scholarly journals Propagation of Selected Clones of Eastern Redbud (Cercis canadensis) by Stem Cuttings

2009 ◽  
Vol 27 (1) ◽  
pp. 12-16
Author(s):  
John M. Wooldridge ◽  
Frank A. Blazich ◽  
Stuart L. Warren
Keyword(s):  
Growth Stage ◽  
The Other ◽  
Stem Cuttings ◽  
Indolebutyric Acid ◽  
Auxin Treatment ◽  
Cercis Canadensis ◽  
Eastern Redbud ◽  
F1 Generation

Abstract Two experiments, one utilizing softwood cuttings and the other semi-hardwood cuttings, were conducted to investigate the influence of growth stage and auxin treatment on rooting four related clones of eastern redbud (Cercis canadensis L.). The four clones were ‘Flame’ (C. canadensis L. ‘Flame’), dwarf white, and two selections (NC99-6-1 and NC99-6-2) of an F1 generation derived from a cross of ‘Flame’ and dwarf white. At each growth stage, rooting responses of the clones varied and were influenced greatly by auxin treatment, demonstrating the variable rooting potential of the genotypes. In both studies, ‘Flame’ rooted well [63% rooting in the softwood study when treated with the potassium (K) salt (K-salt) of indolebutyric acid (IBA) at 5000 mg·L−1 (ppm), 83% rooting when treated in the semi-hardwood study with K-IBA at 10,000 mg·L−1], indicating stem cuttings may be a commercially feasible means of propagation for ‘Flame.’ Softwood cuttings of dwarf white (46%) and NC99-6-2 (75%) rooted best when treated with K-IBA at 15,000 mg·L−1, whereas softwood cuttings of NC99-6-1 rooted best (46%) when treated with K-IBA at 10,000 mg·L−1. Dwarf white and the F1s rooted poorly in the semi-hardwood study.

Propagation of Thuja × `Green Giant' by Stem Cuttings: Effects of Growth Stage, Type of Cutting, and IBA Treatment

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 504a-504 ◽  
Author(s):  
Jason J. Griffin ◽  
Frank A. Blazich ◽  
Thomas G. Ranney
Keyword(s):  
Root Length ◽  
Growth Stage ◽  
Specific Growth ◽  
Growth Stages ◽  
Stem Cuttings ◽  
Indolebutyric Acid ◽  
Rooted Cutting ◽  
Auxin Treatment

Stem cuttings of Thuja L. × `Green Giant', consisting of 22-cm terminals or 20-cm laterals, were collected on three dates associated with specific growth stages (softwood, semi-hardwood, and hardwood), and treated with indolebutyric acid (IBA) in 50% isopropanol ranging from 0 to 9000 ppm and placed under intermittent mist. Regardless of cutting type or auxin treatment, cuttings rooted in high percentages at each growth stage. Overall rooting was highest for hardwood cuttings (96%) followed by semi-hardwood (86%), and softwood (85%) cuttings. Semi-hardwood cuttings were the only cuttings in which rooting was affected by IBA or cutting type with the greatest rooting (94%) attained using lateral cuttings treated with 6000 or 9000 ppm IBA. Although an interaction occurred between IBA treatment and cutting type for mean root length of hardwood cuttings, only mean root length of semi-hardwood cuttings was influenced by IBA concentration alone with the greatest length (25 mm) achieved with 6000 ppm IBA. An interaction was noted for root count between cutting type and IBA concentrations for softwood cuttings, whereas IBA and cutting type affected independently root count on semi-hardwood cuttings. Treatment with 9000 ppm IBA resulted in 18 roots per rooted cutting whereas lateral cuttings responded with a mean of 16 roots over all IBA concentrations. Root count on hardwood cuttings was affected by IBA treatment only, with 9000 ppm being optimum (14 roots per rooted cutting).

scholarly journals Propagation of ‘Carolina Sapphire’ Smooth Arizona Cypress by Stem Cuttings: Effects of Growth Stage, Type of Cutting, and IBA Treatment

1997 ◽  
Vol 15 (2) ◽  
pp. 61-64
Author(s):  
Hunter L. Stubbs ◽  
Frank A. Blazich ◽  
Thomas G. Ranney ◽  
Stuart L. Warren
Keyword(s):  
Growth Stage ◽  
Specific Growth ◽  
Dry Weight ◽  
Growth Stages ◽  
Stem Cuttings ◽  
Indolebutyric Acid ◽  
Root Dry Weight ◽  
Auxin Treatment ◽  
Cupressus Arizonica

