scholarly journals PENGARUH PERLAKUAN PENDAHULUAN DENGAN KULTUR CAMPURAN JAMUR PELAPUK PUTIH Phanerochaete crysosporium, Pleurotus ostreatus DAN Trametes versicolor TERHADAP KADAR LIGNIN DAN SELULOSA BAGAS

2016 ◽  
Vol 1 (02) ◽  
Author(s):  
Sita Heris Anita ◽  
Euis Hermiati ◽  
Raden Permana Budi Laksana
Keyword(s):  
Lignocellulosic Biomass ◽  
Pleurotus Ostreatus ◽  
Trametes Versicolor ◽  
Lignin Degradation ◽  
White Rot Fungi ◽  
White Rot ◽  
Cellulose Content ◽  
Biological Pretreatment ◽  
Physico Chemical ◽  
Before And After

Conversion of lignocellulosic biomass requires pretreatment in order to separate lignin from cellulose. Several methods have been introduced for pretreatment process of lignocellulosic biomass. These are physical, physico-chemical, chemical, as well as biological pretreatment. The following investigation aimed at a biological pretreatment of bagasse using mix-culture of white-rot fungi with the objective to investigate pretreatment effect on the lignin degradation of bagasse with minimum loss of cellulose content. The white-rot fungi involved Phanerochaete crysosporium (Pc), Pleurotus ostreatus (Po), and Trametes versicolor (Tv) on degrading lignin of bagasse with minimal losses of cellulose content 5% (w/v). The culture variation are as follows Po dan Tv (1:1), Po dan Pc (1:1), Pc dan Tv (1:1) and Po, Pc dan Tv (1:1:1) they were inoculated into steamed bagasse and incubated for 1, 2, 3, and 4 weeks respectively. Chemical analysis was done to determine the weight loss, lignin, and alphacellulose content before and after pretreatment. Optimum lignin degradation was achieved after two weeks incubation for each mix-culture. The most effective lignin degradation of mix-culture Po, Pc and Tv (25,26±1,57%) with minimum cellulose loss (17,27±3,76%).Key words : pretreatment, white-rot fungi, mix-culture, baggaseINTISARIKonversi biomassa lignoselulosa membutuhkan proses perlakuan pendahuluan yang bertujuan untuk memisahkan lignin dari selulosa. Beberapa metode telah banyak diketahui untuk proses perlakuan pendahuluan biomassa lignoselulosa, diantaranya perlakuan pendahuluan secara fisika, fisika-kimia, kimia dan biologi. Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan kultur campur jamur pelapuk putih Phanerochaete crysosporium (Pc), Pleurotus ostreatus (Po), dan Trametes versicolor (Tv) terhadap penurunan kadar lignin bagas dengan kehilangan minimal α-selulosa. Inokulum yang digunakan sebesar 5% (w/v) dengan variasi kultur Po dan Tv (1:1), Po dan Pc (1:1), Pc dan Tv (1:1) dan Po, Pc dan Tv (1:1:1). Kultur campur jamur diinokulasikan ke dalam bagas dan diinkubasi masing-masing selama 1, 2, 3, dan 4 minggu. Analisa kimia dilakukan untuk mengetahui kehilangan berat, kadar lignin serta selulosa bagas sebelum dan setelah perlakuan pendahuluan. Hasil penelitian menunjukkan bahwa penurunan optimal kadar lignin bagas dengan kehilangan minimal α-selulosa terjadi pada waktu inkubasi dua minggu untuk setiap variasi kultur campur. Dari empat variasi kultur campur pada waktu inkubasi dua minggu menunjukkan bahwa kehilangan rata-rata lignin bagas tertinggi (325,26±1,57%) dengan kehilangan minimal α-selulosa (17,27±3,76%) terjadi pada kultur campur Pc dan Tv.Kata kunci : perlakuan pendahuluan, jamur pelapuk putih, kultur campur, bagas  

scholarly journals Characteristic of oil palm empty fruit bunch pretreated with Pleurotus floridanus (Pretreatment biologi tandan kosong kelapa sawit menggunakan Pleurotus floridanus)

2017 ◽  
Vol 85 (2) ◽  
Author(s):  
. ISROI
Keyword(s):  
Oil Palm ◽  
Lignin Degradation ◽  
Lignin Content ◽  
White Rot Fungi ◽  
Ligninolytic Enzyme ◽  
White Rot ◽  
Cellulose Content ◽  
Biological Pretreatment ◽  
Empty Fruit Bunches ◽  
Pleurotus Floridanus

