scholarly journals Recurrent Pregnancy Loss in Two Patients with High Density of NK Cells CD56+ and CD16+ in Ovulatory Cervical Mucus and in Endometrium (Case Report)

2019 ◽  
Vol 5 (1) ◽  
pp. 1-5
Author(s):  
Zdenka Ulcova-Gallova ◽  
Keyword(s):  
Case Report ◽  
Nk Cells ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
High Density ◽  
Cervical Mucus

scholarly journals Sildenafil Citrate Downregulates PDE5A mRNA Expression in Women with Recurrent Pregnancy Loss without Altering Angiogenic Factors—A Preliminary Study

2021 ◽  
Vol 10 (21) ◽  
pp. 5086
Author(s):  
Monika Kniotek ◽  
Aleksander Roszczyk ◽  
Michał Zych ◽  
Małgorzata Wrzosek ◽  
Monika Szafarowska ◽  
...  
Keyword(s):  
Nk Cells ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Nk Cell ◽  
Cell Activity ◽  
No Synthase ◽  
Angiogenic Factors ◽  
Nk Cell Activity ◽  
Culture Supernatants ◽  
Fertile Women

In our previous study, we showed that sildenafil citrate (SC), a selective PDE5A blocker, modulated NK cell activity in patients with recurrent pregnancy loss, which correlated with positive pregnancy outcomes. It was found that NK cells had a pivotal role in decidualization, angiogenesis, spiral artery remodeling, and the regulation of trophoblast invasion. Thus, in the current study, we determined the effects of SC on angiogenic factor expression and production, as well as idNK cell activity in the presence of nitric synthase blocker L-NMMA. Methods: NK cells (CD56+) were isolated from the peripheral blood of 15 patients and 15 fertile women on MACS columns and cultured in transformation media containing IL-15, TGF-β, and AZA—a methylation agent—for 7 days in hypoxia (94% N2, 1% O2, 5% CO2). Cultures were set up in four variants: (1) with SC, (2) without SC, (3) with NO, a synthase blocker, and (4) with SC and NO synthase blocker. NK cell activity was determined after 7 days of culturing as CD107a expression after an additional 4h of stimulation with K562 erythroleukemia cells. The expression of the PDE5A, VEGF-A, PIGF, IL-8, and RENBP genes was determined with quantitative real-time PCR (qRT-PCR) using TaqMan probes and ELISA was used to measure the concentrations of VEGF-A, PLGF, IL-8, Ang-I, Ang-II, IFN–γ proteins in culture supernatants after SC supplementation. Results: SC downregulated PDE5A expression and had no effect on other studied angiogenic factors. VEGF-A expression was increased in RPL patients compared with fertile women. Similarly, VEGF production was enhanced in RPL patients’ supernatants and SC increased the concentration of PIGF in culture supernatants. SC did not affect the expression or concentration of other studied factors, nor idNK cell activity, regardless of NO synthase blockade.

The Risk of Recurrent Pregnancy Loss in Patients with a High Body Mass Index: An Evidence-Based Case Report

2020 ◽  
Vol 19 (10) ◽  
pp. 460-467
Author(s):  
Tofan Widya Utam ◽  
Lowilius Wiyono ◽  
Nathalia Isabella M ◽  
Nadine Herdwita Putri Soer ◽  
Karel Handito Syafi Suma ◽  
...  
Keyword(s):  
Body Mass Index ◽  
Case Report ◽  
Body Mass ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
High Body Mass Index ◽  
Evidence Based ◽  
High Body

scholarly journals Misplaced intrauterine contraceptive devices: a case report

2021 ◽  
Vol 10 (12) ◽  
pp. 4596
Author(s):  
Nidhi D. Thakkar ◽  
Jaydeep D. Hindocha
Keyword(s):  
Case Report ◽  
Clinical Examination ◽  
Contraceptive Method ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Clinical Symptoms ◽  
Contraceptive Devices ◽  
Intrauterine Contraceptive ◽  
Intrauterine Contraceptive Devices

Contraception is a new topic gaining attention in country like India and a new proposed area of research. Intrauterine contraceptive devices (IUCDs) are one contraceptive method requiring less efforts and having more efficacy. ‘Misplaced IUCD’ is one of the rare and the most dreadful complication of IUCD insertion. Clinically, it can present as having pain, bleeding, recurrent pregnancy loss or can be asymptomatic. This is a case report of a 40 year old P4A1L4 female who presented to us with suspicion of misplaced IUCD without clinical symptoms. Clinical examination and radiological investigations made a diagnosis of misplaced IUCD and laparoscopy confirmed it. Laparoscopically misplaced IUCD was removed. 

