α-HPV positivity analysis in a group of patients with melanoma and non-melanoma skin cancers / Analiza pozitivităţii α-HPV la un grup de pacienţi cu tumori cutanate melanocitare şi non-melanocitare

AbstractBackground. Medical research has shown a continuous increase in the incidence of skin cancers, especially among young individuals. One of the ethiopathogenic factors that cause skin carcinogenesis could be the infection with some genotypes of human papillomavirus (HPV). Methods. In our study, we have analyzed alpha (α) - HPV positivity and HPV genotypes identified in melanocytic (MSC) and nonmelanocytic skin cancers (NMSC). The results were then compared with results obtained from the control group. The study included 40 cases of MSC and NMSC found in the data base of our hospital, and 40 healthy patients. In all of the cases, we identified the HPV DNA by using polymerase chain reaction (PCR), and the viral genotypes by using α -HPV primers by Linear Array Roche kit. Results. The average α-HPV positivity in tumors was 32.50%, higher than in other studies published to date. The squamous cell carcinoma (SCC) lot had the highest α-HPV positivity (40%), followed by basal cell carcinoma (BCC) (35%) and malignant melanoma (MM) (20%). The comparative analysis between skin cancer-HPV positive (32.50%) and the control group-HPV positive (15%) revealed a positivity of HPV in the tumors group (32.50%) that was higher by a ratio of 2.16. By viral genotyping, we identified high-risk HPV only in BCC and MM (in all α-HPV samples), but not in SCC samples. Conclusions. In our study, α-HPV in NMSC and MSC was positive in 32.50% of the cases; in 46.15% of these, it was possible to identify HPV genotypes. The high-risk HPV genotypes observed in these patients were HPV 16, 35, 58 and 59.

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Evaluation of a New Multiplex Real-Time Polymerase Chain Reaction Assay for the Detection of Human Papillomavirus Infections in a Referral Population

International Journal of Gynecological Cancer ◽

10.1097/igc.0b013e31825529b7 ◽

2012 ◽

Vol 22 (6) ◽

pp. 1050-1056 ◽

Cited By ~ 8

Author(s):

Matthias Jentschke ◽

Philipp Soergel ◽

Victoria Lange ◽

Boštjan Kocjan ◽

Thilo Doerk ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Human Papillomavirus ◽

High Risk ◽

Polymerase Chain Reaction Assay ◽

Clinical Performance ◽

Chain Reaction ◽

Hpv Dna ◽

Hpv Genotypes ◽

Polymerase Chain ◽

High Risk Hpv

ObjectivesHuman papillomavirus (HPV) testing is an important part of cervical cancer screening and management of women with atypical screening results. This study was conducted to evaluate the analytical and clinical performance of the Abbott RealTime High-Risk HPV assay (RealTime) in a referral population, in comparison to the Qiagen Hybrid Capture 2 High-Risk HPV DNA Test (hc2).MethodsRealTime is a new polymerase chain reaction assay that detects 14 high-risk HPV genotypes with simultaneous differentiation between HPV 16 and HPV 18. Five hundred forty-five routine cervical smear samples (ThinPrep) from women who were referred to 2 German colposcopy clinics were included in the study. All samples were tested with both assays for the detection of high-risk HPV DNA. Specimens with repeatedly discordant results were genotyped by Linear Array (Roche) and in-house polymerase chain reaction assays.ResultsBoth assays showed excellent overall agreement (92.8%; κ = 0.86) on 545 samples. Analytical sensitivity of RealTime was comparable to that of hc2 (97.6% vs 95.1%,P= 0.189), whereas RealTime demonstrated significantly higher analytical specificity compared with hc2 (100% vs 93.1%,P< 0.0001). RealTime showed no cross-reactivity with untargeted HPV genotypes in this study. The clinical performance of the assays was evaluated based on histology results available from 319 women (90 nonpathological, 73 cervical intraepithelial neoplasia [CIN] 1, 75 CIN 2, 74 CIN 3, and 7 invasive cancers). High-risk HPV detection rates observed in women with CIN 1, CIN 2+, and CIN 3+ diagnosis, respectively, were comparable for both assays: 47.9%, 92.3%, and 97.5% (RealTime) and 47.9%, 92.3%, and 93.8% (hc2). Detection of HPV 16/18 with RealTime was highly correlated with severity of dysplasia: less than CIN 2, 30.5%; CIN 2+, 59.0%; CIN 3+, 71.6%.ConclusionsThese results support the use of RealTime for routine detection of HPV infections in a referral population.

