An Assessment of Prominent Risks Factors Behind the Development of COVID-19 Pandemic Digital Eye Strain in context with the Online Class Room

The COVID-19 outbreak has destructively affected the education system over the world and brought about prominent alterations in the traditional systems of teaching and learning. In order to flatten the curve of disease transmission, several countries have resorted to online classes for students. Apart from the benefits of this strategy, there exists a chance of developing ocular complications in students those are continuously viewed the digital screen without any break. T-test and frequency analysis was used to find out the link between the digital electronic gadgets and eye strains. SPSS version 24.0.0 was used for all the statistical analyses. Here we assessed the various prominent factors behind the development of complications in eyes instigated by the electronic gadgets with special inference on the type of electronic gadgets, time spend, distance, type of usage, kind of posture, light condition, stress, and kind of symptoms towards digital eye strains. One of the major fact that has been emerged in this investigation is that the usage of electronic gadgets for online classes purposes have greatly increased than the other usage. The use of electronic gadgets for work and educational purposes has been recognized as a normal activity among individuals. With the extensive prevalence of developing digital eye strain in the students, it is paramount that the results of this investigation can be used for research purposes as strong research evidence to prevent the complications allied with eyes.

The Study of Biochemical Markers in Chronic Renal Failure Patients in Pre- and Post-Haemodialysis

Background: The current research is designed to investigate alterations in lipid peroxidation (malondialdehyde - MDA) and renal markers (urea and creatinine) in patients of chronic renal failure (CRF) as compared to the control group. Method: The study included 55 subjects, who were separated into two groups: control group, which included 15 healthy members with no history of systematic illness; and patients group, which included 40 patients with CRF divided into four groups “Non: CRF patients without any accompanied disease, DM: CRF patients with diabetes mellitus, HT: CRF patients with hypertension, and HT + DM: CRF patients with diabetes mellitus and hypertension”. Results: The findings reveal that there is a notable increase in serum concentration of MDA, urea, and creatinine, in patients group as compared to the control group. Conclusion: In all chronic renal failure patients with or without any accompanying disease, lipid peroxidation is present in pre- and post-haemodialysis patients as well as patients with CRF have high levels of urea and creatinine compared with healthy groups.

Insecticides Susceptibility Status of Malaria Vectors in a High Malaria Endemic Tribal District Gadchiroli (Maharashtra) of India

Background and Objective: The current study was undertaken to determine insecticide susceptibility of malaria vectors in various villages of high malaria endemic PHCs of Gadchiroli district of Maharashtra. Methods: Adult malaria vectors were collected from the human dwellings/ cattle sheds of 156 villages of 18 malaria endemic PHCs. Susceptibility tests were carried out for different insecticides against An. culicifacies and An. fluviatilis mosquitoes as per the World Health Organization (WHO) procedure. Cone bioassays were also done to assess the quality and efficacy of indoor residual spray. Results:An. fluviatilis could be collected from 23 villages only and all the populations were fully susceptible to synthetic pyrethroid (deltamethrin) while being tolerant to organophosphorous (malathion). Susceptibility of An. culicifacies from 156 villages indicated that only 3 populations of An. culicifacies were resistant to deltamethrin while 57 populations were fully susceptible and other 96 populations were tolerant to deltamethrin. Resistance was recorded in 25 populations of An. culicifacies against malathion and 30 populations were tolerant to malathion insecticide. Remaining populations of An. fluviatilis and An. culicifacies were highly resistant to organochlorine. Results of cone bioassay revealed the mortality ranged from 32.5-51.1% on cemented and 27.5-43.3% on the mud wall sprayed with lambda cyhalothrin. Conclusion: The current study indicates that resistance has developed to synthetic pyrethroids in the major malaria vector An. culicifacies. Therefore, there is an urgent need for the evaluation of new insecticide molecules for better control of malaria vectors.

