scholarly journals Stem cell factor in gingival crevicular fluid in periodontal health and disease

2010 ◽  
Vol 4 (2) ◽  
pp. 281-287 ◽  
Author(s):  
Sittisak Honsawek ◽  
Wannee Unwerawattana
Keyword(s):  
Stem Cell ◽  
G Protein ◽  
Stem Cell Factor ◽  
Gingival Crevicular Fluid ◽  
Periodontal Health ◽  
Group Iii ◽  
Group I ◽  
Health And Disease ◽  
Group Ii ◽  
Crevicular Fluid

Abstract Background: Stem cell factor is a pleiotropic cytokine produced by several cell types including fibroblasts, bone marrow stromal cells, mast cells, and endothelial cells. In addition, stem cell factor is an important hematopoietic growth factor, which binds to and activates the ligand for the tyrosine kinase-type receptor c-kit. Objectives: Analyze concentration of stem cell factor within gingival crevicular fluid (GCF) in both periodontal health and disease and to determine the correlation of stem cell factor in GCF and inflammatory status of periodontal tissues. Materials and methods: Forty-five subjects (aged 24 to 75 years) were classified into the following three groups according to their periodontal tissue status as group I (clinically healthy gingiva with no loss of attachment), group II (gingivitis with no attachment loss), and group III (periodontitis). GCF samples collected from each patient were examined for stem cell factor level using enzyme-linked immunosorbant assay. Results: The maximum level of stem cell factor in GCF was obtained for group III (71.8±7.8 pg/g protein), and the lowest mean stem cell factor concentration in GCF was observed for group I (22.1±7.3 pg/g protein). The GCF stem cell factor level of patients in group III was statistically higher than that in group II (p <0.04) and group I (p <0.001). In addition, the mean GCF levels of stem cell factor in group II (48.1±7.5 pg/g protein) were significantly higher than those in group I (p <0.02). There was a positive correlation between stem cell factor in GCF and gingival inflammation index (r=0.59, p <0.001) Conclusion: GCF levels of stem cell factor increased in parallel with the severity of periodontal disease. Its levels in GCF could be potentially useful as a biochemical marker of periodontal inflammation and the host response.

scholarly journals Gingival crevicular fluid level of soluble triggering recep-tor expressed on myeloid cells-1(strem-1) in periodontal health and disease: a case-control study

2017 ◽  
Vol 5 (2) ◽  
pp. 83
Author(s):  
Surabhi Gigras ◽  
Sudhir R Patil ◽  
Veena HR ◽  
Sneha Dani
Keyword(s):  
Chronic Periodontitis ◽  
Myeloid Cells ◽  
Cell Surface Receptor ◽  
Periodontal Health ◽  
Group Iii ◽  
Root Planing ◽  
Group I ◽  
Health And Disease ◽  
Group Ii ◽  
Crevicular Fluid

Background: The Triggering Receptor Expressed On Myeloid Cells-1(TREM-1) is a cell-surface receptor of the immunoglobulin superfamily and found to be involved in the amplification of the inflammatory response to various microbial infections, including periodontal diseases.Objectives: The present study was designed to examine gingivalcrevicular fluid(GCF) levels of soluble TREM-1 (sTREM-1) levels in periodontal health and disease as well as evaluate the effect of scaling and/or root planing on the same.Methods: Based on gingival index, probing pocket depth, clinical attachment level, and radiologic parameters (bone loss), 45 subjects were initially divided into three groups- Group, I (Periodontally healthy), Group II (Gingivitis) and Group III (Chronic Periodontitis).From each of the subjects, GCF sample was collected at baseline and scaling and/or root planing was instituted in group II and group III patients. GCF samples were subsequently collected at eight-week interval. Levels of sTREM-1 in collected GCF samples were estimated using enzyme-linked immunosorbent assay.Results: The lowest GCF levels of sTREM-1 were found in periodontal health (69.50±1.8pg/ml) followed by gingivitis (257.17±79pg/ml) and chronic periodontitis (3658.14±55pg/ml) in increasing order, suggesting that levels of sTREM-1 in crevicular fluid increased with the severity of periodontal disease. sTREM-1 levels decreased significantly from baseline to the end of 8 weeks following non-surgical periodontal therapy.Conclusion: Increased GCF levels of sTREM-1 from periodontal health to disease strengthen its association with periodontal status.

