scholarly journals Studies on structural elucidation of delphinium alkaloids by using LC-ESI-MS technique.

2015 ◽  
Vol 11 (3) ◽  
pp. 3399-3411
Author(s):  
Bilal Ahmad Dar ◽  
Mushtaq Ahmad Qurishi ◽  
Aijaz Hussain Kanth
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Negative Ion ◽  
Tentative Identification ◽  
Esi Ms ◽  
Short Run ◽  
Positive Mode ◽  
Fragmentation Patterns ◽  
Negative Ion Mode ◽  
Mode Response

A rapid,sensitive and specific liquid chromatography-electron spray-mass spectrometry (LC-ESI-MS) method to identify the different diester-diterpenoid and monoester type alkaloids from delphinium cashmerianum Collected from Sopi Kargil. Chromatographic separation were achieved on c-18 coloumn and peaks determined by mass spectrometry in positive and negative modes.The validated method led to tentative identification of eight alkaloids on the basis of their retention times and fragmentation patterns. Results showed that the positive mode response was much higher than the negative ion mode .chromatographic conditions were optimized to obtain high resolution and short run time.

Structural Characterization of Nitrated 2′-Hydroxychalcones by Electrospray Ionization Tandem Mass Spectrometry

2009 ◽  
Vol 15 (5) ◽  
pp. 605-616 ◽  
Author(s):  
Ana I.R.N.A. Barros ◽  
Fernando M. Nunes ◽  
Cristina Barros ◽  
Artur M.S. Silva ◽  
M. Rosário M. Domingues
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Electrospray Ionization ◽  
Structural Information ◽  
Tandem Mass ◽  
Substitution Pattern ◽  
Esi Ms ◽  
Positive Mode ◽  
Fragmentation Patterns ◽  
Product Ion Spectra

Isomeric 2′-hydroxychalcones bearing nitro and methoxy groups in different positions of their skeleton were analyzed by tandem mass spectrometry (MS/MS) with electrospray ionization (ESI), in positive mode. Collision-induced dissociation of the protonated molecules, [M + H]+, formed under electrospray conditions were studied and it was found that the product ion spectra of these chalcones presented different fragmentation patterns depending on the position of the substituents on the molecule. The product ion spectra (ESI-MS/MS) of the B ring ortho-nitro substituted 2′-hydroxychalcone and of the 4′-methoxychalcones showed loss of OH•, 2OH• and combined losses of OH• and H2O. These fragment ions were absent in the spectra of the respective meta- and para isomers. The observed differences in the product ion spectra of these nitrochalcones allowed identification of the o-nitro derivatives. Distinction between the meta- and para-derivatives was not achieved. Chalcones bearing 6′-methoxy substituents showed distinct fragmentation from the one observed for their isomers, 4′-methoxychalcones, since they present only one fragment ion, a typical (0,αA – H)+ and, therefore, do not allow detailed structural information to be obtained, nor to differentiate between the o-, m- or p-nitro isomers. Overall, it was found that small changes in the substitution pattern of chalcones change their fragmentation considerably in the ESI-MS/MS, and that these features permit the differentiation of specific isomers of these 2′-hydroxynitrochalcones.

scholarly journals Isolation and Identification of Chemical Constituents from Zhideke Granules by Ultra-Performance Liquid Chromatography Coupled with Mass Spectrometry

2020 ◽  
Vol 2020 ◽  
pp. 1-16
Author(s):  
Guangqiang Huang ◽  
Jie Liang ◽  
Xiaosi Chen ◽  
Jing Lin ◽  
Jinyu Wei ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Chemical Constituents ◽  
Relevant Literature ◽  
Ultra Performance Liquid Chromatography ◽  
Negative Ion ◽  
Volatile Components ◽  
Isolation And Identification ◽  
Fragmentation Patterns ◽  
Nitrogen Containing Compounds