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p16 expression in cutaneous squamous cell carcinoma of the head and neck is not associated with integration of high risk HPV DNA or prognosis

Pathology ◽

10.1016/j.pathol.2017.04.002 ◽

2017 ◽

Vol 49 (5) ◽

pp. 494-498 ◽

Cited By ~ 16

Author(s):

Laveniya Satgunaseelan ◽

Noel Chia ◽

Hyerim Suh ◽

Sohaib Virk ◽

Bruce Ashford ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

High Risk ◽

Cell Carcinoma ◽

Head And Neck ◽

Squamous Cell ◽

Cutaneous Squamous Cell Carcinoma ◽

Hpv Dna ◽

High Risk Hpv ◽

P16 Expression

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Treatment of Multiple Actinic Keratosis and Field of Cancerization with Topical Piroxicam 0.8% and Sunscreen 50+ in Organ Transplant Recipients: A Series of 10 Cases

Case Reports in Dermatology ◽

10.1159/000481770 ◽

2017 ◽

Vol 9 (3) ◽

pp. 211-216 ◽

Cited By ~ 13

Author(s):

Virginia Garofalo ◽

Alessandra Ventura ◽

Sara Mazzilli ◽

Laura Diluvio ◽

Luca Bianchi ◽

...

Keyword(s):

High Risk ◽

Skin Cancer ◽

Cell Carcinoma ◽

Medical Device ◽

Actinic Keratosis ◽

Organ Transplant ◽

Cyclooxygenase Inhibitors ◽

Organ Transplant Recipient ◽

Skin Cancers ◽

Melanoma Skin

Organ transplant recipient (OTR) subjects are at high risk of skin cancer such as squamous cell carcinoma and basal cell carcinoma. Actinic keratosis (AK) is considered the precursor of these non-melanoma skin cancers. Sun protection is mandatory in subjects with AK and this preventive strategy is very important in OTR. Treatment of the field of cancerization is also crucial to reduce the risk of recurrence of skin lesions in AK and non-melanoma skin cancer patients. Activation of cyclooxygenase 1 and 2 enzymes plays an important role in the pathogenesis of skin cancers. Topical application of cyclooxygenase inhibitors such as diclofenac and, more recently, piroxicam has shown to reduce AK lesions in immunocompetent subjects. A medical device containing piroxicam and SPF 50+ sunscreen filters (P+SS) has been demonstrated to be effective in reducing AK lesions and improving the field of cancerization. We report the effect of P+SS, applied for 16 weeks, in a case series of 10 OTR subjects with multiple AK lesions. P+SS treatment was associated with a relevant AK lesion reduction (>75%) in 7 patients (with a complete clearance in 3 subjects) with an improvement in the field of cancerization. This medical device could be considered a promising long-term curative and preventive treatment in OTR patients at high risk of non-melanoma skin cancers.

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Human papillomavirus genotyping using HPV DNA chip analysis in Korean women

International Journal of Gynecological Cancer ◽

10.1111/j.1525-1438.2007.00871.x ◽

2007 ◽

Vol 17 (2) ◽

pp. 497-501 ◽

Cited By ~ 19

Author(s):

H. S. Lee ◽

K. M. Kim ◽

S. M. Kim ◽

Y. D. Choi ◽

J. H. Nam ◽

...

