Assessment of the Effect of C-Peptide Level on Na-K ATPase Activity In Individuals with Type II Diabetic Peripheral Neuropathy

2021 ◽  
Vol 53 (03) ◽  
pp. 213-219
Author(s):  
Eman H Al-Rikabi ◽  
Keyword(s):  
Enzyme Activity ◽  
Diabetic Neuropathy ◽  
Atpase Activity ◽  
Negative Correlation ◽  
Clinical Symptoms ◽  
The Other ◽  
C Peptide ◽  
Other Hand ◽  
Peptide Level

Background: Both Type 1 and Type 2 diabetes can cause neuropathy, which is a frequent and severe consequence. C-peptide depletion may be partly related to in the occurrence of certain diabetic complications. It has been demonstrated that even a little amount of residual C-peptide has a considerable metabolic advantage. Objective: The study’s objective was to predict the relation of plasma C-peptide levels in patients with diabetic neuropathy, and its effect on Na-K ATPase activity. Design and Methods: In this case-control study, 150 individuals have been included: 80 patients with diabetic neuropathy, 40 diabetics without neuropathy and 30 non-diabetic subjects as a control. Patients in the first group were carefully chosen based on their clinical symptoms and nerve conduction studies results. The assessment of plasma C-peptide was done by ELISA, Na-K ATPase enzyme activity by spectrophotometer, and HbA1C by HPLC. Results: Mean plasma C-peptide level and Erythrocyte Na-K ATPase activity were substantially lower in neuropathy type 2 DM patients compared to diabetes without neuropathy and control (p= 0.002, 0.000 respectively). The negative correlation between C-peptide with HbA1c, and diabetes period were all negligible (p= 0.447,0.098), Even though there was a notable negative correlation with age (p= 0.03). On the other hand, the relationship linking C-peptide and Na-K ATPase enzyme activity was shown to be insignificant (p=0.69). Conclusions: Diabetic neuropathy is related to a low C-peptide level. The association between C-peptide and Na-K ATPase enzyme activity, on the other hand, was shown to be insignificant. C-peptide HbA1c, and duration of diabetes all had minor negative associations.

TYPE 2 DIABETES MELLITUS REMISSION AND ITS PREDICTION AFTER TWO-STAGE SURGICAL TREATMENT OF PATIENTS WITH MORBID OBESITY

2019 ◽  
Vol 72 (5) ◽  
pp. 739-743
Author(s):  
Oleksandr Yu. Ioffe ◽  
Mykola S. Kryvopustov ◽  
Yuri A. Dibrova ◽  
Yuri P. Tsiura
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Morbid Obesity ◽  
Weight Loss ◽  
Type 2 Diabetes Mellitus ◽  
Surgical Treatment ◽  
Two Stage ◽  
C Peptide ◽  
Peptide Level

Introduction: Morbid obesity (MO) has a significant impact on mortality, health and quality of life of patients. Type 2 diabetes mellitus (T2DM) is a common comorbidity in patients with MO. The aim is to study T2DM remission and to develop a prediction model for T2DM remission after two-stage surgical treatment of patients with MO. Materials and methods: The study included 97 patients with MO. The mean BMI was 68.08 (95% CI: 66.45 - 69.71) kg/m2. 70 (72,2%) patients with MO were diagnosed with T2DM. The first stage of treatment for the main group (n=60) included the IGB placement, for the control group (n=37) - conservative therapy. In the second stage of treatment the patients underwent bariatric surgery. The study addresses such indicators as BMI, percentage of weight loss, percentage of excess weight loss, ASA physical status class, fasting glucose level, HbA1c, C-peptide. Results: Two-stage treatment of morbidly obese patients with T2DM promotes complete T2DM remission in 68.1% of patients. The risk prediction model for failure to achieve complete T2DM remission 12 months after LRYGB based on a baseline C-peptide level has a high predictive value, AUC = 0.84 (95% CI: 0.69-0.93), OR = 0.23 ( 95% CI: 0.08-0.67). Conclusions: Two-stage treatment of patients with MO promotes improvement of carbohydrate metabolism indicators. With a C-peptide level > 3.7 ng/ml, prediction of complete T2DM remission 12 months after Laparoscopic Roux-en-Y Gastric Bypass is favorable.

scholarly journals Basal C-peptide Level as a Surrogate Marker of Subclinical Atherosclerosis in Type 2 Diabetic Patients

2011 ◽  
Vol 35 (1) ◽  
pp. 41 ◽  
Author(s):  
Sung-Tae Kim ◽  
Byung-Joon Kim ◽  
Dong-Mee Lim ◽  
In-Geol Song ◽  
Jang-Han Jung ◽  
...  
Keyword(s):  
Subclinical Atherosclerosis ◽  
Surrogate Marker ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
C Peptide ◽  
Peptide Level ◽  
Type 2 Diabetic

Trypanosoma cruzi Epimastigotes Express the Ouabain-and Vanadate-Sensitive (Na++K+)ATPase Activity

1998 ◽  
Vol 53 (11-12) ◽  
pp. 1049-1054 ◽  
Author(s):  
Celso Caruso-Neves ◽  
Marcelo Einicker-Lamas ◽  
Carlos Chagas ◽  
Mecia Maria Oliveira ◽  
Adalberto Vieyra ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Atpase Activity ◽  
The Other ◽  
Other Hand ◽  
P Type

