scholarly journals Propagation of ‘Carolina Sapphire’ Smooth Arizona Cypress by Stem Cuttings: Effects of Growth Stage, Type of Cutting, and IBA Treatment

1997 ◽  
Vol 15 (2) ◽  
pp. 61-64
Author(s):  
Hunter L. Stubbs ◽  
Frank A. Blazich ◽  
Thomas G. Ranney ◽  
Stuart L. Warren

Abstract Stem cuttings of ‘Carolina Sapphire’ smooth Arizona cypress [Cupressus arizonica var. glabra (Sudw.) Little ‘Carolina Sapphire’] consisting of 30 cm (12 in) terminals or distal [terminal 15 cm (6 in)] and proximal [basal 15 cm (6 in)] halves of 30 cm (12 in) terminals were taken on three dates associated with specific growth stages (semi-hardwood, hardwood, and softwood). Cuttings were treated with indolebutyric acid (IBA) in 50% isopropanol ranging from 0 to 16,000 ppm (1.6%) and placed under intermittent mist. Regardless of cutting type and auxin treatment, cuttings rooted at each growth stage. Overall percent rooting was highest during the hardwood stage (70%), followed by the semi-hardwood stage (44%). Softwood cuttings exhibited the lowest overall rooting (33%). At each growth stage, percent rooting, root count, and root dry weight varied depending on cutting type and IBA treatment. Rooting percentages ≥70% were attained at the semi-hardwood and hardwood growth stages for particular treatment combinations.

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 504a-504 ◽  
Author(s):  
Jason J. Griffin ◽  
Frank A. Blazich ◽  
Thomas G. Ranney

Stem cuttings of Thuja L. × `Green Giant', consisting of 22-cm terminals or 20-cm laterals, were collected on three dates associated with specific growth stages (softwood, semi-hardwood, and hardwood), and treated with indolebutyric acid (IBA) in 50% isopropanol ranging from 0 to 9000 ppm and placed under intermittent mist. Regardless of cutting type or auxin treatment, cuttings rooted in high percentages at each growth stage. Overall rooting was highest for hardwood cuttings (96%) followed by semi-hardwood (86%), and softwood (85%) cuttings. Semi-hardwood cuttings were the only cuttings in which rooting was affected by IBA or cutting type with the greatest rooting (94%) attained using lateral cuttings treated with 6000 or 9000 ppm IBA. Although an interaction occurred between IBA treatment and cutting type for mean root length of hardwood cuttings, only mean root length of semi-hardwood cuttings was influenced by IBA concentration alone with the greatest length (25 mm) achieved with 6000 ppm IBA. An interaction was noted for root count between cutting type and IBA concentrations for softwood cuttings, whereas IBA and cutting type affected independently root count on semi-hardwood cuttings. Treatment with 9000 ppm IBA resulted in 18 roots per rooted cutting whereas lateral cuttings responded with a mean of 16 roots over all IBA concentrations. Root count on hardwood cuttings was affected by IBA treatment only, with 9000 ppm being optimum (14 roots per rooted cutting).


1996 ◽  
Vol 14 (4) ◽  
pp. 217-220 ◽  
Author(s):  
Patrick J. McGuigan ◽  
Frank A. Blazich ◽  
Thomas G. Ranney

Abstract Stem cuttings of two clones (clone 1 and 2) of Quercus myrsinifolia Bl. (Chinese evergreen oak), and one clone of Quercus canbyi Trel. (Canby's oak), of seedling origin and in the adult growth phase, were taken on various dates representing specific growth stages. Cuttings of clone 1 of Q. myrsinifolia were collected at the semi-hardwood, hardwood, or softwood stages in addition to a transitional stage between softwood and semi-hardwood. For clone 2, stem cuttings were taken only at the softwood and transitional softwood/semi-hardwood stages. Cuttings of Q. canbyi were collected at the semi-hardwood, hardwood, or softwood stages. Cuttings of both species were treated with 0, 1500 (0.15%), 3000 (0.3%), 6000 (0.6%), or 9000 ppm (0.9%) indolebutyric acid (IBA) in 50% isopropanol. All cuttings were placed in a raised greenhouse bench and rooted under intermittent mist. Semi-hardwood or hardwood cuttings of Q. myrsinifolia or Q. canbyi did not root. Responses of stem cuttings of Q. myrsinifolia to IBA treatment varied by growth stage. For softwood cuttings response to IBA was quadratic with the greatest rooting noted for clones 1 (56%) and 2 (72%) when treated with 1500 and 3000 ppm IBA, respectively. Treatment with IBA had no effect on percent rooting of softwood/semi-hardwood cuttings of clone 1, with rooting ranging from 47% to 58%. However, a linear decrease in rooting in response to IBA was observed fordone 2 with the greatest rooting occurring for the nontreated cuttings (89%). Softwood cuttings of Q. canbyi responded quadratically to IBA treatment, with maximum rooting of 33% noted for cuttings treated with 1500 ppm IBA.