scholarly journals Effect of Periodontal Therapy on Crevicular Fluid Interleukin-18 Level in Periodontal Health and Disease in Central Maharashtra (India) Population

2017 ◽  
Vol 18 (11) ◽  
pp. 1085-1089 ◽  
Author(s):  
Monica J Mahajani ◽  
Varsha A Jadhao ◽  
Pooja S Wankhade ◽  
Emmanuel Samson ◽  
Vishwas D Acharya ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Gingival Crevicular Fluid ◽  
Periodontal Therapy ◽  
Interleukin 18 ◽  
Periodontal Health ◽  
Group I ◽  
Nonsurgical Periodontal Therapy ◽  
Health And Disease ◽  
Group Ii ◽  
Crevicular Fluid

ABSTRACT Aim The incidence and progression of the periodontal disease depend on periodontal microflora and the multifaceted response of the host, and these interactions are mediated by cytokines and chemokines. Interleukin-18 (IL-18) is a proinflammatory cytokine of the IL-1 superfamily. The aim of the present study was the assessment of the periodontal therapy in IL-18 level in periodontal disease and health. Materials and methods Based on clinical attachment loss (CAL), probing pocket depth (PPD), gingival index (GI), and plaque index (PI) patients were divided into three groups: Group I with healthy patients, group II with chronic periodontitis, and group III with posttreatment patients having periodontitis. Results Mean PI, PPD, CAL, and gingival crevicular fluid (GCF) volume were significantly higher in groups II and III compared with group I. However, there were no significant differences between GI in groups I, II, and III. The total amount of IL-18 in GCF was significantly higher in group II when compared with groups I and III (p < 0.05). Conclusion The present study confirmed that the IL-18 level in GCF was lower in healthy patients, higher in periodontally involved patients, and reduced at baseline, 3 and 6 weeks after nonsurgical periodontal therapy. Clinical significance The IL-18 might be hypothetically beneficial in distinguishing health from disease and monitoring periodontal disease activity. How to cite this article Mahajani MJ, Jadhao VA, Wankhade PS, Samson E, Acharya VD, Tekale PD. Effect of Periodontal Therapy on Crevicular Fluid Interleukin-18 Level in Periodontal Health and Disease in Central Maharashtra (India) Population. J Contemp Dent Pract 2017;18(11):1085-1089.

scholarly journals An Evaluation and Correlation of Leptin in Gingival Crevicular Fluid and Serum in Health, Gingivitis and Periodontitis

2012 ◽  
Vol 1 (2) ◽  
pp. 93-97
Author(s):  
Vinay Vadvadgi ◽  
Neeta Padmawar
Keyword(s):  
Periodontal Disease ◽  
Gingival Crevicular Fluid ◽  
Gingival Tissue ◽  
Enzyme Linked Immunosorbent Assay ◽  
Group Iii ◽  
Group I ◽  
The Mean ◽  
Group Ii ◽  
Crevicular Fluid ◽  
Mean Concentration