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

Cervical Neoplasia ◽

Dna Chip ◽

Korean Women ◽

Hpv 16 ◽

Hpv Dna ◽

Hpv Genotypes ◽

High Risk Hpv ◽

Chip Analysis

This study was designed to investigate the genotypes of human papillomavirus (HPV) in Korean women who had abnormal cervical cytology and to evaluate the clinical accuracy of HPV DNA chip analysis for the diagnosis of cervical neoplasia. Liquid-based cytology preparations, HPV DNA chip analysis, and cervical biopsy were performed in 2358 women. High-risk HPV was identified in 23.5% of 1650 histologically confirmed normal samples (including cervicitis and squamous metaplasia) and in 81.8% of 708 samples with cervical intraepithelial neoplasia (CIN) and carcinoma (P< 0.01). The major prevalent high-risk HPV genotypes in 381 samples of CIN II/III were HPV-16, -58, -33, and -31, in order of prevalence rate (average overall, 78.0%), and HPV-16, -18, -58, and -33 (average overall, 81.2%) in 133 samples of squamous cell carcinoma (SCC). The infection rate of HPV-16 was significantly higher than that of other high-risk HPV genotypes in all normal, CIN, and SCC cases (P< 0.01) and increased with more advanced squamous cervical lesions (P< 0.01). The detection accuracy of high-risk HPV using HPV DNA chip analysis for CIN II or worse was as follows: sensitivity 84% (81–87%), specificity 72% (70–74%), positive predictive value 47% (44–50%), and negative predictive value 94% (92–95%). These results suggest that HPV DNA chip analysis may be a reliable diagnostic tool for the detection of cervical neoplasia and that there are geographic differences in the distribution of high-risk HPV genotypes.

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Correlation between Human Papillomavirus Codetection Profiles and Cervical Intraepithelial Neoplasia in Japanese Women

Microorganisms ◽

10.3390/microorganisms8121863 ◽

2020 ◽

Vol 8 (12) ◽

pp. 1863

Author(s):

Kaori Okayama ◽

Hirokazu Kimura ◽

Koji Teruya ◽

Yasuyoshi Ishii ◽

Kiyotaka Fujita ◽

...

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

Cervical Intraepithelial Neoplasia ◽

Intraepithelial Neoplasia ◽

Hpv Infection ◽

Hpv Genotypes ◽

High Risk Type ◽

Pcr Method ◽

Polymerase Chain ◽

High Risk Hpv

Human papillomavirus (HPV) infection is thought to be strongly associated with the precarcinomatous state cervical intraepithelial neoplasia (CIN) and cervical carcinoma. To accurately assess the correlation between HPV detection profiles and CIN, the uniplex E6/E7 polymerase chain reaction (PCR) method was used. We detected HPV (37 genotypes) in 267 CIN cases. The detection of a single high-risk HPV genotype occurred in 69.7% of CIN1 and worse than CIN1 (CIN1+) cases whereas other types were detected in 11.6% of cases. Codetection of high-risk HPV genotypes occurred in 4.9% of CIN1+ cases. The high-risk genotype HPV16 was the most frequently detected genotype in CIN1+ lesions; the genotype HPV34 (not a high-risk type) was detected in some CIN3 cases. Furthermore, HPV codetection may not be associated with CIN grades. These results suggest that various HPV genotypes are associated with CIN across all analyzed cases.

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Significance of the viral load of high-risk HPV in the diagnosis and prediction of cervical lesions: a retrospective study

BMC Women s Health ◽

10.1186/s12905-021-01493-0 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Yang Liu ◽

Changjun Xu ◽

Jing Pan ◽

Chunyi Sun ◽

Honglin Zhou ◽

...

Keyword(s):

