuia roseoalba and T. pentaphylla

Journal of Environmental Analysis and Progress ◽

10.24221/jeap.4.3.2019.2523.216-222 ◽

2019 ◽

pp. 216-222

Author(s):

Laureen Michelle Houllou ◽

Robson Antonio Barbosa De Souza ◽

Carolina Barbosa Malafaia ◽

Débora Lorrane Montenegro da Paixão ◽

Alisson Tito Bezerra de Araújo ◽

...

Keyword(s):

Silver Nanoparticles ◽

Green Synthesis ◽

Leaf Extract ◽

Biological Activities ◽

Nanoparticle Synthesis ◽

Biological Synthesis ◽

Natural Conditions ◽

Synthesis Solution

Metal nanoparticles are nanostructures that can be applied to biotechnology because they present different biological activities. Among them, the silver nanoparticles (AgNPs) are known to present antimicrobial activity allowing their application in several areas such as medicine and industry. The biological synthesis of AgNPs is ecologically correct and advantageous techniques. The objective of this work was to evaluate the synthesis of AgNps through the green synthesis using extracts of leaves of Tabebuia roseoalba and T. pentaphylla grown in vivo and in vitro. The nanoparticle synthesis solution was colorimetrically evaluated, and the nanoparticles were physically characterized. The results obtained demonstrate that both extracts of both Tabebuia species tested are able to synthesize AgNPs, however only when cultured under in vivo conditions. These data suggest that photosynthesis under natural conditions promotes the production of metabolites that are essential to green synthesis.

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Dosimetry and Radioenhancement Comparison of Gold Nanoparticles in Kilovoltage and Megavoltage Radiotherapy using MAGAT Polymer Gel Dosimeter

Journal of Biomedical Physics and Engineering ◽

10.31661/jbpe.v9i2apr.827 ◽

2019 ◽

Vol 9 (2Apr) ◽

Cited By ~ 1

Author(s):

S Farahani ◽

N Riyahi Alam ◽

S Haghgoo ◽

M Khoobi ◽

Gh Geraily ◽

...

Keyword(s):

Gold Nanoparticles ◽

3D Visualization ◽

Energy Levels ◽

Polymer Gel ◽

External Radiotherapy ◽

Dose Enhancement ◽

Internal Radiotherapy ◽

Boost Radiotherapy

Background: Numerous unique characteristics of the nanosized gold, including high atomic number, low toxicity, and high biocompatibility make it one of the most appropriate nanostructures to boost radiotherapy efficacy. Many in-vivo and in-vitro investigations have indicated that gold nanoparticles (AuNPs) can significantly increase tumor injuries in low kilovoltage radiotherapy. While deep-lying tumors require much higher energy levels with greater penetration power, and investigations carried out in megavoltage energy range show contradictory results.Objective: In this study, we quantitatively assess and compare dose enhancement factors (DEFs) obtained through AuNPs under radiation of Cobalt-60 source (1.25MeV) versus Iridium-192 source (0.380 KeV) using MAGAT gel dosimeter.Material and Methods: MAGAT polymer gel in both pure and combined with 0.2 mM AuNPs was synthesized. In order to quantify the effect of energy on DEF, irradiation was carried out by Co-60 external radiotherapy and Ir-192 internal radiotherapy. Finally, readings of irradiated and non-irradiated gels were performed by MR imaging.Result: The radiation-induced R2 (1/T2) changes of the gel tubes doped with AuNPs compared to control samples, upon irradiation of beams released by Ir-192 source showed a significant dose enhancement (15.31% ±0.30) relative to the Co-60 external radiotherapy (5.85% ±0.14).Conclusion: This preliminary study suggests the feasibility of using AuNPs in radiation therapy (RT), especially in low-energy sources of brachytherapy. In addition, MAGAT polymer gel, as a powerful dosimeter, could be used for 3D visualization of radiation dose distribution of AuNPs in radiotherapy.

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Radiosensitization of Prostate Cancers In Vitro and In Vivo to Erbium-filtered Orthovoltage X-rays Using Actively Targeted Gold Nanoparticles

Scientific Reports ◽

10.1038/s41598-017-18304-y ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 12

Author(s):

Allison M. Khoo ◽

Sang Hyun Cho ◽

Francisco J. Reynoso ◽

Maureen Aliru ◽

Kathryn Aziz ◽

...

Keyword(s):

Gold Nanoparticles ◽

X Rays ◽

Prostate Cancers

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Biocompatibility of gold nanoparticles: In-vitro and In-vivo study

Materials Today Proceedings ◽

10.1016/j.matpr.2020.12.826 ◽

2021 ◽

Vol 42 ◽

pp. 3041-3045

Author(s):

Rua J. Kadhim ◽

Esraa H. Karsh ◽

Zainab J. Taqi ◽

Majid S. Jabir

Keyword(s):

Gold Nanoparticles ◽

In Vivo Study

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In Vitro and In Vivo Antioxidant Properties of Taraxacum officinale in Nω-Nitro-l-Arginine Methyl Ester (L-NAME)-Induced Hypertensive Rats

Antioxidants ◽

10.3390/antiox8080309 ◽

2019 ◽

Vol 8 (8) ◽

pp. 309

Author(s):

Olukayode O. Aremu ◽

Adebola O. Oyedeji ◽

Opeoluwa O. Oyedeji ◽

Benedicta N. Nkeh-Chungag ◽

Constance R. Sewani Rusike

Keyword(s):

Oxidative Stress ◽

Free Radical ◽

Methyl Ester ◽

Leaf Extract ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Hypertensive Rats ◽

Total Antioxidant

Oxidative stress has gained attention as one of the fundamental mechanisms responsible for the development of hypertension. The present study investigated in vitro and in vivo antioxidant effects of 70% ethanol-water (v/v) leaf and root extracts of T. officinale (TOL and TOR, respectively). Total phenolic and flavonoid content of plant extracts were assessed using Folin Ciocalteau and aluminium chloride colorimetric methods; while, 2,2-diphenyl-1-picrlhydrazyl (DPPH), 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and ferric reducing antioxidant power (FRAP) protocols were used to determine the free radical scavenging and total antioxidant capacities (TAC), respectively. The in vivo total antioxidant capacity and malondialdehyde acid (MDA) levels for lipid peroxidation tests were performed on organ homogenate samples from Nω-nitro-L-arginine methyl ester (L-NAME)-induced hypertensive rats treated with leaf extract, TOL (500 mg/kg/day) and TOR (500 mg/kg/day) for 21 days. Results showed that compared to TOR, TOL possessed significantly higher (p < 0.01) polyphenol (4.35 ± 0.15 compared to 1.14 ± 0.01) and flavonoid (23.17 ± 0.14 compared to 3 ± 0.05) content; free radical scavenging activity (EC50 0.37 compared to 1.34 mg/mL) and total antioxidant capacities (82.56% compared to 61.54% ABTS, and 156 ± 5.28 compared to 40 ± 0.31 FRAP) and both extracts showed no toxicity (LD50 > 5000 mg/kg). TOL and TOR significantly (p < 0.01) elevated TAC and reduced MDA levels in targets organs. In conclusion, T. officinale leaf extract possesses significant anti-oxidant effects which conferred significant in vivo antioxidant protection against free radical-mediated oxidative stress in L-NAME-induced hypertensive rats.

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