2009 ◽  
Vol 27 (1) ◽  
pp. 12-16
Author(s):  
John M. Wooldridge ◽  
Frank A. Blazich ◽  
Stuart L. Warren

Abstract Two experiments, one utilizing softwood cuttings and the other semi-hardwood cuttings, were conducted to investigate the influence of growth stage and auxin treatment on rooting four related clones of eastern redbud (Cercis canadensis L.). The four clones were ‘Flame’ (C. canadensis L. ‘Flame’), dwarf white, and two selections (NC99-6-1 and NC99-6-2) of an F1 generation derived from a cross of ‘Flame’ and dwarf white. At each growth stage, rooting responses of the clones varied and were influenced greatly by auxin treatment, demonstrating the variable rooting potential of the genotypes. In both studies, ‘Flame’ rooted well [63% rooting in the softwood study when treated with the potassium (K) salt (K-salt) of indolebutyric acid (IBA) at 5000 mg·L−1 (ppm), 83% rooting when treated in the semi-hardwood study with K-IBA at 10,000 mg·L−1], indicating stem cuttings may be a commercially feasible means of propagation for ‘Flame.’ Softwood cuttings of dwarf white (46%) and NC99-6-2 (75%) rooted best when treated with K-IBA at 15,000 mg·L−1, whereas softwood cuttings of NC99-6-1 rooted best (46%) when treated with K-IBA at 10,000 mg·L−1. Dwarf white and the F1s rooted poorly in the semi-hardwood study.


1998 ◽  
Vol 16 (4) ◽  
pp. 212-214
Author(s):  
Jason J. Griffin ◽  
Frank A. Blazich ◽  
Thomas G. Ranney

Abstract Stem cuttings of Thuja L. x ‘Green Giant’ (‘Green Giant’ arborvitae), consisting of 22 cm (8.6 in) terminals (tips of first-order laterals) or 20 cm (7.8 in) laterals (side shoots removed from those portions of terminal cuttings inserted into the rooting medium), were collected on three dates associated with specific stock plant growth stages (softwood, semi-hardwood, and hardwood) and treated with indolebutyric acid (IBA) in 50% isopropanol ranging from 0 to 9000 ppm (0.9%). Regardless of cutting type or auxin treatment, cuttings rooted in high percentages at each growth stage. Overall rooting for softwood, semi-hardwood, and hardwood, cuttings was 85%, 86%, and 96%, respectively. Semi-hardwood cuttings were the only cuttings in which percent rooting was affected by IBA treatment or cutting type, with lateral cuttings rooting in higher percentages than terminal cuttings (92% vs. 79%, respectively), while IBA concentrations of 3000 (0.3%) or 6000 ppm (0.6%) proved optimal for both cutting types. Mean root number and mean root length varied by growth stage, cutting type, and IBA concentration. In general, rooting percentages and root length were optimized at the hardwood stage with either cutting type treated with 3000, 6000 or 9000 ppm (0.3%, 0.6% or 0.9%) IBA, resulting in >10 roots per cutting.


