Using stomach contents for diet analysis of carnivores through DNA barcoding

2015 ◽  
Vol 11 (1) ◽  
Author(s):  
Teresa Santos ◽  
Carlos Fonseca ◽  
Tânia Barros ◽  
Raquel Godinho ◽  
Cristiane Bastos-Silveira ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Stomach Contents ◽  
Diet Analysis

DNA barcoding increases resolution and changes structure in Canadian boreal shield lake food webs

DNA Barcodes ◽  
2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Timothy J. Bartley ◽  
Heather E. Braid ◽  
Kevin S. McCann ◽  
Nigel P. Lester ◽  
Brian J. Shuter ◽  
...  
Keyword(s):  
Food Web ◽  
Food Webs ◽  
Dna Barcoding ◽  
Stomach Contents ◽  
Diet Analysis ◽  
Food Web Structure ◽  
Web Structure ◽  
Habitat Coupling ◽  
Boreal Shield ◽  
Predatory Fishes

AbstractFood webs are important in understanding the structure, function, and behaviour of ecosystems, but, due to methodological limitations, are often poorly resolved in ways that impact food-web properties. Although DNA barcoding has proven useful in determining the diet of consumers, few studies have used this technique to determine food-web structure. These studies report mixed impacts on various food-web properties, but are limited by their taxonomic focus and their failure to evaluate DNA barcoding for both diet analysis and food-web structure. In this study, we show that, when compared to a morphological approach, DNA barcoding increases foodweb resolution by increasing the number and frequency of prey species identified in the stomach contents of eight species of Canadian boreal shield predatory fishes. In addition, we observed differences in food-web structure, such as increased generalism, habitat coupling, and omnivory, that have strong implications for food-web stability and dynamics. We conclude that DNA barcoding is a powerful tool to evaluate how resolution impacts foodweb properties and can help further our understanding of how food webs are structured by identifying feeding interactions in an unprecedented and highly detailed manner.

scholarly journals Variability in the diet diversity of catfish highlighted through DNA barcoding

2020 ◽  
Author(s):  
Chinnamani Prasannakumar ◽  
Gunasekaran Iyyapparajanarasimapallavan ◽  
M. Ashiq Ur Rahman ◽  
P. Mohanchander ◽  
T. Sudhakar ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Monsoon Season ◽  
Stomach Contents ◽  
Hermit Crabs ◽  
Gut Contents ◽  
Reference Database ◽  
Perna Viridis ◽  
Fish Diet ◽  
Diet Diversity ◽  
Post Monsoon

AbstractIdentification and quantification of fish diet diversity was the first step in understanding the food web dynamics and ecosystem energetics, where the contribution of DNA barcoding technique has been important. We used DNA barcoding to identify the stomach contents of a euryhaline, benthophagous catfish Ariius maculatus. From 40 catfish stomach items sampled in two different seasons, we barcoded 67 piscine and macro-invertebrates prey items, identified as belonging to 13 species in 4 major phyla (viz., Chordate, Arthropod, Annelida and Mollusca). It is important to note that the mollusc taxa (Meritrix meritrix and Perna viridis) and a species of fish (Stolephorus indicus) could not be found among the gut contents of A. maculatus sampled during the pre- and post-monsoon season, respectively. Among the piscine diets of A. maculatus, Eubleekeria splendens (23.5%) and Stolephorus indicus (23.5%) were the major prey taxa during pre-monsoon season. The hermit crabs forms the major constituents of both pre- and post-monsoon seasons, among invertebrate taxa. Polychaete, Capitella capitata (25.92%) was abundantly consumed invertebrates next to hermit crabs. We noticed that in pre-monsoon A. maculatus was more piscivorous than post-monsoon. As revealed through Kimura-2 parametric pair­wise distance analysis, the diet diversity was relatively higher in post-monsoon. The accumulation curve estimated 57 haplotypes within 14 barcoded species (including the host A. maculatus). Majority of haplotypes were found among fishes (47.36%) followed by Arthropods (28.07%), Annelids (14.03%) and Mollusca (10.52%), respectively. This study also highlights that there is a growing concern about A. maculatus’s aggressive predation on commercially important stocks of fish and invertebrates. We will continue to expand the coverage of species barcoded in the reference database, which will become more significant as meta- and environmental DNA barcoding techniques become cheaper and prevalent.

scholarly journals New tools for diet analysis: nanopore sequencing of metagenomic DNA from rat stomach contents to quantify diet

2020 ◽  
Author(s):  
Nikki Freed ◽  
William Pearman ◽  
Adam Smith ◽  
Georgia Breckell ◽  
James Dale ◽  
...  
Keyword(s):  
Stomach Contents ◽  
Diet Analysis ◽  
Nanopore Sequencing ◽  
Rat Stomach ◽  
Metagenomic Dna

A Comparison of Stomach and Fecal Samples for Diet Analysis of Grey Kangaroos

1988 ◽  
Vol 15 (3) ◽  
pp. 249 ◽  
Author(s):  
GL Norbury
Keyword(s):  
Plant Species ◽  
Stomach Contents ◽  
Microscopic Analysis ◽  
Dry Weight ◽  
Stomach Content Analysis ◽  
Diet Analysis ◽  
Faecal Analysis ◽  
Differential Digestion ◽  
Free Ranging ◽  
Species Proportions

In an experiment with four captive eastern grey kangaroos, Macropus giganteus, microscopic analysis of both stomach contents and faeces did not reflect the ingested dry weight proportion of most plant species in a fed diet. However, close agreement was obtained by correcting for unidentifiable microscopic fragments of plants. This suggests that differential digestion may not be as important an artefact of microscopic diet analysis as previously reported. Provided corrections are made, faecal analysis may be as reliable as stomach content analysis for describing relative proportions of plant species in the diet of macropodids. In contrast, differences in proportions of plant species between stomach and faecal samples from free-ranging western grey kangaroos, Macropus fuliginosus, suggest that faecal analysis may not be suitable for quantitative studies of diet selection (comparisons of plant species proportions in the diet with species proportions in the field) in areas with heterogeneous vegetation. In these situations, samples of stomach contents are considered more appropriate.