ABSTRACT Background and objective Plasma leptin is associated in patients with inflammatory diseases. A high concentration of leptin is associated with healthy gingival tissue. The purpose of this study was to assess the concentration of human leptin in gingival crevicular fluid (GCF) and serum within healthy and diseased gingiva, further to explore the possibility of using the levels of leptin in GCF and serum as a biochemical marker of periodontal disease progression. Materials and methods Ninety subjects were selected with age (30-39 years) and sex (15 males and 15 females) matched, to eliminate age and sex as confounders. The subjects were divided into three groups consisting of 30 subjects in each group based on the clinical and radiological parameters; healthy (group I), gingivitis (group II), periodontitis (group III), from whom the GCF samples were collected with Periopaper GCF collection strips (Proflow, Amityville, NY, USA) for 30 seconds and blood samples with 20-gauge needle syringe respectively. Leptin concentration was determined from individual GCF and serum samples by enzyme-linked immunosorbent assay (ELISA). Results The highest mean leptin concentration in GCF was observed in group I (2,664.30 pg/ml ± 324.73) and least mean leptin concentration was obtained in group III (1,309.43 pg/ml ± 202.45). The mean concentration of group II (1,639.43 pg/ml ± 344.46) was intermediate between the highest and lowest values. In contrast, the highest mean leptin concentration in serum was obtained for group III (12,086.57 pg/ml ± 1,698.23) and least mean leptin concentration was obtained for group I (8,715.09 pg/ml ± 1,649.19). The mean concentration of the group II (10,694.01 pg/ml ± 1,777.72) were intermediate between the highest and lowest values. Conclusion The results indicated a statistically significant decrease in the GCF leptin concentration and increase in serum leptin concentration as the periodontal disease progressed. How to cite this article Vadvadgi VH, Saini R, Padmawar N. An Evaluation and Correlation of Leptin in Gingival Crevicular Fluid and Serum in Health, Gingivitis and Periodontitis. Int J Experiment Dent Sci 2012;1(2):93-97.

Gingival Crevicular Fluid Levels of Leukotriene B4in Periodontal Health and Disease

2007 ◽  
Vol 78 (12) ◽  
pp. 2325-2330 ◽  
Author(s):  
A.R. Pradeep ◽  
S.G. Manjunath ◽  
Pradeep Patel Swati ◽  
Chowdhry Shikha ◽  
Pai B. Sujatha
Keyword(s):  
Gingival Crevicular Fluid ◽  
Periodontal Health ◽  
Health And Disease ◽  
Crevicular Fluid ◽  
Fluid Levels

scholarly journals Gingival Crevicular Fluid and Serum Cystatin C Levels in Periodontal Health and Disease

2012 ◽  
Vol 32 (2) ◽  
pp. 101-107 ◽  
Author(s):  
Anuj Sharma ◽  
A. R. Pradeep ◽  
N. M. Raghavendra ◽  
P. Arjun ◽  
Rahul Kathariya
Keyword(s):  
Cystatin C ◽  
Chronic Periodontitis ◽  
Gingival Crevicular Fluid ◽  
Therapy Group ◽  
Periodontal Diseases ◽  
Periodontal Therapy ◽  
Periodontal Health ◽  
Healthy Group ◽  
Health And Disease ◽  
Crevicular Fluid

Cystatin C (CSTC) is an inhibitor of cysteine proteinases and could play a protective and regulatory role under inflammatory conditions. The present study was designed to assess the concentration of CSTC in gingival crevicular fluid (GCF) and serum, to find out their association if any, in periodontal health and disease. 30 subjects were selected divided into 3 groups consisting of 10 subjects in each group based on clinical parameters: periodontally healthy group, gingivitis group and chronic periodontitis group, while, chronic periodontitis group after 8 weeks of the treatment (scaling and root planing) constituted after periodontal therapy group. GCF and serum samples were collected from all subjects to estimate the levels of CSTC by ELISA. The mean CSTC concentration in GCF and serum was observed to be the highest in periodontitis group and lowest in periodontally healthy group with intermediate concentration in gingivitis group and after periodontal therapy group. CSTC concentration in GCF and serum increased proportionally with the severity of periodontal disease (from health to periodontitis group) and decreased after treatment. This suggests that CSTC increases with disease progression to prevent further periodontal degeneration and decreases after treatment due to bone metabolic homeostasis. Further, longitudinal prospective studies involving larger population are needed to confirm the findings of present study and to better understand the role of CSTC in the pathogenesis of periodontal diseases.

Quantitative analysis of MRP-8 in gingival crevicular fluid in periodontal health and disease using microbore HPLC

2001 ◽  
Vol 28 (12) ◽  
pp. 1172-1177 ◽  
Author(s):  
Fionnuala T. Lundy ◽  
Rod Chalk ◽  
Philip-John Lamey ◽  
Christopher Shaw ◽  
Gerard J. Linden
Keyword(s):  
Quantitative Analysis ◽  
Gingival Crevicular Fluid ◽  
Periodontal Health ◽  
Health And Disease ◽  
Crevicular Fluid
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