scholarly journals Characterization of Uterine NK Cells in Women with Infertility or Recurrent Pregnancy Loss and Associated Endometriosis

2014 ◽  
Vol 72 (3) ◽  
pp. 262-269 ◽  
Author(s):  
Emma Giuliani ◽  
Kirstin L. Parkin ◽  
Bruce A. Lessey ◽  
Steven L. Young ◽  
Asgerally T. Fazleabas
Keyword(s):  
Nk Cells ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Uterine Nk Cells

scholarly journals Systemic Characterization of Novel Immune Cell Phenotypes in Recurrent Pregnancy Loss

2021 ◽  
Vol 12 ◽  
Author(s):  
Hong Liu ◽  
Xin-Xiu Lin ◽  
Xiao-Bo Huang ◽  
Dong-Hui Huang ◽  
Su Song ◽  
...  
Keyword(s):  
T Cells ◽  
Nk Cells ◽  
Peripheral Blood ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Immune Cell ◽  
Γδ T Cells ◽  
Immune Cell Subsets ◽  
History Of ◽  
Cell Phenotypes

Recurrent pregnancy loss (RPL) is a disturbing disease in women, and 50% of RPL is reported to be associated with immune dysfunction. Most previous studies of RPL focused mainly on the relationship between RPL and either T cells or natural killer (NK) cells in peripheral blood and the decidua; few studies presented the systemic profiles of the peripheral immune cell subsets in RPL women. Herein, we simultaneously detected 63 immune cell phenotypes in the peripheral blood from nonpregnant women (NPW), women with a history of normal pregnancy (NP) and women with a history of RPL (RPL) by multi-parameter flow cytometry. The results demonstrated that the percentages of naïve CD4+ T cells, central memory CD4+ T cells, naïve CD8+ T cells, mature NK cells, Vδ1+ T cells and the ratio of Vδ1+ T cells/Vδ2+ T cells were significantly higher in the RPL group than those in the NPW and NP groups, whereas the percentages of terminal differentiated CD4+ T cells, effective memory CD4+ T cells, immature NK cells and Vδ2+ T cells were significantly lower in the RPL group than those in the NPW and NP groups. Interestingly, we found that peripheral T helper (TPH) cells were more abundant in the NPW group than in the NP and RPL groups. Moreover, the percentage of Vδ2+PD-1+ gamma-delta (γδ) T cells was extremely high, above the 95th percentile limit, in the NP group compared with the NPW and RPL groups, which has never been reported before. In addition, we also determined the 5th percentile lower limit and 95th percentile upper limit of the significantly changed immunological parameters based on the files of the NPW group. Taken together, this is the first study to simultaneously characterize the multiple immune cell subsets in the peripheral blood at a relatively large scale in RPL, which might provide a global readout of the immune status for clinicians to identify clinically-relevant immune disorders and guide them to make clear and individualized advice and treatment plans.

Case Report Hepatic Infarction in a Pregnant Patient with the ‘Primary’ Antiphospholipid Syndrome

Lupus ◽  
1993 ◽  
Vol 2 (1_suppl) ◽  
pp. 275-279 ◽  
Author(s):  
A. Millán-Mon ◽  
J.L. Porto ◽  
C. Novo ◽  
C. García-Martin ◽  
D. Guitián
Keyword(s):  
Differential Diagnosis ◽  
Case Report ◽  
Antiphospholipid Syndrome ◽  
Oral Contraceptives ◽  
Lupus Anticoagulant ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Pregnant Patient ◽  
Primary Antiphospholipid Syndrome ◽  
Hepatic Infarction

We describe a patient with previous venous thrombosis while using oral contraceptives and recurrent pregnancy loss, who presented with massive hepatic infarction in the last trimester of the fourth gestation. Thrombocytopenia, the lupus anticoagulant (LA) and the anti-cardiolipin antibody (aCL) were detected and a diagnosis of a ‘primary’ antiphospholipid syndrome (APS) was made. The clinical and histological manifestations and the differential diagnosis, especially with DIC and pre-eclampsia, are discussed.

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