Molecular detection and genetic diversity of porcine circovirus type 3 in commercial pig farms in Xinjiang province, China

AbstractIntroductionPorcine circovirus type 3 (PCV3) is a newly discovered porcine circovirus. The molecular characteristics and genetic evolution of PCV3 in Xinjiang province, China still being unclear, the aim of the study was their elucidation.Material and MethodsA total of 393 clinical samples were collected from pigs on commercial farms in nine different regions of Xinjiang and phylogenetic analysis based on full-length Cap genes was performed.ResultsThe prevalence at farm level was 100%, while in all the tested samples it was 22.39%. Nine PCV3 strains were detected in Xinjiang province and they shared 98.9–99.3% nucleotide and 97.5–100.0% Cap gene amino acid sequence identities with other epidemic strains from China and abroad. Compared with other epidemic strains of PCV3, there were 26 base mutation sites in the Cap gene in the nine Xinjiang strains, resulting in the mutation of amino acids at positions 20, 24, 75, 77, 108, 111 and 206. Phylogenetic analysis showed that these strains can be divided into two different genetic groups, to the first of which five strains affiliated and divided between subgroups 1.1 and 1.2, and to the second of which the other four strains affiliated and similarly divided between subgroups 2.1 and 2.2.ConclusionPCV3 circulates widely among commercial pig farms in Xinjiang province, China, and displays obvious genetic diversity. The results provide epidemiological information useful for the prevention and control of PCV3 infection in the pig industry.

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Retrospective survey and phylogenetic analysis of porcine circovirus type 3 in Jiangsu province, China, 2008 to 2017

Archives of Virology ◽

10.1007/s00705-018-3870-2 ◽

2018 ◽

Vol 163 (9) ◽

pp. 2531-2538 ◽

Cited By ~ 12

Author(s):

Dong Zhao ◽

Xiaobo Wang ◽

Qingqing Gao ◽

Changchao Huan ◽

Wanbin Wang ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Porcine Circovirus Type ◽

Jiangsu Province ◽

Porcine Circovirus ◽

Retrospective Survey ◽

Type 3

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Genetic diversity and prevalence of porcine circovirus type 3 (PCV3) and type 2 (PCV2) in the Midwest of the USA during 2016–2018

Transboundary and Emerging Diseases ◽

10.1111/tbed.13467 ◽

2020 ◽

Vol 67 (3) ◽

pp. 1284-1294 ◽

Cited By ~ 6

Author(s):

Yin Wang ◽

Lance Noll ◽

Nanyan Lu ◽

Elizabeth Porter ◽

Colin Stoy ◽

...

Keyword(s):

Genetic Diversity ◽

Porcine Circovirus Type ◽

Porcine Circovirus ◽

The Usa ◽

Type 3

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Detection and phylogenetic analysis of porcine circovirus type 3 in Taiwan

Archives of Virology ◽

10.1007/s00705-020-04870-6 ◽

2020 ◽

Author(s):

Chia-Chun Chang ◽

Ching-Wei Wu ◽

Yu-Chih Chang ◽

Ching-Ying Wu ◽

Maw-Sheng Chien ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Porcine Circovirus Type ◽

Porcine Circovirus ◽

Type 3

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Complete Genome Sequence of a Novel Porcine Circovirus Type 3 Strain, CH/GX/1776D/2017, Isolated from Guangxi, China

Genome Announcements ◽

10.1128/genomea.00285-18 ◽

2018 ◽

Vol 6 (17) ◽

Cited By ~ 1

Author(s):

Bingxia Lu ◽

Yibin Qin ◽

Ying He ◽

Lei Liu ◽

Qunpeng Duan ◽

...

Keyword(s):

Genome Sequence ◽

Sequence Data ◽

Southern China ◽

The United States ◽

Porcine Circovirus Type ◽

Porcine Circovirus ◽

Molecular Characteristics ◽

Guangxi Province ◽

Swine Herds ◽

Type 3

ABSTRACT Porcine circovirus type 3 (PCV3) was first described in 2016 in U.S. swine herds as a pathogenic agent for pigs. To date, PCV3 has been reported to be widely circulating in the United States, China, South Korea, Brazil, Italy, and Poland. Here, we report the genome sequence of a PCV3 strain (CH/GX/1776D/2017) isolated from Guangxi Province in southern China. The sequence data presented in this study will help us better understand the molecular characteristics and genetic diversity of PCV3 in China.

