Preliminary Phytochemical Analysis of Extract of Spermadictyon suaveolens and its Effect on Oral Glucose Tolerance in Streptozotocin
Induced Diabetic Rats

The preliminary phytochemical analysis of extract of Spermadictyon suaveolens was performed and its effect on oral glucose tolerance in streptozotocin induced diabetic rats studied. 1 week after the administration of streptozotocin, diabetic rats received herbal extract orally at dose 500 mg/kg body weight for 28 consecutive days. Oral glucose tolerance test was performed on overnight fasted rats on day 28. Blood samples were collected prior to glucose administration and at 30, 60, 90, 120 minutes after glucose loading and plasma glucose level was measured for all the samples. The preliminary phytochemical analysis of extract of roots of Spermadictyon suaveolens showed the presence of carbohydrates, flavonoids, glycosides, tannins and phenolic compounds. Extract of roots of Spermadictyon suaveolens improved oral glucose tolerance in streptozotocin induced diabetic rats.

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Pharmacological potential of methanol extract of Anacardium occidentale stem bark on alloxan-induced diabetic rats

Biomedical Research and Therapy ◽

10.15419/bmrat.v5i7.456 ◽

2018 ◽

Vol 5 (7) ◽

pp. 2440-2454

Author(s):

D. A. Omoboyowa ◽

F. O. Afolabi ◽

T. C. Aribigbola

Keyword(s):

Blood Glucose ◽

Glucose Tolerance ◽

Glucose Tolerance Test ◽

Methanol Extract ◽

Stem Bark ◽

Tolerance Test ◽

Diabetic Rats ◽

Oral Glucose ◽

Oral Glucose Tolerance ◽

Β Cells

Background: The anti-hyperglycemic potential of methanol stem bark extract of Anacardium occidentale (MSBEAO) was investigated using an alloxan-induced diabetic rat model. Alloxan administration induces the generation of free radicals which can affect antioxidant status resulting in the disruption of the β-cells of the pancreas. Therefore, this study examines the antioxidant potential of the plant extract and the ameliorating effect on the pancreas of alloxan-induced diabetic rats. Methods: Diabetes was induced by intraperitoneal injection of 150 mg/kg body weight of alloxan monohydrate. MSBEAO, at a concentration of 100 or 200 mg/kg b.w. was orally administered to alloxan-induced diabetic rats and normal rats. The hypoglycemic effect, oral glucose tolerance test, and biochemical assay of alloxan-induced diabetic rats were assayed using standard procedures. Results: Preliminary phytochemical screening of the extract revealed the presence of alkaloids, tannins, saponins, terpenoids, carbohydrates, and phenols at moderate concentrations. The lethality dose (LD50) of the plant extract was found to be equal to or less than 5000 mg/kg b.w. The hypoglycemic effect of the extract on the non-diabetic rats revealed a significant (p<0.05) decrease in the blood glucose concentration of animals administered with 1 g/kg b.w. of the extract, compared to normal control rats administered with normal saline. In the oral glucose tolerance test, the methanol extract exerted the highest response, similar to glibenclamide after 15 and 30 minutes of administration, compared to the control rats. The methanol extract yielded the highest blood glucose lowering effects after 9 days of treatment (p<0.05), compared to diabetic rats administered with normal saline and 0.3 mg/kg b.w. of glibenclamide. Administration of the extract at 200 mg/kg b.w. showed improved pancreas architecture and regeneration of the β-cells, compared with the pancreas of animals in the other groups. Conclusion: The results of this study suggest that MSBEAO is a potentially effective agent for the management of diabetes which might result from the antioxidant-generating capacity of the stem bark.

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Effects of aqueous leaf extract of Ocimum gratissimum on oral glucose tolerance test in type-2 model diabetic rats

African Journal of Pharmacy and Pharmacology ◽

10.5897/ajpp11.811 ◽

2012 ◽

Vol 6 (9) ◽

Author(s):

NELSON I. OGUANOBI

Keyword(s):

Glucose Tolerance ◽

Oral Glucose Tolerance Test ◽

Leaf Extract ◽

Glucose Tolerance Test ◽

Tolerance Test ◽

Diabetic Rats ◽

Oral Glucose ◽

Oral Glucose Tolerance ◽

Aqueous Leaf Extract

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Oral administration of sodium tungstate improves cardiac performance in streptozotocin-induced diabetic rats

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y05-026 ◽

2005 ◽

Vol 83 (5) ◽

pp. 405-411 ◽

Cited By ~ 16

Author(s):

Prabhakara Reddy Nagareddy ◽

Harish Vasudevan ◽

John H McNeill

Keyword(s):

Glucose Tolerance ◽

Oral Glucose Tolerance Test ◽

Glucose Tolerance Test ◽

Sodium Tungstate ◽

Tolerance Test ◽

Diabetic Rats ◽

Cardiac Performance ◽

Oral Glucose ◽

Oral Glucose Tolerance ◽

Working Heart

Normalization of hyperglycemia and hyperlipidemia is an important objective in preventing diabetes-induced cardiac dysfunction. Our study investigated the effects of sodium tungstate on cardiac performance in streptozotocin-induced (STZ) diabetic rats based on its potential antidiabetic and antioxidant activity. Male Wistar rats were made STZ-diabetic and then treated with tungstate in their drinking water for 9 weeks. Body mass, food and fluid intake, plasma glucose, insulin, triglyceride, and free fatty acids levels were measured. At the termination of the study period, an oral glucose tolerance test (OGTT) was performed, and cardiac performance was evaluated using an isolated working heart apparatus. Tungstate-treated STZ-diabetic rats showed a significant reduction in fluid and food intake, plasma glucose, triglycerides, and free fatty acid levels, and improved tolerance to glucose in OGTT, owing to tungstate-mediated enhancement of insulin activity rather than increased insulin levels. Left ventricular pressure development, the rate of contraction (+dP/dT), and the rate of relaxation (-dP/dT) were significantly improved in tungstate-treated diabetic rats. Apart from a decreased rate of body mass gain, no other signs of toxicity or hypoglycemic episodes were observed in tungstate-treated rats. This study extends previous observations on the antidiabetic activities of tungstate, and also reports for the first time the salutary effects in preventing diabetic cardiomyopathy.Key words: diabetes, cardiomyopathy, tungstate, isolated working heart, oral glucose tolerance test.

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