Identifikasi Dermatofita Pada Sisir Tukang Pangkas Di Kelurahan Jati Kota Padang

Hifzil Husni; Ennesta Asri; Rina Gustia

doi:10.25077/jka.v7i3.882

Identifikasi Dermatofita Pada Sisir Tukang Pangkas Di Kelurahan Jati Kota Padang

Jurnal Kesehatan Andalas ◽

10.25077/jka.v7.i3.p331-335.2018 ◽

2018 ◽

Vol 7 (3) ◽

pp. 331

Author(s):

Hifzil Husni ◽

Ennesta Asri ◽

Rina Gustia

Keyword(s):

Trichophyton Mentagrophytes ◽

Trichophyton Schoenleinii

Tinea kapitis merupakan kelainan kulit pada daerah kepala berambut yang disebabkan oleh jamur dermatofita. Jamur dermatofita dapat ditularkan secara langsung dari manusia, hewan, tanah dan secara tidak langsung melalui benda seperti handuk, topi dan sisir yang digunakan bergantian. Alat pangkas dapat menjadi sumber penularan infeksi secara tidak langsung. Tujuan penelitian ini adalah mengidentifikasi dermatofita pada sisir tukang pangkas di kelurahan Jati Padang. Jenis penelitian ini adalah deskriptif observasional. Populasi dalam penelitian ini adalah seluruh sisir yang digunakan tukang pangkas di Kelurahan Jati Kecamatan Padang Timur Kota Padang. Teknik pengambilan sampel pada penelitian ini adalah total sampling sebanyak 20 sampel. Penelitian ini dilaksanakan dari Agustus 2017 sampai Maret 2018. Hasil penelitian ditemukan dua spesies dermatofita yaitu Trichophyton Mentagrophytes (10%) dan Trichophyton Schoenleinii (5%). Simpulan penelitian ini adalah sebagian kecil sisir tukang pangkas di Kelurahan Jati terdapat dermatofita, oleh karena itu sisir tukang pangkas dapat menjadi media transmisi sumber infeksi yang baik pada masyarakat.

Download Full-text

Phylogenetic Classification and Species Identification of Dermatophyte Strains Based on DNA Sequences of Nuclear Ribosomal Internal Transcribed Spacer 1 Regions

Journal of Clinical Microbiology ◽

10.1128/jcm.37.4.920-924.1999 ◽

1999 ◽

Vol 37 (4) ◽

pp. 920-924 ◽

Cited By ~ 158

Author(s):

Koichi Makimura ◽

Yoshiko Tamura ◽

Takashi Mochizuki ◽

Atsuhiko Hasegawa ◽

Yoshito Tajiri ◽

...

Keyword(s):

Dna Sequences ◽

Internal Transcribed Spacer ◽

Trichophyton Mentagrophytes ◽

Bootstrap Support ◽

Its1 Region ◽

Monophyletic Cluster ◽

Arthroderma Benhamiae ◽

Base Sequences ◽

Trichophyton Schoenleinii ◽

Trichophyton Terrestre

The mutual phylogenetic relationships of dermatophytes of the genera Trichophyton, Microsporum, andEpidermophyton were demonstrated by using internal transcribed spacer 1 (ITS1) region ribosomal DNA sequences.Trichophyton spp. and Microsporum spp. form a cluster in the phylogenetic tree with Epidermophyton floccosum as an outgroup, and within this cluster, allTrichophyton spp. except Trichophyton terrestreform a nested cluster (100% bootstrap support). Members of dermatophytes in the cluster of Trichophyton spp. were classified into three groups with ITS1 homologies, with each of them being a monophyletic cluster (100% bootstrap support). TheArthroderma vanbreuseghemii-Arthroderma simii group consists of A. vanbreuseghemii, A. simii,Trichophyton mentagrophytes isolates from humans, T. mentagrophytes var. quinckeanum, Trichophyton tonsurans, and Trichophyton schoenleinii. Arthroderma benhamiae, T. mentagrophytes var.erinacei, and Trichophyton verrucosum are members of the Arthroderma benhamiae group.Trichophyton rubrum and Trichophyton violaceumform the T. rubrum group. This suggests that these “species” of dermatophytes have been overclassified. The ITS1 sequences of 11 clinical isolates were also determined to identify the species, and all strains were successfully identified by comparison of their base sequences with those in the ITS1 DNA sequence database.

