scholarly journals First Occurence of African Swine Fever in Serbia

2019 ◽  
Vol 69 (4) ◽  
pp. 443-449
Author(s):  
Vesna Milićević ◽  
Branislav Kureljušić ◽  
Jelena Maksimović Zorić ◽  
Božidar Savić ◽  
Slobodan Stanojević ◽  
...  
Keyword(s):  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Classical Swine Fever ◽  
Surveillance Program ◽  
Reference Laboratory ◽  
First Case ◽  
Record Data ◽  
Gross Lesions ◽  
Virus Isolates ◽  
The Eu

Abstract Until July 30th, 2019 when the first case of African swine fever (ASF) was confirmed, Serbia was a country free from ASF. After the owner reported atypical illness and death of a sow, the local veterinarian submitted the organ samples to the National Reference Laboratory for Classical Swine Fever (CSF) and African Swine Fever within the Institute of Veterinary Medicine of Serbia in Belgrade. Observed gross lesions included splenomegaly, serous edema of the wall of the gallbladder and hemorrhages in the enlarged portal lymph nodes, petechial hemorrhages on the kidney and epicardium, and petechial and echymotic hemorrhages on the mucosa of the urinary bladder. Results of real-time PCR confirmed that the cause of illness and death of the swine was African swine fever virus. The samples were sent for confirmation to the EU Reference Laboratory where it was confirmed that Serbian domestic pig virus isolates based on p72 belong to genotype II. In total, 270 pigs from 18 affected holdings were killed in the infected zones. According to the on-record data, mortality was 6.89%, whereas lethality reached 64.5%. Currently, an extensive surveillance program is being conducted, aiming to force passive surveillance. ASF in wild boar has not been confirmed so far.

African swine fever virus introduction into the EU in 2014: Experience of Latvia

2016 ◽  
Vol 105 ◽  
pp. 28-30 ◽  
Author(s):  
Edvīns Oļševskis ◽  
Vittorio Guberti ◽  
Mārtiņš Seržants ◽  
Jørgen Westergaard ◽  
Carmina Gallardo ◽  
...  
Keyword(s):  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
The Eu

scholarly journals CLONING OF GENES ENCODING TRANSMEMBRANE PROTEINS AND PROTEINS RESPONSIBLE FOR AFRICAN SWINE FEVER VIRUS VIRULENCE

2018 ◽  
pp. 8-12
Author(s):  
Ali Mazloum ◽  
N. G. Zinyakov ◽  
A. S. Igolkin ◽  
N. N. Vlasova
Keyword(s):  
Recombinant Protein ◽  
Clone Library ◽  
African Swine Fever Virus ◽  
Clinical Manifestations ◽  
Transmembrane Proteins ◽  
African Swine Fever ◽  
Virus Infectivity ◽  
Reference Laboratory ◽  
Genes Encoding

Results of cloning X69R, A179L, E248R, I215L and DP96R genes of ASF virus Krasnodar 07/17 isolate and analysis of their nucleotide sequences are presented. Obtained clones were added to the previously constructed clone library comprising clones of 8 genes of Krasnodar 06/12 isolate. Clones containing X69R, A179L, E248R, I215L and DP96R genes of ASF virus Krasnodar 07/17 isolate will be used for recombinant protein obtaining and testing for their effect on in vitro virus reproduction and their role in the virus infectivity, level of clinical manifestations and virulence. Prokaryotic vector, pJET1.2/ blunt, was used. Thus, the clone library available at the FGBI “ARRIAH” Reference Laboratory for African swine fever was supplemented by pJET1.2-X69R, pJET1.2-A179L, pJET1.2-E248R, pJET1.2-I215L and pJET1.2-DP96R plasmid constructions containing 5 genes of ASF virus Krasnodar 07/17 isolate. Proportion of cloned virus genes was 3.01% of Krasnodar 07/17 isolate genome, hence, total amount of the clone library has reached 7.82%.

scholarly journals African swine fever: A permanent threat to Indian pigs

2020 ◽  
Vol 13 (10) ◽  
pp. 2275-2285
Author(s):  
Sharanagouda S. Patil ◽  
Kuralayanapalya Puttahonnappa Suresh ◽  
Vikram Vashist ◽  
Awadhesh Prajapati ◽  
Bramhadev Pattnaik ◽  
...  
Keyword(s):  
Russian Federation ◽  
Clinical Symptoms ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Classical Swine Fever ◽  
Viral Diseases ◽  
Arunachal Pradesh ◽  
First Report ◽  
The North ◽  
North Eastern

