scholarly journals Blood corticosterone in rats with different susceptibility to audiogenic epilepsy

2018 ◽  
pp. 64-67
Author(s):  
И.Б. Федотова ◽  
А.В. Поликарпова ◽  
О.В. Перепелкина ◽  
Г.М. Николаев ◽  
О.В. Смирнова ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Stress Reaction ◽  
Blood Levels ◽  
Practical Issue ◽  
Audiogenic Epilepsy ◽  
Sound Exposure ◽  
Selection History ◽  
History Of ◽  
Rat Strain ◽  
Seizure Episode

Актуальность. Селектированная на проявление судорог в ответ на сильный звук (аудиогенную эпилепсию, АЭ) линия крыс Крушинского-Молодкиной (КМ) - модель судорожных состояний человека, c быстрым развитием судорожного припадка в ответ на включение звука (100-120 дБ). Однако у крыс с АЭ изменений уровня кортикостерона (КС) в крови в связи с судорогами не определяли, хотя анализ связи АЭ и стресс-реакции - важная практическая задача. Методы. Уровень КС в образцах сыворотки крови определяли с помощью набора у крыс линии КМ, а также у крыс линии «0», селектированных из гибридной популяции КМ х Вистар на отсутствие АЭ, и у крыс популяции Вистар. Результаты. У всех крыс КМ был зарегистрирован аудиогенный припадок, тогда как у крыс линии «0» и Вистар судорог не было. Через 30 мин после действия звука (и судорог) у крыс КМ повышеается уровень КС, тогда как сразу после судорог (через 1-3 мин) данная реакция не обнаруживается. У крыс линии «0» обнаружен достоверно более высокий фоновый уровень КС, по сравнению с КМ и Вистар. Уровень КС у них, как и у крыс Вистар, после действия звука не изменялся. Заключение. Подъем уровня КС в крови после действия звука наблюдали только у крыс КМ через 30 мин после припадка АЭ. Крысы линии «0», у которых нет судорог в ответ на звук, обнаружили более высокий, чем у КМ и Вистар уровень КС в фоне. Aims. The Krushinsky-Molodkina (KM) rat strain, which was selected for a seizure response to a sound (audiogenic epilepsy, AE), is known as a model of human seizure states. Although the association of blood CS with stress reaction is an important practical issue, blood levels of corticosterone (CS) have never been analyzed in rats with AE in relation with seizures. Methods. Serum concentration of CS was measured using IBL International Corticosterone ELISA kits in KM rats, rats of the «0» strain, which was selected (based on F2 KM х Wistar hybrids) for the lack of seizures in response to a sound, and Wistar rats. Results. All KM rats developed AE seizures in response to a sound while no seizures were observed in rats of the «0» strain and Wistar rats. The increase in blood CS was observed only in KM rats at 30 min after AE seizure. At background, the level of CS was significantly higher in rats of the «0» strain, which did not develop seizures in response to a sound, than in KM and Wistar rats. CS levels remained unchanged in both «0» and Wistar rats after the sound exposure. Conclusions. The increase in blood CS occurred in KM rats at 30 min after the seizure episode. At background, the CS level was higher in rats of the «0» strain, which did not respond with seizures to a sound, than in Wistar and KM rats. Therefore, the blood level of CS depends in a complicated way on both AE and the selection history of the «0» rat strain.

Association between blood levels of PCDDs/PCDFs/dioxin-like PCBs and history of allergic and other diseases in the Japanese population

2012 ◽  
Vol 86 (8) ◽  
pp. 849-859 ◽  
Author(s):  
Mariko Nakamoto ◽  
Kokichi Arisawa ◽  
Hirokazu Uemura ◽  
Sakurako Katsuura ◽  
Hidenobu Takami ◽  
...  
Keyword(s):  
Japanese Population ◽  
Blood Levels ◽  
History Of

scholarly journals Gene Banks as Reservoirs to Detect Recent Selection: The Example of the Asturiana de los Valles Bovine Breed

2021 ◽  
Vol 12 ◽  
Author(s):  
Simon Boitard ◽  
Cyriel Paris ◽  
Natalia Sevane ◽  
Bertrand Servin ◽  
Kenza Bazi-Kabbaj ◽  
...  
Keyword(s):  
Production Systems ◽  
Cattle Breed ◽  
Time Data ◽  
Gene Banks ◽  
Animal Genetic Resources ◽  
Selection History ◽  
History Of ◽  
Rich Information ◽  
Different Populations ◽  
Candidate Regions