Download Full-text

Modified Sabouraud dextrose agar for isolation and identification of dermatophytes

Zbornik Matice srpske za prirodne nauke ◽

10.2298/zmspn0508291b ◽

2005 ◽

pp. 291-299

Author(s):

Nina Bulajic ◽

Dragana Ivanovic ◽

Milan Savic

Keyword(s):

Trichophyton Mentagrophytes ◽

Microsporum Canis ◽

Isolation And Identification ◽

Sabouraud Agar ◽

Sabouraud Dextrose Agar ◽

Media Type ◽

Trichophyton Soudanense ◽

Causative Agents ◽

The Media ◽

Trichophyton Schoenleinii

The most common causative agents of dermatomycoses are fungi belonging to genders Trichophyton, Microsporum and Epidermophyton. Media mainly used for isolation of dermatophytes are mycobiotic agar, dermatophyte test medium Sabouraud agar (original formula or modification by Emmons) with or without antibiotics and cycloheximide. Peptones are the most important components of the media, which enable adequate reproductivity in identification of dermatophytes. Standard medium for isolation of dermatophytes is not produced in our country. The aim of the study was to create an optimal easily accessible and economic medium which enables isolation and identification of dermatophytes according to criteria for morphological diagnosis provided by identification guides. We examined 57 strains of Trichophyton, 24 of Microsporum and 5 of Epidermophyton floccosum (E. floccosum). Each strain was seeded on Sabouraud dextrose agar (Torlak Serbia and Montenegro), Sabouraud maltose agar (Torlak), two experimental modified Sabouraud dextrose agar media marked as SA-2 and SA-3 (Torlak) Sabouraud-Chloramp- henicole agar (Biomerieux, France) Sabouraud-Chloramphenicole agar (Himedia, India), Glucose-peptone agar (Himedia, India) and Sabouraud Emmons dextrose Agar with Chloramphenicole and Cycloheximide (Biolife, Italy). Colony morphology of Trichophyton mentagrophytes (T. mentagrophytes) was uni- form on all the media, while morphology of Trichophyton rubrum (T. rubrum) and Microsporum canis (M. canis) depended more on the media type. Colonies of E. floccosum were typical and uniform on all the media, as were the control species of Trichophyton schoenleinii (T. schoenleinii) and Trichophyton soudanense (T. soudanense). Experimental modified Sabouraud dextrose agar (Torlak) marked as SA-3 demonstrated the best results in identification of dermatophytes in this study.

Download Full-text

Tinea Corporis Caused by Trichophyton mentagrophytes from a Pet Rabbit

Nishi Nihon Hifuka ◽

10.2336/nishinihonhifu.66.34 ◽

2004 ◽

Vol 66 (1) ◽

pp. 34-36 ◽

Cited By ~ 2

Author(s):

Yukie YAMAGUCHI ◽

Tetsuo SASAKI ◽

Rui KANO

Keyword(s):

Trichophyton Mentagrophytes ◽

Tinea Corporis

Download Full-text

Faculty Opinions recommendation of Accidental Trichophyton mentagrophytes fungemia during the course of kerion celsi.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.13189956.14531054 ◽

2011 ◽

Author(s):

Roberto Arenas

Keyword(s):

Trichophyton Mentagrophytes ◽

Kerion Celsi

Download Full-text

Steroid Metabolism as a Mechanism of Escape from Progesterone-mediated Growth Inhibition in Trichophyton mentagrophytes

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)60447-8 ◽

1989 ◽

Vol 264 (19) ◽

pp. 11186-11192

Author(s):

K V Clemons ◽

E P Stover ◽

G Schär ◽

P A Stathis ◽

K Chan ◽

...

Keyword(s):

Growth Inhibition ◽

Trichophyton Mentagrophytes ◽

Steroid Metabolism

Download Full-text

Mechanism of streptomyces albidoflavus STV1572a derived 1-heneicosanol as an inhibitor against squalene epoxidase of Trichophyton mentagrophytes

Microbial Pathogenesis ◽

10.1016/j.micpath.2021.104853 ◽

2021 ◽

pp. 104853

Author(s):

Aswani Thekkangil ◽

Benu George ◽

S.M. Udaya Prakash ◽

T.V. Suchithra

Keyword(s):

Trichophyton Mentagrophytes ◽

Squalene Epoxidase ◽

Streptomyces Albidoflavus

Download Full-text

Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency

Antibiotics ◽

10.3390/antibiotics10060737 ◽

2021 ◽

Vol 10 (6) ◽

pp. 737

Author(s):

Marina Pekmezovic ◽

Melina Kalagasidis Krusic ◽

Ivana Malagurski ◽

Jelena Milovanovic ◽

Karolina Stępień ◽

...