scholarly journals Morphological identification and DNA barcoding used for diet analysis of gilthead seabream (Sparus aurata) in its expanding northerly range

2017 ◽  
Vol 30 ◽  
pp. 1 ◽  
Author(s):  
S. Avignon ◽  
E. Tastard ◽  
S. Weston ◽  
G. Duhamel ◽  
F. Denis
Keyword(s):  
Dna Barcoding ◽  
Sparus Aurata ◽  
Atlantic Coast ◽  
Relative Proportion ◽  
Diet Analysis ◽  
Gilthead Seabream ◽  
Morphological Identification ◽  
Fish Length ◽  
Preliminary Evaluation ◽  
English Channel

The gilthead seabream, Sparus aurata, is common in the Mediterranean Sea and along the Atlantic coasts of Portugal, Spain and France. Abundance of S. aurata has recently increased along the Brittany coast, showing good adaptation and acclimatisation to northern waters away from its original distribution range. The physiological adaptations (diet, reproduction, growth) of this fish to colder water could even lead to its colonisation of the English Channel. The ability to eat and digest hard prey makes this fish an important consumer of bivalves. The aim of this study was to make a preliminary evaluation of the diet of the gilthead seabream in its northern range of distribution. Prey items from stomach contents of wild adults from various sites along the East Atlantic coast of France to the English Channel were identified morphologically when it was possible, e.g. in presence of decapod appendices, shells of bivalves, or using DNA barcoding. Diet composition was analysed against sites, fish length and month of sampling using the frequency of occurrence (%F) and weight relative proportion (%W). Results showed that the diet of S. aurata was mainly composed of bivalves, malacostracans and gastropods with a huge dominance of Mytilus sp. (%F = 51.5 and %W = 40.2). This first diet analysis of individuals from the northern range of the species distribution showed its ability, as an opportunistic feeder, to find prey in newly colonised ecosystems and its preference for some organisms, especially mussels.

New perspectives in diet analysis based on DNA barcoding and parallel pyrosequencing: thetrnL approach

2009 ◽  
Vol 9 (1) ◽  
pp. 51-60 ◽  
Author(s):  
ALICE VALENTINI ◽  
CHRISTIAN MIQUEL ◽  
MUHAMMAD ALI NAWAZ ◽  
EVA BELLEMAIN ◽  
ERIC COISSAC ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Diet Analysis ◽  
Parallel Pyrosequencing

Effectiveness of DNA barcoding for identifying piscine prey items in stomach contents of piscivorous catfishes

2015 ◽  
Vol 99 (1) ◽  
pp. 161-167 ◽  
Author(s):  
Z. Moran ◽  
D. J. Orth ◽  
J. D. Schmitt ◽  
E.M. Hallerman ◽  
R. Aguilar
Keyword(s):  
Dna Barcoding ◽  
Stomach Contents ◽  
Prey Items

scholarly journals Changes in the diet composition of Pygmy Cormorant Phalacrocorax pygmeus on Skadar lake (Southern Montenegro)

Acrocephalus ◽  
2010 ◽  
Vol 31 (144) ◽  
pp. 21-26
Author(s):  
Andrej Vizi ◽  
Ondrej Vizi
Keyword(s):  
Breeding Season ◽  
Fish Species ◽  
Fish Community ◽  
Diet Composition ◽  
Stomach Contents ◽  
Breeding Population ◽  
Diet Analysis ◽  
Regional Population ◽  
Species Specific ◽  
Lake Fish

Changes in the diet composition of Pygmy Cormorant Phalacrocorax pygmeus on Skadar lake (Southern Montenegro) Diet changes of Pygmy Cormorant Phalacrocorax pygmeus, a piscivorous bird feeding primarily on fish up to 15 cm long, were studied on Skadar Lake. The breeding population on the lake was estimated at over 2,000 pairs in 2005 and 1,260 pairs in 2006, comprising about 5-8% of the regional population of SE Europe & Turkey. The first diet analysis, based on stomach contents of specimens hunted in the course of the breeding season, was carried out in the period 1973-1975 in order to identify the influence of the birds on commercial fishery. Further samples of regurgitated or accidentally dropped food items during the feeding of nestlings were collected and analyzed in the 2006 breeding season. Results from both periods are presented and changes in diet composition in the course of over 30 years are discussed. Between 1973-1975 and 2006, the Pygmy Cormorant's diet on Skadar Lake changed drastically. Only one fish species, Rudd Scardinius knezevici, was found to be common to both study periods. Pygmy Cormorant's diet in the period 1973-1975 consisted of 11 fish species (N = 224 specimens), belonging to five families. The most abundant fish species were Roach Rutilus ohridanus and Albanian Roach Pachychilon pictum. Diet analysis in 2006 revealed only six species (N = 98 specimens) from two families, and also showed that the introduced Goldfish Carassius auratus became a major food source for Pygmy Cormorant, constituting 84.7% of the total specimen number. Age class analysis of Goldfish items revealed that juvenile specimens (45-90 mm) comprised 89.0% of the total Goldfish compound. The study confirms that the Pygmy Cormorant is not a species-specific hunter and suggests it is not affected by the changes in composition of fish community in the littoral zone of the lake. Fish consumption by Pygmy Cormorants on Skadar Lake in 2006 was estimated at 45 t during the most intensive foraging period (June-August).

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