The positive modulator of AMPA receptors, S 47445, improved behavior, cognitive, neurochemical and synaptic proteins modifications induced by prenatal restraint stress

2016 ◽  
Author(s):  
Sylvie Bretin
Keyword(s):  
Ampa Receptors ◽  
Restraint Stress ◽  
Synaptic Proteins ◽  
Positive Modulator ◽  
Prenatal Restraint Stress

scholarly journals The STEP61 interactome reveals subunit-specific AMPA receptor binding and synaptic regulation

2019 ◽  
Vol 116 (16) ◽  
pp. 8028-8037 ◽  
Author(s):  
Sehoon Won ◽  
Salvatore Incontro ◽  
Yan Li ◽  
Roger A. Nicoll ◽  
Katherine W. Roche
Keyword(s):  
Ampa Receptors ◽  
Protein Tyrosine Phosphatase ◽  
Protein Phosphatase ◽  
Tyrosine Phosphatase ◽  
Hippocampal Slices ◽  
Specific Protein ◽  
Synaptic Proteins ◽  
Synaptic Currents ◽  
Synaptic Regulation ◽  
Binding Partners

Striatal-enriched protein tyrosine phosphatase (STEP) is a brain-specific protein phosphatase that regulates a variety of synaptic proteins, including NMDA receptors (NAMDRs). To better understand STEP’s effect on other receptors, we used mass spectrometry to identify the STEP61 interactome. We identified a number of known interactors, but also ones including the GluA2 subunit of AMPA receptors (AMPARs). We show that STEP61 binds to the C termini of GluA2 and GluA3 as well as endogenous AMPARs in hippocampus. The synaptic expression of GluA2 and GluA3 is increased in STEP-KO mouse brain, and STEP knockdown in hippocampal slices increases AMPAR-mediated synaptic currents. Interestingly, STEP61 overexpression reduces the synaptic expression and synaptic currents of both AMPARs and NMDARs. Furthermore, STEP61 regulation of synaptic AMPARs is mediated by lysosomal degradation. Thus, we report a comprehensive list of STEP61 binding partners, including AMPARs, and reveal a central role for STEP61 in differentially organizing synaptic AMPARs and NMDARs.

scholarly journals GluR2 protein-protein interactions and the regulation of AMPA receptors during synaptic plasticity

2003 ◽  
Vol 358 (1432) ◽  
pp. 715-720 ◽  
Author(s):  
Fabrice Duprat ◽  
Michael Daw ◽  
Wonil Lim ◽  
Graham Collingridge ◽  
John Isaac
Keyword(s):  
Synaptic Plasticity ◽  
Ampa Receptor ◽  
Protein Interactions ◽  
Ampa Receptors ◽  
Long Term Potentiation ◽  
Synaptic Proteins ◽  
Mammalian Brain ◽  
Protein Protein Interactions ◽  
Term Potentiation

AMPA-type glutamate receptors mediate most fast excitatory synaptic transmissions in the mammalian brain. They are critically involved in the expression of long-term potentiation and long-term depression, forms of synaptic plasticity that are thought to underlie learning and memory. A number of synaptic proteins have been identified that interact with the intracellular C-termini of AMPA receptor subunits. Here, we review recent studies and present new experimental data on the roles of these interacting proteins in regulating the AMPA receptor function during basal synaptic transmission and plasticity.

Impact of an acute exposure to ethanol on the oxidative stress status in the hippocampus of prenatal restraint stress adolescent male rats

2008 ◽  
Vol 1191 ◽  
pp. 55-62 ◽  
Author(s):  
Mihaela Enache ◽  
Vincent Van Waes ◽  
Elisabeth Vinner ◽  
Michel Lhermitte ◽  
Stefania Maccari ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Restraint Stress ◽  
Acute Exposure ◽  
Male Rats ◽  
Adolescent Male ◽  
Oxidative Stress Status ◽  
Prenatal Restraint Stress

Prenatal restraint stress decreases the expression of alpha-7 nicotinic receptor in the brain of adult rat offspring

