A simple reverse phase UPLC validated method for concurrent estimation of Emtricitabine, Darunavir, Tenofovir and Cobicistat in drug substances and drug product
A fast, precise, accurate and steady isocratic liquid chromatographic technique was created for the synchronous assurance of the Emtricitabine, Darunavir, Tenofovir and Cobicistat in bulk and in formulation. To optimize a column HSS C18 100 x 2.1 mm, 1.8mm, mobile phase including Buffer 0.01N KH2PO4(5.4pH): Acetonitrile pick in the proportion 60:40v/v was pumped through column at a flow rate of 0.3 ml/min at 260nm. The retention times of Emtricitabine, Darunavir, Tenofovir and Cobicistat were initiated to be 1.066 min, 1.727 min, 2.574 min and 2.977 min. % recovery was 99.75%, 100.05%, 100.42% and 100.59% for Emtricitabine, Darunavir, Tenofovir and Cobicistat respectively. LOD and LOQ values got from relapse formula of Emtricitabine, Darunavir, Tenofovir and Cobicistat and were 0.18, 0.54, 0.71,2.14, 0.01,0.05 0.44 and 1.32 correspondingly. Relapse equation of Emtricitabine is y = 13375x + 2577.4, for Darunavir it is y = 7196.3x + 2981.2, for Tenofovir it is y = 66663x + 1338.9 and y = 22723x + 6978.7 for Cobicistat.