scholarly journals The Effects of Hyaluronic Acid, Calcium Hydroxide, and Dentin Adhesive on Rat Odontoblasts and Fibroblasts

2011 ◽  
Vol 62 (2) ◽  
pp. 155-161 ◽  
Author(s):  
Ana Bogović ◽  
Jana Nižetić ◽  
Nada Galić ◽  
Davor Želježić ◽  
Vedran Micek ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Calcium Hydroxide ◽  
Culture Medium ◽  
Calf Serum ◽  
Foetal Calf Serum ◽  
Sprague Dawley ◽  
Pulp Tissue ◽  
Pulp Capping ◽  
Sprague Dawley Rats ◽  
Dentin Adhesive

The Effects of Hyaluronic Acid, Calcium Hydroxide, and Dentin Adhesive on Rat Odontoblasts and FibroblastsThe aim of this study was to investigate the effects and efficiency of pulp capping preparations based on hyaluronic acid, calcium hydroxide, and dentin adhesive on the pulp tissue of Sprague-Dawley rats. The rats were killed and extracted teeth sectioned transversely through the pulp. The slices were placed in a RPMI 1640 cell culture medium supplemented with 10 % foetal calf serum. During 14 days of cultivation cultures were treated with preparations that contained hyaluronic acid (Gengigel Prof®), and calcium hydroxide (ApexCal®), or with dentin adhesive (Excite®). Cellularity and viability of fibroblasts and odontoblasts was analysed using a haemocytometer. Hyaluronic acid proved most efficient and the least toxic for direct pulp capping. Even though calcium hydroxide and dentin adhesive demonstrated a higher degree of cytotoxicity, their effects were still acceptable in terms of biocompatibility.

scholarly journals Nestin expressions of exposed pulp after direct pulp capping by calcium hydroxide and platelet rich plasma

2016 ◽  
Vol 10 (03) ◽  
pp. 341-344 ◽  
Author(s):  
Sartika Puspita ◽  
Totok Utoro ◽  
Tetiana Haniastuti
Keyword(s):  
Calcium Hydroxide ◽  
Platelet Rich Plasma ◽  
Sprague Dawley ◽  
Direct Pulp Capping ◽  
Nestin Expression ◽  
Pulp Tissue ◽  
Pulp Capping ◽  
Sprague Dawley Rats ◽  
Group 2 ◽  
Immunohistochemical Techniques

ABSTRACT Objective: The aim of this study was to evaluate nestin expression of pulp tissue following direct pulp capping with platelet-rich plasma (PRP). Materials and Methods: The thirty sound teeth from Sprague-Dawley rats were used and divided into two groups: Groups 1, teeth were capped with calcium hydroxide/Ca(OH)2 (n = 15) and Group 2 with PRP (n = 15). After 1st, 7th, and 21st days, respectively, 5 teeth each group (American Dental Association 41) were processed for light microscopic examination. Expressions of nestin were assessed by immunohistochemical techniques. Results: Nestin expression of Ca(OH)2 on the distance place of exposure at 1st and 7th days were 80% and at 21st day were 60%. Nestin expression of PRP on the distance place at 1st day was 80%, 7th 100%, and 21st day was 80%. At day 21 observation, Kruskal–Wallis test shows nestin expression was increased significantly in PRP groups (P < 0.05), but it was not increase significantly compare with Ca(OH)2. Conclusion: PRP had ability as a direct pulp capping material to induce nestin expression.

scholarly journals Clinical and Histological Evaluation of Direct Pulp Capping on Human Pulp Tissue Using a Dentin Adhesive System

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Alicja Nowicka ◽  
Ryta Łagocka ◽  
Mariusz Lipski ◽  
Mirosław Parafiniuk ◽  
Katarzyna Grocholewicz ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Third Molar ◽  
Adhesive System ◽  
Histological Evaluation ◽  
Control Group ◽  
Single Bond ◽  
Pulp Tissue ◽  
Pulp Capping ◽  
Hydroxide Group ◽  
Dentin Adhesive

Objective. This study presents a clinical and histological evaluation of human pulp tissue responses after direct capping using a new dentin adhesive system. Methods. Twenty-eight caries-free third molar teeth scheduled for extraction were evaluated. The pulps of 22 teeth were mechanically exposed and randomly assigned to 1 of 2 groups: Single Bond Universal or calcium hydroxide. Another group of 6 teeth acted as the intact control group. The periapical response was assayed, and a clinical examination was performed. The teeth were extracted after 6 weeks, and a histological analysis was performed. The pulp status was assessed, and the thickness of the dentin bridge was measured and categorized using a histological scoring system. Results. The clinical phase was asymptomatic for Single Bond Universal patients. Patients in the calcium hydroxide group reported mild symptoms of pain, although the histological examination revealed that dentin bridges with or without limited pulpitis had begun forming in each tooth. The universal adhesive system exhibited nonsignificantly increased histological signs of pulpitis (P>0.05) and a significantly weaker thin mineralized tissue layer (P<0.001) compared with the calcium hydroxide group. Conclusion. The results suggest that Single Bond Universal is inappropriate for human pulp capping; however, further long-term studies are needed to determine the biocompatibility of this agent.

