scholarly journals New Basal Media for Protocorm-Like Body and Callus Induction of Hybrid Cymbidium

2012 ◽  
Vol 20 (2) ◽  
pp. 127-133 ◽  
Author(s):  
Jaime A. Teixeira da Silva
Keyword(s):  
Callus Induction ◽  
High Frequency ◽  
Callus Formation ◽  
Basal Medium ◽  
Naphthaleneacetic Acid ◽  
Control Medium ◽  
Industry Standard ◽  
Basal Media ◽  
Bacto Agar

Abstract High frequency protocorm-like body (PLB) production from hybrid Cymbidium Twilight Moon ‘Day Light’ has been developed through a new medium, Teixeira Cymbidium (TC) medium. Two new TC media containing variable amounts of macroand micronutrients and other additives, inspired by Winarto and Teixeira (WT) medium for Anthurium and Murashige and Skoog (MS) basal medium were used to induce PLBs and callus. Control medium was research- and industry-standard Vacin and Went (VW) medium. The first TC medium, TCPLB, could induce significantly more PLBs than on VW while high levels of macronutrients in the second TC medium, TCCALLUS, and MS were required to induce callus. All PLB induction media contained 0.1 mg/l α-naphthaleneacetic acid (NAA) and 0.1 mg/l kinetin (KIN), 2 g/l tryptone and 20 g/l sucrose, and solidified with 8 g/l Bacto agar while callusinduction media were identical, except that KIN was substituted by thidiazuron (TDZ). Basal medium had a significant effect on PLB and callus formation. This protocol could be used to induce PLBs and callus from other Cymbidium species or cultivars.

scholarly journals Bioproduction of Diosgenin in Callus Cultures of Balanites aegyptiaca: Effect of Growth Regulators, Explants and Somatic Embryogenesis

2006 ◽  
Vol 1 (3) ◽  
pp. 1934578X0600100
Author(s):  
Bishnu P. Chapagain ◽  
Vinod Saharan ◽  
Dan Pelah ◽  
Ram C. Yadav ◽  
Zeev Wiesman
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Embryogenic Callus ◽  
Somatic Embryos ◽  
Callus Formation ◽  
Basal Medium ◽  
Callus Cultures ◽  
Suspension Cultures ◽  
Balanites Aegyptiaca ◽  
Basal Media

This study describes the effects of plant growth regulators, explants, and somatic embryogenesis on in vitro production of the steroidal sapogenin, diosgenin, in callus cultures of the Balanites aegyptiaca (L.) Del.(desert date). Root, shoot, hypocotyl, and epicotyl callus culture of B. aegyptiaca, were raised on MS basal media supplemented with various combinations of either 2,4-D and NAA alone, or with BAP. The diosgenin content (on a dry weight basis) was found to be highest when calli were cultured in MS basal medium supplemented with 1.0 mg l−1 2,4-D alone and/or in combination with 0.5 mg l−1 BAP. However, the callus growth was highest in media supplemented with 2.5 or 3.0 mg l−1 2,4-D. MS basal media supplemented with 2,4-D 2.5 mg l−1 alone and in combination with 0.5 mg l−1 BAP induced pre-embryogenic callus formation on root cultures. When these pre-embryogenic callus cultures were used to establish cell suspension cultures, two growth densities were obtained in embryogenic suspension cultures, inducing clusters of somatic embryos at various stages of development. The maximum number of somatic embryos were obtained at the fifth week on the medium supplemented with 1.0 mg l−1 2,4-D. However, the diosgenin content in these somatic cells was found to be lower compared to the explant calluses. This study revealed that production of diosgenin in callus cultures of B. aegyptiaca is possible, but the amount is significantly affected by the growth regulators, type of explants, and somatic embryogenesis.

scholarly journals 252 STUDIES ON ADVENTITIOUS REGENERATION IN COCOYAM

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 465f-465
Author(s):  
Leopold M. Nyochembeng ◽  
Stephen Garton
Keyword(s):  
High Frequency ◽  
Somatic Embryos ◽  
Callus Formation ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Adventitious Shoot ◽  
Shoot Tips ◽  
Adventitious Regeneration ◽  
Petiole Explants ◽  
The Impact