Pleurotus floridanus have ability on lignin degradation by producing ligninolytic enzyme and prefer to degrade lignin than carbohydrate (hemicellulose and cellulose). Oil palm empty fruit bunches has been pretreated using P. floridanus.  Addition of cation (Cu2+) on biological pretreatment reduced lignin content and increased digestibility of the empty fruit bunches. P. floridanus reduce lignin and hemicellulose content from 23.9% to 10.1% and from 20.8% to 16.9%, respectively. P. floridanus did not degrade cellulose. Cellulose content of empty fruit bunches increase from 40.4% to 51.7%. Crystallinity of empty fruit bunches reduced after biological pretreatment. Crystallinity presented as LOI (lateral order index) of un-treated and biological pretreated oil palm empty fruit bunches are 2.08 and 1.44. Digestibility of the empty fruit bunches increased from 17.2% to 60.3% by biological pretreatment.[Key words:  biological pretreatment, oil palm empty fruit bunches, Pleurotus floridanus, biofuel, white-rot fungi, lignocellulose]AbstrakPleurotus floridanus memiliki kemampuan untuk mendegradasi lignin dengan memproduksi enzim ligninolitik dan lebih memilih untuk mendegradasi lignin daripada karbohidrat (hemiselulosa dan selulosa). Kemampuan unik P. floridanus ini dimanfaatkan dalam pretreatment biologi tandan kosong kelapa sawit. Penambahan kation (Cu2+) pada pretreatment biologi menurunkan kandungan lignin dan meningkatkan digestibiliti tandan kosong kelapa sawit. Perlakuan P. floridanus mengurangi kandungan lignin dan hemiselulosa dari 23,9% menjadi 10,1% dan dari 20,8% menjadi 16,9%. Perlakuan P. floridanus tidak menurunkan kandungan selulosa. Kandungan selulosa tandan kosong kelapa sawit meningkat dari 40,4% menjadi 51,7%. Kristalinitas tandan kosong menurun setelah pretreatment biologi. Kristalinitas yang dinyatakan dalam LOI (LOI, Lateral Order Index) adalah 2,08 untuk tandan kosong tanpa pretreatment biologi dan 1,44 untuk tandan kosong dengan pretreatment biologi. Digestibiliti itandan kosong meningkat dari 17,2% menjadi 60,3%.[Kata kunci: Pretreatment biologi, tandan kosong kelapa sawit, jamur pelapuk putih, lignoselulosa, Pleurotus floridanus]

Comparison between degradation capabilities of the white rot fungi Pleurotus ostreatus and Trametes versicolor in beech wood

2015 ◽  
Vol 104 ◽  
pp. 231-237 ◽  
Author(s):  
Ehsan Bari ◽  
Nouredin Nazarnezhad ◽  
Seyed Mahmoud Kazemi ◽  
Mohammad Ali Tajick Ghanbary ◽  
Behbood Mohebby ◽  
...  
Keyword(s):  
Pleurotus Ostreatus ◽  
Trametes Versicolor ◽  
Beech Wood ◽  
White Rot Fungi ◽  
White Rot

scholarly journals Use of white rot fungi in the degradation of an azo dye from the textile industry1

DYNA ◽  
2016 ◽  
Vol 83 (198) ◽  
pp. 128 ◽  
Author(s):  
Ana Zuleta-Correa ◽  
Andrés Merino-Restrepo ◽  
Angelina Hormaza-Anaguano ◽  
Santiago Alonso Cardona Gallo
Keyword(s):  
Pleurotus Ostreatus ◽  
Azo Dye ◽  
Trametes Versicolor ◽  
White Rot Fungi ◽  
White Rot ◽  
Pleurotus Pulmonarius

Los efluentes de la industria textil imponen grandes retos ambientales. Estos son una mezcla compleja de productos químicos, donde colorantes son de particular interés. En este estudio, se implementó un diseño factorial completo 23 para establecer las condiciones más apropiadas para la degradación del rojo básico 46 bajo fermentación en estado sólido, FES. Tres hongos de podredumbre blanca Trametes versicolor, Pleurotus ostreatus y Pleurotus pulmonarius fueron utilizados en el proceso de fermentación. Se alcanzó una degradación máxima de 63.0% a los 17 días de incubación con T. versicolor con una humedad del 90%, proporción carbono:nitrógeno de 12:1 y a 20°C. P. ostreatus y P. pulmonarius alcanzaron porcentajes máximos de degradación del 69.3% y 63.1% respectivamente luego de 25 días de fermentación. El escalado del proceso con T. versicolor condujo a una degradación del 45.7% a los 30 días de incubación. Adicionalmente, se cuantificó la actividad de las enzimas lacasa, manganeso peroxidasa y lignina peroxidasa. Los resultados señalan que la FES ofrece una degradación satisfactoria, cuya eficiencia depende de la optimización de las condiciones del proceso.

scholarly journals Biopulp from Pineapple Leaf Fiber Produced by Colonization with Two White-Rot Fungi: Trametes versicolor and Pleurotus ostreatus

BioResources ◽  
2016 ◽  
Vol 11 (4) ◽  
Author(s):  
Róger Moya ◽  
Alexander Berrocal ◽  
Ana Rodríguez-Zúñiga ◽  
María Rodriguez-Solis ◽  
Verónica Villalobos-Barquero ◽  
...  
Keyword(s):  
Pleurotus Ostreatus ◽  
Trametes Versicolor ◽  
White Rot Fungi ◽  
White Rot ◽  
Pineapple Leaf Fiber ◽  
Leaf Fiber

