The Study of Biochemical Markers in Chronic Renal Failure Patients in Pre- and Post-Haemodialysis

Background: The current research is designed to investigate alterations in lipid peroxidation (malondialdehyde - MDA) and renal markers (urea and creatinine) in patients of chronic renal failure (CRF) as compared to the control group. Method: The study included 55 subjects, who were separated into two groups: control group, which included 15 healthy members with no history of systematic illness; and patients group, which included 40 patients with CRF divided into four groups “Non: CRF patients without any accompanied disease, DM: CRF patients with diabetes mellitus, HT: CRF patients with hypertension, and HT + DM: CRF patients with diabetes mellitus and hypertension”. Results: The findings reveal that there is a notable increase in serum concentration of MDA, urea, and creatinine, in patients group as compared to the control group. Conclusion: In all chronic renal failure patients with or without any accompanying disease, lipid peroxidation is present in pre- and post-haemodialysis patients as well as patients with CRF have high levels of urea and creatinine compared with healthy groups.

An Assessment of Prominent Risks Factors Behind the Development of COVID-19 Pandemic Digital Eye Strain in context with the Online Class Room

The COVID-19 outbreak has destructively affected the education system over the world and brought about prominent alterations in the traditional systems of teaching and learning. In order to flatten the curve of disease transmission, several countries have resorted to online classes for students. Apart from the benefits of this strategy, there exists a chance of developing ocular complications in students those are continuously viewed the digital screen without any break. T-test and frequency analysis was used to find out the link between the digital electronic gadgets and eye strains. SPSS version 24.0.0 was used for all the statistical analyses. Here we assessed the various prominent factors behind the development of complications in eyes instigated by the electronic gadgets with special inference on the type of electronic gadgets, time spend, distance, type of usage, kind of posture, light condition, stress, and kind of symptoms towards digital eye strains. One of the major fact that has been emerged in this investigation is that the usage of electronic gadgets for online classes purposes have greatly increased than the other usage. The use of electronic gadgets for work and educational purposes has been recognized as a normal activity among individuals. With the extensive prevalence of developing digital eye strain in the students, it is paramount that the results of this investigation can be used for research purposes as strong research evidence to prevent the complications allied with eyes.

Insecticides Susceptibility Status of Malaria Vectors in a High Malaria Endemic Tribal District Gadchiroli (Maharashtra) of India

Background and Objective: The current study was undertaken to determine insecticide susceptibility of malaria vectors in various villages of high malaria endemic PHCs of Gadchiroli district of Maharashtra. Methods: Adult malaria vectors were collected from the human dwellings/ cattle sheds of 156 villages of 18 malaria endemic PHCs. Susceptibility tests were carried out for different insecticides against An. culicifacies and An. fluviatilis mosquitoes as per the World Health Organization (WHO) procedure. Cone bioassays were also done to assess the quality and efficacy of indoor residual spray. Results:An. fluviatilis could be collected from 23 villages only and all the populations were fully susceptible to synthetic pyrethroid (deltamethrin) while being tolerant to organophosphorous (malathion). Susceptibility of An. culicifacies from 156 villages indicated that only 3 populations of An. culicifacies were resistant to deltamethrin while 57 populations were fully susceptible and other 96 populations were tolerant to deltamethrin. Resistance was recorded in 25 populations of An. culicifacies against malathion and 30 populations were tolerant to malathion insecticide. Remaining populations of An. fluviatilis and An. culicifacies were highly resistant to organochlorine. Results of cone bioassay revealed the mortality ranged from 32.5-51.1% on cemented and 27.5-43.3% on the mud wall sprayed with lambda cyhalothrin. Conclusion: The current study indicates that resistance has developed to synthetic pyrethroids in the major malaria vector An. culicifacies. Therefore, there is an urgent need for the evaluation of new insecticide molecules for better control of malaria vectors.

