scholarly journals Concentrations of bone morphogenetic protein-15 (BMP-15) and growth differentiation factor-9 (GDF-9) in follicular cysts, mono - and polyoocyte follicles in gilts

2014 ◽  
Vol 64 (1) ◽  
pp. 24-32 ◽  
Author(s):  
Tomasz Stankiewicz ◽  
Barbara Błaszczyk
Keyword(s):  
Analysis Of Variance ◽  
Follicular Fluid ◽  
Ovarian Follicles ◽  
Cystic Fluid ◽  
Single Cyst ◽  
Morphogenetic Protein ◽  
Growth Differentiation Factor 9 ◽  
Bone Morphogenetic Protein 15 ◽  
Range Test ◽  
Follicular Cysts

Abstract The objective of the study was to determine the concentration of BMP-15 and GDF-9 in the fluid of follicular cysts and ovarian follicles, and to compare their concentrations in mono- and polyoocyte follicles in gilts. The study involved two experiments conducted on the ovaries collected post-slaughter from gilts (7-8 months old). The first experiment covered 31 follicular single cyst gilts (15-25 mm in diameter) and 41 gilts without cysts. Follicular fluid from follicles of 8-10 mm in diameter (n=41) and 5-8 mm in diameter (n=41), and cystic fluid (n=31) were collected for analysis. The second experiment involved collecting follicular fluid from poly- (n=19) and monooocyte (n=22) follicles. The concentration of BMP-15 and GDF-9 was then determined in the samples using specimen-specific ELISA kits. The differences in the concentration of these factors were calculated by means of analysis of variance and a posthoc test. Duncan’s multiple range test was used to verify the significance of differences at P<0.05 and P<0.01. In addition, correlations between the factors were calculated. BMP-15 and GDF-9 levels in the cystic fluid were significantly higher than those in the follicular fluid (P<0.01). However, no differences were observed between various size follicles or between mono- and polyoocyte follicles. BMP-15 and GDF-9 concentrations were found to be positively correlated (P<0.01). Differences in BMP-15 and GDF-9 concentrations in ovarian follicles and follicular cysts, as evidenced by our study, indicate that these factors may be related to folliculogenesis disorders in gilts. What is more, the number of oocytes in ovarian follicles does not influence the intrafollicular concentration of BMP-15 and GDF-9.

scholarly journals The relationships between transforming growth factors <i>β</i> and free thyroxine and progesterone in the ovarian cysts, preovulatory follicles, and the serum of sows

2017 ◽  
Vol 60 (2) ◽  
pp. 131-136 ◽  
Author(s):  
Tomasz Stankiewicz
Keyword(s):  
Cyst Formation ◽  
Free Thyroxine ◽  
Transforming Growth Factors ◽  
Cystic Fluid ◽  
Morphogenetic Protein ◽  
Preovulatory Follicles ◽  
Growth Differentiation Factor 9 ◽  
Bone Morphogenetic Protein 15 ◽  
Insight Into

Abstract. The aim of the study was to determine the relationships between bone morphogenetic protein 15 (BMP-15) and growth differentiation factor 9 (GDF-9) concentrations and free thyroxine (FT4) and progesterone (P4) concentrations in follicular cysts, preovulatory follicles, and the serum of sows (cyst-bearing (n = 26) and non-cyst-bearing (n =  26)). FT4 and P4 concentrations were higher in the cystic fluid than in the fluid of preovulatory follicles (p <  0.01 and p <  0.05 respectively). BMP-15 and GDF-9 concentrations were higher in the serum of cyst-bearing sows than non-cyst-bearing sows (p <  0.05) and higher in the cystic fluid than in the follicular fluid (p <  0.05). In the cysts and preovulatory follicles, GDF-9 concentration was higher than in serum (p <  0.01). FT4 concentration in the serum of cystic sows was correlated with BMP-15 (r =  0.50, p <  0.05) and GDF-9 (r =  0.62, p <  0.01) concentrations in serum. In the serum of non-cyst-bearing sows, a positive correlation between P4 concentration and BMP-15 concentration (r =  0.60, p <  0.01) was detected. These data will help provide insight into the role of BMP-15, GDF-9, FT4, and P4 during cyst formation in sows.

