scholarly journals Improvement of Sperm Motility of Rainbow Trout (Oncorhynchus mykiss W., 1792) by Supplementation of L-Arginine

2019 ◽  
Vol 7 (9) ◽  
pp. 1300
Author(s):  
Durali Danabaş ◽  
Mehmet Kocabaş ◽  
Filiz Kutluyer
Keyword(s):  
Nitric Oxide ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Sperm Motility ◽  
Motile Sperm ◽  
Promoting Effect ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Amino Acid Precursor ◽  
Arginine Concentration ◽  
Acid Precursor

In this study, the effect of supplementing amino acid-precursor of nitric oxide (NO) L-arginine were examined on sperm motility of rainbow trout Oncorhynchus mykiss. Different concentrations [0 mM (Control), 1 mM, 2 mM, 4 mM and 8 mM] of L-arginine were used in the study. To assess the effects on percentage of motile sperm and longevity, L-arginine was added to activation medium containing NaCl (52 mM). The higher L-arginine concentration (4mM) has promoting-effect on sperm motility. The treatments containing L-arginine caused significant effect on percentage of motile sperm and longevity. Overall, the findings of the present study indicated that supplementation of L-arginine may have improving effects on sperm motility of rainbow trout.

Cryopreservation of rainbow trout Oncorhynchus mykiss spermatozoa: Effects of extender supplemented with different antioxidants on sperm motility, velocity and fertility

Cryobiology ◽  
2014 ◽  
Vol 69 (3) ◽  
pp. 462-466 ◽  
Author(s):  
Filiz Kutluyer ◽  
Murathan Kayim ◽  
Fatih Öğretmen ◽  
Serhat Büyükleblebici ◽  
P. Barbaros Tuncer
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Sperm Motility ◽  
Rainbow Trout Oncorhynchus Mykiss

Rainbow trout (Oncorhynchus mykiss) possess two somatostatin mRNAs that are differentially expressed

1999 ◽  
Vol 277 (6) ◽  
pp. R1553-R1561 ◽  
Author(s):  
Craig A. Moore ◽  
Jeffrey D. Kittilson ◽  
Melissa M. Ehrman ◽  
Mark A. Sheridan
Keyword(s):  
Rainbow Trout ◽  
Amino Acid ◽  
Endocrine Pancreas ◽  
Amino Acid Identity ◽  
Differentially Expressed ◽  
Mrna Levels ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Amino Acid Precursor ◽  
Lower Intestine ◽  
Acid Precursor

Previously, we isolated a 624-bp cDNA encoding for a 115-amino acid preprosomatostatin containing [Tyr7,Gly10]-somatostatin (SS)-14 (now designated PPSS-II′) obtained from the endocrine pancreas (Brockmann bodies) of rainbow trout. In this study we have characterized a second cDNA obtained from trout pancreas that is 600-bp in length and encodes for a 111-amino acid precursor containing [Tyr7,Gly10]-SS-14 (PPSS-II′′). The nucleotide and amino acid identity between the two cDNAs is 82.3 and 80.5%, respectively. Both PPSS-II′ and PPSS-II′′ mRNA were present in esophagus, pyloric ceca, stomach, upper and lower intestine, and pancreas, whereas only SS-II′′ mRNA was present in brain. PPSS-II′′ mRNA was more abundant than PPSS-II′ mRNA in pancreas, whereas PPSS-II′ mRNA was more abundant than PPSS-II′′ mRNA in stomach. Fasting increased pancreatic PPSS-II′′ mRNA levels but had no effect on the levels of PPSS-II′ mRNA. These results indicate the existence of two nonallelic pancreatic SS-II genes that are differentially expressed, both in terms of distribution among tissues and in terms of relative abundance within the tissues.

146 Effect of pH on Rainbow trout (Oncorhynchus mykiss) Sperm Motility Using Five Extender Solutions

2018 ◽  
Vol 30 (1) ◽  
pp. 213
Author(s):  
M. Á. Peralta-Martínez ◽  
S. R. García ◽  
M. E. Kjelland ◽  
H. González-Márquez
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Sperm Motility ◽  
Salt Solution ◽  
Pond Water ◽  
Effect Of Ph ◽  
Sperm Activation ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Abdominal Massage ◽  
Sexually Mature

Rainbow trout (Oncorhynchus mykiss) sperm extender protocols can differ considerably with regards to composition of the extenders and handling. The objective of this study was to determine the effect of pH for 5 extenders on rainbow trout sperm motility and activation during storage at 5°C. Two-year-old sexually mature rainbow trout males (n = 46) weighing 1.5 to 2.5 kg were caught at Aquaculture Farm Tatakay in Jilotzingo, Estado de México, México. One semen sample per male was collected by abdominal massage. For the experiments, 100 mL of each extender (306, 512, Mounibs, Erdahl and Graham, and Hanks’ Balanced Salt Solution) was made, with pH adjusted to 6 levels (7.0, 7.2, 7.4, 7.8, 8.0, and 8.2). To evaluate pH dynamics in each extender, pH was tested daily over an 8-day storage interval. Trout sperm was added to each of the extenders in a ratio of 1:1 and motility recorded. Afterwards, each activator solution [DIA 532, saline solution (0.85%) and pond water] was added separately to a sample of the extended sperm, to initiate sperm activation. Motility was evaluated subjectively at 400× and monitored until ~99% of the sperm stopped moving (on average, in 40 s). A one-way ANOVA was used and statistical differences were set at α < 0.05. Extender 512 activated sperm motility when pH was >7.4, whereas extender 306 activated motility at every pH. Hanks’ Balanced Salt Solution activated motility at very low percentages for various pH levels, whereas Mounibs and Erdahal and Graham solutions did not activate motility for any pH tested. The 512 extender with a pH of 7 performed best (P < 0.05) as a storage solution, producing a sperm motility of 54% after activation using DIA 532. These results demonstrated the importance of evaluating effects of sperm extender pH over both short- and medium-term storage.

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