scholarly journals Evaluation of oxidative stress parameters in dogs with brachycephalic obstructive airway syndrome before and after surgery

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Vladimira Erjavec ◽  
Tomaž Vovk ◽  
Alenka Nemec Svete
Keyword(s):  
Oxidative Stress ◽  
Clinical Signs ◽  
Respiratory Disorder ◽  
Lipid Peroxidation Product ◽  
Sod Activity ◽  
Oxidative Stress Parameters ◽  
Before And After ◽  
Status Assessment ◽  
Grade 3 ◽  
Stress Parameters

Abstract Introduction Canine brachycephalic obstructive airway syndrome (BOAS) is a conformation-related respiratory disorder of dog breeds having congenitally flattened facial and skull anatomy. The aim of the study was to determine oxidative stress parameters, the lipid peroxidation product malondialdehyde, and antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase in BOAS patients before and after surgical treatment and in healthy brachycephalic dogs. Material and Methods Nine healthy brachycephalic dogs that had not undergone surgery and 39 BOAS patients were included in the study. The BOAS patients were classified as grade 1 (5/34), grade 2 (16/34), and grade 3 (13/34) based on the decrease in the radius of the airway in the larynx. In BOAS patients, oxidative stress parameters were determined before and two weeks after surgery, while in control dogs, blood samples were collected only on inclusion to the study. Results All BOAS patients showed various degrees of improvement in clinical signs after surgery. Significantly lower (P < 0.05) SOD activity was found in grade 2 and 3 BOAS patients than in grade 1 patients. Two weeks after surgery, a significant (P < 0.05) increase in SOD activity in grade 2 and 3 patients was observed. Conclusion Antioxidant enzyme SOD may play an important role in BOAS and can be used as a biomarker of antioxidant status assessment in BOAS patients.

scholarly journals Alfacalcidol modulates oxidative stress parameters in the peripheral blood of patients with active rheumatoid arthritis

2016 ◽  
Vol 81 (10) ◽  
pp. 1127-1139 ◽  
Author(s):  
Tatjana Zivanovic-Radnic ◽  
Katarina Simic-Pasalic ◽  
Mirjana Sefik-Bukilica ◽  
Sonja Misirlic-Dencic ◽  
Andjelka Isakovic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Das28 Score ◽  
Sod Activity ◽  
Oxidative Stress Parameters ◽  
Before And After ◽  
Significant Clinical Improvement ◽  
Membrane Depolarisation ◽  
O2 Production ◽  
Gpx Activity ◽  
Stress Parameters

Hormone D and its analogues display immunomodulatory activities providing a beneficial effect in immunoinflammatory diseases. The aim of this study was to assess the effect of alfacalcidol treatment on superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activity and glutathione (GSH) and malondialdehyde (MDA) levels in patients with active RA. Sixteen patients with active RA and twenty controls were enrolled in the study. Blood samples were taken before and after 12 weeks of alfacalcidol therapy (2 ?g/day). Oxidative stress parameters were determined spectrophotometrically and by flow cytometry assessment. Disease activity was assessed using DAS28 score. The results revealed that alfacalcidol treatment, significantly (p = 0.04) reduced SOD activity and CAT activity (p = 0.001) in RA patients. The activity of GPx was significantly lower in RA patients before treatment, compared to controls (p = 0.04). After therapy, GPx activity was restored to control levels, and GSH levels were significantly reduced (p = 0.01). MDA levels in patients at the beginning of the study protocol, remained significantly elevated compared to controls (p = 0.01). Alfacalcidol treatment decreased MDA levels in patients (p = 0.19). Furthermore, 12-weeks alfacalcidol therapy, changed the response of RA patients? PBMC to stimulation preventing the O2 - production and mitochondrial membrane depolarisation. After alfacalcidol treatment, significant clinical improvement was observed.

scholarly journals Clinical Significance of ROMs, OXY, SHp and HMGB-1 in Canine Leishmaniosis

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 754
Author(s):  
Michela Pugliese ◽  
Alessandra Sfacteria ◽  
Gaetano Oliva ◽  
Annastella Falcone ◽  
Manuela Gizzarelli ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lymph Nodes ◽  
Clinical Signs ◽  
Biological Tissues ◽  
Stress Index ◽  
Control Group ◽  
Canine Leishmaniosis ◽  
Oxidative Stress Parameters ◽  
Stress Parameters

This study aimed to investigate the role of oxidative stress parameters (ROMs, OXY, SHp), the Oxidative Stress index (OSi), and High Mobility Group Box-1 protein (HMGB-1) in canine leishmaniosis (CanL). For this study, thirty dogs, naturally infected with Leishmania spp. (Leishmania Group, LEISH) and ten healthy adult dogs (control group, CTR) were included. The diagnosis of CanL was performed by a cytological examination of lymph nodes, real time polymerase chain reaction on biological tissues (lymph nodes and whole blood), and an immunofluorescence antibody test (IFAT) for the detection of anti-Leishmania antibodies associated with clinical signs such as dermatitis, lymphadenopathy, onychogryphosis, weight loss, cachexia, lameness, conjunctivitis, epistaxis, and hepatosplenomegaly. The HMGB-1 and oxidative stress parameters of the LEISH Group were compared with the values recorded in the CTR group (Mann Whitney Test, p < 0.05). Spearman rank correlation was applied to evaluate the correlation between the HMGB-1, oxidative stress biomarkers, hematological and biochemical parameters in the LEISH Group. Results showed statistically significant higher values of SHp in the LEISH Group. Specific correlation between the ROMs and the number of red blood cells, and between HGMB-1 and SHp were recorded. These preliminary data may suggest the potential role of oxidative stress in the pathogenesis of CanL. Further studies are undoubtedly required to evaluate the direct correlation between inflammation parameters with the different stages of CanL. Similarly, further research should investigate the role of ROMs in the onset of anemia.

