Molecular identification of mycorrhizal fungi in Dactylorhiza sambucina (Orchidaceae)

AbstractWe amplified and sequenced internal transcribed spacer (ITS) region of the nuclear ribosomal repeat from fungi in roots of Dactylorhiza sambucina (Orchidaceae) and used the extended database to identify the mycorrhizal fungi. We molecularly identified three ITS recovered from D. sambucina roots, one belonging to Rhizoctonia group, and two to ascomycetes, for the first time in Orchidoidae. In many cases, two sequence types were found from the same orchid root, providing that two taxa may be involved in mycorrhizal formation (multiple mycobiont colonization). Moreover, we demonstrated that D. sambucina plants, irrespective of their colour polymorphism, possess roots containing several fungi belonging to both asco- and basidiomycetes.

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WildTermitomycesSpecies Collected from Ondo and Ekiti States Are More Related to African Species as Revealed by ITS Region of rDNA

The Scientific World JOURNAL ◽

10.1100/2012/689296 ◽

2012 ◽

Vol 2012 ◽

pp. 1-5 ◽

Cited By ~ 2

Author(s):

Victor Olusegun Oyetayo

Keyword(s):

Sequence Analysis ◽

Phylogenetic Tree ◽

Molecular Identification ◽

Internal Transcribed Spacer ◽

Its Region ◽

Its Sequence ◽

African Countries ◽

African Species ◽

Its Sequence Analysis ◽

Degree Of Similarity

Molecular identification of eighteenTermitomycesspecies collected from two states, Ondo and Ekiti in Nigeria was carried out using the internal transcribed spacer (ITS) region. The amplicons obtained from rDNA ofTermitomycesspecies were compared with existing sequences in the NCBI GenBank. The results of the ITS sequence analysis discriminated between all theTermitomycesspecies (obtained from Ondo and Ekiti States) andTermitomycessp. sequences obtained from NCBI GenBank. The degree of similarity of T1 to T18 to gene ofTermitomycessp. obtained from NCBI ranges between 82 and 99 percent.Termitomycesspecies from Garbon with ascension number AF321374 was the closest relative of T1 to T18 except T12 that has T. eurhizus and T. striatus as the closet relative. Phylogenetic tree generated with ITS sequences obtained from NCBI GenBank data revealed that T1 to T18 are more related toTermitomycesspecies indigenous to African countries such as Senegal, Congo, and Gabon.

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Three new Russula species in sect. Ingratae (Russulales, Basidiomycota) from southern China

MycoKeys ◽

10.3897/mycokeys.84.68750 ◽

2021 ◽

Vol 84 ◽

pp. 103-139

Author(s):

Guo-Jie Li ◽

Shou-Mian Li ◽

Bart Buyck ◽

Shi-Yi Zhao ◽

Xue-Jiao Xie ◽

...

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

Internal Transcribed Spacer ◽

Southern China ◽

Its Region ◽

Line Drawings ◽

Phylogenetic Data ◽

Three New Species ◽

First Time

Three new species of Russulasection Ingratae, found in Guizhou and Jiangsu Provinces, southern China, are proposed: R. straminella, R. subpectinatoides and R. succinea. Photographs, line drawings and detailed morphological descriptions for these species are provided with comparisons against closely-related taxa. Phylogenetic analysis of the internal transcribed spacer (ITS) region supported the recognition of these specimens as new species. Additionally, R. indocatillus is reported for the first time from China and morphological and phylogenetic data are provided for the Chinese specimens.

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Molecular Identification of Fusarium Spp. Isolated From Wheat Based on Sequencing of Internal Transcribed Spacer (ITS) Region

10.20944/preprints202010.0426.v1 ◽

2020 ◽

Author(s):

Deyana Gencheva ◽

Georgi Beev

Keyword(s):