Relationship between the concentration of bone morphogenetic protein-15 (BMP-15) and growth differentiation factor-9 (GDF-9) in pre-ovulatory follicles, ovarian cysts and serum in sows

2016 ◽  
Vol 56 (1) ◽  
pp. 141 ◽  
Author(s):  
Tomasz Stankiewicz ◽  
Barbara Błaszczyk
Keyword(s):  
Bone Morphogenetic Protein ◽  
Follicular Fluid ◽  
Cyst Fluid ◽  
Ovarian Cysts ◽  
Positive Correlation ◽  
Differentiation Factor ◽  
Morphogenetic Protein ◽  
Growth Differentiation Factor 9 ◽  
Bone Morphogenetic Protein 15 ◽  
Ovulatory Follicles

The aim of the study was to compare and assess the relationship between peripheral and ovarian bone morphogenetic protein-15 (BMP-15) and growth differentiation factor-9 (GDF-9) concentration in cysts-bearing and non-cysts-bearing sows. The research involved multiparous sows (cysts-bearing sows, n = 20; non-cysts-bearing sows, n = 26). Serum, cystic follicular fluid or fluid from the ovulatory follicles was collected from each of the sows at the time of the slaughter. The collected serum and cystic/follicular fluid samples were tested for BMP-15 and GDF-9 concentrations. BMP-15 and GDF-9 concentrations in fluid from ovarian cysts were significantly higher than in the follicular fluid (P < 0.01). The concentration of BMP-15 and GDF-9 in the serum of sows with cysts was also higher than in ovulating sows (P < 0.05 and P < 0.01, respectively). The concentration of BMP-15 in serum was positively correlated with the concentration of said protein in pre-ovulatory follicles and cysts (r = 0.70; P < 0.01 and r = 0.50; P < 0.05, respectively). A positive correlation was also found between the concentration of serum GDF-9 and cyst GDF-9 (r = 0.55; P < 0.05). Moreover, another positive correlation was detected between the BMP-15 and GDF-9 concentrations, with the highest correlation coefficient recorded in the cyst fluid (r = 0.87; P < 0.01). This study for the first time demonstrates the presence of BMP-15 and GDF-9 in the cyst fluid in sows and determines a positive correlation between the concentration of the above factors in the follicular/cystic fluid and serum. Therefore, it is possible that the determination of BMP-15 and GDF-9 serum concentration may assist diagnosing ovarian cysts in sows. However, it is suggested that further studies involving a more in-depth analysis of the role of BMP-15 and GDF-9 in the pathogenesis of ovarian cysts in pigs are needed.

scholarly journals Expression Profiles of the Progesterone Receptor, Cyclooxygenase-2, Growth Differentiation Factor 9, and Bone Morphogenetic Protein 15 Transcripts in the Canine Oviducts during the Oestrous Cycle

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 454
Author(s):  
Jaime Palomino ◽  
Javiera Flores ◽  
Georges Ramirez ◽  
Victor H. Parraguez ◽  
Monica De los Reyes
Keyword(s):  
Gene Expression ◽  
Bone Morphogenetic Protein ◽  
Cyclooxygenase 2 ◽  
Oestrous Cycle ◽  
Progesterone Concentration ◽  
Plasma Progesterone ◽  
Morphogenetic Protein ◽  
Growth Differentiation Factor 9 ◽  
Bone Morphogenetic Protein 15 ◽  
Cox 2

The gene expression in the canine oviduct, where oocyte maturation, fertilization, and early embryonic development occur, is still elusive. This study determined the oviductal expression of (PR), cyclooxygenase-2 (COX-2), growth differentiation factor 9 (GDF-9), and bone morphogenetic protein 15 (BMP-15) during the canine oestrous cycle. Samples were collected from bitches at anoestrus (9), proestrus (7), oestrus (8), and dioestrus (11), after routine ovariohysterectomy and the ovarian surface structures and plasma progesterone concentration evaluated the physiological status of each donor. The oviductal cells were isolated and pooled. Total RNA was isolated, and gene expression was assessed by qPCR followed by analysis using the t-test and ANOVA. The PR mRNA increased (P < 0.05) from the anoestrus to dioestrus with the plasma progesterone concentration (r = 0.8). COX-2 mRNA expression was low in the anoestrus and proestrus, and negligible in the oestrus, while it was around 10-fold higher (P < 0.05) in the dioestrus. The GDF-9 mRNA was expressed during all phases of the oestrous cycle and was most abundant (P < 0.05) during oestrus phase. The BMP-15 mRNA decreased (P < 0.05) in the anoestrus and proestrus phases. Thus, the transcripts were differentially expressed in a stage-dependent manner, suggesting the importance of oestrous cycle regulation for successful reproduction in dogs.

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