Isolation and characterization of the genes encoding antimicrobial neutrophil cationic peptides, defensins.

Ensho ◽  
1995 ◽  
Vol 15 (1) ◽  
pp. 33-41
Author(s):  
Isao Nagaoka ◽  
Noriko Ishihara ◽  
Akimasa Someya ◽  
Kazuhisa Iwabuchi ◽  
Shin Yomogida ◽  
...  
Keyword(s):  
Cationic Peptides ◽  
Isolation And Characterization ◽  
Genes Encoding

Analysis of the structural genes encoding M-factor in the fission yeast Schizosaccharomyces pombe: identification of a third gene, mfm3

1994 ◽  
Vol 14 (6) ◽  
pp. 3895-3905
Author(s):  
S Kjaerulff ◽  
J Davey ◽  
O Nielsen
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Mutational Analysis ◽  
M Cells ◽  
Gene Products ◽  
Structural Genes ◽  
Triple Mutant ◽  
Isolation And Characterization ◽  
Genes Encoding

We previously identified two genes, mfm1 and mfm2, with the potential to encode the M-factor mating pheromone of the fission yeast Schizosaccharomyces pombe (J. Davey, EMBO J. 11:951-960, 1992), but further analysis revealed that a mutant strain lacking both genes still produced active M-factor. Here we describe the isolation and characterization of a third M-factor gene, mfm3. A mutant lacking all three genes fails to produce M-factor, indicating that all functional M-factor genes now have been identified. The triple mutant exhibits an absolute mating defect in M cells, a defect that is not rescued by addition of exogenous M-factor. A mutational analysis reveals that all three mfm genes contribute to the production of M-factor. Their transcription is limited to M cells and requires the mat1-Mc and ste11 gene products. Each gene is induced when the cells are starved of nitrogen and further induced by a pheromone signal. Additionally, the signal transduction machinery associated with the pheromone response is required for transcription of the mfm genes in both stimulated and unstimulated cells.

scholarly journals Isolation and Characterization of the Genes Encoding Basic and Acidic Chitinase in Arabidopsis thaliana

1990 ◽  
Vol 93 (3) ◽  
pp. 907-914 ◽  
Author(s):  
Deborah A. Samac ◽  
Cathy M. Hironaka ◽  
Peter E. Yallaly ◽  
Dilip M. Shah
Keyword(s):  
Arabidopsis Thaliana ◽  
Isolation And Characterization ◽  
Genes Encoding

Isolation and characterization of genes encoding lipid transfer proteins in Linum usitatissimum

2016 ◽  
Vol 60 (2) ◽  
pp. 285-291
Author(s):  
V. A. Mhaske ◽  
R. Datla ◽  
S. Qiu ◽  
A. M. Harsulkar
Keyword(s):  
Linum Usitatissimum ◽  
Lipid Transfer ◽  
Lipid Transfer Proteins ◽  
Isolation And Characterization ◽  
Genes Encoding

Isolation and characterization of two DREB1 genes encoding dehydration-responsive element binding proteins in chicory (Cichorium intybus)

2013 ◽  
Vol 73 (1) ◽  
pp. 45-55 ◽  
Author(s):  
Mingxiang Liang ◽  
Dandan Chen ◽  
Manman Lin ◽  
Qingsong Zheng ◽  
Zengrong Huang ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Cichorium Intybus ◽  
Isolation And Characterization ◽  
Genes Encoding ◽  
Responsive Element

Isolation and characterization of 15 genes encoding ribosomal proteins in wheat (Triticum aestivum L.)

Plant Science ◽  
2006 ◽  
Vol 170 (3) ◽  
pp. 579-586 ◽  
Author(s):  
Yingyin Yao ◽  
Zhongfu Ni ◽  
Jinkun Du ◽  
Xiuli Wang ◽  
Haiyan Wu ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Ribosomal Proteins ◽  
Triticum Aestivum L ◽  
Isolation And Characterization ◽  
Genes Encoding

scholarly journals Isolation and characterization of cDNA clones for neutrophil cationic peptides.

Ensho ◽  
1992 ◽  
Vol 12 (3) ◽  
pp. 227-232
Author(s):  
Isao Nagaoka ◽  
Shin Yomogida ◽  
Akimasa Someya ◽  
Kazuhisa Iwabuchi ◽  
Tatsuhisa Yamashita
Keyword(s):  
Cdna Clones ◽  
Cationic Peptides ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of Bacillus endophytic strains producers for non ribosomal lipopeptides NRLPs from tomato

2018 ◽  
Vol 9 (1) ◽  
pp. 128
Author(s):  
Walaa Hussein ◽  
Ramadan WA ◽  
Sameh Fahim
Keyword(s):  
High Performance ◽  
Plant Defence ◽  
Plant Diseases ◽  
Bacillus Species ◽  
Vegetable Crops ◽  
Defence Mechanisms ◽  
Isolation And Characterization ◽  
Genes Encoding ◽  
Plant Defence Mechanisms

Tomato (Solanum lycopersicum) are consid­ered one of the most important vegetable crops and infected by numbers of different diseases. Studying the use of biological alternatives, instead of chemical substances against plant diseases became necessary for the treatment by beneficial microorganisms endophytes, which can excrete natural products benefits to plant in reducing disease severity, promoting growth and inducing plant defence mechanisms. In this work, three endophytes strains were isolated from tomato stems and their 16srDNA have been found to belong to Bacillus species. The first strain was named BMG100, the second BMG101 and the third BMG102. Two Bacillus strains BMG100 and BMG101 have been found to harbour synthetases genes from three lipopeptides families; surfactin, plipastatin, and iturin (mycosubtilin) which can be detected by degenerated primers designed to detect the presence of synthetases genes encoding lipopeptides. The lipopeptides production was proved by their quantification using High Performance Liquid Chromatography (HPLC), whereas BMG100 produced 105, 178 and 293 mg/L of plipastatin, mycosubtilin and surfactin, respectively, BMG101 produced 385 mg/L of surfactin and 236 mg/L of mycosubtilin, while BMG102 showed no lipopeptides production. Keywords: Tomato; Endophytic bacteria; Lipopeptides; Bacillus species

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