scholarly journals Polyaniline modified silica gel coupled with green solvent as eco favourable mobile phase in thin layer chromatographic analysis of organic dyes

2021 ◽  
Vol 4 (1) ◽  
pp. 41
Author(s):  
Mahfoozurrahman Khan ◽  
Ali Mohammad ◽  
Qasim Ullah ◽  
Faiz Mohammad
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Silica Gel ◽  
Mobile Phase ◽  
Organic Dyes ◽  
Thymol Blue ◽  
Transmission Electron Micrograph ◽  
Chromatographic System ◽  
Modified Silica ◽  
Modified Silica Gel

This article studies a new green eco-friendly TLC (thin layer chromatography) using silica gel and polyaniline modified silica gel as stationary phase in combination with ethyl acetate (EA), n-butyl acetate (BA) and butane-1-ol (BO) solutions as mobile phase for the comparative study of migration behaviour of organic dyes to identify the most suitable thin layer chromatographic system for the resolution of co-existing dyes. Better separation efficiency was observed by modifying silica gel with polyaniline as compared to pure silica stationary phase. Densitogrpahic presentation of separations achieved on polyaniline modified silica gel Pani@SG-EB1 was also presented. The thin layer chromatographic system comprising of polyaniline modified silica gel Pani@SG-EB1 as stationary phase and n-butyl acetate:DDW, 5:5 as green mobile phase was observed to be the most favourable for the separation of various combinations of three or four-component mixtures of organic dyes viz. methyl thymol blue, tartrazine, carmoisine, rose bengal, amidoblack 10B, bromopyrogallol red and 4-nitrobenzene dizonium tetrafluoroborate. The effect of presence of cations and anions on separation trend was also examined and the limits of detection of the separated organic dyes were estimated. Fourier transform infrared spectroscopy (FTIR), x-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron micrograph (TEM) studies were undertaken to characterize silica gel and modified silica gel (stationary phase). The developed method has been successfully applied for the identification of carmoisine in Solvin cold DS syrup and tartrazine in MefastTM syrup.

Radial multi-stationary phase thin-layer chromatography for the field-ready fingerprinting of herbal materials

2019 ◽  
Vol 11 (43) ◽  
pp. 5511-5520 ◽  
Author(s):  
Sarah May Sibug-Torres ◽  
Isagani D. Padolina ◽  
Erwin P. Enriquez
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Stationary Phases ◽  
Modified Silica ◽  
Modified Silica Gel ◽  
Field Screening ◽  
Plate Design ◽  
Layer Chromatography

A novel TLC plate design that features multiple screen printed phenyl- and octyl-modified silica gel stationary phases configured as radial sectors was developed for the in-field screening of herbal materials.

Tetraazacalix[2]arene[2]triazine modified silica gel: A novel multi-interaction stationary phase for mixed-mode chromatography

2012 ◽  
Vol 1251 ◽  
pp. 74-81 ◽  
Author(s):  
Wenjie Zhao ◽  
Wenjing Wang ◽  
Hong Chang ◽  
Shiwei Cui ◽  
Kai Hu ◽  
...  
Keyword(s):  
Stationary Phase ◽  
Silica Gel ◽  
Mixed Mode ◽  
Modified Silica ◽  
Modified Silica Gel

scholarly journals 4-Chloro-6-pyrimidinylferrocene modified silica gel: A novel multiple-function stationary phase for mixed-mode chromatography

Talanta ◽  
2016 ◽  
Vol 153 ◽  
pp. 8-16 ◽  
Author(s):  
Lijun Qiao ◽  
Xiaohua Zhou ◽  
Yanhao Zhang ◽  
Ajuan Yu ◽  
Kai Hu ◽  
...  
Keyword(s):  
Stationary Phase ◽  
Silica Gel ◽  
Mixed Mode ◽  
Modified Silica ◽  
Modified Silica Gel ◽  
Multiple Function

Calixarene ionic liquid modified silica gel: A novel stationary phase for mixed-mode chromatography

Talanta ◽  
2016 ◽  
Vol 152 ◽  
pp. 392-400 ◽  
Author(s):  
Kai Hu ◽  
Wenfen Zhang ◽  
Huaixia Yang ◽  
Yongxia Cui ◽  
Jingya Zhang ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Stationary Phase ◽  
Silica Gel ◽  
Mixed Mode ◽  
Modified Silica ◽  
Modified Silica Gel

scholarly journals Estimating Limits of Detection and Quantification of Ibuprofen by TLC-Densitometry at Different Chromatographic Conditions

Processes ◽  
2020 ◽  
Vol 8 (8) ◽  
pp. 919 ◽  
Author(s):  
Josef Jampilek ◽  
Malgorzata Dolowy ◽  
Alina Pyka-Pajak
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Mobile Phase ◽  
Modified Silica Gel ◽  
Limits Of Detection ◽  
Wide Range ◽  
The Impact ◽  
Layer Chromatography ◽  
Detection And Quantification

Ibuprofen is one of the best-known nonsteroidal anti-inflammatory and analgesic drugs. Following the previous work, the current study is focused on estimating the effect of different chromatographic conditions on the sensitivity of thin-layer chromatography in combination with UV densitometry, i.e., the detection and quantification of ibuprofen in a wide range of its concentrations including the lowest limits of detection (LOD) and quantification (LOQ). For this purpose, a reliable and easy-to-use calculation procedure for LOD and LOQ determination is presented in this work. In addition, the impact of type plates and mobile phase composition on the LOD and LOQ, respectively, of this active substance is accurately described. The results of detection and the quantification level of ibuprofen obtained under applied chromatographic conditions confirmed the utility of silica gel plates as well as silica gel bonded phases (i.e., reversed-phase (RP) plates) in the thin-layer chromatography (TLC)-densitometric analysis of ibuprofen at a low level, i.e., from several nanograms (below one microgram) to a few micrograms/spot. Among all chromatographic systems tested, the best are those consisting of silica gel 60F254 plates with concentrating zone (1.05583) and the mixture of n-hexane:ethyl acetate:glacial acetic acid in ratio 36:12:2 (v/v/v) as well as RP plates, i.e., RP-18F254 aluminum plates developed with methanol:water in volume composition 40:10 (v/v). These chromatographic systems allowed quantifying ibuprofen in the amount of 0.229 µg/spot and 0.228 µg/spot, which is less than 1 µg/spot. It can be stated that different chromatographic systems in combination with UV densitometric scanning at 224 nm proposed in this work can be successfully applied for the cost-effective and sensitive determination of ibuprofen as a widely used drug component as well as a residual in domestic wastewater. It was found that the modification of silica gel as well as the layer thickness of unmodified or modified silica gel 60 can influence the quality of chromatograms and the detection/quantification of ibuprofen in both normal phase (NP) and RP systems. Therefore, to obtain the best possible LOD and LOQ values of ibuprofen with precoated layers, suitable mobile phase and chromatographic plates are required.

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