Chemical constituents from Zhideke granules were rapidly isolated and identified by ultra-performance liquid chromatography (UPLC) coupled with hybrid quadrupole-orbitrap mass spectrometry (MS) in positive and negative ion modes using both full scan and two-stage threshold-triggered mass modes. The secondary fragment ion information of the target compound was selected and compared with the compound reported in databases and related literatures to further confirm the possible compounds. A total of 47 chemical constituents were identified from the ethyl acetate extract of Zhideke granules, including 21 flavonoids and glycosides, 9 organic acids, 4 volatile components, 3 nitrogen-containing compounds, and 10 other compounds according to the fragmentation patterns, relevant literature, and MS data. The result provides a new method for the analysis of chemical constituents of Zhideke granules which laid the foundation for quality control and the study of pharmacodynamic materials of Zhideke granules.

scholarly journals Flavonol glycosides found in hydroethanolic extracts from Tilia cordata, a species utilized as anxiolytics

2013 ◽  
Vol 15 (2) ◽  
pp. 217-224 ◽  
Author(s):  
G. Negri ◽  
D. Santi ◽  
R. Tabach
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Chemical Composition ◽  
Folk Medicine ◽  
Anxiolytic Activity ◽  
Flavonol Glycosides ◽  
Negative Ion ◽  
Tilia Cordata ◽  
Negative Ion Mode ◽  
Hydroethanolic Extract

Tilia species, among which is Tilia cordata Mill. (Tiliaceae), have been used in folk medicine as anxiolytic. The hydroethanolic extract was analyzed by using liquid chromatography with mass spectrometry HPLC-DAD-ESI-MS/MS in negative ion mode, and its chemical composition was compared to flavonoids reported as anxiolytics. The major flavonoids found were: quercetin-3,7-di-O-rhamnoside, kaempferol-3,7-di-O-rhamnoside and kaempferol 3-O-(6"-p-coumaroyl glucoside) or tiliroside. The anxiolytic activity of the genus Tilia has been attributed to the presence of quercetin and kaempferol derivatives, while the anxiolytic activity of T. americana var. Mexicana was attributed to tiliroside, which was also found among the major constituents of this species.

Comparative Analysis of the Chemical Consistency Between the Traditional and Mixed Decoction of Maimendong Decoction by Ultra-Performance Liquid Chromatography Coupled to Quadrupole with Time-of-Flight Mass Spectrometry (UPLC–QTOF-MS)-Based Chemical Profiling Approach

2020 ◽  
Vol 58 (6) ◽  
pp. 549-561 ◽  
Author(s):  
Lan Yang ◽  
Zhenhua Zhu ◽  
Zhonghua Qi ◽  
Xinsheng Fan ◽  
Dawei Qian ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Time Of Flight ◽  
Ultra Performance Liquid Chromatography ◽  
Negative Ion ◽  
Chemical Profiling ◽  
Flight Mass Spectrometry ◽  
Traditional Preparation ◽  
Ginsenoside Rg5 ◽  
Negative Ion Mode

Abstract Take Maimendong Decoction (MMDD), one of the Chinese classic herbal formulas, as an object to evaluate the chemical consistency between traditional decoction and mixed decoction. The ultra-performance liquid chromatography coupled to quadrupole with time-of-flight mass spectrometry-based chemical profiling approach has been utilized. A total of 48 major peaks are detected from these two decoctions under the present chromatographic and mass spectrometry conditions. The results of negative ion mode show nine significant inconsistencies. Liquiritin, ginsenoside Ro and ginsenoside Rg5/Rk1 are detected with higher intensity in traditional preparation sample than the mixed decoction, while licoisoflavone A is higher in mixed decoction samples than the traditional one. The mechanisms involved in the chemical changes were assumed to be anti-inflammation, anti-oxidative stress and so on, suggesting these two different preparation approaches of MMDD may lead to a possibility of discrepant clinical outcomes.

Characterization of organosolv switchgrass lignin by using high performance liquid chromatography/high resolution tandem mass spectrometry using hydroxide-doped negative-ion mode electrospray ionization

2014 ◽  
Vol 16 (5) ◽  
pp. 2713-2727 ◽  
Author(s):  
Tiffany M. Jarrell ◽  
Christopher L. Marcum ◽  
Huaming Sheng ◽  
Benjamin C. Owen ◽  
C. J. O'Lenick ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Resolution ◽  
High Performance ◽  
Negative Ion ◽  
Tandem Mass ◽  
Organosolv Lignin ◽  
Negative Ion Mode

Method for identification of compounds in organosolv lignin.

Sign in / Sign up

Export Citation Format

Share Document