Gene banks, framed within the efforts for conserving animal genetic resources to ensure the adaptability of livestock production systems to population growth, income, and climate change challenges, have emerged as invaluable resources for biodiversity and scientific research. Allele frequency trajectories over the few last generations contain rich information about the selection history of populations, which cannot be obtained from classical selection scan approaches based on present time data only. Here we apply a new statistical approach taking advantage of genomic time series and a state of the art statistic (nSL) based on present time data to disentangle both old and recent signatures of selection in the Asturiana de los Valles cattle breed. This local Spanish originally multipurpose breed native to Asturias has been selected for beef production over the last few generations. With the use of SNP chip and whole-genome sequencing (WGS) data, we detect candidate regions under selection reflecting the effort of breeders to produce economically valuable beef individuals, e.g., by improving carcass and meat traits with genes such as MSTN, FLRT2, CRABP2, ZNF215, RBPMS2, OAZ2, or ZNF609, while maintaining the ability to thrive under a semi-intensive production system, with the selection of immune (GIMAP7, GIMAP4, GIMAP8, and TICAM1) or olfactory receptor (OR2D2, OR2D3, OR10A4, and 0R6A2) genes. This kind of information will allow us to take advantage of the invaluable resources provided by gene bank collections from local less competitive breeds, enabling the livestock industry to exploit the different mechanisms fine-tuned by natural and human-driven selection on different populations to improve productivity.

Case of eustachian valve endocarditis and the importance of synergistic antibiotic therapy

2021 ◽  
Vol 14 (6) ◽  
pp. e242553
Author(s):  
Dilpat Kumar ◽  
James Boyer ◽  
Warsha Fnu ◽  
Harry Boamah
Keyword(s):  
End Stage Renal Disease ◽  
Altered Mental Status ◽  
Blood Levels ◽  
Chronic Haemodialysis ◽  
Dialysis Catheter ◽  
Left Forearm ◽  
Eustachian Valve ◽  
History Of ◽  
Stage Renal Disease ◽  
End Stage

A 46-year-old woman with a history of end-stage renal disease on chronic haemodialysis presented with 1 week of fever, chills, altered mental status and hand pain. She was febrile and ill-appearing on presentation with a pulse rate of 102 beats per minute. She had a tunnelled dialysis catheter in her right neck. Hand examination demonstrated a swollen, erythematous and tender wrist. Cardiovascular examination demonstrated no murmurs. CT of the hand showed abscesses involving the left forearm. Blood and abscess cultures grew methicillin-resistant Staphylococcus aureus (MRSA). Transesophageal echocardiography (TEE) showed a 1.0×1.0 cm mobile vegetation involving the eustachian valve (EV), confirming EV endocarditis. She remained bacteraemic for 18 days despite being on vancomycin with appropriate blood levels. Vancomycin was switched to daptomycin and ceftaroline, which cleared her cultures. Repeat TEE showed improved vegetation size. Our case highlights the rarity and management of EV endocarditis and the importance of synergy for treatment of persistent MRSA bacteraemia.

scholarly journals Natural history of experimental arthritis induced by Paracoccidioides brasiliensis in wistar rats

2019 ◽  
Vol 52 ◽  
Author(s):  
Eduardo Alexandre Loth ◽  
Vanessa Cecatto ◽  
Rodrigo Daniel Genske ◽  
Maricília Silva Costa ◽  
Rinaldo Ferreira Gandra ◽  
...  
Keyword(s):  
Natural History ◽  
Wistar Rats ◽  
Paracoccidioides Brasiliensis ◽  
Experimental Arthritis ◽  
History Of

Effects of aldosterone on potassium recycling in the kidney of adrenalectomized rats

1985 ◽  
Vol 248 (2) ◽  
pp. F219-F227 ◽  
Author(s):  
E. Higashihara ◽  
J. P. Kokko
Keyword(s):  
Wistar Rats ◽  
Collecting Duct ◽  
Distal Tubule ◽  
Feedback System ◽  
Blood Levels ◽  
Segmental Analysis ◽  
Urinary Potassium ◽  
Papillary Collecting Duct ◽  
Potassium Secretion ◽  
Potassium Recycling

