scholarly journals Comparison of chondro-plastic properties of the chondrogide and chondro-teck collagen membranes did not reveal reparative differences in the rat model of full thickness defect of articular cartilage

2020 ◽  
pp. 102-109
Author(s):  
Е.В. Афанасьевская ◽  
Е.В. Медведева ◽  
Б.М. Газимиева ◽  
А.Д. Куренкова ◽  
О.В. Кытько ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Hyaline Cartilage ◽  
Articular Surface ◽  
Fibrous Tissue ◽  
Cartilage Defects ◽  
Collagen Membrane ◽  
Full Thickness ◽  
Macroscopic Appearance ◽  
Defect Area ◽  
Collagen Membranes

Введение. Для заполнения костно-хрящевых дефектов широкое применение получили коллагенсодержащие импланты. Наибольшей популярностью пользуется коллагеновая мембрана Chondro-Gide® (Швейцария) Предполагается, что после пересадки она запускает естественный механизм хондрогенеза. Однако некоторые клинические исследования показывают, что отдаленные результаты не всегда положительны. В ПМГМУ им. И.М. Сеченова изготовлена коллагеновая мембрана Хондротек (рабочее название), которая в эксперименте показала хорошие результаты при выполнении реконструкции дефектов гиалинового хряща. Цель исследования - сопоставление хондропластических свойств двух коллагеновых мембран по восстановлению полнослойного дефекта суставного хряща у крыс в эксперименте и оценка возможности импортозамещения. Методика. Выполнено 3 серии экспериментов на коленных суставах 18 крыс линии Вистар: контрольная группа - без восстановления дефекта и две опытные группы с имплантацией одной из мембран в область дефекта. Полнослойный дефект хряща воспроизводили в межмыщелковой ямке коленного сустава фрезой диаметром 2,5 мм до появления кровяной росы на дне дефекта. Импланты из мембран соответствующего размера помещали в дефект и прикрывали надколенником. Дополнительной фиксации не требовалось. Срок наблюдения 2 и 4 мес. Процессы репаративной регенерации оценивали визуально с применением шкалы ICRS и с помощью гистологических методов исследования. Результаты. Применение коллагеновых мембран ускоряло восстановление тканей в области дефекта. Хондропластические свойства импортной и отечественной мембран были аналогичными. Признаков восстановления гиалинового хряща не было обнаружено ни в контрольной, ни в одной из опытных групп. Утраченные ткани замещались фиброзным хрящом. Заключение. Обе исследованные коллагеновые мембраны (отечественная Хондротек и импортная Chondro-Gide®) могут быть использованы для восстановления целостности поврежденного хряща и восстановления конгруэнтности суставных поверхностей. Процесс восстановления происходил за счет формирования фиброзного хряща, регенерация гиалинового хряща не наблюдалась ни в одной из групп. Хондропластические свойства импортной и отечественной мембран оказались аналогичными, что доказывает возможность рекомендовать применение последней в клинической практике. Полученные данные позволяют рекомендовать мембраны Хондротек для импортозамещения. Background. Collagen-based membranes and scaffolds are widely used for implantation into various bone and cartilage defects. The Chondro-Gide® (Switzerland) collagen membrane is among membranes widely used for defects of articular cartilage in orthopedic practice of the Russian Federation. This membrane is considered to trigger a natural chondrogenesis mechanism. However, some clinical studies have shown that remote results are not always beneficial. A collagen-based membrane (working name, Chondroteck), which showed properties compatible to those of Chondro-Gide® in in vitro experiments, was recently developed at the Sechenov University (1st MSMU). Aim. To compare chondrogenic properties of these two membranes in vivo and to explore a possibility of using the Chondroteck membrane for restoration of full-thickness defects in articular cartilages. Methods. The full-thickness defects were created in the intercondylar fossa of 18 adult Wistar rats by drilling a 2.5 mm diameter hole into the subchondral bone (until small blood drops appeared on the bottom of the defect). Then rats were divided into three groups, control (no membrane added) and two experimental groups, one group with Chondro-Gide® and another group with the Chondroteck membrane implanted into the defect. Membranes were cut to the appropriate size, placed into the defect and covered with the patella. No additional fixation of membranes was required. Cartilage repair was assessed using the ICRS scoring system on histological sections stained with hematoxylin/eosin or toluidine blue. Results. Both collagen membranes improved the curvature of the articular surface and macroscopic appearance of the cartilage and protected the tissues surrounding the defect area as compared to the control. However, no formation or restoration of hyaline cartilage was detected in any group. Instead, fibrous tissue was formed at the defect area, and this fibrous tissue was similar in both groups with membranes. Conclusions. Both Chondro-Gide® and Chondroteck membranes can be utilized for restoration of cartilage surface. Formation of hyaline cartilage does not occur with either membrane. Thus, chondroplastic properties of both membranes are comparable, which allows to recommend the locally made Chondroteck membrane for further testing.