Enhanced Lignin Degradation in Tobacco Stalk Composting with Inoculation of White-Rot Fungi Trametes hirsuta and Pleurotus ostreatus

2019 ◽  
Vol 11 (7) ◽  
pp. 3525-3535 ◽  
Author(s):  
Liang Yang ◽  
Honglun Yuan ◽  
Yong Yang ◽  
Rui Wang ◽  
Changjun Wang ◽  
...  
Keyword(s):  
Pleurotus Ostreatus ◽  
Lignin Degradation ◽  
White Rot Fungi ◽  
White Rot ◽  
Trametes Hirsuta ◽  
Tobacco Stalk

scholarly journals Screening of ligninolytic fungi for biological pretreatment of lignocellulosic biomass

2015 ◽  
Vol 61 (10) ◽  
pp. 745-752 ◽  
Author(s):  
Chunyan Xu ◽  
Deepak Singh ◽  
Kathleen M. Dorgan ◽  
Xiaoyu Zhang ◽  
Shulin Chen
Keyword(s):  
Lignocellulosic Biomass ◽  
Peroxidase Activity ◽  
Low Cost ◽  
White Rot Fungi ◽  
High Potential ◽  
White Rot ◽  
Barley Straw ◽  
Laccase Production ◽  
Biological Pretreatment ◽  
Ligninolytic Fungi

To identify white rot fungi with high potential in biological pretreatment of lignocellulosic biomass, preliminary screening was carried out on plates by testing different strains for their ability to oxidize guaiacol and decolorize the dyes azure B and Poly R-478. Of the 86 strains screened, 16 were selected for secondary screening for their ligninolytic ability; however, low manganese peroxidase activity and no lignin peroxidase activity were detected. Strain BBEL0970 proved to be the most efficient in laccase production and was subsequently identified as Trametes versicolor by analysis of the ribosomal DNA internal transcribed spacer gene sequence. In combining laccase production with biological pretreatment, the replacement of glucose with barley straw significantly improved the laccase activity by up to 10.3 U/mL, which provided evidence toward potential utilization of barley straw in laccase production by BBEL0970. Simultaneously, comparison by thermogravimetric analysis of the untreated and pretreated barley straw in liquid fermentation of laccase also demonstrated the high potential of BBEL0970 in biological pretreatment of lignocellulosic biomass. This work sheds light on further exploration on the integrated process of low-cost laccase production and efficient biological pretreatment of barley straw by T. versicolor BBEL0970.

Effect of an electro-Fenton process on the biodegradation of lignin by Trametes versicolor

BioResources ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. 8039-8050
Author(s):  
Jiahe Shen ◽  
Lipeng Hou ◽  
Haipeng Sun ◽  
Mingyang Hu ◽  
Lihua Zang ◽  
...  
Keyword(s):  
Hydroxyl Radicals ◽  
Manganese Peroxidase ◽  
Trametes Versicolor ◽  
Lignin Degradation ◽  
White Rot Fungi ◽  
Maximum Growth ◽  
White Rot ◽  
Lignin Biodegradation ◽  
Fenton Process ◽  
Mycelium Growth

A synergistic effect was found between the electro-Fenton (E-Fenton) process and a white-rot enzyme (Trametes versicolor) system relative to the degradation of dealkaline lignin. The hydrogen peroxide produced by the E-Fenton process reacted with Fe2+ on the cathode to generate a large number of hydroxyl radicals. These hydroxyl radicals directly degraded various functional groups in lignin, which led to the quick initiation of lignin peroxidase (LiP) and manganese peroxidase (MnP) enzymatic hydrolysis and accelerated the progress of lignin biodegradation. In addition, the hydroxyl radicals produced by the Fenton reaction converted nonphenolic lignin into phenolic lignin, further promoting the ability of manganese peroxidase and laccase to degrade the lignin. Additionally, the Fe3+ secreted by white-rot fungi accelerated the regeneration of Fe2+ on the composite cathode, which sustained the lignin degradation system. In the synergistic system, mycelium growth was significantly improved, with the maximum growth amount reaching 2.3 g and the lignin degradation rate reaching 84.5%, the activity of the three enzymes increased with the increase of currents over 96 h. Among them, the activity of MnP increased significantly to 402 U/L.

Degradation of a binary mixture of synthetic dyes by the white-rot fungi Pleurotus ostreatus and Trametes versicolor

2016 ◽  
Vol 33 ◽  
pp. S137
Author(s):  
Andrés Merino Restrepo ◽  
Angelina Hormaza Anaguano ◽  
Carolina Velásquez Quintero
Keyword(s):  
Binary Mixture ◽  
Pleurotus Ostreatus ◽  
Trametes Versicolor ◽  
White Rot Fungi ◽  
White Rot ◽  
Synthetic Dyes
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