Contemporary Resistance Status of Anopheles Stephensi against Insecticides in District Dungarpur, Rajasthan, India

Pyrethroids are powerful insecticides used in the vector control program with impregnated mesh, and residual indoor sprays. However, resistance to insecticide reduces the effectiveness. The present susceptibility study carried out against theAnopheles Stephensi to monitor the sensitivity conditions of An. Stephensi vector, which raises the need to understand the state of vector resistance in the Dungarpur region of Rajasthan, India in order to better report vector-based interventions. The sensitivity study was carried out by the WHO standard method using recommended diagnostic doses of DDT, alpha-cypermethrin, permethrin, and deltamethrin. An. Stephensi showed resistance to DDT from the entire study while sensitive to alpha-cypermethrin, permethrin, and deltamethrin. The study looks at the selection and circulation of the appropriate insecticide’s molecule for a vector control program as insecticide need constant monitoring to develop effective vector control strategies such as improving insecticide by applying integrated biological and ecological methods.

Assessment of the Effect of C-Peptide Level on Na-K ATPase Activity In Individuals with Type II Diabetic Peripheral Neuropathy

Background: Both Type 1 and Type 2 diabetes can cause neuropathy, which is a frequent and severe consequence. C-peptide depletion may be partly related to in the occurrence of certain diabetic complications. It has been demonstrated that even a little amount of residual C-peptide has a considerable metabolic advantage. Objective: The study’s objective was to predict the relation of plasma C-peptide levels in patients with diabetic neuropathy, and its effect on Na-K ATPase activity. Design and Methods: In this case-control study, 150 individuals have been included: 80 patients with diabetic neuropathy, 40 diabetics without neuropathy and 30 non-diabetic subjects as a control. Patients in the first group were carefully chosen based on their clinical symptoms and nerve conduction studies results. The assessment of plasma C-peptide was done by ELISA, Na-K ATPase enzyme activity by spectrophotometer, and HbA1C by HPLC. Results: Mean plasma C-peptide level and Erythrocyte Na-K ATPase activity were substantially lower in neuropathy type 2 DM patients compared to diabetes without neuropathy and control (p= 0.002, 0.000 respectively). The negative correlation between C-peptide with HbA1c, and diabetes period were all negligible (p= 0.447,0.098), Even though there was a notable negative correlation with age (p= 0.03). On the other hand, the relationship linking C-peptide and Na-K ATPase enzyme activity was shown to be insignificant (p=0.69). Conclusions: Diabetic neuropathy is related to a low C-peptide level. The association between C-peptide and Na-K ATPase enzyme activity, on the other hand, was shown to be insignificant. C-peptide HbA1c, and duration of diabetes all had minor negative associations.

Critically Ill Patients with H1N1 Pneumonia: Two Year Experience in a Tertiary Level Indian ICU

Purpose: To learn about the clinical profile, outcome and quality of life and factors influencing these, in critically ill patients with H1N1 pneumonia. Methods: Retrospective analysis of case files and phone interview of 88patients with confirmed H1N1 pneumonia. Results: Out of 88 patients, 51 were males. Mean age was 48.23 [± 13.03]. 39 [44.31%] were in the 31-50 years age group and 37 [42.04%] were in the 51-70 age group. Diabetes [n=16] and Hypertension [n=20] were the most common comorbidities. Majority of the patients presented with cough [n=87], breathlessness [n=85] and fever [n=84]. 43 patients had severe ARDS on admission. Mean APACHE II score was 9.6 [± 5.4] Mean SOFA scores 4.99 [± 2.6]. Mean Murray score was 2.37 [± 0.76]. 46 patients [52%] survived. Factors associated with mortality were APACHE score [p=0.00], SOFA score [p=0.00] Murray score, severe ARDS [p=0.00], requirement of vasopressor support [p=0.00] or renal replacement therapy [p=0.00] and incidence of VAP [p=0.039]. Diabetes had a protective effect [p=0.04], as had non-invasive ventilation [p=0.00]. Murray score [p=0.000, SOFA score [p=0.036], initiation of mechanical ventilation [p=0.003] and incidence of VAP [p=0.00] was associated with increased length of stay among the survivors. Conclusion: Higher lung specific severity scores, severe ARDS, secondary organ failure and VAP were associated with increased mortality. Among survivors, higher Murray and SOFA scores, mechanical ventilation and vasopressor use entailed a longer ICU stay.