Abstract Stem cuttings of ‘Carolina Sapphire’ smooth Arizona cypress [Cupressus arizonica var. glabra (Sudw.) Little ‘Carolina Sapphire’] consisting of 30 cm (12 in) terminals or distal [terminal 15 cm (6 in)] and proximal [basal 15 cm (6 in)] halves of 30 cm (12 in) terminals were taken on three dates associated with specific growth stages (semi-hardwood, hardwood, and softwood). Cuttings were treated with indolebutyric acid (IBA) in 50% isopropanol ranging from 0 to 16,000 ppm (1.6%) and placed under intermittent mist. Regardless of cutting type and auxin treatment, cuttings rooted at each growth stage. Overall percent rooting was highest during the hardwood stage (70%), followed by the semi-hardwood stage (44%). Softwood cuttings exhibited the lowest overall rooting (33%). At each growth stage, percent rooting, root count, and root dry weight varied depending on cutting type and IBA treatment. Rooting percentages ≥70% were attained at the semi-hardwood and hardwood growth stages for particular treatment combinations.

scholarly journals Propagation of Eastern Redbud (Cercis canadensis) by Stem Cuttings is Influenced by Clone and Date of Cutting Collection

2009 ◽  
Vol 27 (2) ◽  
pp. 109-114
Author(s):  
John M. Wooldridge ◽  
Frank A. Blazich ◽  
Stuart L. Warren
Keyword(s):  
Root Systems ◽  
Stem Cuttings ◽  
Indolebutyric Acid ◽  
Optimum Time ◽  
Cercis Canadensis ◽  
Eastern Redbud ◽  
Collection Date

Abstract Stem cuttings of four popular clones of eastern redbud (Cercis canadensis L. ‘Ace of Hearts’, ‘Appalachian Red’, ‘Hearts of Gold’, and ‘Forest Pansy’) were taken on seven dates following budbreak during Spring and Summer 2007 and evaluated for rooting potential. Rooting was affected by a clone and cutting date interaction, indicating the optimum time to take cuttings was different for each clone. Cuttings of ‘Ace of Hearts’ taken 6 weeks after budbreak (WAB) rooted at 75 and 71% when treated with the potassium (K) salt (K-salt) of indolebutyric acid (K-IBA) at 5000 mg·liter−1 (ppm) or 15,000 mg·liter−1, respectively. In contrast, cuttings of ‘Appalachian Red’ rooted at 96 and 93% when taken 15 WAB, the last date tested for that clone, and treated with K-IBA at 5000 mg·liter−1 or 15,000 mg·liter−1, respectively. When taken 8 WAB and treated with K-IBA at 5000 mg·liter−1 or 15,000 mg·liter−1, cuttings of ‘Hearts of Gold’ rooted at 42 and 58%, respectively. Cuttings of ‘Forest Pansy’ rooted poorly regardless of collection date or K-IBA treatment. Treatment of ‘Ace of Hearts’, ‘Appalachian Red’, and ‘Hearts of Gold’ with higher K-IBA rates generally did not increase rooting percentages, but often resulted in more robust root systems. Propagation by stem cuttings may be feasible for some clones of eastern redbud, but separate protocols are necessary for each clone.

scholarly journals Micropropagation of White Flowering Eastern Redbud (Cercis canadensis var. alba L.)

1990 ◽  
Vol 8 (4) ◽  
pp. 177-179
Author(s):  
S. Yusnita ◽  
R. L. Geneve ◽  
S. T. Kester
Keyword(s):  
Root System ◽  
Day Treatment ◽  
Shoot Multiplication ◽  
Naphthaleneacetic Acid ◽  
Indolebutyric Acid ◽  
Cercis Canadensis ◽  
Half Strength ◽  
Eastern Redbud ◽  
Being Moved

Abstract A white flowering Eastern redbud (Cercis canadensis var. alba L.) has been successfully micropropagated. Two node explants collected from the initial flush of spring growth were cultured on woody plant medium (WPM). Increased shoot multiplication occurred at 10,15 and 20 μM (2.3, 3.4 and 4.5 ppm) benzyladenine (BA). Microshoots were rooted in vitro on half strength WPM with a 15-day treatment of 100 and 300 μM (18.6 and 55.9 ppm) α-naphthaleneacetic acid (NAA) or 100 and 300 μM (20.3 and 60.9 ppm) indolebutyric acid (IBA) prior to being moved to full strength WPM without growth regulators. Percentage rooting and the mean number of roots per cutting were comparable between NAA and IBA treated microcuttings, however, the subsequent root morphology differed between the two treatments. NAA treated plants developed a coarse, unbranched root system, while IBA treated cuttings developed a more desireable fine, branched root system. Rooted microshoots were successfully acclimated to greenhouse conditions.

scholarly journals Shading and IBA Treatment Do Not Improve Rooting of Stem Cuttings of Quercus phillyraeoides ‘Emerald Sentinel’

