New technologies in surgery and therapy of the cornea based on the use of riboflavin and subablative radiation modes of «Microscan Visum» excimer laser

2019 ◽  
Vol 30 (5) ◽  
pp. 287-291
Author(s):  
I.M. Kornilovskiy ◽  
◽  
S.K. Vartapetov ◽  
V.G. Movshev ◽  
D.V. Vedeneev ◽  
...  
2001 ◽  
Vol 36 (3) ◽  
pp. 123-124
Author(s):  
Jean-Marie R. Lance ◽  
Guy Régnier

Author(s):  
Warren Grundfest ◽  
Frank Litvack ◽  
Tsvi Goldenberg ◽  
James Forrester ◽  
James B. Laudenslager ◽  
...  

2017 ◽  
Vol 2017 (1) ◽  
pp. 000120-000125 ◽  
Author(s):  
Robert Gernhardt ◽  
Friedrich Müller ◽  
Markus Woehrmann ◽  
Habib Hichri ◽  
Karin Hauck ◽  
...  

Abstract Multi-chip integrated Fan-Out packages and high I/O CSPs demands for higher routing density on wafer level. Due to that, the classical mask aligner lithography and photosensitive thin-film polymers used for BEOL reach its limits and new technologies and materials are necessary to generate lines and space down to two μm. These multi-metal layers set also higher demands on the mechanical properties of the materials. This paper presents a new excimer laser dual damascene process for ultra-fine routing for BEOL. Various materials like low cure temperature polyimide, BCB and 15-μm thick dry-film ABF material are structured by using an excimer laser stepper with a reticle mask to realize feature size below four μm with a high throughput. Micro-vias with a diameter below five μm are realized with high aspect ratio, which overcome the photolithographic limitations of the common used photosensitive thin-film polymers. The laser structuring allows to use innovative dielectric materials for WLP with optimized mechanical and electrical parameters for example inorganic filled polymers like dry-film ABF materials, which do not have to be photosensitive. The ablations depth per laser pulse and the cross-section of the ablated structures in dependence of the ablation parameters was investigated. The depth of embedded lines was set by number of pulses aside of integrated micro-vias. The lines and micro-vias were metallized with copper by galvanic process and the following CMP step removes the copper outside the ablated structures. The CMP removes only the copper and the metal of the seed-layer, which has the functions of an adhesion and barrier layer, stays intact. The under-etching of the conventional wet etch seed layer removal is a major problem for the fine line structures realized by the Laser Dual Damascene process. Due to that, the removal of the seed layer (usually titan) was investigated and it could be shown, that this layer can be removed by the excimer laser system. The stepper like system allows a sub-micron alignment accuracy with no need of a capture pad of the embedded lines. Test structures have been designed and fabricated with lines and spaces below 10 μm to demonstrate the dense multi-layer routing capability where the excellent reliability can be proven by air to air thermal cycling (from −55°C up to 125°C), current leakage and electro migration test.


Author(s):  
Klaus-Ruediger Peters

Only recently it became possible to expand scanning electron microscopy to low vacuum and atmospheric pressure through the introduction of several new technologies. In principle, only the specimen is provided with a controlled gaseous environment while the optical microscope column is kept at high vacuum. In the specimen chamber, the gas can generate new interactions with i) the probe electrons, ii) the specimen surface, and iii) the specimen-specific signal electrons. The results of these interactions yield new information about specimen surfaces not accessible to conventional high vacuum SEM. Several microscope types are available differing from each other by the maximum available gas pressure and the types of signals which can be used for investigation of specimen properties.Electrical non-conductors can be easily imaged despite charge accumulations at and beneath their surface. At high gas pressures between 10-2 and 2 torr, gas molecules are ionized in the electrical field between the specimen surface and the surrounding microscope parts through signal electrons and, to a certain extent, probe electrons. The gas provides a stable ion flux for a surface charge equalization if sufficient gas ions are provided.


2019 ◽  
Vol 47 (5) ◽  
pp. 1247-1257 ◽  
Author(s):  
Mateusz Dyla ◽  
Sara Basse Hansen ◽  
Poul Nissen ◽  
Magnus Kjaergaard

Abstract P-type ATPases transport ions across biological membranes against concentration gradients and are essential for all cells. They use the energy from ATP hydrolysis to propel large intramolecular movements, which drive vectorial transport of ions. Tight coordination of the motions of the pump is required to couple the two spatially distant processes of ion binding and ATP hydrolysis. Here, we review our current understanding of the structural dynamics of P-type ATPases, focusing primarily on Ca2+ pumps. We integrate different types of information that report on structural dynamics, primarily time-resolved fluorescence experiments including single-molecule Förster resonance energy transfer and molecular dynamics simulations, and interpret them in the framework provided by the numerous crystal structures of sarco/endoplasmic reticulum Ca2+-ATPase. We discuss the challenges in characterizing the dynamics of membrane pumps, and the likely impact of new technologies on the field.


2020 ◽  
Vol 64 (2) ◽  
pp. 251-261
Author(s):  
Jessica E. Fellmeth ◽  
Kim S. McKim

Abstract While many of the proteins involved in the mitotic centromere and kinetochore are conserved in meiosis, they often gain a novel function due to the unique needs of homolog segregation during meiosis I (MI). CENP-C is a critical component of the centromere for kinetochore assembly in mitosis. Recent work, however, has highlighted the unique features of meiotic CENP-C. Centromere establishment and stability require CENP-C loading at the centromere for CENP-A function. Pre-meiotic loading of proteins necessary for homolog recombination as well as cohesion also rely on CENP-C, as do the main scaffolding components of the kinetochore. Much of this work relies on new technologies that enable in vivo analysis of meiosis like never before. Here, we strive to highlight the unique role of this highly conserved centromere protein that loads on to centromeres prior to M-phase onset, but continues to perform critical functions through chromosome segregation. CENP-C is not merely a structural link between the centromere and the kinetochore, but also a functional one joining the processes of early prophase homolog synapsis to late metaphase kinetochore assembly and signaling.


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