Studies were conducted to determine the response of cocoyam shoot tips, petioles, cotyledons and hypocotyls in various media for callus formation, adventitious shoot development and somatic embryogenesis. In all experiments, B5 basal medium or low N B5 were supplemented with various growth regulators. High frequency adventitious shoot proliferation was obtained using cotyledons and hypocotyls in medium supplemented with 1 mg/l IBA and 0.5 mg/l TDZ. Embryogenic callus was obtained using shoot tips in media containing 1 mg/l Dicamba, hile somatic embryos were observed in media containing 0.3 mg/l 2, 4 - D and 1 mg/l Kinetin, using hypocotyl and petiole explants. The impact of these results on micropropagation of cocoyam is discussed.

Ovule and Embryo Culture of Arachis hypogaea and Interspecific Hybrids1

1988 ◽  
Vol 15 (2) ◽  
pp. 98-104 ◽  
Author(s):  
H. T. Stalker ◽  
M. A. Eweda
Keyword(s):  
Embryo Culture ◽  
Interspecific Hybrids ◽  
Shoot Growth ◽  
Basal Medium ◽  
Lower Percentage ◽  
Naphthaleneacetic Acid ◽  
Wide Crosses ◽  
In Vitro Techniques ◽  
Basal Media

Abstract Interspecific hybridization in Arachis is difficult between species within sectional groups and nearly impossible among more distantly related species. Embryos usually abort early in the reproductive cycle; thus in vitro techniques are necessary to recover many desirable hybrid combinations in the genus. The objectives of this investigation were to develop techniques whereby mature plants could be recovered from otherwise aborting embryos. First, ovule culture was performed using eight genotypes, three levels of kinetin, and the two basal media Murashige and Skoog (MS) and N6. Two-tenths mg/L kinetin in media resulted in 24% of the ovules swelling to a size of 3-4 mm which could be used for excising embryos. Embryo culture was next performed on five genotypes. The transfer series (I) 0.2 mg/L kinetin for 21 days, (2) 0.5 mg/L 6-benzylamino-purine (BAP) for 14 days and, (3) MS without growth regulators resulted in 34.6% of ovules producing plants across genotypes; other transfer series either resulted in a lower percentage of plant recovery and/or tissues of some genotypes which did not survive to maturity. The BAP medium induced shoot growth, while root growth was induced on the MS without growth regulator medium. Approximately 90% of embryos transferred to a mist system after 7-9 weeks in vitro survived transplanting to soil. Two interspecific hybrids were recovered from incompatible hexaploid x diploid crosses, but only after roots were induced using a MS basal medium with 4 mg/L 1-naphthaleneacetic acid:2 mg/L indole-3-butyric acid in a fourth tissue transfer. The experiments illustrated the feasibility of rescuing embryos of A. hypogaea and interspecific peanut hybrids. The process is slow and will be most applicable to wide crosses which cannot be obtained by more conventional methods.

scholarly journals In vitro regeneration of bulblet using two and four bulb-scales explants of summer snowflake (Leucojum aestivum L.)

2021 ◽  
Vol 27 (2) ◽  
pp. 221-231
Author(s):  
Masoumeh Abedinimazraeh ◽  
Sepideh Kalatehjari
Keyword(s):  
Callus Induction ◽  
Large Scale ◽  
In Vitro Regeneration ◽  
Hot Water ◽  
Naphthaleneacetic Acid ◽  
Scale Production ◽  
Control Medium ◽  
Indirect Organogenesis ◽  
Natural Form ◽  
Leucojum Aestivum

