Tissue Expression of the S100 Protein Family-related MRP8 Gene in Human Chorionic Villi by in situ Hybridization Techniques

Lymphoepithelioma-like carcinoma is a non-nasopharyngeal undifferentiated carcinoma with prominent lymphoid infiltration. Ten cases arising in the lung have been reported so far; seven cases were diagnosed in Orientals, with the Epstein-Barr virus (EBV) genome demonstrated in neoplastic cells by in situ hybridization; the remaining three cases affected Caucasian patients and showed no evidence of hybridized viral genome. The present study describes two additional cases of lymphoepithelioma-like carcinoma of the lung in Caucasians, with reference to the differential diagnosis versus other thoracomediastinal malignancies. The neoplastic nuclei, blastlike in appearance, together with the immunohistochemical profile of the neoplastic cells (positivity for cytokeratins, and negativity for CD antigens, S100 protein, placental alkaline phosphatase, and neuroendocrine markers) represent the basic pathologic features of a lymphoepithelioma-like carcinoma and allow its recognition even on small bioptic fragments, in which the typical biphasic, Regaud-like morphology might be inapparent. In accordance with the previously reported cases of pulmunary lymphoepithelioma-like carcinoma in Caucasian patients, the present study found no evidence of the Epstein-Barr virus genome in neoplastic cells with in situ hybridization.

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Localization of cells from the winter flounder gill expressing a skin type antifreeze protein gene

Canadian Journal of Zoology ◽

10.1139/z01-209 ◽

2002 ◽

Vol 80 (1) ◽

pp. 110-119 ◽

Cited By ~ 10

Author(s):

Harry M Murray ◽

Choy L Hew ◽

Ken R Kao ◽

Garth L Fletcher

Keyword(s):

In Situ Hybridization ◽

Antifreeze Protein ◽

Tissue Expression ◽

Cross Reactivity ◽

Winter Flounder ◽

Skin Type ◽

General Distribution ◽

Pavement Cells ◽

Peripheral Tissues

In situ hybridization on whole mounts and paraffin-sectioned winter flounder (Pleuronectes americanus) gill, using riboprobes specific to a skin type antifreeze protein (AFP) gene, showed a mRNA distribution associated with cells throughout the filament and the lamellae. Immunohistochemistry using antibodies for a skin-type AFP identified cells corresponding to those detected using in situ hybridization. Parallel experiments with antibodies for chloride-cell markers showed that these cells were not involved in antifreeze-protein expression. Similarly, goblet cells did not show cross-reactivity with the AFP antibodies. This general distribution suggested that pavement cells were likely involved. Reverse transcription polymerase chain reaction using gill cDNA templates and subsequent sequencing of the products confirmed the presence of skin type AFP transcripts in this tissue. Expression of a AFP in this area may act as a first line of defence against ice-crystal migration into peripheral tissues.

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Localization of mRNA for the β-subunit of placental hCG by in situ hybridization

Acta Endocrinologica ◽

10.1530/acta.0.1190069 ◽

1988 ◽

Vol 119 (1) ◽

pp. 69-74 ◽

Cited By ~ 5

Author(s):

Mariann Wide ◽

Håkan Persson ◽

Örjan Lundkvist ◽

Leif Wide

Keyword(s):

In Situ Hybridization ◽

Amino Acid ◽

Amino Acid Sequence ◽

Background Noise ◽

Placental Tissue ◽

Β Subunit ◽

Chorionic Villi ◽

Cellular Origin ◽

Β Hcg

Abstract. The cellular origin of placental hCG is not yet completely established. Depending on the method used, the syncytium, cytotrophoblast or both types of tissue have been claimed to synthesize hCG. In the present study in situ hybridization was used on sections of chorionic villi in order to detect expression of the gene for the β-subunit of hCG (β-hCG). Placental tissue was obtained from the 8th to the 11th weeks of pregnancy, when the concentration of hCG is high. A cDNA clone, encoding the entire amino acid sequence of β-hCG, was used as a probe. Hybrids of β-hCG cDNA/mRNA were found only over the syncytiotrophoblast. Background noise was extremely low and no signals above that were detected in the cytotrophoblast cells. It is concluded that at this stage of pregnancy the gene for β-hCG is activated in the syncytial parts of chorionic villi and not in the cytotrophoblast.