Stress ◽  
2015 ◽  
Vol 18 (4) ◽  
pp. 435-445 ◽  
Author(s):  
Carlos J. Baier ◽  
María E. Pallarés ◽  
Ezequiela Adrover ◽  
Melisa C. Monteleone ◽  
Marcela A. Brocco ◽  
...  
Keyword(s):  
Nicotinic Receptor ◽  
Restraint Stress ◽  
Adult Rat ◽  
Rat Offspring ◽  
Alpha 7 ◽  
The Brain ◽  
Prenatal Restraint Stress

scholarly journals Loss of EPAC2 results in altered synaptic composition of dendritic spines and excitatory and inhibitory balance

2019 ◽  
Author(s):  
Kelly A Jones ◽  
Michiko Sumiya ◽  
Kevin M Woolfrey ◽  
Deepak P Srivastava ◽  
Peter Penzes
Keyword(s):  
Ampa Receptors ◽  
Cortical Neurons ◽  
Glutamate Transporter ◽  
Autism Spectrum ◽  
Small Gtpase ◽  
Anterior Cingulate ◽  
Synaptic Proteins ◽  
Inhibitory Synapses ◽  
Nucleotide Exchange

EPAC2 is a guanine nucleotide exchange factor that regulates GTPase activity of the small GTPase Rap and Ras) and is highly enriched at synapses. Activation of EPAC2 has been shown to induce dendritic spine shrinkage and increase spine motility, effects that are necessary for synaptic plasticity. These morphological effects are dysregulated by rare mutations of EPAC2 associated with autism spectrum disorders. In addition, EPAC2 destabilizes synapses through the removal of synaptic GluA2/3-containing AMPA receptors. Previous work has shown that Epac2 knockout mice (Epac2-/-) display abnormal social interactions, as well as gross disorganization of the frontal cortex and abnormal spine motility in vivo. In this study we sought to further understand the cellular consequences of knocking out Epac2 on the development of neuronal and synaptic structure and organization of cortical neurons. Using primary cortical neurons generated from Epac2+/+ or Epac2-/- mice, we confirm that EPAC2 is required for cAMP-dependent spine shrinkage. Neurons from Epac2-/- mice also displayed increased synaptic expression of GluA2/3-containing AMPA receptors, as well as of the adhesion protein N-cadherin. Intriguingly, analysis of excitatory and inhibitory synaptic proteins revealed that loss of EPAC2 resulted in altered of expression of vesicular glutamate transporter 1 (VGluT1) and vesicular GABA transporter (VGAT), indicating a potential imbalance in excitatory/inhibitory inputs onto neurons. Finally, examination of cortical neurons located within the anterior cingulate cortex further revealed subtle deficits in the establishment of dendritic arborization in vivo. These data provide evidence that EPAC2 is required for the correct composition of synapses and that loss of this protein could result in an imbalance of excitatory and inhibitory synapses.

scholarly journals Synaptic Activity-Dependent Changes in the Hippocampal Palmitoylome

2021 ◽  
Author(s):  
Glory Nasseri ◽  
Nusrat Matin ◽  
Kira Tosefsky ◽  
Richard Greg Stacey ◽  
Stephane Flibotte ◽  
...  
Keyword(s):  
Fear Conditioning ◽  
Ampa Receptors ◽  
Protein S ◽  
Postsynaptic Membrane ◽  
Synaptic Activity ◽  
Synaptic Proteins ◽  
Full Characterization ◽  
Metabolic Functions ◽  
Synapse Plasticity ◽  
Activity Dependent

Dynamic protein S-palmitoylation is critical for neuronal function, development, and synaptic plasticity. Activity-dependent changes in palmitoylation have been observed for several neuronal substrates, however a full characterization of the activity-regulated palmitoylome is lacking. Here, we use an unbiased approach to identify differentially palmitoylated proteins in the mouse hippocampus following context-dependent fear conditioning. Of the 121 differentially palmitoylated proteins identified 63 were synaptic proteins, while others were associated with metabolic functions, cytoskeletal organization, and signal transduction. The vast majority of synaptic proteins exhibited increased palmitoylation following fear conditioning, whereas proteins that exhibited decreased palmitoylation were predominantly associated with metabolic processes. We show a link between dynamic palmitoylation and synapse plasticity by demonstrating that the palmitoylation of one of our identified proteins, PRG-1/LPPR4, is essential for activity-induced insertion of AMPA receptors into the postsynaptic membrane. Together, this study identifies networks of synaptic proteins whose dynamic palmitoylation may play a central role in learning and memory.

Sign in / Sign up

Export Citation Format

Share Document