Abstract Leucojum aestivum is a valuable and endangered plant species with bulb scales best suited as explants in micropropagation. In the current study, its micropropagation was investigated by using two different explants and various concentrations and combinations of plant growth regulators (PGRs). Bulbs were first disinfected with benomyl® for 5 hours. After meeting the chilling requirements, two-scale and four-scale explants were provided for direct and indirect organogenesis. Explants were exposed to hot water, 70% ethanol and 2.5% sodium hypochlorite for further disinfestation. Four-scale explants were treated with different concentrations and combinations of naphthaleneacetic acid (NAA), 6-benzyladenine (BA), and kinetin (Kin) for bulblet regeneration. For callogenesis, 0.5 mg L-1 of BA combined with 1, 2, 3, 4, 5 or 6 mg L-1 of 2,4-dichlorophenoxyacetic acid (2,4-D) were applied. Regarding two-scale explants, different combinations and concentrations of BA, Indole-3-butyric acid (IBA) and NAA were used for bulblet induction, and various combinations of Indoleacetic acid (IAA), NAA, 2, 4-D and BA were used for callus induction. None of the two-scale explants responded to the bulblet regeneration and callus induction media. Unlike, four-scale explants regenerated bulblets and roots in the control medium and MS media enriched with different PGRs. Callus was generated on MS medium supplemented with 2,4-D and BA, and indirect regeneration was observed in some cases. On the control medium, the regenerated roots had a natural form, but in PGRs-rich media, they were deformed. Regarding the regeneration percentage, bulblet number and length and root length, no significant differences were found between the control and the best PGR-treatment in each case. Therefore, it seems logical suggesting not to use PGRs, which will considerably reduce the costs at large-scale production.

scholarly journals Micropropagation and Field Evaluation of × Heucherella ‘Bridget Bloom’

1990 ◽  
Vol 8 (3) ◽  
pp. 156-159
Author(s):  
S.L. Kitto ◽  
J.J. Frett ◽  
P. Geiselhart
Keyword(s):  
Adventitious Shoots ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Field Evaluation ◽  
Axillary Shoot ◽  
Naphthaleneacetic Acid ◽  
Axillary Shoot Proliferation ◽  
Murashige Skoog ◽  
Micropropagated Plants ◽  
Bacto Agar

Abstract × Heucherella ‘Bridget Bloom’ shoots were surface disinfected and cultured on basal medium composed of Murashige-Skoog salts and vitamins and the following addenda per liter; sucrose, 30 g; glycine, 2 mg; and washed Difco Bacto-agar, 6 g. Axillary shoot proliferation was greatest on medium supplemented with 0.5 mg 1−1 benzyladenine, 0.025 mg 1−1 naphthaleneacetic acid and 4 g 1−1 washed Difco Bacto-agar. Adventitious shoots regenerated from callus that initiated from the base of cultured microcuttings. Microcuttings were rooted in Redi-Earth under intermittent mist for 4 wk (94% rooted) and then moved to a greenhouse (98% survival after 4 wk). During a 16 month field study, plants produced from microcuttings grew as well as, if not better than, greenhouse-grown plants propagated by division. Micropropagated plants originating from axillary buds had significantly greater fresh and dry weights, and initiated more leaves and crowns when grown under field conditions than plants originating from adventitious buds.

scholarly journals Fast protocol for high frequency in vitro cloning of Banana (Musa acuminata) cv. Grande Naine

2017 ◽  
Vol 9 (1) ◽  
pp. 72-79
Author(s):  
S. R. Parida ◽  
S. Beura ◽  
S. Rout ◽  
R. Beura ◽  
P. N. Jagadev
Keyword(s):  
High Frequency ◽  
Basal Medium ◽  
Shoot Multiplication ◽  
Musa Acuminata ◽  
Future Research ◽  
Naphthaleneacetic Acid ◽  
Maximum Height ◽  
Ms Medium ◽  
Number Of Leaves

An investigation was conducted on Fast Protocol for High Frequency in vitro cloning of Banana (Musa acuminata) cv. Grande Naine at the Biotechnology-cum-Tissue Culture Center, OUAT, Bhubaneswar, during the year 2012. This has helped to determine the best media compositions for shoot multiplication and rooting of cv. Grande Naine, so as to get optimum results with a minimized cost of production. MS medium supplemented with 4.0 mg/1 Benzylaminopurine (BAP) and 2.0 mg/1 Kinetin gave the highest number of shoot/explants (11.33) in 30 days. However, MS medium when supplemented with 6.0 mg/1 BAP produced a maximum number of leaves (19.07) with a maximum height 2.73 cm. Among various concentrations of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and naphthaleneacetic acid (NAA) for rooting. Half MS medium supplemented with 1.0 mg/1 IBA was found to be ideal for early rooting and producing more number of roots in 21 days. However, MS basal medium was found to be the best treatment to support the formation of long roots. This protocol can be very useful to the future research worker and as well as entrepreneurs for mass production of banana (Musa acuminata) cv. Grande Naine.

scholarly journals Somatic embryogenesis from leaf transverse thin cell layer derived-callus of Vietnamese ginseng (Panax vietnamensis Ha et Grushv.)