Ascorbic Acid as an Inhibitor for SARS-CoV-2 Virus Reproduction: A Theoretical Approach

In the present study, ascorbic acid’s or Vitamin C’s influence (VC) in inhibition of SARS-CoV-2 virus reproduction was investigated. Gaussian 09 with a basis set of 6-311G (d, p), MGL tools, DSV, and LigPlus software were used. According to the Total Electron Density (TED) and Millikan charges, the active sites for adsorption were determined. Further, the docking study had clearly revealed the role of VC in inhibition of the virus reproduction in accordance with binding energy (Eb) and ligand efficiency (LE). The vitamin’s interaction with the virus’s spikes may limit its replication or provide the immune system sufficient time to recognize the infection, which enhances the possibility of producing appropriate antibodies.

Secondary Attack Rate among the Contacts of COVID-19 Patients at the Beginning of the Pandemic in Pune City of WesternMaharashtra, India

Introduction: The COVID-19 was the emerging disease caused by SARS-COV 2. Efficient transmission of this disease occurs through droplets and fomites. As COVID-19 has high transmission and hence susceptible household & non-household close contacts are at high risk of developing disease. Study Objectives: 1) To estimate secondary attack rate of COVID-19 among the contacts during the pandemic in Pune city; 2) To identify factors associated with transmission and development of COVID-19 disease. Material & Methods: This was a retrospective cohort study conducted in the month of June 2020. The sample includes 741 contacts of 119 Laboratory confirmed cases for COVID-19, resides in 15 ward offices areas of Pune Municipal Corporation after written informed consent. A confidential telephonic interview was taken by using a prestructured questionnaire which includes socio demographic data, duration of stay, family background, outcome, type of house, development of symptoms etc. Results: The overall Secondary Attack Rate estimated to be 32.5%, 33.7% among the high risk contacts while 13.3% among the low risk contacts. In this city, 6 wards (40%) out of 15 wards showed SAR of more than 40%. The characteristics of primary cases associated with spread of disease were presence of symptoms and duration between onset of disease and isolation of primary case. The factors significantly affecting SAR were age, comorbidity, no. of family members, type of family, type of house, overcrowding, no. of rooms, bedroom attached with toilet, type of contact & containment zone. Conclusion: Higher SAR was seen in the household contacts. It is necessary to adopt rigorous measures to cut the transmission chain in this area of close contact.

Seasonal Prevalence and Host Preference of Some Medically Important Aedes Species of Doon Valley, India

In the recent past, several outbreaks of mosquito-borne diseases in Dehradun city make it necessary for a comprehensive understanding of man-vector contacts, disease transmission and determination of appropriate resting sites for adult mosquitoes. The study was conducted within the periphery of Dehradun city in Doon valley, Uttarakhand state, India consisting of eight (08) locations namely Sahaspur, Premnagar, Raipur, Karanpur, Sahastradhara, Dudhli, Doiwala and Rani Pokhri with the objective to analyze and assess the seasonal prevalence and host choice of the mosquitoes with special reference to Aedes species. Among the eight locations of the current study, Raipur registered the highest mosquito abundance (17%) trailed by Sahaspur (16%) and Karanpur (15%). A low abundance of mosquitoes was registered conjointly from Doiwala and Rani Pokhri areas (9% from each area). In these areas, the profile plots of the 2-way ANOVA displayed the highest variation for the data collected from human habitations followed by bovine sheds, whereas the lowest recorded variation was from the poultry. With regards to seasonal prevalence, the highest mosquito abundance was registered during the monsoon followed by the post-monsoon. In provender analysis, a total of 1912 blood meals were collected for antibody test among which 1851 were found positive, with the highest reaction with human antisera (57.75%), followed by bovine antisera (21.34%) and pigs antisera (4.97%). Ae. aegyptihad the highest Anthropophilic Index (A.I.) with 68.78%, followed by Ae. albopictus (64.89%) and Ae. vittatus (55.55%). However, Human Blood Predominance (H.B.P.) and Pure Human Blood Pervasiveness (P.H.B.P.) were found highest for Ae. albopictus with values 0.43 and 0.44 respectively. The results also show the seasonal prevalence and feeding habits of these vectors. This study would be helpful in formulating season-wise strategies for the control of Aedes vector mosquitoes in different eco-epidemiological situations.