scholarly journals GENOME SIZE DETERMINATION OF CUCUMBER (CUCUMIS SATIVUS), HONEYDEW (CUCUMIS MELO INODORUS) AND ROCK MELON (CUCUMIS MELO CANTALUPENSIS) VIA FLOW CYTOMETRY

2021 ◽  
Vol 5 (1) ◽  
pp. 14-16
Author(s):  
Raden Muhamad Imaduddin Yumni ◽  
Mohd Fauzihan Karim ◽  
Mohd Razik Midin
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Cucumis Melo ◽  
As Species ◽  
External Reference ◽  
The Family ◽  
Cucumis Species ◽  
Fcm Analysis ◽  
Interspecific And Intraspecific Variation

The family of Cucurbitaceae consists of species with economical and nutritional value. Morphologically, there are only few differences between Cucumis species. The interspecific and intraspecific variation in the genome size of the Cucumis species are not discovered yet. Due to this, this study aims to determine the genome size of C. sativus, C. melo inodorus and C. melo cantalupensis using flow cytometry (FCM) method. Nuclei suspension of selected Cucumis species were extracted using LBO1 lysis buffer by manual chopping technique and stained by propidium iodide priot to FCM analysis. Genome size of C. sativus, C. melo inodorus (Honeydew) and C. melo cantalupensis (Rockmelon) were determined by using Glycine max (Soybean) as an external reference standard (2C = 2.5 pg). This study found that the genome size of C. sativus, C. melo inodorus and C. melo cantalupensis estimated to be 2.83 pg, 3.00 pg and 3.47 pg respectively. The genome size data obtained from this study can be used in future genome studies as well as species characterization.

scholarly journals Nuclear Genome Size Determination Of Christia Vespertilionis Via Flow Cytometry

2020 ◽  
Vol 4 (2) ◽  
pp. 72-75
Author(s):  
Mohd Razik Midin ◽  
Muhammad Irfan Fikri ◽  
Siti Sarah Zailani
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Nuclear Genome ◽  
Size Determination ◽  
Size Estimation ◽  
Butterfly Wing ◽  
External Reference ◽  
Fcm Analysis ◽  
Suspension Preparation

AbstractChristia vespertilionis (butterfly wing plant) is an ornamental plant originated from South East Asia with reported usage in traditional medicine practice and potential as an anticancer and antitumor. This research aims to estimate the genome size of C. vespertilionis via flow cytometry (FCM) method. The research was conducted with the optimisation of nuclear suspension preparation followed by the genome size estimation. Two chopping techniques [manual chopping (MC) and BDTM Medimachine (MM)] and two lysis buffers (Otto and LBO1) were tested. Otto buffer with manual chopping was found to be the most suitable method, generated fine DNA peak with minimum debris background, and coefficient of variation (CV) value less than 3%. Five replicates of the FCM analysis were made for the genome size determination. The estimated genome size of C. vespertilionis was found to be 3.22 pg by using Glycine max cv. Polanka (2C=2.5pg) as an external reference standard. Further comparison with other Christia species was not possible due to the lack of data on genome size. The genome size data of C. vespertilionis can be useful for future morphology and genetics studies of Christia species.

scholarly journals Application of flow cytometry and cell sorting to megakaryocytopoiesis

Blood ◽  
1979 ◽  
Vol 53 (4) ◽  
pp. 732-745 ◽  
Author(s):  
A Nakeff ◽  
F Valeriote ◽  
JW Gray ◽  
RJ Grabske
Keyword(s):  
Flow Cytometry ◽  
Cell Sorting ◽  
Significant Advance ◽  
Glass Slides ◽  
Acetylcholinesterase Staining ◽  
Fcm Analysis ◽  
Cell Fractions ◽  
Kinetics Of ◽  
Supravital Staining

Abstract We have employed flow cytometry (FCM) and cell sorting to quantitate and study megakaryocytes in mouse and rat femoral marrow following their 20- to 30-fold concentration by centrifugal elutriation (CE). This enrichment of megakaryocytes permitted the first determination of their DNA-related fluorescence by FCM analysis following DNA staining. Fluorescence distributions of CE-enriched cell fractions following supravital staining with Hoechst 33342 were similar to those following chromomycin A3 staining of ethanol-fixed cells. Microscopic examination of cells sorted onto glass slides on the basis of their DNA-related fluorescence following supravital staining together with specific acetylcholinesterase staining for megakaryocytes indicated that megakaryocytes generally increased in cell size with increasing DNA content. This technologic application represents a significant advance in the study of megakaryocytopoiesis, since the kinetics of either the normal or perturbed population can now be studied rapidly and quantitatively.