scholarly journals Implantation of Various Cell-Free Matrixes Does Not Contribute to the Restoration of Hyaline Cartilage within Full-Thickness Focal Defects

2021 ◽  
Vol 23 (1) ◽  
pp. 292
Author(s):  
Shabnam I. Ibragimova ◽  
Ekaterina V. Medvedeva ◽  
Irina A. Romanova ◽  
Leonid P. Istranov ◽  
Elena V. Istranova ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Cellular Therapy ◽  
Hyaline Cartilage ◽  
Articular Surface ◽  
Cartilage Degeneration ◽  
Collagen Membrane ◽  
Full Thickness ◽  
Joint Tissue ◽  
Tissue Reconstruction ◽  
Articular Cartilage Degeneration

Articular cartilage is a highly organized tissue that has a limited ability to heal. Tissue engineering is actively exploited for joint tissue reconstruction in numerous cases of articular cartilage degeneration associated with trauma, arthrosis, rheumatoid arthritis, and osteoarthritis. However, the optimal scaffolds for cartilage repair are not yet identified. Here we have directly compared five various scaffolds, namely collagen-I membrane, collagen-II membrane, decellularized cartilage, a cellulose-based implant, and commercially available Chondro-Gide® (Geistlich Pharma AG, Wolhusen, Switzerland) collagen membrane. The scaffolds were implanted in osteochondral full-thickness defects, formed on adult Wistar rats using a hand-held cutter with a diameter of 2.0 mm and a depth of up to the subchondral bone. The congruence of the articular surface was almost fully restored by decellularized cartilage and collagen type II-based scaffold. The most vivid restoration was observed 4 months after the implantation. The formation of hyaline cartilage was not detected in any of the groups. Despite cellular infiltration into scaffolds being observed in each group except cellulose, neither chondrocytes nor chondro-progenitors were detected. We concluded that for restoration of hyaline cartilage, scaffolds have to be combined either with cellular therapy or morphogens promoting chondrogenic differentiation.

scholarly journals Experimental assessment of biological potential of collagen membranes in Reconstruction of full-thickness hyaline cartilage defects

2021 ◽  
Author(s):  
GD Lazishvili ◽  
KA Egiazaryan ◽  
DV Nikishin ◽  
AA Voroncov ◽  
DV Klinov
Keyword(s):  
Cartilage Defect ◽  
Hind Limb ◽  
Hyaline Cartilage ◽  
Cartilage Tissue ◽  
Cartilage Defects ◽  
Collagen Membrane ◽  
Full Thickness ◽  
Post Surgery ◽  
Biological Potential ◽  
Collagen Membranes

Investigation of the efficacy of collagen membranes used in the full-thickness hyaline cartilage defect surgery is extremely urgent from the point of view of everyday healthcare. However, there is no information about the collagen membrane transformation timeframe, patterns and type of tissue the membrane transforms into, nor on the quality of the newly formed cartilage, which hinders the use of collagen membranes in clinical practice. This study aimed to investigate the biological potential of collagen membranes and their capacity to transform into cartilage tissue. The study involved four pigs as subjects. We induced a full-thickness cartilage defect on their right hind limb joint and implanted an Ortokeep collagen membrane to remedy it. Two full-thickness cartilage defects were induced on the left hind limb joints of the animals, one was treated with an implanted Chondro-Gide collagen membrane, the other remained without a membrane. The animals were withdrawn from the experiment at 2, 3, 4, 6 months after the operation. This report contains results of the macroscopic and microscopic analyses revealing the character of cartilage tissue regeneration at various timepoints post-surgery. The collagen membranes proved to have a high biological potential and a capacity to transform into cartilage tissue. The cartilages were identifiable from the 3rd month of the study. Their thickness was growing significantly (p < 0.05) up to the 4th month post-surgery, gaining 18.7% in group 1 and 12.8% in group 2; afterwards, the formed tissue "matured". We have shown that the AMIC technique allows significant (p < 0.05) reduction of the bone tissue destruction area.

