scholarly journals Response of morphological and biochemical traits and transcriptomic of CDPKs and P5CS Genes to phytohormones and nutrients in two bread wheat germplasms under salinity conditions

2021 ◽  
Vol 26 (6) ◽  
pp. 3054-3061
Author(s):  
ELHADI HADIA ◽  
AMOR SLAMA ◽  
AZIZA ZOGHLAMI ◽  
LEILA ROMDHANE ◽  
AHMED HOUSSEIN ABODOMA ◽  
...  
Keyword(s):  
Salt Stress ◽  
Bread Wheat ◽  
Molecular Level ◽  
Wheat Varieties ◽  
Calcium Dependent ◽  
Genes Expression ◽  
Giberellic Acid ◽  
Dependent Protein ◽  
Biochemical Level ◽  
Calcium Dependent Protein Kinases

Several enzymes play an important role in the biosynthesis of osmolyte in plants. The main objective of this work is to study the effects of salt stress, kinetin+potassium and giberellic acid+potassium on calcium-dependent protein kinases (CDPKs) and delta-1-pyrroline-5- carboxylate synthase (P5CS) genes expression of two bread wheat varieties. The results shodwe that, salts stress reduced the plant growth of the two wheat germplasms. Moreover, the addition of kinetin+ potassium improved the performance of morphometric parameters while the addition of giberellic acid +potassium has less effect. On the biochemical level, results indicated that salt stress increased the proline contents compared with control plants. Extra increase in proline contents was recorded by kinetin+ potassium, while the treatment of giberellic acid +potassium showed almost similar results as in salt stress only. On the molecular level, salt stress, kinetin and giberellic acid significantly increased the two genes expression of CDPKs and P5CS with more effect in presence of kinetin+ potassium.

Characterization of Arabidopsis calcium-dependent protein kinases: activated or not by calcium?

2012 ◽  
Vol 447 (2) ◽  
pp. 291-299 ◽  
Author(s):  
Marie Boudsocq ◽  
Marie-Jo Droillard ◽  
Leslie Regad ◽  
Christiane Laurière
Keyword(s):  
Protein Kinases ◽  
Calcium Binding ◽  
Molecular Mechanisms ◽  
Calcium Sensitivity ◽  
In Planta ◽  
Calcium Dependent ◽  
Dependent Protein ◽  
Biochemical Level ◽  
Calcium Dependent Protein Kinases ◽  
Ef Hands

CDPKs (calcium-dependent protein kinases), which contain both calmodulin-like calcium binding and serine/threonine protein kinase domains, are only present in plants and some protozoans. Upon activation by a stimulus, they transduce the signal through phosphorylation cascades to induce downstream responses, including transcriptional regulation. To understand the functional specificities of CDPKs, 14 Arabidopsis CPKs (CDPKs in plants) representative of the three main subgroups were characterized at the biochemical level, using HA (haemagglutinin)-tagged CPKs expressed in planta. Most of them were partially or mainly associated with membranes, in agreement with acylation predictions. Importantly, CPKs displayed highly variable calcium-dependences for their kinase activities: seven CPKs from subgroups 1 and 2 were clearly sensitive to calcium with different intensities, whereas six CPKs from subgroup 3 exhibited low or no calcium sensitivity to two generic substrates. Interestingly, this apparent calcium-independence correlated with significant alterations in the predicted EF-hands of these kinases, although they all bound calcium. The noticeable exception, CPK25, was calcium-independent owing to the absence of functional EF-hands. Taken together, the results of the present study suggest that calcium binding differentially affects CDPK isoforms that may be activated by distinct molecular mechanisms.

Isolation and functional characterisation of CDPKs gene from Arachis hypogaea under salt stress

2015 ◽  
Vol 42 (3) ◽  
pp. 274 ◽  
Author(s):  
Yan Li ◽  
Feng Fang ◽  
Feng Guo ◽  
Jing-Jing Meng ◽  
Xin-Guo Li ◽  
...  
Keyword(s):  
Amino Acids ◽  
Salt Stress ◽  
Coding Region ◽  
Antioxidant Defence ◽  
Calcium Dependent ◽  
Functional Characterisation ◽  
Antioxidant Defence System ◽  
Glycine Max L ◽  
Dependent Protein ◽  
Calcium Dependent Protein Kinases