India has 9 million pigs, of which 45% are in the North eastern (NE) states of India. Viral diseases affecting pigs are a major concern of mortality causing huge loss to the pig farmers. One such disease is African swine fever (ASF) that has already knocked the porous borders of NE states of India. ASF is a highly contagious devastating disease of pigs and wild boars causing 100% mortality. The causative agent African swine fever virus (ASFV) belongs to the genus Asfivirus, family Asfarviridae. Pig is the only species affected by this virus. Soft ticks (Ornithodoros genus) are shown to be reservoir and transmission vectors of ASFV. Transmission is very rapid and quickly engulfs the entire pig population. It is very difficult to differentiate classical swine fever from ASF since clinical symptoms overlap. Infected and in contact pigs should be culled immediately and buried deep, and sheds and premises be disinfected to control the disease. There is no vaccine available commercially. Since its first report in Kenya in 1921, the disease has been reported from the countries in Europe, Russian federation, China, and Myanmar. The disease is a threat to Indian pigs. OIE published the first report of ASF in India on May 21, 2020, wherein, a total of 3701 pigs died from 11 outbreaks (Morbidity - 38.45% and mortality - 33.89%) in Assam and Arunachal Pradesh states of India. ASF is non-zoonotic.

scholarly journals Salt inactivation of classical swine fever virus and African swine fever virus in porcine intestines confirms the existing in vitro casings model

2019 ◽  
Vol 238 ◽  
pp. 108424 ◽  
Author(s):  
Tinka Jelsma ◽  
Joris J. Wijnker ◽  
Bregtje Smid ◽  
Eline Verheij ◽  
Wim H.M. van der Poel ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Classical Swine Fever ◽  
Fever Virus

scholarly journals Molecular characterization and phylogenetic study of African swine fever virus isolates from recent outbreaks in Uganda (2010–2013)

2013 ◽  
Vol 10 (1) ◽  
Author(s):  
David Kalenzi Atuhaire ◽  
Mathias Afayoa ◽  
Sylvester Ochwo ◽  
Savannah Mwesigwa ◽  
Julius Boniface Okuni ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Phylogenetic Study ◽  
Virus Isolates

scholarly journals Rapid Extraction and Detection of African Swine Fever Virus DNA Based on Isothermal Recombinase Polymerase Amplification Assay

Viruses ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1731
Author(s):  
Arianna Ceruti ◽  
Rea Maja Kobialka ◽  
Judah Ssekitoleko ◽  
Julius Boniface Okuni ◽  
Sandra Blome ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Dna Isolation ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Recombinase Polymerase Amplification ◽  
Reference Laboratory ◽  
Analytical Sensitivity ◽  
Lysis Buffer

African swine fever virus (ASFV) is the causative agent of a deadly disease in pigs and is spread rapidly across borders. Samples collected from suspected cases must be sent to the reference laboratory for diagnosis using polymerase chain reaction (PCR). In this study, we aimed to develop a simple DNA isolation step and real-time recombinase polymerase amplification (RPA) assay for rapid detection of ASFV. RPA assay based on the p72 encoding B646L gene of ASFV was established. The assays limit of detection and cross-reactivity were investigated. Diagnostic performance was examined using 73 blood and serum samples. Two extraction approaches were tested: silica-column-based extraction method and simple non-purification DNA isolation (lysis buffer and heating, 70 °C for 20 min). All results were compared with well-established real-time PCR. In a field deployment during a disease outbreak event in Uganda, 20 whole blood samples were tested. The assay’s analytical sensitivity was 3.5 DNA copies of molecular standard per µL as determined by probit analysis on eight independent assay runs. The ASFV RPA assay only detected ASFV genotypes. Compared to real-time PCR, RPA diagnostic sensitivity and specificity were 100%. Using the heating/lysis buffer extraction procedure, ASFV-RPA revealed better tolerance to inhibitors than real-time PCR (97% and 38% positivity rate, respectively). In Uganda, infected animals were identified before the appearance of fever. The ASFV-RPA assay is shown to be as sensitive and specific as real-time PCR. Moreover, the combination of the simple extraction protocol allows its use at the point of need to improve control measures.