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Molecular detection and phylogenetic analysis of porcine circovirus type 3 in 21 Provinces of China during 2015–2017

Transboundary and Emerging Diseases ◽

10.1111/tbed.13125 ◽

2019 ◽

Vol 66 (2) ◽

pp. 1004-1015 ◽

Cited By ~ 22

Author(s):

Shanshan Qi ◽

Mingjun Su ◽

Donghua Guo ◽

Chunqiu Li ◽

Shan Wei ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Molecular Detection ◽

Porcine Circovirus Type ◽

Porcine Circovirus ◽

Type 3

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Phylogenetic analysis of porcine circovirus type 2 (PCV2) between 2015 and 2018 in Henan Province, China

BMC Veterinary Research ◽

10.1186/s12917-019-2193-1 ◽

2020 ◽

Vol 16 (1) ◽

Cited By ~ 6

Author(s):

Guanmin Zheng ◽

Qingxia Lu ◽

Fangyu Wang ◽

Guangxu Xing ◽

Hua Feng ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Amino Acid ◽

Complete Genome ◽

Porcine Circovirus Type ◽

Porcine Circovirus Type 2 ◽

Henan Province ◽

Porcine Circovirus ◽

Economic Losses ◽

Clinical Samples

Abstract Background Porcine circovirus type 2 (PCV2) is the pathogen of porcine circovirus associated diseases (PCVAD) and one of the main pathogens in the global pig industry, which has brought huge economic losses to the pig industry. In recent years, there has been limited research on the prevalence of PCV2 in Henan Province. This study investigated the genotype and evolution of PCV2 in this area. Results We collected 117 clinical samples from different regions of Henan Province from 2015 to 2018. Here, we found that the PCV2 infection rate of PCV2 was 62.4%. Thirty-seven positive clinical samples were selected to amplify the complete genome of PCV2 and were sequenced. Based on the phylogenetic analysis of PCV2 ORF2 and complete genome, it was found that the 37 newly detected strains belonged to PCV2a (3 of 37), PCV2b (21 of 37) and PCV2d (13 of 37), indicating the predominant prevalence of PCV2b and PCV2d strains. In addition, we compared the amino acid sequences and found several amino acid mutation sites among different genotypes. Furthermore, the results of selective pressure analysis showed that there were 5 positive selection sites. Conclusions This study indicated the genetic diversity, molecular epidemiology and evolution of PCV2 genotypes in Henan Province during 2015–2018.

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Detection and phylogenetic analysis of porcine circovirus type 3 in central China

Transboundary and Emerging Diseases ◽

10.1111/tbed.12920 ◽

2018 ◽

Vol 65 (5) ◽

pp. 1163-1169 ◽

Cited By ~ 17

Author(s):

Peng-Li Xu ◽

Yu Zhang ◽

Yu Zhao ◽

Hui-Hua Zheng ◽

Hao-Ying Han ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Porcine Circovirus Type ◽

Central China ◽

Porcine Circovirus ◽

Type 3

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Wide Range of the Prevalence and Viral Loads of Porcine Circovirus Type 3 (PCV3) in Different Clinical Materials from 21 Polish Pig Farms

Pathogens ◽

10.3390/pathogens9050411 ◽

2020 ◽

Vol 9 (5) ◽

pp. 411

Author(s):

Aleksandra Woźniak ◽

Dagmara Miłek ◽

Tomasz Stadejek

Keyword(s):

Porcine Circovirus Type ◽

Porcine Circovirus ◽

Genome Equivalent ◽

Viral Loads ◽

Oral Fluids ◽

Wide Range ◽

Pig Farms ◽

Suckling Piglets ◽

Precise Quantification ◽

Type 3

Porcine circovirus type 3 (PCV3) was described in different clinical cases and healthy pigs. However, little is known about its circulation in pig farms. In order to assess PCV3 prevalence in 21 Polish farms, serum, feces, and oral fluid samples were examined by quantitative real-time PCR. In total, 1451 pairs of serum and feces from the same animals, as well as 327 samples of oral fluids were analyzed. The results showed that PCV3 is more commonly detected in oral fluids (37.3% positives) than in serum (9.7% positives) or feces (15.0% positives) samples. The viral loads detected in these materials ranged from 102.5–107.2 genome equivalent copies/mL. Although in most farms PCV3 was detected post weaning, in nine farms, the virus was also found in groups of suckling piglets, and in six of them viremia was detected. In four farms with reproductive failure, fetal materials were also obtained. PCV3 was detected in 36.0% of fetuses or stillborn piglets (9/25) with viral loads of 103.1–1010.4 genome equivalent copies/mL. In summary, the virus circulation may show different patterns, and congenital or early infection is not uncommon. Precise quantification of PCV3 loads in clinical materials seems to be necessary for the study and diagnosis of the infection.

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