scholarly journals Iron induced genotoxicity: attenuation by vitamin C and its optimization

2014 ◽  
Vol 7 (3) ◽  
pp. 154-158 ◽  
Author(s):  
Nuzhat Parveen ◽  
Shoeb Ahmad ◽  
G.G. Hammad A. Shadab
Keyword(s):  
Dna Damage ◽  
Free Radical ◽  
Vitamin C ◽  
Free Radical Scavenger ◽  
Iron Sulfate ◽  
Radical Scavenger ◽  
Optimum Level ◽  
Acute Administration ◽  
Oxygen Species ◽  
Indirect Action

ABSTRACT Vitamin C (VC) is a well-known antioxidant and strong free radical scavenger. Its antioxidant activity is useful for protection of cellular macromolecules, particularly DNA, from oxidative damage induced by different agents. This study was undertaken to evaluate the optimum level of VC in attenuating the chromosome aberrations (CAs) and DNA damage after iron sulfate (FeSO4) acute administration in Wistar rats. The results exhibited that the increase of CAs and DNA damage induced by FeSO4, 200 mg Fe/kg, could be reduced significantly by VC pretreatment at the dose of 500 mg/kg (p<0.001), but not in the 100 mg/kg group. The findings provide evidence that VC at the dose of 500 mg/kg exerted a possible protective effect against FeSO4 induced CAs and DNA damage. The possible mechanisms of VC may be attributed to its property as a free radical scavenger or to its indirect action in reducing the level of reactive oxygen species (ROS).

scholarly journals Reactive oxygen species modulate growth of cerebral aneurysms: a study using the free radical scavenger edaravone and p47phox−/− mice

2009 ◽  
Vol 89 (7) ◽  
pp. 730-741 ◽  
Author(s):  
Tomohiro Aoki ◽  
Masaki Nishimura ◽  
Hiroharu Kataoka ◽  
Ryota Ishibashi ◽  
Kazuhiko Nozaki ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Free Radical ◽  
Cerebral Aneurysms ◽  
Reactive Oxygen ◽  
Free Radical Scavenger ◽  
Radical Scavenger ◽  
Oxygen Species

scholarly journals Bromodeoxyuridine amplifies free-radical-mediated DNA damage

1989 ◽  
Vol 261 (1) ◽  
pp. 269-271 ◽  
Author(s):  
M Poot ◽  
P S Rabinovitch ◽  
H Hoehn
Keyword(s):  
Dna Damage ◽  
Free Radical ◽  
Culture Medium ◽  
Oxygen Free Radicals ◽  
Cumene Hydroperoxide ◽  
Free Radical Scavenger ◽  
Radical Scavenger ◽  
Sensitive Cell ◽  
G2 Arrest ◽  
G2 Phase

Elevated oxygen concentrations and paraquat, a superoxide-generating compound, induce an arrest of cells in the G2 phase of the cell cycle, which can be enhanced by adding bromodeoxyuridine (BrdU) to the culture medium. Experiments with the lipophilic peroxide cumene hydroperoxide and the free-radical scavenger vitamin E demonstrate that the BrdU-dependent G2 arrest is not mediated by lipid peroxidation. The BrdU-dependency of arrest in the G2 phase can be used as a sensitive cell biological assay to detect DNA damage elicited by oxygen free radicals.

OPTIMASI KONDISI EKSTRAKSI SENYAWA AKTIF ANTIOKSIDAN DARI BIJI ANGGUR

2019 ◽  
Vol 3 (2) ◽  
pp. 35
Author(s):  
Kartini Kartini ◽  
Azminah Azminah
Keyword(s):  
Free Radical ◽  
Optimum Condition ◽  
Active Compound ◽  
Grape Seed ◽  
Free Radical Scavenger ◽  
Water Bath ◽  
Radical Scavenger ◽  
Optimum Conditions ◽  
Ethanolic Solution ◽  
Reduced Pressure

In order to prepare standardized extract, optimization of extraction conditions of grape seed has been done. These conditions are type of menstrum (50, 70 and 96% of ethanolic solution), length of extraction (1, 2 and 4 hours) also method of evaporation (reduced pressure and opened air). Activity on free radical scavenger used as parameters to determine optimum conditions. Based on EC50 (concentration which scavenge 50% amount of free radical) can be concluded that optimum condition for extracting antioxidant active compound from grape seed are 70% ethanolic solution as menstrum, length of extraction 1 hour and evaporation on opened air use water bath.

scholarly journals Possible Protective Role of Melatonin in Pediatric Infectious Diseases and Neurodevelopmental Pathologies

2020 ◽  
Vol 10 (01) ◽  
pp. e104-e109
Author(s):  
Antonio Molina-Carballo ◽  
Antonio Emilio Jerez-Calero ◽  
Antonio Muñoz-Hoyos
Keyword(s):  
Endothelial Cell ◽  
Free Radical ◽  
Chronic Administration ◽  
Protective Role ◽  
Free Radical Scavenger ◽  
Radical Scavenger ◽  
Energetic Metabolism ◽  
Beneficial Effects ◽  
Molecular Crosstalk

AbstractMelatonin, produced in every cell that possesses mitochondria, acts as an endogenous free radical scavenger, and improves energetic metabolism and immune function, by complex molecular crosstalk with other intracellular compounds. There is greatly increasing evidence regarding beneficial effects of acute and chronic administration of high melatonin doses, in infectious, developmental, and degenerative pathologies, as an endothelial cell and every cell protectant.

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