Abstract The presence of (Na++K+)ATPase activity in CL14 clone and NIH NTY strain of Trypano­soma cruzi epimastigotes is demonstrated. A Na+ plus K+ stimulated ATPase activity is found in both strains. The optimal Na+/K+ ratio is 5:1 and 9:1 in CL14 clone and NIH NTY strain, respectively. In both strains, vanadate completely inhibits the ouabain-sensitive ATPase activ­ity indicating that it belongs to the P-type (E 1/E2) family of ion-transporting ATPases. The I50 for vanadate is 0.66 ± 0.04 and 0.04 ± 0.02 μᴍ in CL14 clone and NIH NTY strain, respectively. These data indicate that both strains of T. cruzi epimastigotes express the oua­ bain-and vanadate-sensitive (Na++K+)ATPase activity. On the other hand, the discrepancy between the parameters analyzed for the inhibitors suggests that they express different iso­ forms of this enzyme.

scholarly journals Effect of phytoplasma infection on the activity of peroxidase, β-1,3 glucanase and chitinase in corn plants

2011 ◽  
Vol 37 (4) ◽  
pp. 194-198
Author(s):  
Ana Carolina Bruno Junqueira ◽  
Ivan Paulo Bedendo ◽  
Sérgio Florentino Pascholati
Keyword(s):  
Enzyme Activity ◽  
Plant Resistance ◽  
The Other ◽  
Dalbulus Maidis ◽  
Other Hand ◽  
Phytoplasma Infection ◽  
Corn Plants ◽  
Maize Bushy Stunt Phytoplasma ◽  
Host Metabolism ◽  
Corn Hybrid

In the present work we studied the effect of inoculating corn plants with the maize bushy stunt phytoplasma on the activity of the enzymes peroxidase, β-1,3 glucanase and chitinase. The experiments were carried out inside a greenhouse. Plants of a resistant and a susceptible corn hybrid were inoculated by using infective Dalbulus maidis leafhoppers 10 days after sowing. When symptoms started to appear, leaf samples were collected at different periods to quantify enzyme activity. The results showed an increase in the activity of the three enzymes in inoculated plants of both hybrids. In general, the values observed for the level of the different enzymes were higher in the susceptible hybrid when compared to the resistant one. Thus, the increases in peroxidase, β-1,3 glucanase and chitinase levels in inoculated plants are evidence of changes in the host metabolism caused by the phytoplasma. On the other hand, since the increases could not be correlated with plant resistance further studies are needed to explain such changes.

All idempotent and regular elements in the monoid of generalized hypersubstitutions for algebraic systems of type (2; 2)

2019 ◽  
pp. 2150015
Author(s):  
D. Phusanga ◽  
J. Joomwong ◽  
S. Jino ◽  
J. Koppitz
Keyword(s):  
The Other ◽  
Algebraic Systems ◽  
General Algebra ◽  
Regular Elements ◽  
Other Hand

There are two different concepts for hypersubstitutions for algebraic systems [K. Denecke and D. Phusanga, Hyperformulas and solid algebraic systems, Studia Logica 90(2) (2008) 263–286; J. Koppitz and D. Phusanga, The monoid of hypersubstitutions for algebraic systems, J. Announcements Union Sci. Sliven 33(1) (2018) 120–127]. In this paper, we follow the more natural and practicable one given in [J. Koppitz and D. Phusanga, The monoid of hypersubstitutions for algebraic systems, J. Announcements Union Sci. Sliven 33(1) (2018) 120–127]. On the other hand, in [S. Leeratanavalee and K. Denecke, Generalized hypersubstitutions and strongly solid varieties, General Algebra and Applications[Formula: see text] Proc. of 59th Workshop on General Algebra[Formula: see text] 15th Conf. for Young Algebraists Potsdam 2000 (Shaker Verlag, 2000), pp. 135–145], the concept of the monoid of generalized hypersubstitutions was introduced. Following both ideas, one obtains the concept of a monoid of generalized hypersubstitutions for algebraic systems in a canonical way. The purpose of this paper is the study of the monoid of generalized hypersubstitutions for algebraic systems. We characterize the idempotent as well as regular elements in this monoid.

scholarly journals THE HISTOCHEMICAL DEMONSTRATION OF NUCLEASE ACTIVITY WITH FILMS OF SOLUBLE RIBONUCLEIC ACID AND POLYADENYLIC ACID

1972 ◽  
Vol 20 (5) ◽  
pp. 350-357 ◽  
Author(s):  
R. DAOUST ◽  
R. MORAIS
Keyword(s):  
Enzyme Activity ◽  
Ribonucleic Acid ◽  
Nuclease Activity ◽  
Histochemical Demonstration ◽  
The Other ◽  
Rat Tissues ◽  
Other Hand ◽  
Polyadenylic Acid ◽  
Normal Rat

Films of soluble ribonucleic acid (sRNA) and polyadenylic acid (poly-A) were used to investigate the distribution of nuclease activity in normal rat tissues. The reactions obtained with films of sRNA were similar to those previously observed with standard RNA and both substrates apparently reveal the same group of nucleases. On the other hand, the distribution of enzyme activity shown by films of poly-A differed markedly from that observed with RNA films, and it appears that films of poly-A demonstrate a different group of nucleases.

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