2008 ◽  
Vol 26 (1) ◽  
pp. 1-3
Author(s):  
Jason J. Griffin

Abstract Viburnum rufidulum Raf. (southern or rusty blackhaw) has potential to be a popular landscape plant as it is an attractive large shrub tolerant of many common landscape stresses. However, propagation difficulties have thus far limited wide scale use. Therefore, the influence of IBA formulation and concentration on adventitious rooting of stem cuttings of southern blackhaw taken at different stock plant growth stages throughout the year were investigated. Liquid formulations of the potassium salt (K-salt) of indolebutyric acid (K-IBA) at 0, 3000, 6000, or 9000 ppm (0, 0.3, 0.6, or 0.9%) as well as talc formulations of IBA at 1000, 3000, or 8000 ppm (0.1, 0.3 or 0.8%) were utilized. Talc formulations failed to stimulate rooting regardless of concentration or growth stage. A quick-dip of K-IBA increased rooting percentage at all growth stages. Softwood and hardwood cuttings had the highest rooting percentages. Hardwood cuttings treated with 6000 ppm (0.6%) or 9000 ppm (0.9%) rooted 90 and 100%, respectively. Softwood cuttings treated with 6000 ppm (0.6%) rooted 87%. K-IBA improved root number per rooted cutting for softwood cuttings, whereas root length was unaffected by K-IBA at any growth stage.


HortScience ◽  
1994 ◽  
Vol 29 (12) ◽  
pp. 1532-1535 ◽  
Author(s):  
Laura G. Jull ◽  
Stuart L. Warren ◽  
Frank A. Blazich

Stem cuttings of `Yoshino' Japanese cedar [Cryptomeria japonica (L.f.) D. Don `Yoshino'], consisting of tips (terminal 20 cm) of first-order laterals, distal halves (terminal 10 cm) of tips of first-order laterals, and proximal halves (basal 10 cm) of tips of first-order laterals, or tips (terminal 10 cm) of second-order laterals, were taken on four dates that represented four growth stages (softwood, semi-hardwood, hardwood, and pre-budbreak). The cuttings were treated with 0, 3000, 6000, or 9000 mg IBA/liter. Branch order affected all rooting measurements at each growth stage. Regardless of growth stage, tips of and proximal halves of first-order laterals containing lignified wood had the highest percent rooting, root count, total root length, root area, and root dry weight. Hardwood tips of and semi-hardwood proximal halves of first-order laterals exhibited the highest overall rooting (87%), followed by softwood proximal halves of first-order laterals (78%). Rooting of distal halves of first-order laterals and tips of second-order laterals never exceeded 55% and 34%, respectively, at any growth stage. IBA treatment influenced percent rooting, root count, total root length, root area, and root dry weight of semi-hardwood, hardwood, and pre-budbreak cuttings, except for root dry weight of semi-hardwood cuttings. IBA had no affect on softwood cuttings. Chemical name used: 1H-indole-3-butyric acid (IBA).


2021 ◽  
Vol 4 (2) ◽  
pp. 291
Author(s):  
Indri Elang Mayanti ◽  
Basir Achmad

The areas of tropical forests in Indonesia always decrease every year, so that efforts are needed to cultivate plants efficiently for the supply of seedlings. Sungkai (Peronema canescens) has good quality wood that can be used for various purposes, either for construction, furniture, plywood or the leaves can be used for medicine. The purpose of this study was to analyze the growth of stem cuttings of sungkai based on the number of latent buds by calculating the root dry weight, bud dry weight, and total dry weight. This study used a factorial complete randomized design. There were two factors studied, namely the number of buds consisting of 2 levels: 2 buds and 1 bud, and the stem split factor. Each treatment was replicated 3 times and each experiment unit used 5 cuttings. The growth of sungkai cuttings in the treatment of two buds had a total dry weight of 170.04 grams, while the treatment of one bud had a total dry weight of 98.30 grams. The results of this study indicated that the more the number of latent buds, the more root and bud growth of cuttings.Keywords: Coppice; Latent buds; Cuttings; Sungkai