scholarly journals CLONING OF GENES ENCODING TRANSMEMBRANE PROTEINS AND PROTEINS RESPONSIBLE FOR AFRICAN SWINE FEVER VIRUS VIRULENCE

2018 ◽  
pp. 3-7 ◽  
Author(s):  
Ali Mazloum ◽  
N. G. Zinyakov ◽  
A. S. Igolkin ◽  
N. N. Vlasova
Keyword(s):  
Recombinant Protein ◽  
Clone Library ◽  
African Swine Fever Virus ◽  
Clinical Manifestations ◽  
Transmembrane Proteins ◽  
African Swine Fever ◽  
Virus Infectivity ◽  
Reference Laboratory ◽  
Genes Encoding

Results of cloning X69R, A179L, E248R, I215L and DP96R genes of ASF virus Krasnodar 07/17 isolate and analysis of their nucleotide sequences are presented. Obtained clones were added to the previously constructed clone library comprising clones of 8 genes of Krasnodar 06/12 isolate. Clones containing X69R, A179L, E248R, I215L and DP96R genes of ASF virus Krasnodar 07/17 isolate will be used for recombinant protein obtaining and testing for their effect on in vitro virus reproduction and their role in the virus infectivity, level of clinical manifestations and virulence. Prokaryotic vector, pJET1.2/ blunt, was used. Thus, the clone library available at the FGBI “ARRIAH” Reference Laboratory for African swine fever was supplemented by pJET1.2-X69R, pJET1.2-A179L, pJET1.2-E248R, pJET1.2-I215L and pJET1.2-DP96R plasmid constructions containing 5 genes of ASF virus Krasnodar 07/17 isolate. Proportion of cloned virus genes was 3.01% of Krasnodar 07/17 isolate genome, hence, total amount of the clone library has reached 7.82%.

scholarly journals EFFECTS OF MODERATELY VIRULENT AFRICAN SWINE FEVER VIRUS ON INTERLEUKIN-10 PRODUCTION

2019 ◽  
pp. 23-28 ◽  
Author(s):  
A. S. Pershin ◽  
I. V. Shevchenko ◽  
A. S. Igolkin ◽  
Ye. V. Aronova ◽  
N. N. Vlasova
Keyword(s):  
Immune Response ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Noticeable Effect ◽  
Specific Antibodies ◽  
Virulent Virus ◽  
Virus Isolates ◽  
Highly Virulent

A characteristic feature of African swine fever virus (ASFV) is the ability to escape from host immune response, affecting macrophages and replicating in them. Besides, ASFV - specific antibodies do not completely neutralize the virus. Cytokines are important factors for various viral infection pathologies. The virulence of ASFV isolates may depend on the capacity to regulate cytokine expression by macrophages. Thus, when comparing in vitro and in vivo cytokine production by macrophages, it was established that infection with low virulent virus isolates leads to an immune response with a predominance of cytokines involved in cellular immunity, such as INF-α and IL-12p40, as compared with infection with highly virulent isolates. The aim of this paper was to study the effect of African swine fever virus on the production of IL-10, a pleiotropic cytokine that inhibits synthesis of cytokines and shows a strong antiinflammatory effect. For this, 12 piglets were experimentally infected intramuscularly with a continuous cell culture-adapted ASFV isolate Vero25 at a dose of 10 HAdU per animal followed by control infection of surviving animals with the reference virus isolate Arm 07 at a dose of 1,000 HAdU per animal. Temperature measurements were taken and blood sampling to obtain serum was conducted during the experiment. IL-10 amount in blood sera was determined using Invitrogen test systems (Thermo Fisher, USA). A higher IL-10 level (15.8–173 pg/ml) was observed in blood sera of dead animals infected with a moderately virulent virus, as compared with surviving pigs (4–5 pg/ml). No correlation between the speed of appearance of specific antibodies and IL-10 serum levels has been established. No noticeable effect of the IL-10 serum level prior to infection on the survival rate of animals has been observed. Further studies are needed to establish a causal relationship, including study of the expression of various cytokines during infection with both low- and highly virulent virus isolates.

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