Molecular Identification ◽

Internal Transcribed Spacer ◽

Its Region ◽

Genetic Distances ◽

Accurate Information ◽

Its Sequencing ◽

Fusarium Spp ◽

Internal Transcribed Spacer Region ◽

Genome Region ◽

Rdna Its

Molecular identification via Internal Transcribed Spacer region (nrDNA-ITS) sequencing of Fusarium spp. isolates from wheat originated from Stara Zagora region, were performed for the first time in Bulgaria. А total of 60 wheat samples (Triticum aestivum) were morphologically identified at the genus level as Fusarium spp. in advance. The rDNA-ITS region of all isolates was successfully amplified and the PCR products obtained were directly sequenced. After a comparison of detected sequences with NCBI database, members of three different fungal genera (Fusarium, Chaetomium, and Alternaria) were identified. Among Fusarium isolates, the F. tricinctum was prevailing, followed by F. poae. A total of three isolate F. proliferatum, F. graminearum and F. equiseti were presented with a single probe. The lowest genetic distance (0.004) was detected between F. tricinctum isolates. On the base of genetic distances, fungal isolates were grouped in two main clusters – one comprising F. tricinctum isolates and F. proliferatum, and second including F. equiseti, F. graminearum and F. poae. It could be concluded that the rDNA-ITS genome region of the genus Fusarium may be used as a suitable marker of early detection, accurate and reliable identification of Fusarium spp. contamination of wheat. The timely and accurate information would assist in the selection of appropriate approaches for control of fusarium infections and possible mycotoxins contamination of agricultural production.

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Analysis of thrips (Thysanoptera: Thripidae) diversity in avocado shows the presence of a new species and limitations of using the COI gene for differentiation of two species

Revista Colombiana de Entomología ◽

10.25100/socolen.v47i2.10754 ◽

2021 ◽

Vol 47 (2) ◽

Author(s):

Daniela Cano-Calle ◽

Clara Inés Saldamando-Benjumea ◽

Claudia Ximena Moreno-Herrera ◽

Rafael Eduardo Arango-Isaza

Keyword(s):

Internal Transcribed Spacer ◽

Pcr Amplification ◽

Its Region ◽

Morphological Characters ◽

Coi Gene ◽

Thrips Palmi ◽

Thrips Species ◽

Quarantine Species ◽

First Time ◽

A New Species

Thrips are important pests of avocados causing damage to the fruits, making themunsuitable for exportation. Many of them are quarantine species and their identification isnecessary. Despite their importance, not many studies describe thrips diversity on avocado,especially in Colombia, partly because identification of some of the species is difficult usingclassical taxonomic keys. In this study, 93 adult thrips specimens were collected on avocadoand adventitious dandelion plants from plantations in the department of Antioquia, Colombia. Morphological characters were used to classify 62 of them and all of them were analyzed by PCR amplification and sequencing of cytochrome c oxidase subunit I (COI) gene and internal transcribed spacer (ITS) region. The species identified by morphological features were Frankliniella gardeniae Moulton, F. gossypiana Hood, F. panamensis Hood, Liothrips perseae Watson, Thrips palmi Karny, T. simplex (Morison), T. trehernei Priesner, Microcephalothrips abdominalis (Crawford DL), and Scirtothrips hansoni Mound & Hoddle (Tubulifera), being F. panamensis, M. abdominalis, and S. hansoni reported for the first time on avocado in Colombia. Thrips simplex and T. trehernei were also found on adventitious dandelion plants, but not on avocado. Phylogenetic analysis of COI and ITS sequences showed that most species, except for F. gardeniae and F. gossypiana were mainly identified with the COI gene. Furthermore, FST values obtained between them suggest no genetic differentiation at COI level. The results obtained here contribute to the knowledge of the diversity of thrips species found on the avocado crop in Colombia and are meaningful for pest management.

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Mrakia panshiensis sp. nov. a new member of the Cystofilobasidiales from soil in China, and description of the teleomorphic-stage of M. arctica

MycoKeys ◽

10.3897/mycokeys.74.53433 ◽

2020 ◽

Vol 74 ◽

pp. 75-90

Author(s):

Kai-Hong Zhang ◽

Cheng-Feng Shi ◽

Chun-Yue Chai ◽

Feng-Li Hui

Keyword(s):

Internal Transcribed Spacer ◽

Northeast China ◽

Fungal Diversity ◽

Phylogenetic Analyses ◽

Its Region ◽

Morphological Characters ◽

Undescribed Species ◽

First Time ◽

Sequence Similarities ◽

Morphological Assessment

In a study on the fungal diversity in Northeast China, twelve yeast isolates were obtained from soils collected in three provinces, Helongjiang, Jilin and Liaoning. Morphological assessment and phylogenetic analyses of the nuc rDNA internal transcribed spacer (ITS) region and the D1/D2 domains of the nuc 28S rDNA (nuc 28S) gene of the 12 cultures placed them in the genus Mrakia, namely Mrakia aquatica, Mrakia arctica, Mrakia frigida, Mrakia gelida and Mrakia robertii. A total of three isolates represented a hitherto undescribed species, which is described here as M. panshiensissp. nov. (MB 834813). The species M. panshiensissp. nov. shares several morphological characters with M. niccombsii, M. aquatica, M. fibulata and M. hoshinonis. These species can be distinguished based on physiological traits and pairwise rDNA sequence similarities. The study also describes for the first time the formation of teliospores by previously described M. arctica.