2016 ◽  
Vol 14 (1) ◽  
pp. 63-73
Author(s):  
Vu Thi Hien ◽  
Nguyen Phuc Huy ◽  
Bui Van The Vinh ◽  
Hoang Xuan Chien ◽  
Hoang Thanh Tung ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Cell Layer ◽  
Culture Media ◽  
Callus Formation ◽  
Basal Medium ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Transverse Thin Cell Layer ◽  
Cell Layers ◽  
2,4 Dichlorophenoxyacetic Acid

No report on plant regeneration via somatic embryogenesis of P. vietnamensis has been previously published. In the present study, somatic embryogenesis via callus formation from cultures of leaf transverse thin cell layers (tTCLs) of Vietnamese ginseng (Panax vietnamensis Ha et Grushv.) was investigated. α-naphthaleneacetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BA) and thidiazuron (TDZ) were added separately and in combination into the culture media. Explant necrosis or low callogenesis rates were observed when 1-mm wide leaf tTCLs were cultured on media with TDZ, BA, 2,4-D or NAA. On the other hand, calli were successfully induced from the tTCL explants cultured on medium supplemented with either 2,4-D and BA or 2,4-D and TDZ. Callogenesis was observed under both light and dark conditions. The highest callogenesis rate (100%) was obtained on Murashige and Skoog (MS) basal medium supplemented with 1.0 mg l-1 2,4-D in combination with 0.1 mg l-1 TDZ in darkness after eight weeks of culture. White calli were cut into small pieces (1.0 x 1.0 cm dimension) and placed on MS media containing 1.0 mg l-1 2,4-D, 0.5 mg l-1 NAA and TDZ at various concentrations (0.01; 0.1; 0.2; and 0.5 mg l-1), and the best callus proliferation was recorded on medium containing 1.0 mg l-1 2,4-D and 0.2 mg l-1 TDZ. Somatic embryogenesis, with a success rate of 53.3% and 35 embryos per explant, was achieved when calli were subcultured onto MS medium supplemented with 1.0 mg l-1 2,4-D, 0.5 mg l-1 NAA and 0.2 mg l-1 TDZ.

scholarly journals CALLUS FORMATION AND PLANT REGENERATION FROM IMMATURE EMBRYOS OF PIGMENTED UPLAND RICE (Oryza sativa cv. Tadong)

2021 ◽  
Vol 83 (4) ◽  
pp. 91-100
Author(s):  
Hoo Kah Yan ◽  
Lee Ping Chin ◽  
Mariam A. Latip ◽  
Noumie Surugau ◽  
Zaleha A. Aziz
Keyword(s):  
Shoot Regeneration ◽  
Callus Induction ◽  
Upland Rice ◽  
Callus Formation ◽  
Rice Cultivar ◽  
Immature Embryos ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Improvement Programs ◽  
Rice Improvement

  The application of biotechnology in upland rice improvement programs depends on the availability of efficient regeneration protocols.  Although protocols for shoot regeneration of upland rice are available, none has been reported for pigmented cultivars.  This study reports on a protocol for callus induction and regeneration of Tadong, a pigmented upland rice cultivar from Sabah.  For callus induction, immature embryos were cultured on media containing 2,4-Dichlorophenoxyacetic (2,4-D) at various concentrations (0 – 2.5 mg/L) and on different types of media (MS; MSB5; N6B5; N6).  To induce shoot regeneration, callus explants were cultured on MS medium supplemented with combinations of 6-Benzylaminopurine (BAP) at various concentrations (0 – 3.0 mg/L) and 1-Naphthaleneacetic acid (NAA) at 1.0 mg/L.  To induce shoot development, callus explants were pre-treated with Thidiazuron (TDZ) at various concentrations (0-1.0 mg/l) and exposed to different desiccation periods (0 – 72 hours).  2,4-Dichlorophenoxyacetic at 2.5 mg/L and N6B5 medium resulted in the highest percentages of explant forming callus which were 60.3 ± 17.0 % and 58.7 ± 9.8 % respectively.   The regeneration media failed to induce shoot on callus explants, instead, green spots were formed on the surface of the callus.  The green spots were stimulated to develop into shoots when the callus explants were pre-treated with 0.5 mg/L TDZ or exposed to partial desiccation for 24 h, the percentages of explant forming shoot were 35.7 ± 4.8 % and 47.7 ± 6.8 % respectively.   Shoots developed into complete plants on hormone-free MS medium and acclimatized.