1999 ◽  
Vol 17 (3) ◽  
pp. 123-125
Author(s):  
Jason J. Griffin ◽  
Frank A. Blazich ◽  
Thomas G. Ranney
Keyword(s):  
Solar Irradiance ◽  
Root Length ◽  
Growth Stage ◽  
Shoot Tip ◽  
Stem Cuttings ◽  
Root Number ◽  
Indolebutyric Acid ◽  
Rooted Cutting ◽  
Quercus Phillyraeoides

Abstract Shoot tip cuttings of Quercus phillyraeoides A. Gray ‘Emerald Sentinel’ (‘Emerald Sentinel’ oak), in a transitional growth stage between softwood and semi-hardwood, were collected from containerized plants growing under uniform fertility on June 4, 1998. Cuttings were treated with talc formulations of indolebutyric acid (IBA), ranging from 0 to 8000 ppm (0.8%), and placed under intermittent mist with shade levels excluding 0%, 30%, 60% or 90% of full solar irradiance. After 14 weeks, percent rooting averaged 18% and was unaffected by either IBA concentration or shading regime. Similarly, both mean root number per rooted cutting and mean root length were unaffected by IBA concentration or shading. Roots per rooted cutting and mean root length averaged 2.6 and 41 mm (1.6 in), respectively. Results suggest that shading stem cuttings of ‘Emerald Sentinel’ during propagation does not affect rooting.

scholarly journals Propagation of Ilex vomitoria ‘Dare County’ by Stem Cuttings

2012 ◽  
Vol 30 (2) ◽  
pp. 55-57 ◽  
Author(s):  
Christopher R. Lambert ◽  
Frank A. Blazich ◽  
Anthony V. LeBude
Keyword(s):  
Stem Cuttings ◽  
Indolebutyric Acid ◽  
Auxin Treatment ◽  
Linear Decrease ◽  
Ilex Vomitoria

Semi-hardwood or hardwood stem cuttings of Ilex vomitoria Sol. ex Ait. ‘Dare County’ [‘Dare County’ yaupon holly (syn. ‘Virginia Dare’ yaupon holly)] were treated with solutions of the potassium (K) salt (K-salt) of indolebutyric acid (K-IBA) at 0 to 8000 mg liter−1 (ppm). Nontreated semi-hardwood cuttings rooted at 78% whereas, regardless of auxin treatment, hardwood cuttings taken on two dates rooted at 15%. Treatment of cuttings with K-IBA was generally ineffective and resulted in a linear decrease (P ≤ 0.05) in percent rooting of semi-hardwood cuttings.

scholarly journals Propagation of Quercus myrsinifolia and Quercus canbyi by Stem Cuttings

1996 ◽  
Vol 14 (4) ◽  
pp. 217-220 ◽  
Author(s):  
Patrick J. McGuigan ◽  
Frank A. Blazich ◽  
Thomas G. Ranney
Keyword(s):  
Growth Phase ◽  
Growth Stage ◽  
Specific Growth ◽  
Growth Stages ◽  
Transitional Stage ◽  
Stem Cuttings ◽  
Indolebutyric Acid ◽  
Adult Growth ◽  
Root Responses ◽  
Evergreen Oak

Abstract Stem cuttings of two clones (clone 1 and 2) of Quercus myrsinifolia Bl. (Chinese evergreen oak), and one clone of Quercus canbyi Trel. (Canby's oak), of seedling origin and in the adult growth phase, were taken on various dates representing specific growth stages. Cuttings of clone 1 of Q. myrsinifolia were collected at the semi-hardwood, hardwood, or softwood stages in addition to a transitional stage between softwood and semi-hardwood. For clone 2, stem cuttings were taken only at the softwood and transitional softwood/semi-hardwood stages. Cuttings of Q. canbyi were collected at the semi-hardwood, hardwood, or softwood stages. Cuttings of both species were treated with 0, 1500 (0.15%), 3000 (0.3%), 6000 (0.6%), or 9000 ppm (0.9%) indolebutyric acid (IBA) in 50% isopropanol. All cuttings were placed in a raised greenhouse bench and rooted under intermittent mist. Semi-hardwood or hardwood cuttings of Q. myrsinifolia or Q. canbyi did not root. Responses of stem cuttings of Q. myrsinifolia to IBA treatment varied by growth stage. For softwood cuttings response to IBA was quadratic with the greatest rooting noted for clones 1 (56%) and 2 (72%) when treated with 1500 and 3000 ppm IBA, respectively. Treatment with IBA had no effect on percent rooting of softwood/semi-hardwood cuttings of clone 1, with rooting ranging from 47% to 58%. However, a linear decrease in rooting in response to IBA was observed fordone 2 with the greatest rooting occurring for the nontreated cuttings (89%). Softwood cuttings of Q. canbyi responded quadratically to IBA treatment, with maximum rooting of 33% noted for cuttings treated with 1500 ppm IBA.