Author(s):  
Khalid S. Alshallash Khalid S. Alshallash

In four glasshouse experiments, the effectiveness of the adult green dock beetle Gastrophysa viridula (Coleoptera: Chrysomelidae), at the effective number of applied individuals, for use as a biological control agent of curled dock, Rumex crispus (Polygonaceae) were studied. The feeding of the beetle was investigated at four different numbers of beetle (0, 1, 2, 3) and at four seedling growth stages of the plant, defined by the average of leaf area per plant (1-1.22 , 2-4.45, 3-11.56, and 4-71.52 cm2/plant). Grazing by one, two or three dock beetles did not result in a significant reduction in dock dry weight or shoot numbers at the youngest growth stage. However, both at later seedling growth stages were significantly affected (P ? 0.0001), at any beetles number. The increase of beetle numbers caused nonsignificant increased effect, in some trials, confirming the impact of a single beetle. Three months after beetle grazing, dock seedlings of first, second and third growth stages were not able to regrow, however, some plants at the 4th growth stage, re-emerged. This suggested that the highest effect of beetle's feeding occurs on the early seedling stages. Statistical analysis showed a positive correlation (0.77) between dry weight and shoot number at all the four seedling growth stages, thus confirming the impact of the beetle on both the dry weight and shoot numbers. Combining beetle grazing with other control methods at older dock seedling stages could, therefore, provide better suppression


Weed Science ◽  
2004 ◽  
Vol 52 (1) ◽  
pp. 178-182 ◽  
Author(s):  
Donnie K. Miller ◽  
Robert G. Downer ◽  
B. Roger Leonard ◽  
E. Merritt Holman ◽  
Steve T. Kelly

Field research was conducted in 1999 and 2000 to determine the effect of reduced glyphosate rates on growth and yield of nonglyphosate-resistant cotton. Rates of 9, 18, 35, 70, 140, and 280 g ha−1, representing 0.008, 0.016, 0.031 0.063, 0.125, and 0.25, respectively, of the maximum use rate per application (1,120 g ha−1), were applied to cotton at the two-, five-, or nine-node growth stage. On the basis of visual injury estimates, cotton was more tolerant to glyphosate at the nine-node than at earlier growth stages. Plant dry weight was reduced with 70 g ha−1of glyphosate or higher, when applied at the two- and five-node growth stages in two of three experiments. Dry weight was not affected by glyphosate at the nine-node stage. Plant height also was unaffected by glyphosate rates below 70 g ha−1, but height reduction was noted for all growth stages by experiment combinations, with the exception of the nine-node application for both experiments in 2000, with herbicide rates of 70 g ha−1or higher. Cotton maturity delay, as noted by an increase in node above white flower number, was observed only at the highest glyphosate rate applied to two- and five-node cotton in one of three experiments. Percent open boll data analysis indicated a decreased opportunity of observing an open boll with increasing glyphosate rate, and this effect was greater at the five-node compared with the two- and nine-node stages in two of three experiments. Seedcotton yield after all glyphosate applications was equivalent to that for the nontreated control.


2003 ◽  
Vol 21 (2) ◽  
pp. 61-63
Author(s):  
Peter J. Conden ◽  
Frank A. Blazich

Abstract Two experiments were conducted to develop a protocol for propagation of Castanopsis sclerophylla (Lindley & Paxton) Schottky by stem cuttings. In the first experiment completed in 2000, stem cuttings were taken on three dates representing three growth stages (softwood, semi-hardwood, or hardwood). Semi-hardwood and hardwood cuttings were treated with 0, 2500 (0.25%), 5000 (0.5%), 7500 (0.75%), or 10,000 (1.0%) ppm of the free acid of indolebutyric acid (IBA) dissolved in 50% isopropyl alcohol, whereas softwood cuttings were treated with the same concentrations of the potassium (K) salt of IBA (K-IBA) dissolved in distilled water. Cuttings were placed in a raised greenhouse bench and rooted under intermittent mist. Cuttings taken at the semi-hardwood and hardwood stages began to drop their leaves after approximately 2 weeks and the majority eventually died, with negligible rooting of surviving cuttings. The response of the softwood cuttings to K-IBA was quadratic with the greatest rooting (63%) at 7500 ppm K-IBA. Seventy softwood cuttings that rooted were potted for observance of overwinter survival. Of these, 90% produced a flush of growth during the summer, with 80% overwinter survival. In the second experiment conducted in 2001, semi-hardwood cuttings were taken and treated with 0, 2500 (0.25%), 5000 (0.5%), 7500 (0.75%), or 10,000 (1.0%) ppm K-IBA and placed in a raised greenhouse bench with intermittent mist for rooting as in the first experiment. Results were similar to the semi-hardwood cuttings treated in the first experiment with the free acid of IBA, indicating semi-hardwood cuttings do not root and that the auxin and/or isopropyl alcohol were not toxic to the cuttings.


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