scholarly journals Plant Pillow Preparation for the Veggie Plant Growth System on the International Space Station

2020 ◽  
Vol 5 (1) ◽  
pp. 24-34 ◽  
Author(s):  
Gioia D. Massa ◽  
Gerard Newsham ◽  
Mary E. Hummerick ◽  
Robert C. Morrow ◽  
Raymond M. Wheeler

AbstractThe first Veggie plant growth chamber was installed on the International Space Station in 2014. Crop plants can be grown in Veggie using plant pillows, small rooting packets that contain substrate, fertilizer, and germination wicks along with attached seeds. The pillows were designed to interface with the Veggie root mat reservoir watering system to provide a capillary water column to growing plants. In preparation for flight, methods of arcillite substrate washing, autoclaving, and drying were established to reduce dust and to provide a dry sterile substrate. A controlled released fertilizer mixed into arcillite substrate provides nutrition for plant growth. Methods of seed surface sterilization were tested for both germination and microbial contamination, and the optimum methods were determined for candidate flight crops. Plant pillows were prepared for flight by cutting and inserting germination wicks, filling with the substrate/fertilizer mix, and sewing closed. Following pillow filling, seeds were attached to the wicks, and the pillows were packaged for flight. Pillow preparation methods have been successfully tested in the VEG-01 hardware validation tests on the International Space Station with ‘Outredgeous’ lettuce and ‘Profusion’ zinnia, and in the VEG-03 test, using ‘Outredgeous’ lettuce and ‘Tokyo bekana’ Chinese cabbage.

Plant Biology ◽  
2014 ◽  
Vol 16 (3) ◽  
pp. 528-538 ◽  
Author(s):  
A. -I. Kittang ◽  
T. -H. Iversen ◽  
K. R. Fossum ◽  
C. Mazars ◽  
E. Carnero-Diaz ◽  
...  

2020 ◽  
Author(s):  
Junya Zhang ◽  
Bárbara S.F. Müller ◽  
Kevin N. Tyre ◽  
Fang Bai ◽  
Ying Hu ◽  
...  

AbstractA biological life support system for spaceflight would capture carbon dioxide waste produced by living and working in space to generate useful organic compounds. Photosynthesis is the primary mechanism to fix carbon into organic molecules. Microalgae are highly efficient at converting light, water, and carbon dioxide into biomass, particularly under limiting, artificial light conditions that are a necessity in space photosynthetic production. Although there is great promise in developing algae for chemical or food production in space, most spaceflight algae growth studies have been conducted on solid agar-media to avoid handling liquids in microgravity. Here we report that breathable plastic tissue culture bags can support robust growth of Chlamydomonas reinhardtii in the Veggie plant growth chamber, which is used on the International Space Station to grow terrestrial plants. Live cultures can be stored for at least one month in the bags at room temperature. The gene set required for growth in these photobioreactors was tested through a short-wave ultraviolet light (UVC) mutagenesis and selection experiment with wild-type (CC-5082) and cw15 mutant (CC-1883) strains. Genome sequencing identified UVC-induced mutations, which were enriched for transversions and nonsynonymous mutations relative to natural variants among laboratory strains. Genes with mutations indicating positive selection were enriched for information processing genes related to DNA repair, RNA processing, translation, cytoskeletal motors, kinases, and ABC transporters. These data suggest modification of signal transduction and metabolite transport may be needed to improve growth rates in this spaceflight production system.


Author(s):  
Jonathan Lombardino ◽  
Swati Bijlani ◽  
Nitin K. Singh ◽  
Jason M. Wood ◽  
Richard Barker ◽  
...  

Sphingomonas is ubiquitous in nature, including the anthropogenically contaminated extreme environments. Members of the Sphingomonas genus have been identified as potential candidates for space biomining beyond earth.


2005 ◽  
Author(s):  
Danielle Paige Smith ◽  
Vicky E. Byrne ◽  
Cynthia Hudy ◽  
Mihriban Whitmore

2020 ◽  
Vol 91 (1) ◽  
pp. 41-45 ◽  
Author(s):  
Virginia. E. Wotring ◽  
LaRona K. Smith

INTRODUCTION: There are knowledge gaps in spaceflight pharmacology with insufficient in-flight data to inform future planning. This effort directly addressed in-mission medication use and also informed open questions regarding spaceflight-associated changes in pharmacokinetics (PK) and/or pharmacodynamics (PD).METHODS: An iOS application was designed to collect medication use information relevant for research from volunteer astronaut crewmembers: medication name, dose, dosing frequency, indication, perceived efficacy, and side effects. Leveraging the limited medication choices aboard allowed a streamlined questionnaire. There were 24 subjects approved for participation.RESULTS: Six crewmembers completed flight data collection and five completed ground data collection before NASA’s early study discontinuation. There were 5766 medication use entries, averaging 20.6 ± 8.4 entries per subject per flight week. Types of medications and their indications were similar to previous reports, with sleep disturbances and muscle/joint pain as primary drivers. Two subjects treated prolonged skin problems. Subjects also used the application in unanticipated ways: to note drug tolerance testing or medication holiday per research protocols, and to share data with flight surgeons. Subjects also provided usability feedback on application design and implementation.DISCUSSION: The volume of data collected (20.6 ± 8.4 entries per subject per flight week) is much greater than was collected previously (<12 per person per entire mission), despite user criticisms regarding app usability. It seems likely that improvements in a software-based questionnaire application could result in a robust data collection tool that astronauts find more acceptable, while simultaneously providing researchers and clinicians with useful data.Wotring VE, Smith LK. Dose tracker application for collecting medication use data from International Space Station crew. Aerosp Med Hum Perform. 2020; 91(1):41–45.


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