scholarly journals THE EFFECT OF CALCIUM HYDROXIDE-PROPOLIS ON THE NUMBER OF MACROFAG CELLS ON THE WISTAR RAT DENTAL WITH PULPA PERFORMANCE

2020 ◽  
Vol 10 (2) ◽  
pp. 58
Author(s):  
Setyabudi Setyabudi ◽  
Devi Eka Juniarti ◽  
Ira Widjiastuti ◽  
Maughfirah Shintya Fathori
Keyword(s):  
Calcium Hydroxide ◽  
Cellular Response ◽  
Wistar Rats ◽  
Inflammatory Cells ◽  
Wistar Rat ◽  
Control Group ◽  
Pulp Tissue ◽  
Pulp Capping ◽  
Macrophage Cells ◽  
Combined Application

Background: Cellular response of dental pulp to existing lesions, caused by infiltration of inflammatory cells that migrate from blood vessels to the site of injury. Macrophages are the cells that most appear when inflammation occurs. Treatment for maintaining pulp tissue vitality is called pulp capping. Calcium hydroxide as a pulp capping material has the ability to trigger the growth of dentin bridges or remineralization, but calcium hydroxide can induce migration and proliferation of inflammatory cells. Currently the field of dentistry is developing propolis as an alternative pulp capping material. That is because propolis has anti-inflammatory properties. Objective: To analyze the effect of the combination of calcium hydroxide-propolis on the number of macrophage cells in the teeth of wistar rats with pulp perforation. Method: This study used 30 samples of Wistar rats which were preparated until perforation and then divided into 3 groups. The first group continued with filling with cention, the second group continued with application of calcium hydroxide and filled with cention, and the third group continued with the application of combination of calcium hydroxide and propolis and then being filledwithcention. Macrophage cell is calculated histopathologically by using compound light microscope on the 3rd day with 400x magnification. Results: The Kruskal-Wallis test results showed significant differences in macrophages after the combination of calcium hydroxide and propolis (p = 0,000). The combined application of calcium hydroxide and propolis showed higher macrophages than the application of calcium hydroxide and the control group. Conclusion: Application of calcium hydroxide - propolis combination was proven increase the number of macrophage cells in Wistar Rats (Rattus Norvegicus) with pulp perforation

scholarly journals Induction of reparative dentin by calcium silicate-based material as direct pulp capping agent

2019 ◽  
Vol 12 (4) ◽  
pp. 182-186
Author(s):  
Mozammal Hossain ◽  
Mahmood Sajedeen ◽  
Yukio Nakamura
Keyword(s):  
Calcium Hydroxide ◽  
Calcium Silicate ◽  
Normal Function ◽  
Direct Pulp Capping ◽  
Pulp Tissue ◽  
Pulp Capping ◽  
Exposure Site ◽  
Reparative Dentin ◽  
Dentin Formation ◽  
Reparative Dentin Formation

This study was performed to examine whether calcium silicate could induce reparative dentin formation without eliciting any adverse effect in direct pulp capping of premolar teeth. Twenty participants who need extraction of their 4 healthy permanent premolar teeth for orthodontic reasons were included in this study. Following the surgical procedure, the exposed pulp tissue was treated either with calcium silicate or covered with calcium hydroxide paste. On day 3, 7, 14 and 28, the experimental teeth was extracted and examined using light microscopy and histometric analysis to observe the inflammatory changes and the amount of reparative dentin formation. The results showed that in the calcium silicate treated teeth, substantial amounts of dentine-like tissue was formed on day 14 and mostly located on the exposure site. It was also observed in the calcium hydroxide treated teeth but dentin-like tissue located at a distance from the exposure site. The total amount of reparative dentine formed in the calcium silicate-treated teeth was significantly higher (p<0.005) than in the calcium hydroxide-treated specimens. In conclusion that the calcium silicate indices pulpal wound healing and reparative formation in the exposed teeth without affecting the normal function of the remaining pulp.

scholarly journals Immunoreactive insulin from mouse brain cells in culture and whole rat brain