Recent studies have suggested that potassium, like urea, undergoes medullary recycling. The present cortical and papillary micropuncture studies were designed to confirm the existence of medullary potassium recycling and to determine whether acute infusions of aldosterone affected this phenomenon. Thus, nephron segmental analysis of potassium and sodium transport was conducted in adrenalectomized Munich-Wistar rats and similarly prepared rats that received aldosterone acutely to achieve physiological blood levels. The clearance results demonstrated that aldosterone has an acute antinatriuretic and a kaliuretic effect, whereas the micropuncture studies demonstrated that 1) aldosterone increases potassium secretion between early and late distal tubule punctures; 2) aldosterone causes an increase in delivery of potassium to the papillary collecting duct; 3) aldosterone does not increase potassium secretion across the papillary collecting duct; and 4) aldosterone significantly increases medullary potassium recycling as evidenced by increased quantities of potassium present at the bend of the loop of Henle in response to aldosterone infusions. Thus, the studies confirm the existence of potassium recycling and suggest that this phenomenon is a feedback system that, in part, regulates urinary potassium excretion.

A comparison of serotonin (5-HT) blood levels and activity of 5-HT2 antagonists in adjuvant arthritic Lewis and Wistar rats

1993 ◽  
Vol 38 (S2) ◽  
pp. C98-C101 ◽  
Author(s):  
M. Pertsch ◽  
K. Krause ◽  
R. Hirschelmann
Keyword(s):  
Wistar Rats ◽  
Blood Levels

An Influence of Player’s Ballot-Weight Changing in Consultation Algorithm

2013 ◽  
Vol 17 (5) ◽  
pp. 707-714
Author(s):  
Keisuke Touma ◽  
◽  
Satoshi Endo ◽  
Naruaki Toma ◽  
Yuhei Akamine ◽  
...  
Keyword(s):  
Experimental Results ◽  
Evaluation Function ◽  
Selection History ◽  
History Of

The validity of the consultation algorithm is shown in computer shogi. The consultation algorithm determines a move by votes of computer shogi players. In this research, we discuss a method that changes player vote weights during a game. To change player vote weights, we use the selection history of the move and the evaluation function value. Experimental results suggests the validity of our proposed method.

scholarly journals Impact des extraits éthyliques des feuilles de Hexalobus Monopétalus sur lemétabolisme protéiques des rats Wistar

2018 ◽  
Vol 6 (01) ◽  
Author(s):  
Akotegnon Rodrigue ◽  
Michodjehoun Loetitia ◽  
Behanzin Justin ◽  
Houeze Elisabeth ◽  
Sezan Alphonse
Keyword(s):  
Uric Acid ◽  
Total Protein ◽  
Wistar Rats ◽  
Polyphenolic Compounds ◽  
Blood Levels ◽  
Phytochemical Screening ◽  
Duration Of Treatment ◽  
Anthracene Derivatives ◽  
The Impact

The aim of this study was to evaluate the impact of the ethyl extract of dried leaves of Hexalobus monopetalus on protein metabolism in Wistar rats. After a phytochemical screening of extracts, total protein, urea, uric acid and creatinine were measured spectrophotometrically at six (06) sets of three (03) Wistar rats each. Batches 2 to 6 were given with orally administered respectively the daily doses of: 0.25 mg / kg, 0.5mg / kg, 1mg / kg, 1.5mg / kg and 2.5mg kg body weight of the extracts (for 14 days). The lot1 witness received distilled water instead of the extract. The results of the phytochemical screening revealed the presence polyphenolic compounds (gallic tannins, catechin tannin or condensed, anthocyanins, and leuco anthocyanins), flavonoids, mucilages reducing compounds, alkaloids, certain anthracene derivatives ( free anthracene, the O-glycosides), Steroids, and quinone derivatives. It was observed a significant increase (p ˂ 0.05) blood levels of total protein and uric acid and a significant (p ˂ 0.05) blood urea and creatinine levels in treated plots to extracts. These observed variations did not depend on the administered dose of the extracts but were depending on the duration of treatment; they were outstanding from the 7th day of treatment. The long term use of ethyl extract of dried leaves of Hexalobus monopetalus could compromise the liver function and the kidneys and may inhibit apoptosis in Wistar rats.

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