Keyword(s):

Fungal Growth ◽

Pathogenic Fungi ◽

Trichophyton Mentagrophytes ◽

Microsporum Gypseum ◽

Medium Chain Length ◽

Gold Drugs ◽

Growth Inhibitory Activity ◽

Transdermal Application ◽

Antifungal Efficiency

Novel biodegradable and biocompatible formulations of “old” but “gold” drugs such as nystatin (Nys) and amphotericin B (AmB) were made using a biopolymer as a matrix. Medium chain length polyhydroxyalkanoates (mcl-PHA) were used to formulate both polyenes (Nys and AmB) in the form of films (~50 µm). Thermal properties and stability of the materials were not significantly altered by the incorporation of polyenes in mcl-PHA, but polyene containing materials were more hydrophobic. These formulations were tested in vitro against a panel of pathogenic fungi and for antibiofilm properties. The films containing 0.1 to 2 weight % polyenes showed good activity and sustained polyene release for up to 4 days. A PHA monomer, namely 3-hydroxydecanoic acid (C10-OH), was added to the films to achieve an enhanced synergistic effect with polyenes against fungal growth. Mcl-PHA based polyene formulations showed excellent growth inhibitory activity against both Candida yeasts (C. albicans ATCC 1023, C. albicans SC5314 (ATCC MYA-2876), C. parapsilosis ATCC 22019) and filamentous fungi (Aspergillus fumigatus ATCC 13073; Trichophyton mentagrophytes ATCC 9533, Microsporum gypseum ATCC 24102). All antifungal PHA film preparations prevented the formation of a C. albicans biofilm, while they were not efficient in eradication of mature biofilms, rendering them suitable for the transdermal application or as coatings of implants.

Download Full-text

Comparative clinical evaluation of efficacy and safety of a formulation containing ciclopirox 8% in the form of a therapeutic nail lacquer in two different posologies for the treatment of onychomycosis of the toes

Anais Brasileiros de Dermatologia ◽

10.1590/s0365-05962012000100002 ◽

2012 ◽

Vol 87 (1) ◽

pp. 19-25 ◽

Cited By ~ 6

Author(s):

Sergio Schalka ◽

Samanta Nunes ◽

Antonio Gomes Neto

Keyword(s):

Treatment Resistance ◽

Comparative Trial ◽

Trichophyton Mentagrophytes ◽

Weekly Dose ◽

Efficacy And Safety ◽

Therapeutic Success ◽

Group I ◽

Nail Lacquer ◽

Significant Difference ◽

Treatment Objective

BACKGROUND: The use of topical antifungal agents in the treatment of onychomycosis is of great value in clinical practice as there are different limitations regarding the use of systemic treatment. OBJECTIVE: To evaluate the efficacy and safety of a nail lacquer formulation containing ciclopirox 8% in two different posologies: the traditional regimen (3/2/1) and a regimen of weekly use. METHODS: A blind, randomized, comparative trial which included 41 patients divided into 02 groups, with Group I using the nail lacquer once weekly and Group II using the traditional regimen (3/2/1). Both groups applied the medication for 06 months. RESULTS: The species most frequently found in groups I and II were Trichophyton rubrum (55% and 61.9%) and Trichophyton mentagrophytes (30% and 19%). There was a tendency to a higher level of treatment resistance by T. mentagrophytes infection in both groups, without any predilection for sex, age, proportion of the nail affected at the beginning of the study, duration of the clinical disease and quantity of nails affected per person. Both groups had significant levels of mycological cure, clinical response and therapeutic success and there was no statistically significant difference between groups I and II (p >0.05). CONCLUSION: The nail lacquer containing ciclopirox 8% was equally effective at a weekly dose when compared to the traditional dosing (3/2/1), allowing a more comfortable regimen.

Download Full-text