Critically Ill Patients with H1N1 Pneumonia: Two Year Experience in a Tertiary Level Indian ICU

Purpose: To learn about the clinical profile, outcome and quality of life and factors influencing these, in critically ill patients with H1N1 pneumonia. Methods: Retrospective analysis of case files and phone interview of 88patients with confirmed H1N1 pneumonia. Results: Out of 88 patients, 51 were males. Mean age was 48.23 [± 13.03]. 39 [44.31%] were in the 31-50 years age group and 37 [42.04%] were in the 51-70 age group. Diabetes [n=16] and Hypertension [n=20] were the most common comorbidities. Majority of the patients presented with cough [n=87], breathlessness [n=85] and fever [n=84]. 43 patients had severe ARDS on admission. Mean APACHE II score was 9.6 [± 5.4] Mean SOFA scores 4.99 [± 2.6]. Mean Murray score was 2.37 [± 0.76]. 46 patients [52%] survived. Factors associated with mortality were APACHE score [p=0.00], SOFA score [p=0.00] Murray score, severe ARDS [p=0.00], requirement of vasopressor support [p=0.00] or renal replacement therapy [p=0.00] and incidence of VAP [p=0.039]. Diabetes had a protective effect [p=0.04], as had non-invasive ventilation [p=0.00]. Murray score [p=0.000, SOFA score [p=0.036], initiation of mechanical ventilation [p=0.003] and incidence of VAP [p=0.00] was associated with increased length of stay among the survivors. Conclusion: Higher lung specific severity scores, severe ARDS, secondary organ failure and VAP were associated with increased mortality. Among survivors, higher Murray and SOFA scores, mechanical ventilation and vasopressor use entailed a longer ICU stay.

Ascorbic Acid as an Inhibitor for SARS-CoV-2 Virus Reproduction: A Theoretical Approach

In the present study, ascorbic acid’s or Vitamin C’s influence (VC) in inhibition of SARS-CoV-2 virus reproduction was investigated. Gaussian 09 with a basis set of 6-311G (d, p), MGL tools, DSV, and LigPlus software were used. According to the Total Electron Density (TED) and Millikan charges, the active sites for adsorption were determined. Further, the docking study had clearly revealed the role of VC in inhibition of the virus reproduction in accordance with binding energy (Eb) and ligand efficiency (LE). The vitamin’s interaction with the virus’s spikes may limit its replication or provide the immune system sufficient time to recognize the infection, which enhances the possibility of producing appropriate antibodies.

Secondary Attack Rate among the Contacts of COVID-19 Patients at the Beginning of the Pandemic in Pune City of WesternMaharashtra, India

Introduction: The COVID-19 was the emerging disease caused by SARS-COV 2. Efficient transmission of this disease occurs through droplets and fomites. As COVID-19 has high transmission and hence susceptible household & non-household close contacts are at high risk of developing disease. Study Objectives: 1) To estimate secondary attack rate of COVID-19 among the contacts during the pandemic in Pune city; 2) To identify factors associated with transmission and development of COVID-19 disease. Material & Methods: This was a retrospective cohort study conducted in the month of June 2020. The sample includes 741 contacts of 119 Laboratory confirmed cases for COVID-19, resides in 15 ward offices areas of Pune Municipal Corporation after written informed consent. A confidential telephonic interview was taken by using a prestructured questionnaire which includes socio demographic data, duration of stay, family background, outcome, type of house, development of symptoms etc. Results: The overall Secondary Attack Rate estimated to be 32.5%, 33.7% among the high risk contacts while 13.3% among the low risk contacts. In this city, 6 wards (40%) out of 15 wards showed SAR of more than 40%. The characteristics of primary cases associated with spread of disease were presence of symptoms and duration between onset of disease and isolation of primary case. The factors significantly affecting SAR were age, comorbidity, no. of family members, type of family, type of house, overcrowding, no. of rooms, bedroom attached with toilet, type of contact & containment zone. Conclusion: Higher SAR was seen in the household contacts. It is necessary to adopt rigorous measures to cut the transmission chain in this area of close contact.