scholarly journals Determination of genome size variations among different date palm cultivars (Phoenix dactylifera L.) by flow cytometry

3 Biotech ◽  
2019 ◽  
Vol 9 (12) ◽  
Author(s):  
Tahira Jatt ◽  
Moon-Sub Lee ◽  
A. Lane Rayburn ◽  
Mushtaque Ahmed Jatoi ◽  
Abdul Aziz Mirani
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Date Palm ◽  
Phoenix Dactylifera ◽  
Phoenix Dactylifera L

scholarly journals Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses

Caryologia ◽  
2017 ◽  
Vol 71 (1) ◽  
pp. 35-44 ◽  
Author(s):  
Mohd Razik Midin ◽  
Mohd Shukor Nordin ◽  
Maria Madon ◽  
Mohd Nazre Saleh ◽  
Hoe-Han Goh ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Chromosome Number ◽  
Genome Size ◽  
Garcinia Mangostana

Determination of genome size and chromosome ploidy of selected taxa from Prunus armeniaca by flow cytometry

2020 ◽  
Vol 261 ◽  
pp. 108987 ◽  
Author(s):  
Wenwen Li ◽  
Liqiang Liu ◽  
Yanan Wang ◽  
Guoquan Fan ◽  
Shikui Zhang ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Prunus Armeniaca

scholarly journals Application of flow cytometry and cell sorting to megakaryocytopoiesis

Blood ◽  
1979 ◽  
Vol 53 (4) ◽  
pp. 732-745 ◽  
Author(s):  
A Nakeff ◽  
F Valeriote ◽  
JW Gray ◽  
RJ Grabske
Keyword(s):  
Flow Cytometry ◽  
Cell Sorting ◽  
Significant Advance ◽  
Glass Slides ◽  
Acetylcholinesterase Staining ◽  
Fcm Analysis ◽  
Cell Fractions ◽  
Kinetics Of ◽  
Supravital Staining

We have employed flow cytometry (FCM) and cell sorting to quantitate and study megakaryocytes in mouse and rat femoral marrow following their 20- to 30-fold concentration by centrifugal elutriation (CE). This enrichment of megakaryocytes permitted the first determination of their DNA-related fluorescence by FCM analysis following DNA staining. Fluorescence distributions of CE-enriched cell fractions following supravital staining with Hoechst 33342 were similar to those following chromomycin A3 staining of ethanol-fixed cells. Microscopic examination of cells sorted onto glass slides on the basis of their DNA-related fluorescence following supravital staining together with specific acetylcholinesterase staining for megakaryocytes indicated that megakaryocytes generally increased in cell size with increasing DNA content. This technologic application represents a significant advance in the study of megakaryocytopoiesis, since the kinetics of either the normal or perturbed population can now be studied rapidly and quantitatively.

scholarly journals Cytogenetic relationships within the Maghrebian clade of Festuca subgen. Schedonorus (Poaceae), using flow cytometry and FISH

2017 ◽  
Vol 74 (1) ◽  
pp. 052 ◽  
Author(s):  
David Ezquerro-López ◽  
David Kopecký ◽  
Luis Á. Inda
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Fluorescent In Situ Hybridization ◽  
Genome Duplication ◽  
Size Estimation ◽  
Ploidy Levels ◽  
New Information ◽  
Shed Light

Festuca subgen. Schedonorus is a group of broad-leaved fescues, which can be divided into two clades: European and Maghrebian. We employed fluorescent in situ hybridization —FISH— with probes specific for 5S and 35S ribosomal DNA and genome size estimation using flow cytometry to shed light on the determination of possible parental genomes of polyploid species of the Maghrebian clade. Our results indicate that octoploid F. arundinacea subsp. atlantigena probably originated from crossing of the tetraploids F. arundinacea subsp. fenas —2n = 4x = 28— and F. mairei —2n = 4x = 28— followed by whole genome duplication. However, a large reconstruction of karyotype and genome downsizing has been revealed. Similarly, hexaploid F. arundinacea subsp. corsica presumably resulted from the interspecific hybridization of the diploid F. pratensis and tetraploid F. arundinacea subsp. fenas. Several scenarios on the origin of decaploid F. arundinacea var. letourneuxiana are discussed. This study contributed to our knowledge on the phylogeny of broad-leaved fescues and provided new information on the karyotypes —chromosome numbers, ploidy levels and numbers and positions of rDNA loci— using FISH and genome size estimations using flow cytometry in selected taxa of this important grass genus.

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