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First molecular identification of wheat seed gall nematode Anguina tritici races parasitized on wheat in Iraq

Acta Universitatis Sapientiae Agriculture and Environment ◽

10.2478/ausae-2019-0001 ◽

2019 ◽

Vol 11 (1) ◽

pp. 5-15

Author(s):

Sulaiman Naif Ami ◽

Ibrahim Esa Taher ◽

Fenik Sherzad Hussen ◽

Ayoub Ibrahim Ahmed

Keyword(s):

Molecular Identification ◽

Internal Transcribed Spacer ◽

Its Region ◽

Nematode Species ◽

Agarose Gel ◽

Wheat Cultivars ◽

Wheat Seed ◽

Kurdistan Region ◽

Two Cities ◽

Template Dna

Abstract This study was conducted to identify races of wheat seed gall nematode Anguina tritici by using molecular identification for this nematode species parasitized on durum and bread wheat cultivars. Wheat seed galls of both cultivars were collected from two cities, Erbil and Duhok, from the Kurdistan Region and Iraq respectively. DNA was extracted from both nematode isolates (populations), and then PCR reactions were performed with Internal Transcribed Spacer (ITS) region using primers TW81/AB28 with 2 µl of template DNA of A. tritici for both nematode isolates on both wheat cultivars. The bands of both amplification products of PCR reactions were visualized in position about 800 bp on agarose gel, which indicates that both isolates of A. tritici belong to the same race. On the other hand, no nucleotide differences were observed between the two nematode isolations, as revealed by the sequence alignment of DNA of the internal transcribed spacer (ITS) region and phylogenetic tree, which emphasizes at the same time that both nematode isolates are of the same genetic structure or have the same identity and confirms their belonging to the same nematode race.

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First records of three Lepiota species (Agaricales, Basidiomycota) from Ukraine, with notes on a poorly known species, Lepiota subalba

Ukrainian Botanical Journal ◽

10.15407/ukrbotj78.06.373 ◽

2021 ◽

Vol 78 (6) ◽

pp. 373-380

Author(s):

Oleh Prylutskyi ◽

◽

Oleksandr Zinenko ◽

Polina Havrysh ◽

◽

...

Keyword(s):

Nucleotide Sequence ◽

Molecular Identification ◽

Ribosomal Dna ◽

New Records ◽

Its Region ◽

General Distribution ◽

Voucher Specimen ◽

Identification Methods ◽

Molecular Identification Methods ◽

First Time

New records of four species of the genus Lepiota (Agaricales, Basidiomycota) are reported from Ukraine. Three species, L. fuscovinacea, L. griseovirens, and L. roseolivida, are recorded in Ukraine for the first time, whereas a poorly known species, L. subalba, earlier known in Ukraine from a few records, is confirmed using molecular identification methods. All species reports are supplemented with original descriptions and drawings based on newly collected material, as well as data on general distribution, habitat, references to new collections and public databases. Original nucleotide sequence of the ITS region of ribosomal DNA obtained from our voucher specimen of L. subalba is provided.

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Evidence for novel and specialized mycorrhizal parasitism: the orchid Gastrodia confusa gains carbon from saprotrophic Mycena

Proceedings of The Royal Society B Biological Sciences ◽

10.1098/rspb.2008.1225 ◽

2008 ◽

Vol 276 (1657) ◽

pp. 761-767 ◽

Cited By ~ 91

Author(s):

Yuki Ogura-Tsujita ◽

Gerhard Gebauer ◽

Toshimasa Hashimoto ◽

Hidetaka Umata ◽

Tomohisa Yukawa

Keyword(s):