High-Frequency Shoot Regeneration From Flower Bud Derived Callus of Gymnostachyum Febrifugum Benth., an Endemic Medicinal Plant to the Western Ghats

2021 ◽  
Author(s):  
Silpa P ◽  
Dennis Thuruthiyil Thomas
Keyword(s):  
Callus Induction ◽  
High Frequency ◽  
Shoot Organogenesis ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Flower Bud ◽  
The Family ◽  
Endemic Medicinal Plant ◽  
Dichlorophenoxy Acetic Acid ◽  
The Western Ghats

Abstract Gymnostachyum febrifugum Benth. is a small, scapigerous, rare and endemic medicinal herb indigenous to India belonging to the family Acanthaceae. This study reports an efficient protocol for high-frequency flower bud derived callus induction and shoot organogenesis in G. febrifugum. Flower buds at 7d before anthesis (dBA) were excised from the inflorescence and cultured on MS medium supplemented with various concentrations of 2, 4-dichlorophenoxy acetic acid (2, 4-D; 0.5-2.0 mg/l) for callus induction. The optimum callus induction (78%) was obtained on MS medium supplemented with 1.5 mg/l 2, 4-D. The calli when subcultured on MS medium supplemented with different concentrations of thidiazuron (TDZ; 0.5-2.5 mg/l) or 6-benzylaminopurine BAP (0.5-2.5 mg/l) alone or in combination with 1- naphthaleneacetic acid (NAA; 0.2-0.7 mg/l) induced shoots. The highest frequency (94%) and number of shoots (44.6 shoots/unit callus) were obtained on MS medium supplemented with 2.0 mg/l TDZ and 0.5 mg/l NAA. The optimum rooting frequency (95%) and number of roots (10.2) were observed on ½ MS medium supplemented with 3.0 mg/l indole-3- butyric acid (IBA). The rooted plantlets were acclimatized and transferred to soil with 94% success.

Callus formation and shoot bud differentiation in anther culture of Solanum surattense

1979 ◽  
Vol 57 (22) ◽  
pp. 2524-2527 ◽  
Author(s):  
S. Sinha ◽  
R. P. Roy ◽  
K. K. Jha
Keyword(s):  
Anther Culture ◽  
Callus Formation ◽  
Low Frequency ◽  
Basal Medium ◽  
Pollen Grains ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Cytological Examination ◽  
Shoot Bud ◽  
2,4 Dichlorophenoxyacetic Acid

In anther culture of Solatium surattense, the Murashige and Skoog's medium supplemented with 2,4-dichlorophenoxyacetic acid (2.2 mg/L), indoleacetic acid or naphthaleneacetic acid (1.9 mg/L), and kinetin (2.2 mg/L) served as “callus-producing medium.” Histological and cytological observations indicated that the callus originated from the pollen grains. Synergistic action of kinetin (5.0 mg/L) and coconut milk (15%) in basal medium was able to induce differentiation of shoot buds either from the anthers directly or from the callus. Directly differentiating buds were formed by whole shoot bud morphogenesis of pollen. They were produced at a low frequency and showed presence of well-developed radicular and plumular regions. But the buds originating from callus lacked radicular ends. Root initiation in such buds was achieved by transferring them to basal medium. Cytological examination of the androgenic plantlets revealed a chromosomal series ranging from the haploid to the hexaploid with a few aneuploids.

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