The Use of Particulated Juvenile Allograft Cartilage for the Repair of Porcine Articular Cartilage Defects

2019 ◽  
Vol 47 (10) ◽  
pp. 2308-2315 ◽  
Author(s):  
Yunong Ao ◽  
Zhong Li ◽  
Qi You ◽  
Chengchang Zhang ◽  
Liu Yang ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Statistical Difference ◽  
Fibrous Tissue ◽  
Cartilage Defects ◽  
Control Group ◽  
General View ◽  
Full Thickness ◽  
Femoral Trochlea ◽  
Articular Cartilage Defects ◽  
Experimental Group

Background: The repair of porcine articular cartilage defects by using particulated juvenile allograft cartilage (PJAC) has demonstrated good short-term clinical efficacy, but the repair process and mechanism have not been fully elucidated. Purpose: To study the efficacy of PJAC in repairing full-thickness cartilage defects and to provide an experimental basis for its clinical application. Study Design: Controlled laboratory study. Methods: Thirty Guizhou minipigs were randomly divided into an experimental group and control group. An 8-mm cylindrical full-thickness cartilage defect was created in the femoral trochlea of either knee in all minipigs. The experimental group received the PJAC transplantation (PJAC group; n = 15) and the control group received autologous cartilage chips (ACC group; n = 15). Five minipigs were euthanized at 1, 3, and 6 months in each group to obtain samples, which were evaluated by general view of the knee joint and histomorphometry of the chondral defect area (hematoxylin and eosin, safranin O). International Cartilage Repair Society (ICRS) II semiquantitative evaluation and collagen type II staining immunohistochemistry were also performed. Results: All 30 Guizhou minipigs were followed; there was no infection or incision healing disorder after the operation. At 1 month postoperatively, more hyaline cartilage was found in the ACC group (29.4%) compared with the PJAC group (20.1%) ( P < .05); there was no statistical difference between the 2 groups at 3 and 6 months after operation. The fibrocartilage content in the ACC group was significantly more than that in the PJAC group at 1 and 3 months postoperatively (27.4% vs 18.2% and 49.9% vs 41.1%, respectively; P < .05); significant differences disappeared at 6 months postoperatively. The PJAC group produced more fibrous tissue than the ACC group at 1 and 3 months postoperatively (60.1% vs 40.6% and 38.8% vs 24.4%, respectively; P < .05) but showed no statistical difference at 6 months postoperatively. Regarding the ICRS II scores, those of the ACC group were significantly better than the scores of the PJAC group in some subclasses at 3 and 6 months postoperatively. The positive rates of immunohistochemical staining in the ACC group were higher at 1 and 3 months postoperatively than those in the PJAC group (54.2% vs 37.8% and 46.4% vs 34.4%, respectively; P < .05). The difference was not statistically significant between the 2 groups at 6 months postoperatively. Conclusion: Both PJAC and ACC can produce a good repair effect on cartilage defects. At 1 and 3 months postoperatively, ACC resulted in better outcomes than PJAC, but there was no statistical difference in the repair effect between the 2 techniques at 6 months postoperatively. Clinical Relevance: Based on this animal experiment, further clinical studies are needed to investigate PJAC as a possible alternative first-line treatment for cartilage defects.

scholarly journals Autologous Costal Cartilage Graft—A New Method to Treat Articular Cartilage Defects: Case Report and Note of Surgical Technique

Joints ◽  
2018 ◽  
Vol 06 (04) ◽  
pp. 246-250
Author(s):  
Antonio Gigante ◽  
Marco Cianforlini ◽  
Luca Farinelli ◽  
Riccardo Girotto ◽  
Alberto Aquili
Keyword(s):  
Articular Cartilage ◽  
Femoral Condyle ◽  
Costal Cartilage ◽  
Donor Site ◽  
Standard Technique ◽  
Cartilage Defects ◽  
Cartilage Graft ◽  
Full Thickness ◽  
Articular Cartilage Defects ◽  
Costal Cartilage Graft

AbstractFull-thickness articular cartilage defects do not heal spontaneously. Several techniques have been developed to address this issue, but none resulted in the restitutio ad integrum of the articular cartilage. The most frequent sites of chondral lesion in the knee are medial femoral condyle and patella. The patellofemoral lesions are characterized by outcomes that are generally worse than those of tibiofemoral ones. To date, it has been well recognized the chondrogenic potential of rib perichondrium, and costal cartilage grafts have been extensively used in reconstructive surgery. Considering the need to find a gold standard technique to restore articular defect, we developed and here described a new technique to repair cartilage lesions of the knee using autologous costal cartilage graft with its perichondrium. This innovative surgical approach can be used to treat full thickness articular defects using autologous hyaline cartilage, making it possible to cover wide defects. This one step technique is low invasive, not technically demanding with minimal donor site morbidity and it has low costs. The long-term clinical efficacy of the method remains to be evaluated.

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