scholarly journals Oxidative stress induced by chlorpromazine in patients treated and acutely poisoned with the drug

2016 ◽  
Vol 73 (4) ◽  
pp. 312-317 ◽  
Author(s):  
Bratislav Dejanovic ◽  
Vesna Vukovic-Dejanovic ◽  
Ivana Stevanovic ◽  
Ivana Stojanovic ◽  
Gordana Mandic-Gajic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Serum Concentration ◽  
Control Group ◽  
Sod Activity ◽  
Antioxidative Status ◽  
Oxidative Stress Parameters ◽  
Long Time ◽  
Total Antioxidative Status ◽  
Therapeutic Doses ◽  
Stress Parameters

Background/Aim. Although chlorpromazine (CPZ) is an antipsychotic drug widely used in clinical practice for a long time, its mechanism of action has not been entirely defined. An extremely difficult managing of patients acutely poisoned with CPZ is additional reason for detailed studying its toxicity mechanisms. In this clinical study, we investigated whether the oxidative stress (OS) mediates CPZ toxic effects in the exposed patients. Methods. The patients were organized into 3 groups: the T-group - hospitalized patients receiving therapeutic doses of 75-150 mg CPZ/day; the overdosed group, divided into two subgroups: the group M and the group S - mildly (CPZ serum concentration: 0.21 ? 0.05 mg/L) and severely (CPZ serum concentration: 2.66 ? 0.25 mg/L) poisoned patients, respectively, and the group C (control group of healthy volunteers). Oxidative stress parameters [total antioxidative status (TAS) and malondialdehyde (MDA) in plasma)] and superoxide dismutase (SOD) activity in erythrocytes were measured spectrophotometrically, and CPZ concentrations in serum were monitored chromatographically. One set of measurements was performed in the group C and T, whereas two sets of measurements (after 24 hours and 48 hours) were done in the poisoned patients, groups M and S. Results. A decrease of TAS and increase of SOD activity were obtained in both subgroups of the poisoned patients, compared to the controls and the group receiving therapeutic doses of CPZ. A significant increase of MDA was achieved in severely poisoned patients, compared to all other groups. Conclusion. Changed oxidative stress parameters in patients poisoned with chlorpromazine indicate involvement of oxidative stress in the toxicity mechanism(s) of chlorpromazine.

scholarly journals Effects Of Rose-Hip And Grapeseed Dietary Supplementation On Serum Oxidative Stress Parameters In Dogs Before And After Physical Exercise

2015 ◽  
Vol 65 (3) ◽  
pp. 404-416 ◽  
Author(s):  
Kukovska Valentina ◽  
Celeska Irena ◽  
Ruškovska Tatjana ◽  
Valčić Olivera
Keyword(s):  
Oxidative Stress ◽  
Physical Exercise ◽  
Statistical Significance ◽  
Dietary Supplementation ◽  
Oxidative Stress Parameters ◽  
Before And After ◽  
Antioxidative Effects ◽  
Dietary Regime ◽  
Rose Hip ◽  
Stress Parameters

AbstractThe aim of this study was to evaluate the effects of 60 days of rose-hip and grapeseed dietary supplementation of a balanced home-cooked diet on serum oxidative stress parameters: ROMs, MDA and FRAP in army service dogs before and after regular physical exercise. The dogs were fed a balanced cooked diet as instructed by army standards until the initial blood sampling in June. Thereon the dogs were randomly allotted to 4 groups according to the dietary regime: dogs maintained on a balanced cooked diet according to army standards, branded dry dog food, cooked diet with added 500 mg rose-hip extract, and cooked diet with added 100 mg grapeseed extract for a 60 day period from June to September after which all 4 groups were fed the standard cooked meal diet. Sampling was performed at the beginning of the experiment (June), 60 days from the start of the treatment (September) and finally 60 days after the end of supplementation (November). Statistical analysis of the results included descriptive statistical parameters: mean (M), standard deviation (SD), and variation coefficient (CV%). In order to test the statistical significance of the differences between treatments a multifactor variance test (ANOVA) was performed for ROM, MDA and FRAP and the combined effects of diet, exercise and time period were observed. The initial (in June) increase in MDA and ROMs after exercise indicates the presence of oxidative stress 30 minutes after exercise. However, the antioxidative effects of rose-hip and grapeseed extracts are not conclusive, as multifactor ANOVA testing of time, diet, and exercise factors did not reveal for MDA statistically significant differences either at 60 days of supplementation nor 60 days after withdrawal of the supplements. Only one distinct exception was recorded for the prolonged antioxidative effects reflected in significantly decreased (p<0.01) ROMs before and after exercise in dogs fed the branded dry food 60 days after the end of such dietary regime (November). FRAP values tend to be higher (p>0.05) after exercise compared to before exercise in all experimental groups in November, indicating on a possible redistribution and upregulation of endogenous antioxidants during the experiment.

Does human myocardium show a gender dependent modification of oxidative stress parameters?

2014 ◽  
Vol 62 (S 01) ◽  
Author(s):  
K. Buschmann ◽  
Y. Gramlich ◽  
M. Oelze ◽  
A. Daiber ◽  
T. Münzel ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Human Myocardium ◽  
Oxidative Stress Parameters ◽  
Dependent Modification ◽  
Stress Parameters

Evaluation of oxidative stress parameters in subacute thyroiditis patients

2019 ◽  
Author(s):  
Murat Dağdeviren ◽  
Arzu Or Koca ◽  
Tolga Akkan ◽  
İhsan Ateş ◽  
Salim Neşelioğlu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Subacute Thyroiditis ◽  
Oxidative Stress Parameters ◽  
Stress Parameters
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