Comparison of two fecal egg recovery techniques and larval culture for cyathostomins in horses

2009 ◽  
Vol 234 (9) ◽  
pp. 1161-1161
Author(s):  
Thomas R. Bello ◽  
Tammy M. Allen
Keyword(s):  
Larval Culture ◽  
Recovery Techniques

scholarly journals The production of excretory-secretory molecules from Heligmosomoides polygyrus bakeri fourth stage larvae varies between mixed and single sex cultures

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Marta Maruszewska-Cheruiyot ◽  
Ludmiła Szewczak ◽  
Katarzyna Krawczak-Wójcik ◽  
Magdalena Głaczyńska ◽  
Katarzyna Donskow-Łysoniewska
Keyword(s):  
Sex Pheromones ◽  
Pheromone Communication ◽  
Larval Culture ◽  
Heligmosomoides Polygyrus ◽  
Single Sex ◽  
Opposite Sex ◽  
Potential Vaccine ◽  
Immunomodulatory Properties ◽  
Therapeutic Compounds ◽  
Immunomodulatory Potential

Abstract Background Excretory-secretory (ES) products are crucial in maintaining helminths in the host. Consequently, the proteins of ES are potential vaccine molecules and potential therapeutic agents for autoimmune diseases. Heligmosomoides polygyrus bakeri, a gastrointestinal parasite of mice, is a model of hookworm infection in humans. ES produced by both sexes of H. polygyrus bakeri L4 stage cultured separately shows different immunomodulatory properties than ES obtained when both sexes are cultured together. Accordingly, the objective of this study was to identify and compare the excretory-secretory molecules from single-sex and mixed cultures. Methods The composition of ES of male and female L4 stage nematodes in the presence (cultured together) or absence (cultured alone) of the opposite sex was examined. Proteins were identified using mass spectrometry. The functions of identified proteins were explored with Blast2GO. Results A total of 258 proteins derived from mixed larval culture in the presence of sex pheromones were identified, 160 proteins from pure female cultures and 172 from pure male cultures. Exposure of nematodes to the sex pheromones results in abundant production of proteins with immunomodulatory properties such as Val proteins, acetylcholinesterases, TGF-β mimic 9 and HpARI. Proteins found only in ES from mixed larval cultures were TGF-β mimics 6 and 7 as well as galectin. Conclusions The presence of the opposite sex strongly influences the composition of ES products, probably by chemical (pheromone) communication between individuals. However, examination of the composition of ES from various conditions gives an opportunity for searching for new potentially therapeutic compounds and anthelminthics as well as components of vaccines. Manipulation of the nematode environment might be important for the studies on the immunomodulatory potential of nematodes.

scholarly journals Brief materials and programmes for children and families who have experienced conflict and displacement

2020 ◽  
Vol 30 (Supplement_5) ◽  
Author(s):  
R Calam ◽  
A El-Khani
Keyword(s):  
Mental Health ◽  
Traumatic Stress ◽  
Children And Families ◽  
Post Traumatic Stress ◽  
Parenting Skills ◽  
Conflict Zones ◽  
Conflict Zone ◽  
Care Workers ◽  
Recovery Techniques ◽  
Post Traumatic

Abstract This presentation will provide an overview of a set of materials and programmes designed for families who are living through or have escaped conflict and displacement. Delivery formats include i) brief leaflets giving parenting advice for caregivers; ii) a more extensive booklet and format for a Conversation Group, and iii) a programme designed to address post-traumatic stress in children, Teaching Recovery Techniques (TRT), to which a caregiver and parenting skills module was added, to form TRT+Parenting. Each intervention has been tested or trialled with families living in challenged contexts. The leaflet was distributed to 3000 families via bakeries in conflict zones in Syria. The Conversation Group was trialled for feasibility in Palestine. TRT+Parenting was piloted with Syrian families living on the Turkey-Syria border, and then trialled with 120 families in Lebanon, with teachers and social care workers trained as facilitators. In Syria, 59.5% of 3000 parents returned questionnaires and reported satisfaction with the leaflet despite the study being conducted in a conflict zone and in the context of humanitarian intervention. The Conversation Group proved feasible and acceptable, and caregivers in Palestine gave positive feedback. Children and caregivers showed significant improvements across measures of trauma and mental health, with higher levels of improvement seen in the TRT+Parenting group compared to TRT alone or waitlist. Caregivers who took part in the Plus Parenting component also reported improvement in their own mental health. The results demonstrated valuable improvements across all interventions, and indicate that brief programs can and should be widely used as components of preventive strategies.

scholarly journals Sheep nemabiome diversity and its response to anthelmintic treatment in Swedish sheep herds

