Effect of Light Irradiation on Plant Production in Large-Scale Plant Tissue Culture

Abstract Plant tissue culture (micropropagation) is the aseptic culture of cells, tissues, organs and their components under controlled conditions in vitro where the environment and nutrition are rigidly controlled. While tissue culture is the most commonly applied and widely recognised term for this process, micropropagation, in vitro culture, sterile culture and axenic culture may also be used (Smith, 2013). Tissue culture has developed to the point where it has become an important tool in both basic and applied studies, as well as in commercial application and large scale plant production (Thorpe, 2007). The first attempts at culturing isolated plant cells in vitro on artificial medium were carried out by the German scientist, Gottlieb Haberlandt in 1902 (Krikorian and Berquam, 1969). While these early experiments were unsuccessful, Haberlandt proposed and established the new concept of 'totipotency' - the potential of a plant cell to grow and develop into a whole new multicellular plant via differentiation of a single cell into many other cell types. Thus Haberlandt is justifiably recognised as the father of plant tissue culture (Thorpe, 2007). By 1922 further studies by Robbins and Kottle had established short-term cultures of isolated root tips. By 1934 other researchers began to build on this process and maintained indefinite culture of tomato root tips (White, 1934). During the 1930s and 1940s more important findings added to the initial process of cell culturing with the first long-term plant tissue culture of callus from explants of cambial tissue isolated from carrot carried out by Gautheret and Nobecourt. This was followed by callus culture of tobacco tumour tissue which was induced to differentiate into roots and shoots (White, 1939).

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Transient expression of GFP in plant tissue culture in vitro using viral-based module system

Faktori eksperimental'noi evolucii organizmiv ◽

10.7124/feeo.v20.763 ◽

1970 ◽

pp. 198-201

Author(s):

O. M. Kishchenko ◽

A. A. Peterson ◽

M. Yu. Vasylenko ◽

M. V. Kuchuk

Keyword(s):

Tissue Culture ◽

Transient Expression ◽

Plant Tissue Culture ◽

Plant Tissue ◽

Expression System ◽

Polyacrylamide Gel Electrophoresis ◽

Plant Production ◽

Culture In Vitro ◽

Module System

Aim. Agrobacterium-mediated transient expression using viral-based vectors is one of the most effective method for recombinant protein production in plant. Transient expression of GFP using viral-based module system was studied in plant tissue culture in vitro. Methods. Regenerated shoots and callus clones of Nicotiana benthamiana were agroinfiltrated with viral-based module system. Protein extracts from GFP-positive tissues were resolved by non-reducing polyacrylamide gel electrophoresis. GFP from gel was eluted and GFP fluorescence was measured by fluorescence spectrometry with excitation filter at 395 nm and emission filter at 510 nm. Results. Regenerated shoots and callus clones lines accumulated GFP at 242.0±0.7 and 221.6±4.1 μg per g fresh tissue, respectively. The obtained level of transient expression is comparable with other plant production systems in early stage development. Conclusions. The developed technique shows promise for production of therapeutic proteins and antigens in the short term (14–16 days) by transient expression system in plant tissue culture in vitro. Keywords: viral-based module system, transient expression, recombinant proteins, plant tissue culture, GFP.

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A practical method for large-scale plant tissue culture

Experimental Cell Research ◽

10.1016/s0014-4827(66)80102-7 ◽

1966 ◽

Vol 41 (3) ◽

pp. 509-520 ◽

Cited By ~ 25

Author(s):

J.E. Graebe ◽

G.D. Novelli

Keyword(s):

Tissue Culture ◽

Plant Tissue Culture ◽

Plant Tissue ◽

Large Scale ◽

Practical Method

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Large-Scale Plant Tissue Culture

Agricultural Biotechnology - Books in Soils, Plants, and the Environment ◽

10.1201/9781420049275.ch9 ◽

1997 ◽

pp. 225-249

Author(s):

A Scragg

Keyword(s):

Tissue Culture ◽

Plant Tissue Culture ◽

Plant Tissue ◽

Large Scale

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Plant Tissue Culture for In Vitro Mutagenesis, Large-Scale Propagation, and Genetic Transformation

Plant Tissue Culture: Propagation, Conservation and Crop Improvement ◽

10.1007/978-981-10-1917-3_14 ◽

2016 ◽

pp. 309-342 ◽

Cited By ~ 2

Author(s):

Pratibha Misra ◽

Syed Saema

Keyword(s):

Tissue Culture ◽

Genetic Transformation ◽

Plant Tissue Culture ◽

Plant Tissue ◽

Large Scale ◽

In Vitro Mutagenesis

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TISSUE CULTURE AS A PLANT PRODUCTION TECHNIQUE FOR FRUIT CROPS AND PLANT PART USED FOR PROPAGATION

International Journal on Agricultural Sciences ◽

10.53390/ijas.v11i2.5 ◽

2020 ◽

Vol 11 (2) ◽

Author(s):

Rahul R. Rodge ◽

Pravin Patil

Keyword(s):

Tissue Culture ◽

Plant Tissue Culture ◽

Plant Tissue ◽

Small Degree ◽

Plant Production ◽

Whole Plant ◽

Commercial Technology ◽

Micro Propagation

The culture of plant tissue refers to the growth and multiplication in aseptic and regulated environments of cells, tissues and plant organs on specified solid or liquid media. In the fields of agriculture, horticulture, forestry and plant breeding, plant tissue culture has been commonly employed. it is an applied biotechnology used for mass proliferation, extinction of viruses, development of secondary metabolites and In-vitro cloning of plants. Plant tissue culture, slow growth and cryopreservation, also known as long-term preservation are also known as, has been extensively used for the preservation of endangered plant species., through short and medium-term conservation. In a suitable culture medium, a whole plant may be regenerated from a small tissue or plant cell. under controlled environment. Commercial technology is mostly based on micro propagation, where rapid proliferation is performed from process cuttings, axillary buds, somatic embryos, cell clumps in suspension cultures, and bioreactors to a small degree.

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