Comparison between degradation capabilities of the white rot fungi Pleurotus ostreatus and Trametes versicolor in beech wood

2015 ◽  
Vol 104 ◽  
pp. 231-237 ◽  
Author(s):  
Ehsan Bari ◽  
Nouredin Nazarnezhad ◽  
Seyed Mahmoud Kazemi ◽  
Mohammad Ali Tajick Ghanbary ◽  
Behbood Mohebby ◽  
...  
2016 ◽  
Vol 1 (02) ◽  
Author(s):  
Sita Heris Anita ◽  
Euis Hermiati ◽  
Raden Permana Budi Laksana

Conversion of lignocellulosic biomass requires pretreatment in order to separate lignin from cellulose. Several methods have been introduced for pretreatment process of lignocellulosic biomass. These are physical, physico-chemical, chemical, as well as biological pretreatment. The following investigation aimed at a biological pretreatment of bagasse using mix-culture of white-rot fungi with the objective to investigate pretreatment effect on the lignin degradation of bagasse with minimum loss of cellulose content. The white-rot fungi involved Phanerochaete crysosporium (Pc), Pleurotus ostreatus (Po), and Trametes versicolor (Tv) on degrading lignin of bagasse with minimal losses of cellulose content 5% (w/v). The culture variation are as follows Po dan Tv (1:1), Po dan Pc (1:1), Pc dan Tv (1:1) and Po, Pc dan Tv (1:1:1) they were inoculated into steamed bagasse and incubated for 1, 2, 3, and 4 weeks respectively. Chemical analysis was done to determine the weight loss, lignin, and alphacellulose content before and after pretreatment. Optimum lignin degradation was achieved after two weeks incubation for each mix-culture. The most effective lignin degradation of mix-culture Po, Pc and Tv (25,26±1,57%) with minimum cellulose loss (17,27±3,76%).Key words : pretreatment, white-rot fungi, mix-culture, baggaseINTISARIKonversi biomassa lignoselulosa membutuhkan proses perlakuan pendahuluan yang bertujuan untuk memisahkan lignin dari selulosa. Beberapa metode telah banyak diketahui untuk proses perlakuan pendahuluan biomassa lignoselulosa, diantaranya perlakuan pendahuluan secara fisika, fisika-kimia, kimia dan biologi. Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan kultur campur jamur pelapuk putih Phanerochaete crysosporium (Pc), Pleurotus ostreatus (Po), dan Trametes versicolor (Tv) terhadap penurunan kadar lignin bagas dengan kehilangan minimal α-selulosa. Inokulum yang digunakan sebesar 5% (w/v) dengan variasi kultur Po dan Tv (1:1), Po dan Pc (1:1), Pc dan Tv (1:1) dan Po, Pc dan Tv (1:1:1). Kultur campur jamur diinokulasikan ke dalam bagas dan diinkubasi masing-masing selama 1, 2, 3, dan 4 minggu. Analisa kimia dilakukan untuk mengetahui kehilangan berat, kadar lignin serta selulosa bagas sebelum dan setelah perlakuan pendahuluan. Hasil penelitian menunjukkan bahwa penurunan optimal kadar lignin bagas dengan kehilangan minimal α-selulosa terjadi pada waktu inkubasi dua minggu untuk setiap variasi kultur campur. Dari empat variasi kultur campur pada waktu inkubasi dua minggu menunjukkan bahwa kehilangan rata-rata lignin bagas tertinggi (325,26±1,57%) dengan kehilangan minimal α-selulosa (17,27±3,76%) terjadi pada kultur campur Pc dan Tv.Kata kunci : perlakuan pendahuluan, jamur pelapuk putih, kultur campur, bagas  


DYNA ◽  
2016 ◽  
Vol 83 (198) ◽  
pp. 128 ◽  
Author(s):  
Ana Zuleta-Correa ◽  
Andrés Merino-Restrepo ◽  
Angelina Hormaza-Anaguano ◽  
Santiago Alonso Cardona Gallo

Los efluentes de la industria textil imponen grandes retos ambientales. Estos son una mezcla compleja de productos químicos, donde colorantes son de particular interés. En este estudio, se implementó un diseño factorial completo 23 para establecer las condiciones más apropiadas para la degradación del rojo básico 46 bajo fermentación en estado sólido, FES. Tres hongos de podredumbre blanca Trametes versicolor, Pleurotus ostreatus y Pleurotus pulmonarius fueron utilizados en el proceso de fermentación. Se alcanzó una degradación máxima de 63.0% a los 17 días de incubación con T. versicolor con una humedad del 90%, proporción carbono:nitrógeno de 12:1 y a 20°C. P. ostreatus y P. pulmonarius alcanzaron porcentajes máximos de degradación del 69.3% y 63.1% respectivamente luego de 25 días de fermentación. El escalado del proceso con T. versicolor condujo a una degradación del 45.7% a los 30 días de incubación. Adicionalmente, se cuantificó la actividad de las enzimas lacasa, manganeso peroxidasa y lignina peroxidasa. Los resultados señalan que la FES ofrece una degradación satisfactoria, cuya eficiencia depende de la optimización de las condiciones del proceso.


BioResources ◽  
2016 ◽  
Vol 11 (4) ◽  
Author(s):  
Róger Moya ◽  
Alexander Berrocal ◽  
Ana Rodríguez-Zúñiga ◽  
María Rodriguez-Solis ◽  
Verónica Villalobos-Barquero ◽  
...  

2016 ◽  
Vol 33 ◽  
pp. S137
Author(s):  
Andrés Merino Restrepo ◽  
Angelina Hormaza Anaguano ◽  
Carolina Velásquez Quintero

BioResources ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. 3797-3807
Author(s):  
Magdah Ganash ◽  
Tarek M. Abdel Ghany ◽  
Mohamed A. Al Abboud ◽  
Mohamed M. Alawlaqi ◽  
Husam Qanash ◽  
...  

Lignocellulolytic white-rot fungi allow the bioconversion of agricultural wastes into value-added products that are used in a myriad of applications. The aim of this work was to use corn residues (Zea mays L.) to produce valuable products under solid-state fermentation (SSF) with Pleurotus ostreatus. White-rot fungus P. ostreatus was isolated from maize silage (MS) and thereafter it was inoculated on MS as substrate and compared with maize stover (MSt) and maize cobs (MC) to determine the best lignocellulosic substrate for the production of lignocellulolytic enzymes and extracellular protein. The MS gave the highest productivity of CMCase (368.2 U/mL), FPase (170.5 U/mL), laccase (11.4 U/mL), and MnPase (6.6 U/mL). This is compared to productivity on MSt of 222 U/mL, 50.2 U/mL, 4.55 U/mL, and 2.57 U/mL, respectively; and productivity on MC at the same incubation period as 150.5 U/mL, 48.2 U/mL, 3.58 U/mL, and 2.5 U/mL, respectively. The levels of enzyme production declined with increasing incubation period after 15 and 20 days using MS and MC, respectively, as substrates. Maximum liberated extracellular protein content (754 to 878 µg/mL) was recorded using MS, while a low amount (343 to 408 µg/mL) was liberated with using MSt and MC.


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