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Specific tissue expression and cellular localization of human apolipoprotein M as determined by in situ hybridization

Acta Histochemica ◽

10.1078/0065-1281-00687 ◽

2003 ◽

Vol 105 (1) ◽

pp. 67-72 ◽

Cited By ~ 58

Author(s):

Xiao-Ying Zhang ◽

Xuan Dong ◽

L.u. Zheng ◽

Guang-Hua Luo ◽

Yuan-Hua Liu ◽

...

Keyword(s):

In Situ Hybridization ◽

Cellular Localization ◽

Tissue Expression ◽

Apolipoprotein M ◽

Human Apolipoprotein ◽

Specific Tissue

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Mouse Myosin X: Molecular Architecture and Tissue Expression as Revealed by Northern Blot and in Situ Hybridization Analyses

Biochemical and Biophysical Research Communications ◽

10.1006/bbrc.2000.2669 ◽

2000 ◽

Vol 271 (2) ◽

pp. 526-533 ◽

Cited By ~ 18

Author(s):

Satoshi Yonezawa ◽

Atsushi Kimura ◽

Seizo Koshiba ◽

Shigeo Masaki ◽

Takao Ono ◽

...

Keyword(s):

In Situ Hybridization ◽

Northern Blot ◽

Tissue Expression ◽

Molecular Architecture ◽

Myosin X

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Guinea Pig Histamine H1 Receptor. I. Gene Cloning, Characterization, and Tissue Expression Revealed by In Situ Hybridization

Journal of Neurochemistry ◽

10.1046/j.1471-4159.1994.62020507.x ◽

2002 ◽

Vol 62 (2) ◽

pp. 507-518 ◽

Cited By ~ 45

Author(s):

E. Traiffort ◽

R. Leurs ◽

J. M. Arrang ◽

J. Tardivel-Lacombe ◽

J. Diaz ◽

...

Keyword(s):

In Situ Hybridization ◽

Guinea Pig ◽

Gene Cloning ◽

Tissue Expression ◽

H1 Receptor ◽

Histamine H1 Receptor ◽

I Gene

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DNA sequence mapping in interphase and metaphase chromosomes by fluorescence in situ hybridization

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100122885 ◽

1992 ◽

Vol 50 (1) ◽

pp. 496-497

Author(s):

Barbara Trask ◽

Susan Allen ◽

Anne Bergmann ◽

Mari Christensen ◽

Anne Fertitta ◽

...

Keyword(s):

In Situ Hybridization ◽

Dna Sequence ◽

Dna Sequences ◽

Dual Band ◽

Nick Translation ◽

Metaphase Chromosomes ◽

Band Pass ◽

Texas Red ◽

Fluorescent Spot

Using fluorescence in situ hybridization (FISH), the positions of DNA sequences can be discretely marked with a fluorescent spot. The efficiency of marking DNA sequences of the size cloned in cosmids is 90-95%, and the fluorescent spots produced after FISH are ≈0.3 μm in diameter. Sites of two sequences can be distinguished using two-color FISH. Different reporter molecules, such as biotin or digoxigenin, are incorporated into DNA sequence probes by nick translation. These reporter molecules are labeled after hybridization with different fluorochromes, e.g., FITC and Texas Red. The development of dual band pass filters (Chromatechnology) allows these fluorochromes to be photographed simultaneously without registration shift.

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Ultrastructural analysis of the spatial distribution of MRNA

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100123167 ◽

1992 ◽

Vol 50 (1) ◽

pp. 552-553

Author(s):

Gary Bassell ◽

Robert H. Singer

Keyword(s):

Electron Microscopy ◽

Spatial Distribution ◽

In Situ Hybridization ◽

High Resolution ◽

Nucleic Acids ◽

Colloidal Gold ◽

Dna Probe ◽

Nucleotide Analogs ◽

Gold Particles

We have been investigating the spatial distribution of nucleic acids intracellularly using in situ hybridization. The use of non-isotopic nucleotide analogs incorporated into the DNA probe allows the detection of the probe at its site of hybridization within the cell. This approach therefore is compatible with the high resolution available by electron microscopy. Biotinated or digoxigenated probe can be detected by antibodies conjugated to colloidal gold. Because mRNA serves as a template for the probe fragments, the colloidal gold particles are detected as arrays which allow it to be unequivocally distinguished from background.

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