1984 ◽  
Vol 218 (1) ◽  
pp. 19-27 ◽  
Author(s):  
N P Birch ◽  
D L Christie ◽  
A G C Renwick
Keyword(s):  
Rat Brain ◽  
Mouse Brain ◽  
Culture Medium ◽  
Calf Serum ◽  
Brain Cells ◽  
Immunoreactive Insulin ◽  
Foetal Calf Serum ◽  
Cells In Culture ◽  
Pancreatic Insulin ◽  
Contrast Microscopy

Foetal mouse brain cells were cultured as described previously [Sotelo, Gibbs, Gajdusek, Toh & Wurth (1980) Proc. Natl. Acad. Sci. U.S.A. 77, 653-657] without added insulin and without foetal calf serum after 12 days in culture. Examination by phase-contrast microscopy showed that these modifications did not appear to affect growth and development of the cells adversely. Silver impregnation of the cultures and indirect immunofluorescence following reaction with tetanus toxin showed that a high proportion of the cells resembled neurones. Analysis of concentrated culture medium by radioimmunoassay and high-pressure liquid chromatography (h.p.l.c.) revealed that the cells produced two main forms of immunoreactive insulin which differed from authentic pancreatic insulin in retention time. Immunoreactive somatostatin was also produced in culture and this was resolved into at least three forms by h.p.l.c. Immunoreactive insulin was also extracted from whole rat brain by using two published procedures. The method of Havrankova, Schmechel, Roth & Brownstein [Proc. Natl. Acad. Sci. U.S.A. (1978) 75, 5737-5741] consistently gave greater yields of insulin than did that of Eng & Yalow [Diabetes (1980) 29, 105-109] and the concentration was about three times that of plasma. The extracted insulin was further characterized by h.p.l.c. in each case and was found to behave like authentic pancreatic insulin. The production of insulin and somatostatin by foetal mouse brain cells in culture suggests that they may be a useful model system for studies of neuropeptide biosynthesis.

Immunocytochemical localization of a brush border hydrolase, lactase, in newborn suckling rat jejunum

1993 ◽  
Vol 51 ◽  
pp. 42-43
Author(s):  
László G. Kömüves ◽  
Mary A. Dudley ◽  
Buford L. Nichols
Keyword(s):  
Brush Border ◽  
Secretory Pathway ◽  
Calf Serum ◽  
Ultrastructural Localization ◽  
Sprague Dawley ◽  
Rat Pups ◽  
Sprague Dawley Rats ◽  
Intracellular Compartments ◽  
Small Intestinal ◽  
Coated Carbon

Lactase-phlorizin hydrolase (LPH, EC 3.2.1.23), an integral membrane glycoprotein of the small intestinal brush border, converts lactose, the main carbohydrate in milk, to its monosaccharide components. Although the activity of LPH is high in suckling rats, little is known about its distribution within the intracellular compartments of the secretory pathway and brush border. We present the first description of the ultrastructural localization of LPH in the neonatal jejunum of suckling rat pups.Pieces of jejunum from 12- to 14-d-old suckling rat pups from three litters of Sprague- Dawley rats were fixed with 4% freshly prepared paraformaldehyde in 100 mM phosphate buffer, pH=7.40, for 4 h, and stored in 1% paraformaldehyde, at 4°C, until further processing. The samples were sectioned after cryoprotection in 2.3 M sucrose in an RMC MT-7 ultramicrotome equipped with CR21 cryoattachment. Ultrathin cryosections were collected on Formvar-coated, carbon-evaporated nickel grids. The nonspecific binding sites were blocked by 1% heat-inactivated newborn calf serum in 10 mM Trizma buffer, pH=7.60, containing 500 mM NaCl, 0.05% NaN3 and 20 mM glycine (buffer A).

CORRELATION BETWEEN THE EFFECTS OF HORMONES ON THE SYNTHESIS OF DNA IN EXPLANTS FROM INDUCED RAT MAMMARY TUMOURS AND THE GROWTH OF THE TUMOURS

1974 ◽  
Vol 62 (2) ◽  
pp. 225-240 ◽  
Author(s):  
D. LEWIS ◽  
R. C. HALLOWES
Keyword(s):  
Organ Culture ◽  
Dna Synthesis ◽  
Culture Medium ◽  
Mammary Glands ◽  
Sprague Dawley ◽  
Culture Techniques ◽  
Mammary Tumours ◽  
Sprague Dawley Rats