Cervical Cancer ◽

Viral Load ◽

Retrospective Study ◽

High Risk ◽

High Viral Load ◽

Control Group ◽

Cervical Lesions ◽

Hpv Dna ◽

High Risk Hpv ◽

Hpv Viral Load

Abstract Background The significance of HPV viral load in the detection of cervical lesions is still controversial. This study analyzed the correlation between the high-risk HPV viral load and different cervical lesion degrees. Methods This retrospective study included women positive for high-risk HPV DNA and screened for cervical lesions between 01/2015 and 06/2018. The high-risk HPV DNA load was measured by the second-generation Hybrid Capture technology and classified as low, moderate, and high. Colposcopy and biopsy were performed in all patients. The patients were grouped as normal, cervical intraepithelial neoplasia (CIN) grade 1, CIN grade 2, CIN grade 3, and cervical cancer. Multivariable logistic regression was performed to explore the association between high-risk HPV DNA load and cervical lesions. The odds ratios (ORs) represent the odds for increasing from low to high viral load. Results Finally, 265 patients were grouped as normal (n = 125), CIN 1 (n = 51), CIN 2 (n = 23), CIN 3 (n = 46), and cervical cancer (n = 20). Among them, 139 (52.5%) had a low viral load, 90 (34.0) had a moderate viral load, and 36 (13.4%) had a high viral load. Taking the normal control group as a reference, a high viral load was an independent factor for CIN 1 (OR = 3.568, 95% CI: 1.164–10.941, P = 0.026), CIN 2 (OR = 6.939, 95% CI: 1.793–26.852, P = 0.005), CIN 3 (OR = 7.052, 95% CI: 2.304–21.586, P = 0.001), and cervical cancer (OR = 8.266, 95% CI: 2.120–32.233, P = 0.002). Conclusions Among women who underwent cervical biopsy, higher high-risk HPV viral load in cervical lesions was associated with a higher risk of high-grade cervical lesions.

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Assessment of Immunohistochemistry for p16INK4 and High-Risk HPV DNA by In Situ Hybridization in Esophageal Squamous Cell Carcinoma

International Journal of Surgical Pathology ◽

10.1177/1066896910382005 ◽

2011 ◽

Vol 19 (1) ◽

pp. 31-34 ◽

Cited By ~ 12

Author(s):

Sajjad M. Malik ◽

Daniel T. Nevin ◽

Sidney Cohen ◽

Jennifer L. Hunt ◽

Juan P. Palazzo

Keyword(s):

Squamous Cell Carcinoma ◽

In Situ Hybridization ◽

High Risk ◽

Esophageal Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Hpv Dna ◽

High Risk Hpv

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Cervical Dysplasia, Infection, and Phylogeny of Human Papillomavirus in HIV-Infected and HIV-Uninfected Women at a Reproductive Health Clinic in Nairobi, Kenya

BioMed Research International ◽

10.1155/2020/4945608 ◽

2020 ◽

Vol 2020 ◽

pp. 1-10

Author(s):

Agnes Omire ◽

Nancy L. M. Budambula ◽

Leah Kirumbi ◽

Hillary Langat ◽

Danvas Kerosi ◽

...

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

High Risk ◽

Reproductive Health ◽

Health Clinic ◽

Cancer Etiology ◽

Hpv 16 ◽

Hpv Dna ◽

Hpv Genotypes ◽

High Risk Hpv

High risk human Papillomavirus (HPV) infections ultimately cause cervical cancer. Human Immunodeficiency Virus (HIV) infected women often present with multiple high-risk HPV infections and are thus at a higher risk of developing cervical cancer. However, information on the circulating high-risk HPV genotypes in Kenya in both HIV-infected and HIV-uninfected women is still scanty. This study is aimed at determining the phylogeny and the HPV genotypes in women with respect to their HIV status and at correlating this with cytology results. This study was carried out among women attending the Reproductive Health Clinic at Kenyatta National Hospital, a referral hospital in Nairobi, Kenya. A cross-sectional study recruited a total of 217 women aged 18 to 50 years. Paired blood and cervical samples were obtained from consenting participants. Blood was used for serological HIV screening while cervical smears were used for cytology followed by HPV DNA extraction, HPV DNA PCR amplification, and phylogenetic analysis. Out of 217 participants, 29 (13.4%) were HIV seropositive, while 68 (31.3%) were positive for HPV DNA. Eight (3.7%) of the participants had abnormal cervical cytology. High-risk HPV 16 was the most prevalent followed by HPV 81, 73, 35, and 52. One participant had cervical cancer, was HIV infected, and had multiple high-risk infections with HPV 26, 35, and 58. HPV 16, 6, and 81 had two variants each. HPV 16 in this study clustered with HPV from Iran and Africa. This study shows the circulation of other HPV 35, 52, 73, 81, 31, 51, 45, 58, and 26 in the Kenyan population that play important roles in cancer etiology but are not included in the HPV vaccine. Data from this study could inform vaccination strategies. Additionally, this data will be useful in future epidemiological studies of HPV in Nairobi as the introduction or development of new variants can be detected.

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