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Peter Halvarsson ◽  
Johan Höglund
Keyword(s):  
Species Richness ◽  
Diversity Index ◽  
Nematode Species ◽  
Abundant Species ◽  
Anthelmintic Treatment ◽  
Host Age ◽  
Larval Culture ◽  
Nematode Parasites ◽  
South Central ◽  
Nematode Diversity

Abstract Background A novel way to study the species composition and diversity of nematode parasites in livestock is to perform deep sequencing on composite samples containing a mixture of different species. Herein we describe for the first time the nematode community structures (nemabiomes) inhabiting Swedish sheep and how these are/were affected by host age and recent anthelmintic treatments. Methods A total of 158 fecal samples were collected (n = 35 in 2007 and n = 123 in 2013–2016) and cultured from groups of sheep on 61 commercial farms in the south-central part of the country where most animals are grazed. Among the samples, 2 × 44 (56%) were paired collections from the same groups pre- and post-treatment with anthelmintics such as macrocyclic lactones, benzimidazoles or levamisole. Samples were analyzed for their nemabiome using the PacBio platform followed by bioinformatic sequence analysis with SCATA. Species richness and diversity were calculated and analyzed in R. Results Nematode ITS2 sequences were found in all larval culture samples except two, even though the fecal egg counts were below the McMaster threshold in 20 samples. Sequencing yielded, on average, 1008 sequences per sample. In total, 16 operational taxonomical units (OTU), all with ≥ 98 % identity to sequences in the NCBI database, were recognized. The OTUs found represented nematode species of which ten are commonly associated with sheep. Multiple species were identified in all pre-anthelmintic treatment larval culture samples. No effects on nematode diversity were found in relation to host age. On the other hand, recent anthelmintic treatment lowered species richness, especially after use of ivermectin and albendazole. Interestingly, despite zero egg counts after use of levamisole, these samples still contained nematode DNA and especially H. contortus. Conclusions Our findings provide evidence that nemabiome analysis combined with diversity index analysis provides an objective methodology in the study of the efficacy of anthelmintic treatment as both high and low abundant species were detected.

scholarly journals Error Detection and Recovery Techniques for Variation-Aware CMOS Computing: A Comprehensive Review

2011 ◽  
Vol 1 (3) ◽  
pp. 334-356 ◽  
Author(s):  
Joseph Crop ◽  
Evgeni Krimer ◽  
Nariman Moezzi-Madani ◽  
Robert Pawlowski ◽  
Thomas Ruggeri ◽  
...  
Keyword(s):  
Error Detection ◽  
Comprehensive Review ◽  
Recovery Techniques

Applicability of Enhanced Oil Recovery techniques on mature fields - Interest of gas injection

2005 ◽  
Author(s):  
Frederic Maubeuge ◽  
Danielle Christine Morel ◽  
Jean-Pierre Charles Fossey ◽  
Said Hunedi ◽  
Jacques Albert Danquigny
Keyword(s):  
Enhanced Oil Recovery ◽  
Oil Recovery ◽  
Gas Injection ◽  
Recovery Techniques

Computer Implementation of the Dykstra-Parsons Method of Waterflood Calculation

2021 ◽  
Author(s):  
Prosper Kiisi Lekia
Keyword(s):  
Petroleum Industry ◽  
Injection Pressure ◽  
Injection Rate ◽  
Process Time ◽  
Computer Implementation ◽  
Natural Energy ◽  
Recovery Techniques ◽  
Secondary Recovery ◽  
Time Required ◽  
User Friendly

Abstract One of the challenges of the petroleum industry is achieving maximum recovery from oil reservoirs. The natural energy of the reservoir, primary recoveries in most cases do not exceed 20%. To improve recovery, secondary recovery techniques are employed. With secondary recovery techniques such as waterflooding, an incremental recovery ranging from 15 to 25% can be achieved. Several theories and methods have been developed for predicting waterflood performance. The Dykstra-Parson technique stands as the most widely used of these methods. The authors developed a discrete, analytical solution from which the vertical coverage, water-oil ratio, cumulative oil produced, cumulative water produced and injected, and the time required for injection was determined. Reznik et al extended the work of Dykstra and Parson to include exact, analytical, continuous solutions, with explicit solutions for time, constant injection pressure, and constant overall injection rate conditions, property time, real or process time, with the assumption of piston-like displacement. This work presents a computer implementation to compare the results of the Dykstra and Parson method, and the Reznik et al extension. A user-friendly graphical user interface executable application has been developed for both methods using Python 3. The application provides an interactive GUI output for graphs and tables with the python matplotlib module, and Pandastable. The GUI was built with Tkinter and converted to an executable desktop application using Pyinstaller and the Nullsoft Scriptable Install System, to serve as a hands-on tool for petroleum engineers and the industry. The results of the program for both methods gave a close match with that obtained from the simulation performed with Flow (Open Porous Media). The results provided more insight into the underlying principles and applications of the methods.

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