Chemical Properties of Zwitterionic Imidazolium Alkanecarboxylates Studied in Gas-Phase by Electrospray Ionization – Collision-Induced Dissociation / Cviterjonu Tipa Imidazolija Alkānkarboksilātu Ķīmiskās Īpašības Gāzes Fāzē, Kas Pētītas Ar Elektroizsmidzināšanas Jonizāciju – Sadursmju Inducēto Disociāciju

2012 ◽  
Vol 51 (3) ◽  
pp. 249-256
Author(s):  
A. Podjava ◽  
P. Mekss ◽  
A. Zicmanis ◽  
S. Krasnov
Keyword(s):  
Gas Phase ◽  
Chemical Properties ◽  
Collision Induced Dissociation ◽  
Potassium Ions ◽  
Hydrogen Deuterium Exchange ◽  
Carboxylate Group ◽  
Hydrogen Deuterium ◽  
Ionization Mode ◽  
Sodium And Potassium ◽  
Phase Chemical

Gas-phase chemical properties of several (1-methylimidazol-3-io)-alkane-1-carboxylates (alkane=ethane, propane and butane) have been investigated in this study. These substances are synthesized using classical transformations and analyzed in positive ionization mode using collision-induced dissociation (0-50 eV). These experiments were carried out in both deuterated and undeuterated solvent media. The data obtained in this study show, that carboxylate group weakly influences fragmentation of zwitterionic imidazolium carboxylates in positive electrospray mode. On the other hand, these compounds exert a tendency to form various adducts with sodium and potassium ions and to participate in hydrogen/deuterium exchange in the gas phase.

Hydrogen-deuterium exchange reactions of carbanions with deuterated alcohols in the gas phase

1978 ◽  
Vol 100 (9) ◽  
pp. 2921-2922 ◽  
Author(s):  
C. H. DePuy ◽  
Veronica M. Bierbaum ◽  
Gary K. King ◽  
R. H. Shapiro
Keyword(s):  
Gas Phase ◽  
Deuterium Exchange ◽  
Hydrogen Deuterium Exchange ◽  
Exchange Reactions ◽  
Hydrogen Deuterium

Do electrospray mass spectra reflect the ligand binding state of proteins in solution?

2005 ◽  
Vol 83 (11) ◽  
pp. 1953-1960 ◽  
Author(s):  
Belal M Hossain ◽  
Douglas A Simmons ◽  
Lars Konermann
Keyword(s):  
Mass Spectrometry ◽  
Gas Phase ◽  
Protein Interactions ◽  
Noncovalent Complex ◽  
Deuterium Exchange ◽  
Solution Phase ◽  
Hydrogen Deuterium Exchange ◽  
Esi Ms ◽  
Protein Ions ◽  
Hydrogen Deuterium

Electrospray ionization (ESI) mass spectrometry (MS) has become a popular tool for monitoring ligand–protein and protein–protein interactions. Due to the "gentle" nature of the ionization process, it is often possible to transfer weakly bound complexes into the gas phase, thus making them amenable to MS detection. One problem with this technique is the potential occurrence of fragmentation events during ESI. Also, some analytes tend to cluster together during ionization, thus forming nonspecific gas-phase assemblies that do not represent solution-phase complexes. In this work, we implemented a hydrogen–deuterium exchange (HDX) approach that can reveal whether or not the free and (or) bound constituents of a complex observed in ESI-MS reflect the binding situation in solution. Proteins are subjected to ESI immediately following an isotopic labeling pulse; only ligand-free and ligand-bound protein ions that were formed directly from the corresponding solution-phase species showed different HDX levels. Using myoglobin as a model system, it is demonstrated that this approach can readily distinguish scenarios where the heme–protein interactions were disrupted in solution from those where dissociation of the complex occurred in the gas phase. Experiments on cytochrome c strongly suggest that dimeric protein ions observed in ESI-MS reflect aggregates that were formed in solution.Key words: electrospray mass spectrometry, ligand–protein interaction, noncovalent complex, hydrogen–deuterium exchange, protein folding.

scholarly journals The periodic table – an experimenter’s guide to transactinide chemistry

2019 ◽  
Vol 107 (9-11) ◽  
pp. 865-877 ◽  
Author(s):  
Robert Eichler
Keyword(s):  
Research And Development ◽  
Gas Phase ◽  
Periodic Table ◽  
Chemical Properties ◽  
Superheavy Elements ◽  
Experimental Results ◽  
Chemical Studies ◽  
Phase Chemical

Abstract The fundamental principles of the periodic table guide the research and development of the challenging experiments with transactinide elements. This guidance is elucidated together with experimental results from gas phase chemical studies of the transactinide elements with the atomic numbers 104–108 and 112–114. Some deduced chemical properties of these superheavy elements are presented here in conjunction with trends established by the periodic table. Finally, prospects are presented for further chemical investigations of transactinides based on trends in the periodic table.

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