Seasonal Prevalence and Host Preference of Some Medically Important Aedes Species of Doon Valley, India

In the recent past, several outbreaks of mosquito-borne diseases in Dehradun city make it necessary for a comprehensive understanding of man-vector contacts, disease transmission and determination of appropriate resting sites for adult mosquitoes. The study was conducted within the periphery of Dehradun city in Doon valley, Uttarakhand state, India consisting of eight (08) locations namely Sahaspur, Premnagar, Raipur, Karanpur, Sahastradhara, Dudhli, Doiwala and Rani Pokhri with the objective to analyze and assess the seasonal prevalence and host choice of the mosquitoes with special reference to Aedes species. Among the eight locations of the current study, Raipur registered the highest mosquito abundance (17%) trailed by Sahaspur (16%) and Karanpur (15%). A low abundance of mosquitoes was registered conjointly from Doiwala and Rani Pokhri areas (9% from each area). In these areas, the profile plots of the 2-way ANOVA displayed the highest variation for the data collected from human habitations followed by bovine sheds, whereas the lowest recorded variation was from the poultry. With regards to seasonal prevalence, the highest mosquito abundance was registered during the monsoon followed by the post-monsoon. In provender analysis, a total of 1912 blood meals were collected for antibody test among which 1851 were found positive, with the highest reaction with human antisera (57.75%), followed by bovine antisera (21.34%) and pigs antisera (4.97%). Ae. aegyptihad the highest Anthropophilic Index (A.I.) with 68.78%, followed by Ae. albopictus (64.89%) and Ae. vittatus (55.55%). However, Human Blood Predominance (H.B.P.) and Pure Human Blood Pervasiveness (P.H.B.P.) were found highest for Ae. albopictus with values 0.43 and 0.44 respectively. The results also show the seasonal prevalence and feeding habits of these vectors. This study would be helpful in formulating season-wise strategies for the control of Aedes vector mosquitoes in different eco-epidemiological situations.

Diarrhoea and Associated Clinical Features in Different Pathotypes of Diarrheagenic E. coliIsolated in Children: A Case-Control Study in a Tertiary Care Hospital

Background: Acute gastroenteritis remains to be a major health problem in children causing high morbidity and mortalityIn India, diarrhoea is the third most common cause of death in children younger than 5 years of age, with an estimation of 300,000 deaths each year. Diarrheagenic Escherichia coli (DEC) being one of the important bacterial agents, the present hospital-based study was done to enlighten on the occurrence of different pathotypes and clinical features associated with DEC related diarrhoea. Methods:The case-control study was carried out in SCB Medical College, Odisha from October 2014 to September 2016 on the childhood diarrhoea cases (≤ 14 years of age). Stool samples were collected and processed to isolate the causative bacterial agent by standard biochemical test, serotyping and multiplex PCR. Results: 350 cases and 100 controls were included in the study. The different pathotypes of DEC were isolated significantly more in cases than control group (P value = 0.0205) with an isolation rate of 7.4% in cases. 12 (46.2%) of DEC were in 0-1 year age group followed by 1-5 year of age group i.e. 11 (42.3%) and least in 5-14 years of age group i.e. 3 (11.5%). The most common pathotype was Enterotoxigenic E. coli (ETEC) followed by Enteropathogenic E.coli (EPEC). Fever is the most common symptom associated with DEC diarrhoea; the other two common symptoms were watery diarrhoea and vomiting. Exclusive breastfeeding was the protective factor. Seasonal variation of DEC was found more among the cases in the rainy season. Conclusion: Different pathotypes of DEC being associated commonly with childhood diarrhoea, the correct identification of various types of DEC along with the clinical knowledge is important to reduce the morbidity and mortality caused by it.

The Effect of Immune Max in the Liver, Kidney and Spleen Histological Structure in Male Albino Mice

Background: This study aimed to identify the effect of using the Immune Max dietary supplement on the histological composition of some organs in male albino mice. Methods: The study was conducted on the Swiss albino male mice which were divided into two groups: treated group with 0.162 mg/kg/day of Immune Max and second group as a control. Results: Histological examination was conducted for liver, kidney and spleen. The results showed some histological changes in these organs like loses of tissues, congestion, leukocytes infiltration and dead cells. Conclusion: The purpose of a conclusion that the use of the Immune Max nutritional supplement can cause damage to the tissues of a number of organs and this is likely to be due to the dose concentration and the duration of the treatment.