scholarly journals Anthocyanins Profile Characterization of Common Grape Hyacinth (Muscari neglectum Guss. ex Ten.) Flowers Growing in Hungary by High-performance Liquid Chromatography Coupled to Diode Array Detector and Mass Spectrometry using the Electrospray Ionization Interface

2020 ◽  
Vol 11 (01) ◽  
pp. 15-19
Author(s):  
Dmitry Olegovich Bokov ◽  
Eszter Riethmüller
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Electrospray Ionization ◽  
High Performance ◽  
Ion Source ◽  
Array Detector ◽  
Diode Array ◽  
Tandem Mass Spectrometric ◽  
Grape Hyacinth

Objective: Anthocyanins are one of the biologically active substances group playing an important role in the state of physiological functions referring to human health. This research aimed to investigate the anthocyanins profiles in the grape hyacinth (Muscari neglectum Guss. ex Ten.). Materials and methods: The identification of individual anthocyanins was carried using the method of high-performance liquid chromatography with diode array detection and mass spectrometry with electrospray ionization (DAD-ESI-MS) analysis. Chromatographic separation and tandem mass spectrometric analyses were performed on an Agilent 1100 HPLC system and Agilent 6410 triple quadrupole system equipped with an electrospray ion source (ESI) in positive ion mode. Results: In the M. neglectum flowers (tepals), nine individual anthocyanins, containing delphinidin, petunidin, malvidine, pelargonidin aglycones were found. In this research, we report anthocyanin profiles for the M. neglectum flowers for the first time. Conclusion: The obtained results concerning anthocyanins composition may be very useful for researchers in the field of the standardization and activity evaluation of extracts produced from M. neglectum.

scholarly journals Quantification of Antioxidant Phenolic Compounds in a New Chrysanthemum Cultivar by High-Performance Liquid Chromatography with Diode Array Detection and Electrospray Ionization Mass Spectrometry

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Ah-Reum Han ◽  
Hyo Young Kim ◽  
Yangkang So ◽  
Bomi Nam ◽  
Ik-Soo Lee ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Electrospray Ionization ◽  
Electrospray Ionization Mass Spectrometry ◽  
High Performance ◽  
Ionization Mass Spectrometry ◽  
Array Detector ◽  
Diode Array ◽  
Ionization Mass

The flowers of Chrysanthemum morifolium Ramat. have been used as an herbal tea and in traditional medicine, and the plant has been developed to produce horticultural cultivars of various colors and shapes. In this study, a new chrysanthemum cultivar with dark purple petals (C. morifolium cv. ARTI-Dark Chocolate; ADC) was developed by radiation-induced mutation breeding of its original cultivar with purple striped white petals (C. morifolium cv. Noble Wine, NW). The phenolic profile and antioxidant property of ADC were investigated and compared with NW and the commercially available medicinal herb, C. morifolium with yellow petals (CM), in order to find a scientific support to produce a new source of natural antioxidant. Flavonoid and phenolic acid profiles of the ethanol extracts of the three flowers were analyzed by high-performance liquid chromatography-diode array detector-electrospray ionization mass spectrometry (HPLC-DAD-ESIMS), while antioxidant properties were evaluated using the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging assay. Among the tested flowers, ADC possessed the strongest antioxidant capacity and the highest phenolic contents. Flavonoids (acacetin, apigenin, luteolin, acacetin-7-O-β-glucoside, apigenin-7-O-β-glucoside, luteolin-7-O-β-glucoside, and linarin) and phenolic acids (chlorogenic acid and mixture of 1,4-, 1,5-, and 3,5-dicaffeoylquinic acids) were identified and quantified.

scholarly journals Simultaneous Extraction Optimization and Analysis of Flavonoids from the Flowers of Tabernaemontana heyneana by High Performance Liquid Chromatography Coupled to Diode Array Detector and Electron Spray Ionization/Mass Spectrometry

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Thiyagarajan Sathishkumar ◽  
Ramakrishnan Baskar ◽  
Mohan Aravind ◽  
Suryanarayanan Tilak ◽  
Sri Deepthi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Shake Flask ◽  
Orthogonal Design ◽  
Array Detector ◽  
Diode Array ◽  
Ionization Mass ◽  
Diode Array Detector

Flavonoids are exploited as antioxidants, antimicrobial, antithrombogenic, antiviral, and antihypercholesterolemic agents. Normally, conventional extraction techniques like soxhlet or shake flask methods provide low yield of flavonoids with structural loss, and thereby, these techniques may be considered as inefficient. In this regard, an attempt was made to optimize the flavonoid extraction using orthogonal design of experiment and subsequent structural elucidation by high-performance liquid chromatography-diode array detector-electron spray ionization/mass spectrometry (HPLC-DAD-ESI/MS) techniques. The shake flask method of flavonoid extraction was observed to provide a yield of 1.2±0.13 (mg/g tissue). With the two different solvents, namely, ethanol and ethyl acetate, tried for the extraction optimization of flavonoid, ethanol (80.1 mg/g tissue) has been proved better than ethyl acetate (20.5 mg/g tissue). The optimal conditions of the extraction of flavonoid were found to be 85°C, 3 hours with a material ratio of 1 : 20, 75% ethanol, and 1 cycle of extraction. About seven different phenolics like robinin, quercetin, rutin, sinapoyl-hexoside, dicaffeic acid, and two unknown compounds were identified for the first time in the flowers of T. heyneana. The study has also concluded that L16 orthogonal design of experiment is an effective method for the extraction of flavonoid than the shake flask method.

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