Molecular Identification ◽

Internal Transcribed Spacer ◽

Large Subunit ◽

Nitrogen Isotope ◽

Mass Spectrometric ◽

Free Living ◽

Saprotrophic Fungi ◽

Carbon And Nitrogen ◽

First Time ◽

Reference Samples

We investigated the physiological ecology of the Asian non-photosynthetic orchid Gastrodia confusa . We revealed its mycorrhizal partners by using molecular identification and identified its ultimate nutritional source by analysing carbon and nitrogen natural stable isotope abundances. Molecular identification using internal transcribed spacer and large subunit nrDNA sequences showed that G. confusa associates with several species of litter- and wood-decomposer Mycena fungi. The carbon and nitrogen isotope signatures of G. confusa were analysed together with photosynthetic plant reference samples and samples of the ectomycorrhizal epiparasite Monotropa uniflora . We found that G. confusa was highly enriched in 13 C but not greatly in 15 N, while M. uniflora was highly enriched in both 13 C and 15 N. The 13 C and 15 N signatures of G. confusa were the closest to those of the fruit bodies of saprotrophic fungi. Our results demonstrate for the first time using molecular and mass-spectrometric approaches that myco-heterotrophic plants gain carbon through parasitism of wood or litter decaying fungi. Furthermore, we demonstrate that, several otherwise free-living non-mycorrhizal, Mycena can be mycorrhizal partners of orchids.

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Morphological and molecular identification of four new resupinate species of Lyomyces (Hymenochaetales) from southern China

MycoKeys ◽

10.3897/mycokeys.65.48660 ◽

2020 ◽

Vol 65 ◽

pp. 101-118

Author(s):

Jun-Zhu Chen ◽

Chang-Lin Zhao

Keyword(s):

New Species ◽

Molecular Identification ◽

Internal Transcribed Spacer ◽

Southern China ◽

Phylogenetic Analyses ◽

Its Region ◽

Molecular Data ◽

Fungal Species ◽

Molecular Evidence ◽

Thin Walled

Four new wood-inhabiting fungal species, Lyomyces bambusinus, L. cremeus, L. macrosporus and L. wuliangshanensis, are proposed based on a combination of morphological and molecular evidence. Lyomyces bambusinus is characterized by resupinate basidiomata with colliculose to tuberculate hymenial surface and broadly ellipsoid, hyaline, slightly thick-walled, smooth basidiospores. Lyomyces cremeus is characterised by resupinate basidiomata with smooth, cream hymenial surface and ellipsoid, hyaline, thin-walled to slightly thick-walled basidiospores. Lyomyces macrosporus is characterized by pruinose basidiomata with reticulate hymenial surface, presence of three kinds of cystidia and larger basidiospores (6.7–8.9 × 4.4–5.4 µm). Lyomyces wuliangshanensis is characterized by coriaceous basidiomata and ellipsoid, hyaline, slightly thick-walled, smooth basidiospores. The phylogenetic analyses based on molecular data of the internal transcribed spacer (ITS) region sequences revealed that the four new species belonged to Lyomyces. Lyomyces bambusinus grouped with L. sambuci. Lyomyces cremeus clade was sister to a clade comprised of L. microfasciculatus. Lyomyces macrosporus was sister to L. allantosporus. Lyomyces wuliangshanensis was closely related to L. mascarensis.

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Molecular phylogeny of Hiptage (Malpighiaceae) reveals a new species from Southwest China

PhytoKeys ◽

10.3897/phytokeys.135.37011 ◽

2019 ◽

Vol 135 ◽

pp. 91-104 ◽

Cited By ~ 1

Author(s):

Ke Tan ◽

Hai-Lei Zheng ◽

Shu-Peng Dong ◽

Ming-Xun Ren

Keyword(s):

New Species ◽

Molecular Phylogeny ◽

Internal Transcribed Spacer ◽

Southwest China ◽

Common Ancestor ◽

Its Region ◽

Recent Common Ancestor ◽

Most Recent Common Ancestor ◽

First Time ◽

A New Species

Hiptage is an Asia-endemic genus of Malpighiaceae currently placed in the tetrapteroid clade, representing one of the seven inter-continent dispersions from New to Old World. A molecular phylogeny based on sequences of the internal transcribed spacer (ITS) region was recovered for the first time for the genus. Our results showed that the most recent common ancestor of Hiptage probably originated in the South Indo-China Peninsula and diversified in this region. Based on phylogenetic evidence and relevant morphological traits, we propose a new species; Hiptage incurvatum is characterised by mericarps with arcuate anterior lateral wings, two large glands on the dorsal sepals, and small glands on the remaining sepals. The new species is from Mt. Cangshan, Dali City (25°35'N, 100°02'E) in North Yunnan, Southwest China and is notable for its occurrence at high altitude, 1400 m (the highest distribution currently known for the genus). The implications of this unusual species for the dispersal and evolution of the genus are discussed.

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