1533 Effect of anionic salts on rumen fermentation in a continuous culture system

2016 ◽  
Vol 94 (suppl_5) ◽  
pp. 744-744
Author(s):  
A. L. Kenny ◽  
J. L. Purdom ◽  
M. M. Masiero ◽  
J. P. Jarrett ◽  
T. J. Wistuba ◽  
...  
Keyword(s):  
Continuous Culture ◽  
Culture System ◽  
Rumen Fermentation ◽  
Continuous Culture System

scholarly journals Design and development of a continuous culture system for studying rumen fermentation

1989 ◽  
Vol 61 (5) ◽  
pp. 463-473
Author(s):  
Harri Miettinen ◽  
Jouko Setälä
Keyword(s):  
Continuous Culture ◽  
Culture System ◽  
Artificial Saliva ◽  
Present Report ◽  
Ammonia Concentration ◽  
Rumen Fermentation ◽  
Liquid Volume ◽  
Desktop Computer ◽  
Continuous Culture System

The present report describes an in vitro continuous culture system to simulate rumen fermentation. The complete assembly consists of six culture vessels (liquid volume 700 ml) fed twice daily with finely ground feed. The artificial saliva enters the vessel continuously, and the effluent leaves it continuously through the overflow port. The intermittent stirring of the fermentor content and the pH regulation are automatically controlled by a desktop computer. Two replicate experiments with ten fermentors given a diet of silage (50 %) and barley (50 %) were made in order to evaluate the system. The results indicate that the system reaches steady-state conditions within three to five days, ammonia concentration being an exeption. It takes for the ammonia concentration approximately 11—14 days to stabilize. The plateau values for the total volatile fatty acid (VFA) concentrations, the molar proportions of individual VFAs, and the ammonia concentrations were found to be within the accepted range in the rumen of animals given similar diets or in other artificial rumen systems. There was a tenfold decrease in the numbers of protozoa in the fermentors during the first four days of incubation. However, the average plateau value for the protozoa numbers (2.5 x 104/ml) is in the same range as in the dual flow continuous culture systems. The efficiency of the microbial N production was higher than that usually observed in vivo or in vitro (45 vs. 30 g/kg organic matter digested). The results indicate that this continuous culture system provides a reasonable estimate of rumen fermentation.

USE OF A CONTINUOUS CULTURE SYSTEM TO STUDY PHOSPHORUS REQUIREMENTS IN THE RUMEN

1984 ◽  
Vol 64 (5) ◽  
pp. 35-36 ◽  
Author(s):  
S. KOMISARCZUK ◽  
R. J. MERRY ◽  
A. B. McALLAN ◽  
R. H. SMITH ◽  
M. DURAND
Keyword(s):  
Fatty Acid ◽  
Continuous Culture ◽  
Key Words ◽  
Culture System ◽  
Volatile Fatty Acid ◽  
Phosphorus Deficiency ◽  
Rumen Fermentation ◽  
Rumen Microbes ◽  
Continuous Culture System ◽  
Phosphorus Requirements

It was found that when a stable rumen fermentation, established in a continuous culture apparatus, was subjected to severe phosphorus deficiency, there were marked reductions in protozoal numbers, volatile fatty acid and ATP concentrations, and increased pH and ammonia concentrations, indicating reduced fermentative and synthetic capacities of the rumen microbes. Key words: Continuous culture, rumen fermentation, phosphorus deficiency

Kinetics of biodegradation of Di-n-butyl phthalate in continuous culture system

Chemosphere ◽  
1998 ◽  
Vol 37 (2) ◽  
pp. 257-264 ◽  
Author(s):  
Jianlong Wang ◽  
Ping Liu ◽  
Hanchang Shi ◽  
Yi Qian
Keyword(s):  
Continuous Culture ◽  
Culture System ◽  
Continuous Culture System ◽  
Butyl Phthalate ◽  
Kinetics Of

Three-stage continuous culture system with a self-generated anaerobia to study the regionalized metabolism of the human gut microbiota

2014 ◽  
Vol 96 ◽  
pp. 111-118 ◽  
Author(s):  
David Feria-Gervasio ◽  
William Tottey ◽  
Nadia Gaci ◽  
Monique Alric ◽  
Jean-Michel Cardot ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Continuous Culture ◽  
Culture System ◽  
Human Gut ◽  
Human Gut Microbiota ◽  
Continuous Culture System

293 Nutritional evaluation of forage ephedra (Ephedra nevadensis) as an alternative forage using a dual-flow continuous culture system

2017 ◽  
Vol 95 (suppl_4) ◽  
pp. 145-145
Author(s):  
C. B. Sampaio ◽  
E. Marostegan de Paula ◽  
L. Galoro da Silva ◽  
V. Brandao ◽  
X. Dai ◽  
...  
Keyword(s):  
Continuous Culture ◽  
Culture System ◽  
Nutritional Evaluation ◽  
Continuous Culture System ◽  
Ephedra Nevadensis
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