SUMMARY Explants from 32 mammary tumours induced in Sprague—Dawley rats by 9,10-dimethyl-1,2-benzanthracene (DMBA) were maintained in organ culture for up to 48 h. Insulin, corticosterone, prolactin, growth hormone and oestradiol were added to the culture medium in various combinations and their effects on the DNA synthesis of the explants was studied. DNA synthesis was stimulated by insulin in explants from 30 out of the 32 tumours examined and this group of 30 responsive tumours could be further subdivided. Explants from 16 tumours showed a greater rate of DNA synthesis in medium containing insulin plus corticosterone plus prolactin than in medium containing insulin alone and this higher rate was decreased by oestradiol; this group is referred to as 'prolactin-responsive'. Explants from the remaining 14 tumours did not show a greater rate of DNA synthesis in medium that contained insulin plus corticosterone plus prolactin than in medium containing insulin alone and neither rate was decreased by oestradiol; this group is referred to as 'insulin-responsive'. Explants from two tumours were not stimulated by insulin and these tumours are referred to as 'non-responsive'. After oophorectomy or administration of ergocryptine to tumour-bearing rats, the prolactin-responsive tumours regressed whereas the non-responsive tumours continued to grow. Explants taken from prolactin-responsive tumours 2 weeks after either oophorectomy or administration of ergocryptine were still prolactin-responsive but those taken from insulin-responsive tumours 2 weeks after the same treatment were now also prolactin-responsive. The non-responsive tumours remained non-responsive. The effects of hormones on the DNA synthesis in vitro of explants from growing DMBA-induced tumours were thus different from those on explants of mammary glands from virgin or pregnant Sprague—Dawley rats. It was concluded that it was possible to predict by organ culture techniques the response in vivo of growing mammary tumours to oophorectomy and ergocryptine administration.

Repair of subtotal tympanic membrane perforations with Seprafilm®

2004 ◽  
Vol 118 (11) ◽  
pp. 862-865 ◽  
Author(s):  
Ersin Konakçi ◽  
Mehmet Koyuncu ◽  
Recep Ünal ◽  
Atilla Tekat ◽  
Melek Uyar
Keyword(s):  
Hyaluronic Acid ◽  
Tympanic Membrane ◽  
Control Group ◽  
Sprague Dawley ◽  
Tympanic Membrane Perforations ◽  
Sprague Dawley Rats ◽  
First Choice ◽  
The Mean ◽  
Experimental Use

Objectives: We studied the experimental use of Seprafilm, a sheet-like filmform of hyaluronic acid, in the repair of subtotal perforations of the tympanic membrane (TM), and the durability of such repairs.Method: The research was conducted on 42 Sprague–Dawley rats. Right TMs of all rats were subtotally and thermally perforated in the same fashion. Seprafilm was applied to the perforated TMs of the 21 rats comprising the experiment group. The perforated TMs of the remaining 21rats, the control group, were left to heal spontaneously.Results: The mean healing times were recorded as 7.8 ± 0.6 days for the Seprafilm group and 14.9 ± 1.1 days for the control group (p < 0.001).Conclusions: These results suggest that Seprafilm may be a possible first choice (before thedecision to operate) in subtotal perforations, considering its easy application and patient follow-up.

scholarly journals MTA and calcium hydroxide for pulp capping

2005 ◽  
Vol 13 (2) ◽  
pp. 126-130 ◽  
Author(s):  
Alexandra Mussolino de Queiroz ◽  
Sada Assed ◽  
Mario Roberto Leonardo ◽  
Paulo Nelson-Filho ◽  
Léa Assed Bezerra da Silva
Keyword(s):  
Calcium Hydroxide ◽  
Mineral Trioxide Aggregate ◽  
Inflammatory Cells ◽  
Direct Pulp Capping ◽  
Water Control ◽  
Pulp Tissue ◽  
Pulp Capping ◽  
Tissue Changes ◽  
Maxilla And Mandible ◽  
Direct Capping

This study evaluated the biocompatibility of mineral trioxide aggregate (MTA) after direct capping of exposed pulp tissue in dog's teeth. Class I cavities were prepared in 26 teeth from 3 adult dogs. MTA was applied over the exposed pulp in 13 teeth and paste of calcium hydroxide plus distilled water (control) was applied in the remaining 13 teeth. After 90 days, the animals were killed; the maxilla and mandible were dissected and sectioned to obtain individual roots. The samples were processed histologically. The pulp and periapical response observed with the use of MTA was similar to that of calcium hydroxide paste. In all specimens, there was a dentin bridge obliterating the exposure, an intact odontoblastic layer, no inflammatory cells, normal connective pulp tissue, normal apical and periapical regions and no bone tissue changes. Similar to calcium hydroxide, MTA presented excellent response when used for direct pulp capping.

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