One of salt-induced calcium-dependent protein kinases (CDPKs) gene was isolated from Arachis hypogeae L. by RACE method. The cDNA full length was 2241bp deposited in GenBank (number KF437909), designated as AhCDPK. The coding region sequence of AhCDPK was 1629bp and encoded a protein of 542 amino acids. The molecular weight and the theoretical isoelectric point of AhCDPK was 60.96kDa and 5.61 respectively. Amino acid sequence analysis indicated that AhCDPK has highest similarity and homology with Glycine max L. In addition, the AhCDPK amino acids were predicted to encode a hydrophilic protein which localised in the endoplasmic reticulum. AhCDPK seemed to transcript in all peanut organs, and had the highest expression in seeds. The expression of AhCDPK could be strongly induced by both Ca2+ and NaCl. When exposed to salt stress, overexpressing AhCDPK in tobacco could alleviate PSII photoinhibition by improving physiological states, such as reducing the accumulation of reactive oxygen species (ROS), improving the activity of antioxidant defence system enzymes and improving the accumulation of osmotic regulation substance. These results showed that AhCDPK has the same functions as that of G. max, and it could play an important role for peanut to resist salt stress.

scholarly journals Salt Stress Triggers Phosphorylation of the Arabidopsis Vacuolar K+ Channel TPK1 by Calcium-Dependent Protein Kinases (CDPKs)

2013 ◽  
Vol 6 (4) ◽  
pp. 1274-1289 ◽  
Author(s):  
Andreas Latz ◽  
Norbert Mehlmer ◽  
Simone Zapf ◽  
Thomas D. Mueller ◽  
Bernhard Wurzinger ◽  
...  
Keyword(s):  
Salt Stress ◽  
Protein Kinases ◽  
K Channel ◽  
Calcium Dependent ◽  
Dependent Protein ◽  
Calcium Dependent Protein Kinases

scholarly journals Calcium-dependent protein kinases in cotton: insights into early plant responses to salt stress

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Wei Gao ◽  
Fu-Chun Xu ◽  
Dan-Dan Guo ◽  
Jing-Ruo Zhao ◽  
Ji Liu ◽  
...  
Keyword(s):  
Salt Stress ◽  
Protein Kinases ◽  
Plant Responses ◽  
Calcium Dependent ◽  
Dependent Protein ◽  
Calcium Dependent Protein Kinases

Calcium-Dependent Protein Kinases (CDPK) in Abiotic Stress Tolerance

2018 ◽  
Vol 34 (2) ◽  
pp. 259-265 ◽  
Author(s):  
Hemant B Kardile ◽  
◽  
Vikrant ◽  
Nirmal Kant Sharma ◽  
Ankita Sharma ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Stress Tolerance ◽  
Protein Kinases ◽  
Abiotic Stress Tolerance ◽  
Calcium Dependent ◽  
Dependent Protein ◽  
Calcium Dependent Protein Kinases

scholarly journals Exploring Ruthenium-based organometallic inhibitors against Plasmodium Calcium Dependent Kinase 2 (PfCDPK2): a combined ensemble docking, QM paramterization and molecular dynamics study

2020 ◽  
Author(s):  
Dhaval Patel ◽  
Mohd Athar ◽  
Prakash C. Jha
Keyword(s):  
Molecular Dynamics ◽  
Metal Organic Framework ◽  
Calcium Dependent ◽  
Ensemble Docking ◽  
Metal Organic ◽  
Dependent Protein ◽  
Calcium Dependent Protein Kinases ◽  
Dynamics Simulations ◽  
The Given ◽  
Computational Drug Discovery

ABSTRACTRecent advances in the metal-organic framework (MOF) have accelerated the discovery of novel metal-based anticancer, antibacterial and antimalarial compounds. This is substantiated by many serendipitously discovered metals (Ru, Rh, and Ir) based inhibitors that established the importance of metal inserted into the known organic scaffold. Conversely, it is possible to design novel bioactive compounds by mimicking hypervalent carbon atoms by transition metals. This process can be facilitated by computational drug discovery by treating metal center using optimized parameters that can be used for molecular docking and molecular dynamics simulations. Further, the method can be plugged with high computational power and refined algorithms to interpret chemical phenomena with atomic-level insights. In the present work, we have demonstrated an approach for parameterizing three organometallic ligands (FLL, E52, and staurosporine) using MCPB.py. In particular, we report that E52 and FLL have a better shape complimentary and affinity compared to staurosporine identified inhibitor (staurosporine) against Calcium-dependent protein kinases 2 (CDPK2). This study also revealed that a flexible approach (ensemble) outperforms for the given target with dynamic movements. The calculated MMPBSA energies for staurosporine, FLL and E52 were −66.461 ± 2.192, −67.182 ± 1.971 and −91.339 ± 2.745 kcal/mol respectively.

Sign in / Sign up

Export Citation Format

Share Document