scholarly journals Effects of Wounding and Auxin Treatment on Rooting Stem Cuttings of Fraser's Photinia

1983 ◽  
Vol 1 (4) ◽  
pp. 104-106
Author(s):  
Frank A. Blazich ◽  
Vincent P. Bonaminio
Keyword(s):  
Stem Cuttings ◽  
Indolebutyric Acid ◽  
Auxin Treatment ◽  
Photinia X Fraseri

Light or heavy wounding applied to stem cuttings of Fraser's photinia (Photinia x fraseri Dress) had little effect on stimulating rooting. The greatest response from wounding was realized when used in combination with a 5000 or 10,000 ppm indolebutyric acid (IBA) solution. Satisfactory rooting, however, was attained by IBA treatment alone.

scholarly journals Propagation of Thuja x ‘Green Giant’ by Stem Cuttings: Effects of Growth Stage, Type of Cutting, and IBA Treatment

1998 ◽  
Vol 16 (4) ◽  
pp. 212-214
Author(s):  
Jason J. Griffin ◽  
Frank A. Blazich ◽  
Thomas G. Ranney
Keyword(s):  
Root Length ◽  
Growth Stage ◽  
Growth Stages ◽  
Stem Cuttings ◽  
Root Number ◽  
Stock Plant ◽  
Indolebutyric Acid ◽  
First Order ◽  
Rooting Medium ◽  
Plant Growth Stages

Abstract Stem cuttings of Thuja L. x ‘Green Giant’ (‘Green Giant’ arborvitae), consisting of 22 cm (8.6 in) terminals (tips of first-order laterals) or 20 cm (7.8 in) laterals (side shoots removed from those portions of terminal cuttings inserted into the rooting medium), were collected on three dates associated with specific stock plant growth stages (softwood, semi-hardwood, and hardwood) and treated with indolebutyric acid (IBA) in 50% isopropanol ranging from 0 to 9000 ppm (0.9%). Regardless of cutting type or auxin treatment, cuttings rooted in high percentages at each growth stage. Overall rooting for softwood, semi-hardwood, and hardwood, cuttings was 85%, 86%, and 96%, respectively. Semi-hardwood cuttings were the only cuttings in which percent rooting was affected by IBA treatment or cutting type, with lateral cuttings rooting in higher percentages than terminal cuttings (92% vs. 79%, respectively), while IBA concentrations of 3000 (0.3%) or 6000 ppm (0.6%) proved optimal for both cutting types. Mean root number and mean root length varied by growth stage, cutting type, and IBA concentration. In general, rooting percentages and root length were optimized at the hardwood stage with either cutting type treated with 3000, 6000 or 9000 ppm (0.3%, 0.6% or 0.9%) IBA, resulting in >10 roots per cutting.

scholarly journals Propagation of Quercus robur L. (English Oak) by stem cuttings in Western Himalayas (Kashmir)

2014 ◽  
Vol 15 (1&2) ◽  
pp. 185-189
Author(s):  
Javaid Iqbal ◽  
V. Dutt ◽  
Hillal Ahmad ◽  
G.M. Bhat ◽  
P. A. Khan
Keyword(s):  
Acetic Acid ◽  
Quercus Robur ◽  
Naphthalene Acetic Acid ◽  
Western Himalayas ◽  
Stem Cuttings ◽  
Kashmir Valley ◽  
Indolebutyric Acid ◽  
Auxin Treatment ◽  
English Oak

Two experiments were conducted for propagation of Quercus robur L. by stem cuttings in Kashmir valley. First experiment which includes: hardwood cuttings were treated with Indolebutyric acid(IBA)having concentrations of 5,000, 10,000, 15,000, and 20,000 ppm in talc and Naphthalene acetic acid (NAA) having concentrations of 500, 1,000 and 1,500 ppm in talc while the second experiment i.e. softwood cuttings were taken and treated with Indolebutyric acid (IBA) having concentrations of 5,000, 10,000, 15,000, 20,000 ppm quick-dipped for 5-seconds and Naphthalene acetic acid (NAA) having concentrations of500, 1,000 and 1,500 ppm dipped for 24 hours and in both (nontreated) as control and placed under mist conditions. It was found that English Oak can be propagated through cuttings and auxin treatment is imperative and Indolebutyric acid (IBA) with concentration of 10,000 ppm showed best results with highest recorded rooting of 51.30 per cent in case of softwood cuttings and for hardwood cuttings also, Indolebutyric acid (IBA) with concentration of 10,000 ppm showed good results with highest recorded rooting of 29.70 per cent. However, control and NAA-treatments in both softwood and hardwood cuttings could not induce rooting at all.

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